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Citations for Agilent :
451 - 500 of 827 citations for 5 Alpha dihydroprogesterone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2021Quote: Quantitative analysis was performed using a HP-5MS capillary column (5% phenyl-methyl-siloxane, 30 m x 250 mm, 0.25 mm film thickness; Agilent) with helium carrier gas at 1.5 mL/min ...
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bioRxiv - Microbiology 2021Quote: ... samples (5 uL) were injected onto a ZORBAX RRHD Extend-C18 column (2.1 x 150 mm, 1.8 µm; Agilent Technologies) with a ZORBAX SB-C8 (2.1 mm × 30 mm ...
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bioRxiv - Neuroscience 2020Quote: ... and loaded onto a ZORBAX Extend 300 C18 columns (4.6 mm x 250 mm with 5 µm beads) from Agilent. An Agilent 1100 HPLC system was used to carry out the fractionation ...
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bioRxiv - Microbiology 2022Quote: ... containing peptaibols was carried out by semi-preparative HPLC on an SSI system (Teledyne SSI Lab Alliance Series III pump system and Series 1500 Photodiode Array Detector) with an Eclipse XDB-C18 column (9.4 × 250 mm, 5 μm, Agilent). Acetonitrile (A ...
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bioRxiv - Microbiology 2022Quote: ... After centrifugation plate was incubated for 5 minutes before being loaded into a Seahorse XF96 Extracellular Flux Analyzer (Agilent). Basal respiration was measured over six hours with 36 ten-minute protocol cycles including a ...
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bioRxiv - Biochemistry 2022Quote: ... Sample from each PNT3 variant at 5 mg mL-1 in buffer B containing 6M GDN was injected onto an AdvanceBio SEC 2.7 µm (Agilent) SEC column ...
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bioRxiv - Cancer Biology 2020Quote: ... and incubated with 1 × blocking buffer (5% goat serum [#X0907, Dako], 2.5% BSA, 0.1% Triton X-100 in PBS) (38) ...
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bioRxiv - Zoology 2020Quote: ... GC-SSR was performed on a GC-7890 Agilent Gas Chromatogram equipped with a DB-5 column (30 m, 0.25 mm; 0.25 μm; Agilent Technologies) and connected with a transfer line and a temperature control unit from Syntech ...
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bioRxiv - Biophysics 2020Quote: ... After rinsing for 5 minutes in PBS they were incubated with secondary antibody (Rabbit Anti-Mouse 1/200, Dako) for 30 minutes ...
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bioRxiv - Biochemistry 2020Quote: ... HPLC with DAD was performed on an Agilent 1200 Series instrument equipped with an Eclipse Plus C18 column (4.6 × 250 m; particle size = 5 μm; Agilent). Activities of the enzymes were estimated as Turnover Number nmoles of metabolized product / nmol of CYP / min (min-1) ...
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bioRxiv - Microbiology 2020Quote: ... 5 μL template DNA and 10 μL Brilliant III Ultra-Fast QPCR Master Mix (Agilent Technologies, Santa Clara, CA). PCR was conducted with a Stratagene Mx3005P (Agilent technologies ...
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bioRxiv - Neuroscience 2021Quote: ... Fusion products were digested with NotI and ligated to a NotI cleaved pAAV-MCS plasmid backbone (#240071-5, Stratagene). The final rAAV/SORL1 KO-Neo plasmid construct was verified by NotI digestions and sequencing ...
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bioRxiv - Biochemistry 2021Quote: ... Dried peptides were reconstituted in buffer A (0.1% FA) and injected into the trap column (Zorbax 300SB-C18, 0.3 5 mm; Agilent Technologies) at a flow rate of 0.18 μL/min in buffer A ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were blocked in 0.2% triton X-100 solution containing 1% BSA and 5% normal goat serum (DAKO, X0907) for 1 hour.
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bioRxiv - Cell Biology 2020Quote: ... 5 μm non-porous beads made of poly(styrene-divinylbenzene) modified with sulfonate groups (Agilent, Santa Clara, CA, USA).
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bioRxiv - Molecular Biology 2021Quote: ... The products were analysed by HPLC on a Shimadzu HPLC system (http://www.shimadzu.com) using a 5-lM C18 column (150 9 4.6 mm; Zorbax; Agilent, http://www.agilent.com). A linear gradient with increasing acetonitrile (solvent A ...
