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Citations for Agilent :
5051 - 5100 of 8606 citations for Estriol ELISA Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... R256L and Y250L in AQP3 mutations were inserted using the QuikChange II kit (Agilent, Santa Clara, CA, USA), following the manufacturer’s instructions ...
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bioRxiv - Biophysics 2020Quote: ... Mutant libraries of CI2 WT and CI2 I57A were generated using the GeneMorph II Random mutagenesis kit (Agilent). The mutation frequency was aimed at 0-4 amino acid substitutions per gene by adjusting the initial target DNA and the number of amplification cycles ...
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bioRxiv - Neuroscience 2022Quote: ... Phospho-null mutation VASP S239A was generated using the QuikChange II Site-Directed Mutagenesis Kit (Cat.#200523, Agilent). Primers were designed using the QuikChange Primer Design Program and obtained from Integrated DNA Technologies IDT ...
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bioRxiv - Developmental Biology 2022Quote: ... Quality and yield of isolated RNA was assessed using the Eukaryotic Total RNA 6000 Nano kit (Agilent technologies) and the Queensland Brain Institute’s Bioanalyser 2100 (Agilent technologies) ...
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bioRxiv - Microbiology 2022Quote: ... Site-directed mutagenesis of these plasmids for C483A or Pol(-) mutants were obtained with the QuikChange kit (Stratagene) using primers listed in Supplementary Table 1 ...
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bioRxiv - Biochemistry 2022Quote: ... L452R and T478K for the δ variant) were introduced using the QuickChange Site-Directed Mutagenesis kit (Agilent Technologies) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... and isolated RNA was quantified and its quality was assessed using the RNA 6000 Pico Bioanalyzer kit (Agilent). PBMC RNA samples had RNA integrity number (RIN ...
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bioRxiv - Evolutionary Biology 2022Quote: Random mutagenesis of the hdp1 and hdp2 genes was performed with the GeneMorph II Random Mutagenesis Kit (Agilent) using the recommended conditions for obtaining a mean mutation rate of 1 mutation per PCR product ...
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bioRxiv - Immunology 2022Quote: ... Knockout PCR products were cloned into pSC-B-amp/kan with the StrataClone Blunt PCR Cloning Kit (Agilent) and Sanger sequenced to determine the exact deletion ...
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bioRxiv - Cell Biology 2022Quote: ... Mutations were added to wild-type H3.1-V5 and H3.3-V5 sequences using the Quikchange II XL site directed mutagenesis kit (Agilent). Lentiviruses were transfected into HEK293T cells in combination with viral packing and envelope plasmids pSPAX (Addgene 12260 ...
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bioRxiv - Zoology 2019Quote: ... boddarti Elovl5 sequence was carried out using the QuikChange site-directed mutagenesis kit (Agilent Technologies, La Jolla, CA) according to the manufacturer’s protocol with primers listed in Table 1 using the pGEM®-T Easy vector containing the B ...
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bioRxiv - Microbiology 2019Quote: ... Insert sizes and concentrations for each library were determined with an Agilent Bioanalyzer DNA 100 kit (Agilent Technologies). Libraries were finally sequenced on the Illumina NextSeq platform at the MIT Biomicro Center to generate 2 × 150 bp paired reads ...
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bioRxiv - Genomics 2019Quote: ... Small and long RNA quality was assessed using an Agilent 2100 Bioanalyzer and RNA 6000 nano kits (Agilent) and quantified on a Nanodrop spectrophotometer.
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bioRxiv - Developmental Biology 2019Quote: ... RNA quality was evaluated using Agilent Bioanalyzer with RNA 6000 Pico kit (Agilent Technologies Japan, Ltd., Tokyo, Japan). An amount of 500 ng total RNA was used for RNA-seq library preparation with NEB NEBNext rRNA Depletion Kit and ENBNext Ultra Directional RNA Library Prep Kit (New England Biolabs Japan Inc. ...
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bioRxiv - Neuroscience 2019Quote: Target enrichment by liquid-phase hybridization capture was performed using the SureSelect Mouse Methyl-Seq kit (Agilent Technologies) 42 ...
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bioRxiv - Pathology 2019Quote: ... RNA integrity was assessed using the RNA Nano 6000 Assay Kit of the Bioanalyzer 2100 system (Agilent Technologies). Library construction was done using NEBNext Ultra Directional RNA Library Prep Kit for Illumina (NEB ...
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bioRxiv - Microbiology 2019Quote: ... A site-directed mutant of Pnf (toxoid) was generated with either the QuikChange site-directed mutagenesis kit (Agilent). To construct the Pnf mutant plasmid pET15b-PnfC190A ...
