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Citations for Agilent :
451 - 500 of 1478 citations for 5 Methylsulfamoylmethyl 1H indole 3 carboxylic acid methyl ester since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... and integrity from 3 independent replicates were checked using the 4195 Tapestation system (Agilent).
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bioRxiv - Cancer Biology 2024Quote: Cells were washed 3 times with 100 µL of XF Base Media (Seahorse Bioscience) containing 2 mM L-Glutamine ...
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bioRxiv - Bioengineering 2024Quote: ... 3 technical replicates were assayed using the AriaMx Real-Time PCR System (Agilent Technologies) and 2×Taq Pro Universal SYBR qPCR Master Mix (Q712-02 ...
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bioRxiv - Cancer Biology 2024Quote: ... labeled with Cyanine 3-CTP using a Low Input Quick Amp Labeling Kit (Agilent), and hybridized to an SurePrint G3 Mouse GE Microarray (Agilent) ...
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bioRxiv - Microbiology 2020Quote: ... The 75th amino acid in the PB1-F2 protein was changed to a histidine (H) via site directed mutagenesis (QuikChange Lightning, Agilent) to produce the PB1-F2-75H plasmid ...
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bioRxiv - Plant Biology 2020Quote: ... and fluorenylmethoxycarbonyl (FMOC) was based on the application note “Automated amino acids analysis using an Agilent Poroshell HPH-C18 Column” by Agilent. The samples were injected onto a 100 mm x 3 mm InfinityLab Poroshell HPH-C18 column (2.7 μm ...
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bioRxiv - Cell Biology 2020Quote: Samples from all biological replicates were first sent to the Stanford University Protein and Nucleic Acid Facility (Stanford, CA) for quantification and quality analysis using a 2100 Bioanalyzer (Agilent). Samples were then sent to Novogene Corporation Inc ...
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bioRxiv - Biochemistry 2020Quote: ... The D614G amino acid change was introduced into VRC7480 by site-directed mutagenesis using the QuikChange Lightning Site-Directed Mutagenesis Kit from Agilent Technologies (Catalog # 210518) ...
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bioRxiv - Immunology 2020Quote: ... The codon encoding residue 241 of Copa was mutated from glutamic acid to lysine via Quikchange Lightning site directed mutagenesis (Agilent). Following NotI and PacI digestion ...
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bioRxiv - Microbiology 2020Quote: ... Hydrolyzed amino acids were separated using ultra performance liquid chromatography (UPLC, Acquity, Waters) on a C-8 column (Zorbax Eclipse XBD, Agilent) at a flow rate of 0.6 mL/min ...
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bioRxiv - Biochemistry 2020Quote: E.coli expression plasmids encoding GST-PEX14-NTD with amino acid substitutions were constructed via Site Directed Mutagenesis using QuikChange II (Stratagene). ß-tubulin (amino acids 388–444 ...
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bioRxiv - Bioengineering 2020Quote: ... rehydrated slides were subject to antigen retrieval using citric acid buffer at 120°C and 20 psi in a pressure cooker (DAKO). Afterwards ...
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bioRxiv - Biophysics 2020Quote: ... Chromatographic separation of OPA-derivatized amino acids was performed using an Agilent ZORBAX Eclipse AAA column (4.6mm x 150 mm x 3.5 μm; Agilent #963400-902) coupled with a ZORBAX Eclipse AAA Analytical Guard Column (4.6 mm x 12.5 mm x 5 μm ...
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bioRxiv - Microbiology 2021Quote: Levels of volatile fatty acids present in the supernatant of both co-cultures and monocultures were measured using an Agilent1260 Infinity HPLC (Agilent). Samples were prepared by acidifying to 5 mM using sulfuric acid and subsequently incubating at room temperature for 5 minutes ...
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bioRxiv - Microbiology 2020Quote: ... The 13C-labeling patterns of proteinogenic amino acids were determined on a 6890N Network GC system with a 5975 inert XL mass selective detector (Agilent Technologies Inc. ...
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bioRxiv - Immunology 2022Quote: ... The D614G amino acid change was introduced into VRC7480 by site-directed mutagenesis using the QuikChange Lightning Site-Directed Mutagenesis Kit from Agilent Technologies (catalog no ...
