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Citations for Agilent :
5051 - 5100 of 6813 citations for Medium Kit without Serum and with CultureBoost R since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... RNA integrity was assessed with 1µl of each mRNA-purified sample using RNA 6000 Pico kit (Agilent, 5067-1513) on an Agilent Bionalyzer with RNA Integrity Number (RIN ...
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bioRxiv - Molecular Biology 2024Quote: ... Two µL of each diluted sample were run using the HS Large Fragment 50kb Kit #DNF-464-0500 (Agilent) according to the manufacturer’s instructions on the 5200 Fragment Analyzer System # M5310AA ...
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bioRxiv - Biophysics 2024Quote: ... The resulting WGBS libraries were evaluated on a 2100 Bioanalyzer using Agilent High-Sensitivity DNA Kit (Agilent #5067-4626) and quantified via qPCR using the KAPA Library Quantification Kit (KAPA #KK4824) ...
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bioRxiv - Neuroscience 2024Quote: ... Quality and concentration of extracted RNA was assessed using a Tapestation 2100 with the High Sensitivity RNA kit (Agilent) for samples extracted form sorted somata and using a 2100 Bioanalyzer with the RNA 6000 Pico kit (Agilent ...
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bioRxiv - Molecular Biology 2024Quote: ... The Agilent QuikChange Primer Design Program and the QuikChange II Site-Directed Mutagenesis Kit (Agilent Technologies, Santa Clara, USA) were utilized to incorporate point mutations into the coding sequence of human GAT1 ...
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bioRxiv - Plant Biology 2020Quote: ... RNA quality and concentration of each sample were checked using an Agilent RNA 6000 Nano Kit Bioanalyzer chip (Agilent Technologies). Five micrograms of total purified RNA was enough for the reverse transcription reactions using the SuperScript III First-Strand Synthesis System for RT-PCR (Life Technologies) ...
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bioRxiv - Microbiology 2020Quote: ... Site-directed mutagenesis of the pCMV-S4 vector was performed using protocols and materials from QuickChange Lightning mutagenesis kit (Agilent). Nearly confluent monolayers of HEK293 cells in 12 well plates were transfected with either 0.5 μg of empty vector or 0.25 μg each of WT σ3 or σ3 K293T expression vector using 1.5 µl Lipofectamine 2000 according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2021Quote: Genes encoding EnHD and CHD in pET24 expression plasmids were described previously.(18) Single residue substitutions were introduced using a Quikchange site-directed mutagenesis kit (Agilent). Genes encoding sequences with multiple substitutions were synthesized by GeneArt (ThermoFisher Scientific ...
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bioRxiv - Genetics 2020Quote: Plamids for overexpression of the Nrd1 CID variants R133G and R133D were obtained using QuikChange site-directed mutagenesis kit (Stratagene).
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bioRxiv - Cell Biology 2021Quote: ... Hsc70D10N was generated by PCR-based site-directed mutagenesis using the QuikChange II XL Site-Directed Mutagenesis Kit (Agilent Technologies). Cyclin D1 KO was conducted by CRISPR/Cas9 genome editing (Xie et al. ...
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bioRxiv - Systems Biology 2020Quote: ... The resulting pure DNA and RNA fractions were quality assessed using agarose gel electrophoresis and Bioanalyser RNA 6000 Pico Kit (Agilent). Nucleic acid concentration was quantified using Qubit dsDNA HS Assay and Qubit RNA HS Assay Kit (Invitrogen) ...
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bioRxiv - Molecular Biology 2020Quote: The lyase deficient mutant of HMGA2 was generated by sequential site-directed mutagenesis (QuikChange II Site-Directed Mutagenesis Kit, Agilent) of the first three arginines in the third AT-Hook domain ...
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bioRxiv - Molecular Biology 2020Quote: ... plasmid by digestion with AscI and MIuI and mutagenesis for RTEL1K48R was performed using QuikChange Lightning Multi Site-Directed Mutagenesis Kit (Agilent). Cells were infected with lentiviruses expressing the indicated RTEL1 proteins and infected cells were selected with puromycin as previously described (Garzia et al. ...
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bioRxiv - Developmental Biology 2021Quote: ... Samples quantity (RNA Integrity Number = RIN) and quality were assessed using the Agilent RNA 6000 Pico Kit (Agilent #5067-1513) on the Bioanalyzer 2100 ...
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bioRxiv - Cell Biology 2020Quote: ... The pcDNA3-HA-Bbs5-S19A construct was prepared by mutating Ser19 of Bbs5 to alanine with pcDNA-HA-Bbs5-WT as template and site-directed mutagenesis kit (Stratagene). The primers were FWD1 (5′-CTTCGACGTGTCCGCACAACAGATGAAAAC-3′ ...