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bioRxiv - Immunology 2019Quote: ... Baseline ECAR and changes in glycolytic rate were assessed using the Seahorse XFp Glycolytic Rate Assay Kit (Agilent) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2019Quote: ... RNA-seq libraries for control bulk RNA were generated using the SureSelect Strand-specific kit (Agilent Technologies, G9691B). Bulk DNA from over 1 × 105 cells was obtained from the cell culture (T0.5 sample ...
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bioRxiv - Plant Biology 2019Quote: ... Site-directed mutagenesis was carried out using the QuikChange Lightning Multi Site-Directed Mutagenesis Kit (Agilent, CA, USA) according to manufacturer's instructions.
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bioRxiv - Molecular Biology 2019Quote: ... The average size and concentration of libraries were determined using the 2100 Bioanalyzer High Sensitivity DNA Kit (Agilent) and qPCR with SensiMix SYBR (Bioline ...
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bioRxiv - Cell Biology 2019Quote: ... the pMTT1-GFP-LF4A plasmid was subjected to site-directed mutagenesis using the QuikChange Lightning kit (Agilent 210518) with the primers 5’-CAGGACGTTTGGCACTAGTGGCTGAATTGATGGATCAGAACC-3’ and 5’-GGTTCTGATCCATCAATTCAGCCACTAGTGCCAAACGTCCTG-3’.
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bioRxiv - Biochemistry 2019Quote: ... coli GyrB overexpression was mutated by site directed mutagenesis using the QuikChange XL Site-Directed Mutagenesis kit (Agilent) in order to generate two plasmids harboring R286K or R286Q mutations ...
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bioRxiv - Molecular Biology 2020Quote: ... Preparations were analyzed using an Agilent 2100 Bioanalyzer and Agilent Small RNA kit (Agilent Technologies, Santa Clara CA).
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bioRxiv - Microbiology 2020Quote: ... pT7/EV-A71BrCr and pCMV/hRV14 by using the QuikChange II XL Site-Directed Mutagenesis Kit (Agilent Technologies). All mutants were verified by sequencing after mutagenesis ...
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bioRxiv - Physiology 2021Quote: ... RNA integrity was verified using an Agilent Technologies 2100 Bioanalyzer with the RNA 6000 Nano Kit (Agilent Technologies). RNA quantity was determined using a Nanodrop ND-1000 spectrophotometer (PEQlab Biotechnologie GmbH) ...
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bioRxiv - Molecular Biology 2020Quote: ... L765A) of this fragment were cloned from this vector using a QuikChange II site-directed mutagenesis kit (Agilent). The DNA construct used for proteins for crystallography was similar except it coded for PARP-1 662-1011 (again with V762A ...
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bioRxiv - Neuroscience 2020Quote: ... PLCγ2-myc-DDK was subjected to site-directed mutagenesis (QuikChange Lightning Multi Site-Directed Mutagenesis Kit, Agilent, 210515) to create PLCγ2-P522R-myc-DDK (P522R ...
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bioRxiv - Biophysics 2021Quote: ... and L207W mutations were generated by performing site-directed mutagenesis PCR using Quik Change II Mutagenesis Kit (Agilent) with the following primers:
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bioRxiv - Plant Biology 2019Quote: ... which was Cy3 labelled via the Low Input Quick Amp Labeling Kit (Agilent Technologies, Santa Clara, CA, USA). The labelling efficiency ...
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bioRxiv - Synthetic Biology 2020Quote: ... The assembly products were then re-purified using the Monarch PCR & DNA Cleanup Kit and quantified by Agilent Bioanalyzer (DNA 1000).
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bioRxiv - Molecular Biology 2021Quote: ... The quality of the RNA was assessed using the Agilent 6000 Pico kit with the Bioanalyzer 2100 (Agilent) (Figure S5) ...
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bioRxiv - Microbiology 2020Quote: ... DNA library quality control was performed using a Tapestation 2200 with a High Sensitivity D1000 kit (Agilent Technologies) according to the manufacturer’s instructions.
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bioRxiv - Plant Biology 2020Quote: ... Different point mutations of cysteines to serines in GRXS17 were generated using QuikChange II Directed Mutagenesis Kit (Agilent) using primers detailed in Supplemental Table 2 ...
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bioRxiv - Biochemistry 2020Quote: The T2X mutations (T2A, T2R, T2Q and T2S) were generated by site-directed mutagenesis (Agilent Quick Change Kit) using the pMH406 plasmid containing esxB-esxA operon as a template ...