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bioRxiv - Immunology 2021Quote: ... we changed three amino acids within the Fc region by using the QuikChange II site directed mutagenesis kits (Agilent, #200523), introducing M257Y ...
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bioRxiv - Neuroscience 2021Quote: ... TDP-43N-Del was generated by deletion of the first 81 amino acids from the N-terminus of TDP-43WT using the QuickChange Site-Directed Mutagenesis Kit (Agilent).
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bioRxiv - Molecular Biology 2020Quote: The conservative lysine at position 23 was replaced with glutamic acid to create Meier-Gorlin mutation (K23E) following site-directed mutagenesis protocol (Agilent). Drosophila-Human and Human-Drosophila hybrids were designed by using a PCR technique ...
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bioRxiv - Plant Biology 2021Quote: Salicylic acid (SA) was extracted from frozen leaf material and analyzed by ultra-high-performance liquid chromatography system (Agilent Technologies) coupled to an Agilent 6530 quadrupole time of flight-mass spectrometer (Agilent Technologies ...
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bioRxiv - Cell Biology 2020Quote: The mCherry-TRAK1 deletion mutant mCherry-TRAK1Δ was obtained by inserting a stop codon after amino acid 635 of the mCherry-TRAK1 encoding nucleotide sequence by means of a PCR-based mutagenesis (Agilent Technologies ...
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bioRxiv - Microbiology 2020Quote: ... The GFP-tagged N127143Q mutant was constructed by substitution of asparagines for glutamines at amino acids 127 and 143 by site-directed mutagenesis (Stratagene). The GFP-Rem-T7 partial deletion mutants (GFP-RemΔ103-155-T7 ...
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bioRxiv - Cell Biology 2022Quote: ... Peptides were acidified by 1% trifluoroacetic acid (TFA) and C18 cleanup was performed using Bravo AssayMAP robot with C18 cartridges (Agilent) following the manufacturer’s instruction ...
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bioRxiv - Microbiology 2022Quote: ... The libraries were submitted to the VCU’s Nucleic Acid Sequencing core facility where the quality of the libraries was verified using the bioanalyzer (Agilent Technologies).
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bioRxiv - Physiology 2023Quote: ... Yttrium concentrations were determined in diets and feces after an acid digestion with HNO3 and measured with a coupled plasma-mass spectrometry (Agilent 4200 MP-AES ...
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bioRxiv - Physiology 2022Quote: ... This construct has an artifactual amino acid change in its coding sequence (I143V) and site-directed mutagenesis (Quikchange II XL, Agilent) was performed to convert it back to WT with following two primers (all primers were ordered from Integrated DNA Technologies):
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bioRxiv - Molecular Biology 2022Quote: ... Free fatty acids were separated on a Zorbax StableBond aq column (dimension: 2.1mm x 50mm, particle size: 1.7µm) (Agilent technologies, 827700–914) at 0.6ml/min using a mobile phase solvent consisted of 0.06% acetic acid in water (A ...
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bioRxiv - Neuroscience 2024Quote: ... Point amino acid substitutions in genes encoding GluN subunits were performed using the QuikChange site-directed mutagenesis kit (Agilent Technologies) and verified by DNA sequencing.
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bioRxiv - Biochemistry 2023Quote: ... The resulting peptide mixture was acidified by addition of 1% trifluoroacetic acid (TFA) and desalted on a reversed phase (RP) C18 OMIX tip (Agilent). The tip was first washed 3 times with 200 µl pre-wash buffer (0.1% TFA in water/acetonitrile (ACN ...
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bioRxiv - Immunology 2023Quote: ... stopped with Stop Solution (acid) and absorbance values were recorded at 450 nm on a spectrophotometer (using a BioTeck Gen5 plate reader (Agilent).
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bioRxiv - Microbiology 2023Quote: Binding of VHHs to spike proteins on the surface of mammalian cells was determined by flow cytometry using pcG1-expression plasmids containing the coding sequence of the SARS-CoV-2 spike protein from which the C-terminal 18 amino acids were deleted and in which the D614G substitution was introduced by QuickChange site-directed mutagenesis (Agilent) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: The substitution of amino acids via SDM was carried out using the QuikChange II Site-Directed Mutagenesis Kit (Agilent Technologies) per the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: The rate of fatty acids oxidation was determined in 30 days old midbrain organoids following Seahorse XF Mito fuel flex test protocol (Agilent) and using Seahorse XFe96 Spheroid FluxPak (Agilent ...