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bioRxiv - Cell Biology 2020Quote: ... Site directed mutagenesis was done using a QuikChange® Lightning Site Directed Mutagenesis kit (Agilent Technologies, Inc., Santa Clara, CA).
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bioRxiv - Cell Biology 2020Quote: Mito fuel flex test: Agilent Seahorse XF Mito Fuel Flex Test Kit was used as per the standard protocol by Agilent Seahorse (https://www.agilent.com/cs/library/usermanuals/public/XF_Mito_Fuel_Flex_Test_Kit_User_Guide%20old.pdf) ...
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bioRxiv - Cell Biology 2020Quote: ... mRNA were extracted and the quality of these samples was assessed (Agilent RNA 6000 Nano Kit Quick, Agilent Bioanalyzer 2100); RNA Integrity Number (RIN ...
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bioRxiv - Cell Biology 2020Quote: A library encoding ARHGAP36 isoform 2 mutants was created via error-prone PCR (epPCR) using the GeneMorph II Random Mutagenesis Kit (Agilent). To determine the optimal epPCR conditions for library generation ...
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bioRxiv - Cell Biology 2020Quote: ... pWZL-Hygro-HDAC4PAM-ER pCW-Hygro-HDAC4PAM were obtained by sub-cloning a mutagenized HDAC4 (QuikChange Site-Directed Mutagenesis Kit, Agilent) into the linearized empty backbones by a restriction-based approach ...
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bioRxiv - Cell Biology 2019Quote: ... Point mutations were made with complementary primers (listed below) in pTRCHisA-padi2 vectors using QuikChange II Site-Directed Mutagenesis Kit (Agilent).
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bioRxiv - Cell Biology 2020Quote: ... pEGFP-C1-tubbyCT R332H and pEGFP-C1-tubbyCT Y343F were generated using QuikChange II XL Site-Directed mutagenesis kit (Stratagene, Agilent Technologies ...
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bioRxiv - Immunology 2021Quote: ... RNA integrity was determined by bleach agarose gel electrophoresis (Aranda et al., 2012) and further analyzed on a Bioanalyzer 2100 using the RNA 6000 Nano LabChip Kit (Agilent Technologies Inc. ...
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bioRxiv - Immunology 2021Quote: ... and extracellular acidification rate (ECAR) were measured in MSCs using a Seahorse XF24 Extracellular Flux Analyzer and kits from Agilent Technologies (Santa Clara ...
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bioRxiv - Immunology 2021Quote: ... PCR products were ligated into pSCB-Amp/Kan using the StrataClone Blunt PCR Cloning Kit (Agilent Technologies, La Jolla, CA). The Spic promoter was cloned into pGL3-Basic (Promega ...
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bioRxiv - Genetics 2021Quote: ... Quality assessment and library size estimation of the individual libraries was done using an HS DNA kit (Agilent, #5067-4626) in a Bioanalyzer 2100 ...
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bioRxiv - Developmental Biology 2021Quote: RNA integrity was assessed on an Experion bioanalyzer using a Bio-Rad Experion RNA High-Sensitivity kit or an Agilent 2100 bioanalyzer using a Bioanalyzer RNA 6000 Pico assay (Agilent). RNA concentrations were measured using a Qubit 4 fluorometer with a Qubit high-sensitivity RNA assay.
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bioRxiv - Developmental Biology 2021Quote: ... or the Illumina TruSeq Stranded Total RNA Library Prep kit (reripes samples) and checked using a Fragment Analyzer System (Agilent) before multiplexing ...
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bioRxiv - Molecular Biology 2021Quote: ... after which quantification and qualification of purified band(s) were undertaken using the Agilent DNA 1000 kit and BioAnalyzer (Agilent). Illumina NGS was performed with 2×250 bp sequencing without fragmenting the amplicons ...
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bioRxiv - Evolutionary Biology 2020Quote: ... RNA probes were synthetized by cloning the DNA amplified region in the Strataclone PCR Cloning Kit (240205-5, Agilent Technologies) (hoxd12a ...
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bioRxiv - Biophysics 2020Quote: ... by introducing a stop codon at position 475 (aa 233–474) using the QuikChange XL site-directed mutagenesis kit (Stratagene). STIM1 fragment 344-449 (CAD ...
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bioRxiv - Developmental Biology 2020Quote: ... RNA integrity was assessed through the RNA 6000 Pico Assay Kit of the Bioanalyzer 2100 system (Agilent Technologies, CA, USA). A total amount of 3μg RNA per sample was used for mRNA purification and library construction via the Truseq™ RNA Sample Prep Kit (Illumina ...
