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Citations for Agilent :
5401 - 5450 of 7078 citations for Ceruloplasmin Colorimetric Activity Kit 2 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... The D614G amino acid change was introduced into VRC7480 by site-directed mutagenesis using the QuikChange Lightning Site-Directed Mutagenesis Kit from Agilent Technologies (Catalog # 210518) ...
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bioRxiv - Neuroscience 2021Quote: ... RNA integrity was assessed using the RNA Nano 6000 Assay Kit of the Bioanalyzer 2100 system (Agilent Technologies, CA, USA). A total amount of 3 µg RNA per sample was used as input material for the RNA sample preparations ...
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bioRxiv - Molecular Biology 2021Quote: ... RNA integrity and concentration were checked using the RNA Pico 6000 Assay Kit of the Bioanalyzer 2100 system (Agilent Technologies). Small RNA libraries were prepared from 1 ng of RNA using the NEXTflex Small RNA-seq Kit v3 (Perkin Elmer) ...
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bioRxiv - Molecular Biology 2020Quote: ... Site-directed mutagenesis to generate the miCLIP-mutants was performed using the QuikChange II Site-Directed Mutagenesis Kit from Agilent as per the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... Size-selected libraries were verified using the Agilent 2100 Bioanalyzer with the High Sensitivity DNA kit (Agilent, cat# 5067-4626) and quantified using KAPA Library Quantification Kits (KAPA Bio ...
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bioRxiv - Molecular Biology 2021Quote: RNA purified from polysomal fractioning (the reconstituted total mRNA and pooled polysomal RNA) was tested for quality using a RNA 6000 nano kit (Agilent) on a Bioanalyzer 2100 (Agilent).
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bioRxiv - Molecular Biology 2021Quote: ... HRP-conjugated secondary antibodies were then incubated on slides for 1-hour and detected with the DAB+ chromogen kit (DAKO). After washing ...
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bioRxiv - Molecular Biology 2020Quote: ... 154 mutated to leucine) was generated using the QuickChange Site Directed Mutagenesis Kit according to the manufacturer’s protocol (Agilent #200519). The BirA Ligase containing plasmid was purchased (Addgene #64395) ...
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bioRxiv - Molecular Biology 2020Quote: The plasmid encoding NusG-F165A was generated by site-directed mutagenesis according to the QuikChange Site-Directed Mutagenesis Kit (Stratagene), using vector pET11A_nusG (Burmann et al. ...
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bioRxiv - Cell Biology 2022Quote: ... of hPASMC were measured using Seahorse XF96 metabolic analyzer and Seahorse XF Cell Mito Stress Test kit (103015-100, Seahorse XF Technology, Agilent), following manufacturer’s recommendations ...
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bioRxiv - Immunology 2022Quote: ... Other variants containing single or multiple amino-acid changes in the spike gene from wildtype or D614G were made by mutagenesis using QuickChange lightning Multi Site-Directed Mutagenesis Kit (cat # 210515, Agilent) or via synthesis and cloning (Genscript) ...
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bioRxiv - Genomics 2020Quote: ... the amplified libraries were purified using a Qiagen MinElute PCR Purification Kit and eluted in 20 μl Elution Buffer before quantitation on an Agilent 4200 TapeStation (Agilent Technologies LDA UK Limited ...
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bioRxiv - Genomics 2019Quote: ... The sheared DNA was used to prepare indexed whole exome sequencing libraries using the Agilent SureSelect XT Human all exon v6 + UTR kit (Agilent Technologies ...
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bioRxiv - Genomics 2020Quote: ... RNA quality was assessed using the RNA Nano 6000 Assay Kit of the Bioanalyzer 2100 system (Agilent Technologies, CA, USA) and was checked on RNase free agarose gel electrophoresis ...
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bioRxiv - Immunology 2021Quote: ... Extracted RNA samples were then quantified and qualified using a Bioanalyzer 2100 and RNA 6000 Nano LabChip Kit (Agilent, USA) with RIN number >7.0 ...
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bioRxiv - Molecular Biology 2021Quote: ... Quality of cDNA libraries were studied with BioAnalyzer DNA analysis (5067-1504, Agilent, Agilent High Sensitivity DNA kit, 5067-4626). Final DNA concentration was determined with Quantus Fluorometer (E6150 ...
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bioRxiv - Molecular Biology 2021Quote: ... Mutant Mul DNA gyrases (Ala91Val or Asp95Gly) were generated from WT gyrA and gyrB using a QuickChange site-directed mutagenesis kit (Agilent Technologies ...