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bioRxiv - Cell Biology 2024Quote: ... were designed to replace the relevant amino acid residue with alanine and carried out using the PfuUltra II Fusion HS DNA Polymerase kit (600670, Agilent) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: The site-directed mutants of PtPelA and PaPelA without its predicted signal sequence (amino acids 47-948 for PaPelA; accession Q9HZE4) were generated using the QuikChange Lightning site-directed mutagenesis kit (Stratagene). The sequence of all vectors was confirmed using DNA sequencing.
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bioRxiv - Microbiology 2024Quote: ... Plasmids pLB21 and pLB24 were used to further exchange the minD isoleucine 260 to glutamic acid (I260E) by QuickChange site-directed mutagenesis reaction (Stratagene) with oligonucleotide pair HS205/HS206 ...
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bioRxiv - Pathology 2024Quote: Twenty to fifty grams of frozen caecal contents were used to analyzed the short-chain fatty acids (SCFAs) composition using gas chromatography (Agilent 7890B gas chromatograph ...
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bioRxiv - Microbiology 2024Quote: ... Reaction was quenched with 2 M sulfuric acid and absorbance at 450 nm was detected using a BioTek Synergy H1 microplate reader (Agilent). The assay was performed in duplicate ...
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bioRxiv - Neuroscience 2024Quote: The fatty acid composition of the plasma samples was analyzed by gas chromatography coupled with a flame ionization detector (model 6890; Agilent). 1 µl of the sample was injected in splitless mode at 250 °C ...
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NR2F2 Reactivation in Early-life Adipocyte Stem-like Cells Rescues Adipocyte Mitochondrial OxidationbioRxiv - Physiology 2024Quote: ... 1µL of pentafluorobenzyl-fatty acid derivatives was injected and data were collected on the GC-MS (8890 GC, 5977B MSD, Agilent) DB-1MS UI column (122-0112UI ...
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bioRxiv - Genetics 2024Quote: ... the reaction was stopped with the addition of 50 µL of sulfuric acid (1N) and the absorbance measured within 15 min at 450 nm using the Biotek Epoch Gen5 spectrophotometer (Agilent). The unknown aldosterone concentrations were extrapolating from the standard curve generated by the Gen5 software (v.2.09) ...
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bioRxiv - Biochemistry 2024Quote: ... a custom Agilent Personal Compound Database and Library (PCDL) of target metabolites (glycolytic and tricarboxylic acid cycle intermediates) was created from Agilent METLIN PCDL ...
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bioRxiv - Cell Biology 2024Quote: ... and bioenergetic profiles were measured with Real-Time ATP Rate Assay and Long Chain Fatty Acid (LCFA) Oxidation Stress Assay (Agilent). Concentrations of the used inhibitors were ...
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bioRxiv - Developmental Biology 2021Quote: ... and 5-μm sections were obtained and stained with hematoxylin (Dako) and eosin (VWR ...
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bioRxiv - Cancer Biology 2020Quote: ... Samples with RIN values > 5 as assessed by TapeStation (Agilent Technologies) were prepared with KAPA mRNA HyperPrep kit (Roche) ...
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bioRxiv - Molecular Biology 2022Quote: ... Data were collected on a Cytation 5 plate reader (Agilent Technologies) using a green fluorescent polarization filter (excitation and emission wavelengths of 485 nm and 528 nm ...
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bioRxiv - Neuroscience 2022Quote: ... Images were obtained using a Biotek Cytation 5 Multimode Reader (Agilent) to generate (at minimum ...
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bioRxiv - Immunology 2020Quote: ... FBXO10 E54K was generated by site-directed mutagenesis (Stratagene 200521-5) using the following primers:
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bioRxiv - Cell Biology 2022Quote: ... both solvents containing 5 µM Infinity Lab deactivator additive (Agilent Technologies). The elution gradient used was as follows ...
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bioRxiv - Bioengineering 2022Quote: ... with a 5-Å column (Agilent, 25m x 0.25mm x 30μm). Hydrogen that evolved during the BPEC stabilization stage (see previous section ...