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bioRxiv - Developmental Biology 2020Quote: ... Size distribution and concentration of the final products were then evaluated using the Bioanalyzer High Sensitivity DNA kit (Agilent Technologies). The libraries were pooled and diluted to 3 nM using 10 mM Tris-HCl ...
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bioRxiv - Genomics 2020Quote: ... and RNA 6000 Pico and RNA 6000 Nano kits –depending on the experiment– with a BioAnalyzer 2100 (Agilent Technologies Inc.). Libraries for each sample type were prepared using the TruSeq Stranded Total RNA Library Prep Kit (Illumina) ...
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bioRxiv - Cancer Biology 2022Quote: ... immunohistochemistry was performed by using the streptavidin-biotin-peroxidase method with diaminobenzidine as the chromogen (Kit LSAB, Dakocytomation, Glostrup, Denmark). Negative controls were obtained after omission of the primary antibody or incubation with an irrelevant antibody.
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bioRxiv - Molecular Biology 2022Quote: Concentrations were measured by Qubit RNA HS assay kit and the quality of RNA was analyzed on Tapestation D5000 HS (Agilent). Removal of rRNAs from isolated samples of IFNβ stimulated ESCs and MEFs was done following the protocol of Ribo-Zero rRNA removal kit ...
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bioRxiv - Biochemistry 2022Quote: ... The various mutant forms of this plasmid were made with the QuikChange II XL Site-Directed Mutagenesis Kit from Agilent using the appropriate primers each time (Table S4) ...
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bioRxiv - Developmental Biology 2022Quote: ... Specific point mutations in Hmga1 3’UTRs (shown in Fig 3A) cloned into pRL-CMV vector were generated using site-directed mutagenesis kit (Stratagene). In addition ...
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bioRxiv - Cell Biology 2022Quote: ... K22M mutation of the kinase dead PMVK mutant (PMVKmut) was generated using QuickChange Site-Directed Mutagenesis Kit (Agilent, Catalog # 200518). Lentiviral particles were used to constitutively express shPMVK (pLV[shRNA]-Hygro-U6> hPMVK[shRNA#4] ...
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bioRxiv - Biochemistry 2022Quote: ... P286R and V411L variants were obtained by carrying out point mutation to these clones using the QuikChange II site directed mutagenesis kit (Agilent). The oligonucleotides used to produce these mutations are shown in Table 2 ...
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bioRxiv - Biochemistry 2022Quote: The mutations S26C and K28C were introduced to SD-Aβ40 fusion protein19 via site-directed mutagenesis using a QuikChange Lightning Kit (Agilent, USA) following a modified protocol.22 The successful introduction of mutations was confirmed by sequencing (Genewiz) ...
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bioRxiv - Molecular Biology 2019Quote: FANCJ point mutations were generated by site-directed mutagenesis using a Quick Change II XL Site-Directed Mutagenesis Kit (Agilent), according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... quality and quantity of both transcriptome and translatome samples were evaluated in an Agilent 2100 Bioanalyzer using Nano and Small RNA kits (Agilent Technologies ...
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bioRxiv - Biochemistry 2020Quote: ... The D614G amino acid change was introduced into VRC7480 by site-directed mutagenesis using the QuikChange Lightning Site-Directed Mutagenesis Kit from Agilent Technologies (Catalog # 210518) ...
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bioRxiv - Neuroscience 2021Quote: ... RNA integrity was assessed using the RNA Nano 6000 Assay Kit of the Bioanalyzer 2100 system (Agilent Technologies, CA, USA). A total amount of 3 µg RNA per sample was used as input material for the RNA sample preparations ...
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bioRxiv - Molecular Biology 2021Quote: ... RNA integrity and concentration were checked using the RNA Pico 6000 Assay Kit of the Bioanalyzer 2100 system (Agilent Technologies). Small RNA libraries were prepared from 1 ng of RNA using the NEXTflex Small RNA-seq Kit v3 (Perkin Elmer) ...
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bioRxiv - Molecular Biology 2020Quote: ... Site-directed mutagenesis to generate the miCLIP-mutants was performed using the QuikChange II Site-Directed Mutagenesis Kit from Agilent as per the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... Size-selected libraries were verified using the Agilent 2100 Bioanalyzer with the High Sensitivity DNA kit (Agilent, cat# 5067-4626) and quantified using KAPA Library Quantification Kits (KAPA Bio ...
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bioRxiv - Molecular Biology 2021Quote: RNA purified from polysomal fractioning (the reconstituted total mRNA and pooled polysomal RNA) was tested for quality using a RNA 6000 nano kit (Agilent) on a Bioanalyzer 2100 (Agilent).
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bioRxiv - Molecular Biology 2021Quote: ... HRP-conjugated secondary antibodies were then incubated on slides for 1-hour and detected with the DAB+ chromogen kit (DAKO). After washing ...