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bioRxiv - Plant Biology 2023Quote: ... A gradient elution system was optimized with the two mobile solvents to separate metabolites on an Eclipse XDB-C18 analytical column (250 × 4.6 mm, 5 µM, 25 ◦C, Agilent). The gradient solvent system was composed of ratios of solvent A to B ...
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bioRxiv - Cancer Biology 2022Quote: ... The material was loaded on to a Zorbax 2.1 x 150 mm (5 µm particle size) Extend-C18 column (Agilent) for basic reverse-phase fractionation ...
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bioRxiv - Microbiology 2023Quote: ... A large-inner diameter (i.d.) trapping column (300-μm i.d. x 5 mm, Agilent Technologies, Santa Clara, CA, USA) was coupled to the analytical column (75-μm i.d ...
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bioRxiv - Systems Biology 2023Quote: ... pH 8.5) and loaded onto an Agilent 300 Extend-C18 column (5 μm, 4.6 mm × 25 cm; Agilent Technologies). The peptides were then resolved using an increasing gradient of solvent B (10 mM TEAB in 90% acetonitrile (ACN) ...
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bioRxiv - Plant Biology 2023Quote: ... The LC separation was conducted on a Poroshell 120 EC-C18 analytical column (Agilent, 2.1 × 5 mm, 1.8 μm). Samples (1 μL ...
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bioRxiv - Neuroscience 2022Quote: ... Chromatographic separations were performed on a Zorbax Eclipse Plus C18 RRHD (5 × 2.1 mm, 1.8 μm, Agilent Technologies, USA) column ...
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bioRxiv - Bioengineering 2022Quote: ... RNA concentration was measured for both isolated RNA aliquots using a Cytation 5 Multi-Mode Reader (Agilent Biotek Instruments) with a Take3 Micro-Volume Plate at the wavelengths of 260 ...
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bioRxiv - Microbiology 2022Quote: ... The HNO3 concentration was adjusted to 5% and the zinc concentration was determined by ICP-MS (Agilent7500cx, Agilent Technologies).
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bioRxiv - Physiology 2024Quote: ... Rehydrated 5-μm sections were stained with primary antibodies against insulin (dilution 1:1000; Dako, Santa Clara, CA, USA), somatostatin (dilution 1:300 ...
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bioRxiv - Biochemistry 2024Quote: ... The material was repeatedly injected into an analytical Eclipse XDB-C8 column (Agilent, 5 μm, 4.6 x 150 mm), operating at a flow rate of 0.5 mL/min with a mobile phase of H2O and MeCN (+0.1% TFA) ...
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bioRxiv - Physiology 2024Quote: ... live cells stained with Hoechst 33342 (1:2,000 dilution) (ThermoScientific, Cat#62249) were enumerated using Cytation 5 plate reader (BioTek Instruments - Agilent). Values obtained from the Mito-Stress test were normalized per 1,000 cells.
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bioRxiv - Cell Biology 2024Quote: ... Approximately 120 µg of solubilized membrane fractions were fractionated into 89 fractions using an äkta pure chromatography system (Cytiva) equipped with a Bio-SEC-5 1000Å analytical column (300×7.8 mm, Agilent) and a SEC-5 1000 Å guard column (50×4.6 mm ...
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bioRxiv - Microbiology 2024Quote: ... with a ZORBAX SB-C18 column (particle size, 5 μm; 250 mm × 4.6 mm) (Agilent Technologies, Santa Clara, USA) and a UV diode array detector (Agilent Technologies ...
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bioRxiv - Molecular Biology 2024Quote: ... conducted using an Agilent 1100 Series HPLC and C18 column (Eclipse XDB-C18 5 μm, 4.6×150 mm (Agilent)) ...
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bioRxiv - Cell Biology 2023Quote: ... The α10-N839Q mutation was generated by QuickChange mutagenesis kit following the instruction (cat# 200517-5, Agilent Technologies, Inc.). All DNA constructs were identified by sanger sequencing service provided by Retrogen ...