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bioRxiv - Molecular Biology 2021Quote: ... Final and cDNA libraries were then QCed on a bioanalyzer (Bioanalyzer High Sensitivity DNA kit: Agilent Technologies, Inc., 5067-4626) and qubit (dsDNA high sensitivity kit ...
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bioRxiv - Cell Biology 2020Quote: ... substitution of Cysteine to Alanine at 548 in Py-S2 was introduced using a site-directed mutagenesis QuikChangeII kit (Agilent) by following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: Constructs for BaxT182I and G179P were generated with a QuickChange site-directed mutagenesis kit (#210518; Agilent Technologies, Santa Clara, CA) on the pTYB1 WT-human Bax plasmid (Suzuki et al. ...
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bioRxiv - Genomics 2019Quote: The mutagenesis reactions to remove the Adenosine nucleotides near the cleavage sites were carried out using the QuikChange Site-Directed Mutagenesis Kit (Agilent). The mutagenesis DNA primers for the site mutation reactions are available in Supplemental Table S2 ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... at 4 °C overnight and immunostained using the streptavidin-biotin peroxidase technique (Envision universal peroxidase kit; Dako Cytomation, Milan, Italy). After incubation ...
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bioRxiv - Cancer Biology 2019Quote: ... were quantified using KAPA library quantification kits (KAPA) and fragment size distributions were determined using a Bioanalyzer or Tapestation (Agilent). Sequencing was performed on a HiSeq 4000 (Illumina).
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bioRxiv - Plant Biology 2020Quote: ... Library validation included size and purity assessment with the Agilent 2100 Bioanalyzer and the Agilent DNA 1000 kit (Agilent Technologies). Samples were diluted to 10nM and pooled to create the 12X multiplexed DNA libraries ...
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bioRxiv - Biochemistry 2019Quote: ... RNA integrity was assessed using the RNA Nano 6000 Assay Kit of the Bioanalyzer 2100 system (Agilent Technologies, CA, USA). The RNA concentration was measured with an RNA Assay Kit in Qubit 2.0 Flurometer (Life Technologies ...
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bioRxiv - Microbiology 2019Quote: ... the kaiC36803 gene in the pGEX-kaiC36803 vector was modified by site directed mutagenesis using the Quick-Change Site-Directed Mutagenesis Kit (Stratagene) or Q5 Site-Directed Mutagenesis Kit (New England Biolabs) ...
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bioRxiv - Cell Biology 2019Quote: ... Agilent gene expression microarray 60K slide (Design ID: 72363. SurePrint G3 Human Gene Expression 8 × 60K Microarray Kit, Agilent Technologies) were used ...
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bioRxiv - Developmental Biology 2019Quote: ... Mutations in the miR-204 binding sites were generated using the QuikChange site-directed mutagenesis kit (Stratagene, La Jolla, CA) and the mutated sequences were confirmed by DNA sequencing ...
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bioRxiv - Genomics 2020Quote: ... Final library concentration was assessed by Qubit dsDNA HS Assay Kit (Thermo-Fischer Scientific) and fragment size was checked using Tapestation High Sensitivity D1000 (Agilent) to ensure that fragment sizes were distributed normally about 500 bp ...
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bioRxiv - Cell Biology 2019Quote: ... All the mutant versions of recombinant proteins were produced by QuickChange Mutagenesis kit to mutate the plasmid DNA (Agilent Technologies). The MAD1 fragments and Cyclin B1 were transformed into BL21(DE3 ...
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bioRxiv - Microbiology 2019Quote: ... Fragment size distribution was analyzed by capillary gel electrophoresis using Agilent High Sensitivity DNA kit in a Bioanalyzer 2100 instrument (Agilent) then indexed sequencing libraries were prepared using TruSeq Nano DNA LT kit (Illumina ...
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bioRxiv - Genomics 2020Quote: ... The quality of the library was checked on DNA high sensitivity assay kit on Bio-analyser 2100 (Agilent Technologies, USA) and were sequenced on the Ion S5 Plus sequencing platform using 530 chip.
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bioRxiv - Pharmacology and Toxicology 2020Quote: Deletion and site-directed mutants were generated in human ENaC cDNAs cloned into a pGEM HE vector using the QuikChange II Site-Directed Mutagenesis kit (Stratagene) (Ji & Benos ...