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bioRxiv - Genetics 2020Quote: ... The quality of the purified DNA library was assessed by a Bioanalyzer High-Sensitivity DNA Analysis Kit (Agilent). Illumina HiSeq SBS Kit v4 was used for PE101 sequencing (Illumina).
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bioRxiv - Molecular Biology 2019Quote: ... The obtained libraries were validated and quantified by an Agilent 2100 Bioanalyzer using a DNA7500 LabChip kit (Agilent) and an equimolecular pool of libraries was prepared and titrated by qRT-PCR using the “Kapa-SYBR FAST qPCR kit forLightCycler480” (Kapa BioSystems ...
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Structure and mechanism of a Type III CRISPR defence DNA nuclease activated by cyclic oligoadenylatebioRxiv - Biochemistry 2019Quote: ... R206E and R249E were generated using the QuikChange Site-Directed Mutagenesis kit as per manufacturer’s instructions (Agilent Technologies). The pEV5HisTEV-can1 wild-type and variant constructs were transformed into C43 (DE3 ...
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bioRxiv - Evolutionary Biology 2019Quote: ... RNA integrity was assessed using Agilent RNA 6000 Nano kit and 2100 Bioanalyzer instrument (both Agilent Technologies, USA). Both fractions were depleted from ribosome RNA using the Ribo-Zero depletion Kit for Gram negative bacteria (Illumina ...
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bioRxiv - Molecular Biology 2019Quote: ... RNA quality was examined using an Agilent RNA 6000 Nano kit with an Agilent 2100 Bioanalyzer (Agilent Technologies). Two μg of RNA was reverse-transcribed with 0.5 μg oligo(dT)18 primer using an Applied Biosystem™ High-Capacity cDNA Reverse Transcription kit (ThermoFisher Scientific) ...
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bioRxiv - Molecular Biology 2019Quote: ... The R1285Q mutant of human FANCI (FANCImut) was generated by using QuickChange Lightning Site-Directed Mutagenesis Kit (Agilent), and was cloned with a N-terminal cleavable Flag tag into the pCDNA 3.1 vector ...
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bioRxiv - Genetics 2019Quote: ... and exome DNA was enriched with an Agilent SureS elect Human All Exon V5 kit (Agilent Technologies, USA). Paired-end sequencing was performed on Illumina HiSeq 4000 (Supplementary Table 1 and 2).
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bioRxiv - Genomics 2021Quote: ... and quantified by qPCR using the KAPA Library Quantification Kit (KapaBiosystems) on a MxPro instrument (Agilent Technologies, USA). The libraries were sequenced using 251 base- length read chemistry in a paired-end flow cell on the Illumina HiSeq2500 sequencer (Illumina ...
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bioRxiv - Genetics 2021Quote: ... RNA integrity was assessed with the RNA Nano 6000 assay kit and the Bio analyzer 2100 system (Agilent).
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bioRxiv - Genetics 2020Quote: ... Mutations were introduced by site-directed mutagenesis of the wild-type gene using the QuikChange mutagenesis kit (Agilent). Sequencing was performed to confirm correct base pair substitutions or deletions were made.
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bioRxiv - Physiology 2021Quote: Mutations were introduced into human Cx50 cDNA in a SP64T vector with QuikChange Site-Directed Mutagenesis Kit (Stratagene) by using PCR primers that amplified the whole plasmid ...
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bioRxiv - Immunology 2020Quote: cDNA was synthesized by using the AffinityScript One-Step RT-PCR Kit (Cat# 600559, Agilent, Santa Clara, US) using extracted RNA ...
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bioRxiv - Biochemistry 2020Quote: Point mutations in ABCB1 were introduced into 35S:ABCB1-YFP (26) and 35S:ABCB14-GFP (23) using the QuikChange Lightning Site-Directed Mutagenesis Kit (Agilent).
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bioRxiv - Neuroscience 2021Quote: ... and AlixΔPGY (AlixΔALG-2) cDNAs in pCI were generated by mutagenesis (Quick change II site directed mutagenesis kit, Stratagene) and Alix R757E (AlixΔendo ...
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bioRxiv - Neuroscience 2021Quote: ... The resulting cDNA presented a fragment size distribution of 1,500 up to 5,000 bp on the Bioanalyzer High Sensitivity DNA Kit (Agilent) and a concentration above 300 pg/µl measured with Qubit dsDNA HS fluorometric quantification (ThermoFisher Scientific) ...