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bioRxiv - Cancer Biology 2020Quote: ... The DNAJB1-PRKACA K128H kinase-dead mutant was cloned using the QuikChange II XL Site-Directed Mutagenesis Kit (Agilent Technologies) using the following primers (5’-CCTTCTGTTTGTCGAGGATATGCATGGCATAGTGGTTCCCG-3’ and 5’-CGGGAACCACTATGCCATGCATATCCTCGACAAACAGAAGG-3’) ...
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bioRxiv - Developmental Biology 2019Quote: Pkin-29::kin-29SER517ALA was generated by modifying Pkin-29::kin-29cDNA using PCR-based mutagenesis (Quickchange II XL site-directed mutagenesis kit, Stratagene). The following primers were used ...
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bioRxiv - Molecular Biology 2020Quote: ... the gene of FASTD71V,P73T was randomly mutagenized by error-prone PCR using the GeneMorph II Random Mutagenesis Kit (Agilent). The PCR product was digested with NheI and BamHI ...
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bioRxiv - Cell Biology 2019Quote: ... The HsMyo1e E337S TEDS site mutation was generated in pBS-HsMyo1e construct using the QuickChange kit (Stratagene; La Jolla, CA) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2019Quote: ... Isolated RNA was reverse transcribed into cDNA was by Prime Script TM RT reagent Kit with gDNA Eraser (Stratagene, Takara). Real-time PCR was performed on ABI-7500 real-time PCR machine (Applied Biosystems ...
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bioRxiv - Microbiology 2019Quote: ... For quality control libraries were analyzed with a DNA High Sensitivity Kit on a 2100 Bioanalyzer system (Agilent Technologies, USA) and again quantified on a Quantus™ Fluorometer (Promega ...
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bioRxiv - Cell Biology 2019Quote: ... The KEN-box mutant was generated in the pMT-Cyclin B1-GFP plasmid using a site-directed mutagenesis kit (Agilent), following the manufacturer instructions ...
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bioRxiv - Biochemistry 2020Quote: Desired mutations were introduced into mhsT wild-type gene via site-directed mutagenesis (Mutagenesis QuikChange Lightning Kit, Agilent Technolgies, Inc) using designed primers ...
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bioRxiv - Microbiology 2019Quote: ... Spike-in RNAs were added to 100 ng total RNA using the One-color RNA Spike-In kit (Agilent Technologies) and Cy3-labeled cRNAs were synthesized using the Low Input Quick Amp Labeling kit (one-color ...
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bioRxiv - Developmental Biology 2019Quote: ... The total RNA quantity and purity were analyzed on Bioanalyzer 2100 and RNA 6000 Nano LabChip Kit (Agilent, CA, USA) with RIN number >7.0 ...
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bioRxiv - Systems Biology 2020Quote: ... After double XP beads purifications (with beads added in a ratio 1:1) libraries were quantified on a Fragment Analyzer run with a NGS Assay Kit (Agilent) and loaded on a HiSeq 3000 flowcell with 50 cycles single end sequencing by pooling the samples based on molarity aiming for 30 mio ...
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bioRxiv - Cancer Biology 2019Quote: ... The RCC1 domain was deleted using the Quickchange® II XL Site-Directed Mutagenesis Kit according to manufacturer’s instructions (Stratagene) using the primer sequences ...
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bioRxiv - Biophysics 2019Quote: ... The γS N14D and γS N76D were generated via site-directed mutagenesis using QuikChangeXL Kit (Agilent Technologies, Santa Clara, CA). Escherichia coli cell stocks containing the plasmid of interest were grown at 37 °C ...
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bioRxiv - Developmental Biology 2019Quote: ... were dissociated and 106 cells were used to extract total RNA following manufacturer’s instructions of the Absolutely RNA Miniprep Kit (#400800, Agilent Technologies). cDNA was synthesised with 200 ng-1 μg of total RNA using M-MLV Reverse Transcriptase (Invitrogen ...
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bioRxiv - Molecular Biology 2019Quote: Constructs for the ScSth1p bromodomain mutants (Ser1276Glu, His1272Ala, Trp1338Gln, or Tyr1290Ala/Asn1333Ala) were generated using QuikChange site-directed mutagenesis kit (Stratagene). Mutant bromodomain expression and purification were carried out as for the wild-type protein ...
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bioRxiv - Molecular Biology 2019Quote: ... m6A-mut CIRBP reporter was generated by mutating the essential C in the m6A site synonymously to T using two rounds of site-directed mutagenesis with the QuikChange Lightning kit (Agilent).
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bioRxiv - Genomics 2019Quote: ... Library preparation and target enrichment was carried out using the SureSelect XT Mouse All Exon Kit (Agilent cat.# 5190-4641) per manufacturer’s instructions ...