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bioRxiv - Physiology 2023Quote: ... during which lipids were separated on an Eclipse XDB C18 5-μm column (i.d. 150 × 4.6 mm; Agilent Technologies) using an isocratic mobile phase comprising chloroform:methanol (1:1 v/v ...
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bioRxiv - Physiology 2023Quote: ... The purity of LCA probe was determined to be >95% by HPLC with UV detection at 220 nm (HD-C18 column, 5 μm, 4.6 × 250 mm, Agilent Technologies ...
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bioRxiv - Biochemistry 2023Quote: ... Gly241Glu and Asp235Ala:Gly241Glu were introduced into the previously published vectors (5) using the QuickChange II Site-directed mutagenesis kit (Agilent) using primers listed in Table S2.
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bioRxiv - Biochemistry 2023Quote: ... Colonies were stained with Wright-Giesma and plates were scanned using the Biotek Cytation 5 Imaging Multimodal Reader (Agilent). From scanned images ...
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bioRxiv - Synthetic Biology 2023Quote: ... fitted with a guard column (Zorbax Eclipse XDB-C18, 2.1 x 5 mm 1.8 μm, Agilent part # 821725-903) using a 1290 Infinity II UHPLC (G7120AR ...
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bioRxiv - Bioengineering 2024Quote: ... Primary Cells were stained with DAPI and then imaged with a high throughput imaging system (BioTek Cytation 5, Agilent).
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bioRxiv - Cancer Biology 2023Quote: ... used a two solvent gradient (Mobile Phase A: 10 mM Ammonium Acetate in 90/10 Water/Acetonitrile, pH 9.2, 5 μm Agilent InfinityLab Deactivator Additive ...
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bioRxiv - Plant Biology 2024Quote: ... The organic phase was dried using anhydrous MgSO4 and analysed on GC-2010 Pro gas chromatograph (Shimadzu, Japan) equipped with an HP-5MS 30 m × 0.25 mm (5%-Phenyl)-methylpolysiloxane column (19091S-133, Agilent) with nitrogen as the carrier gas with splitless injection mode ...
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bioRxiv - Immunology 2022Quote: ... The HPLC system consisted of a Thermo Scientific Ultimate 3000 equipped with a UV detector set at 274 nm and a reverse phase Zorbax 300SB-C18 column (4.6 × 250 mm i.d., pore size 5 μm Agilent, U.S.A.). The mobile phase consisted of a 50/50 acetonitrile/buffer (0,02 % ammonium acetate solution at pH 5 ...
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bioRxiv - Cell Biology 2022Quote: ... In brief, peptides were loaded on a Zorbax 300-Extend-C-18 (5 µm, 4.6 × 250 mm) column (Agilent) at a constant flow rate of 1 mL/min ...
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bioRxiv - Microbiology 2022Quote: ... Microarray images were collected by using sure scan microarray scanner system at 5 μm resolution (Agilent Technologies, Scanner 2600D). Fluorescent intensities were quantified using feature extraction software ...
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bioRxiv - Plant Biology 2022Quote: ... and 1 mL injections (passed through silica to filter out precipitate following derivatization) on a Zorbax RX-silica (250 x 4.6 mm, 5 µm; Agilent) column and a mobile phase consisting of solvent A (100% hexanes ...
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bioRxiv - Genomics 2022Quote: ... The GC was equipped with a 30 m DB-35MS + 5 m DuraGuard capillary column (0.25 mm inner diameter, 0.25 μm film thickness; Agilent Technologies). Helium was used as carrier gas at a flow rate of 1.0 mL min−1 and the GC-oven was run at following temperatures and times per sample ...
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bioRxiv - Microbiology 2023Quote: ... of the in vitro SAM cleavage reaction were separated by reverse-phase high performance liquid chromatography (HPLC) with Zorbax Eclipse Plus C18 5-μm (4.6 by 250 mm) column (Agilent) connected to the 1220 Infinity II LC system (Agilent) ...
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bioRxiv - Microbiology 2023Quote: ... Using a LC-10AT HPLC instrument (Shimadzu), the pooled sample was loaded onto a ZORBAX Eclipse XDB C18 column (5 μm, 4.6 × 150 mm) (Agilent) that had been equilibrated with 25 mM sodium acetate ...
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bioRxiv - Cancer Biology 2023Quote: ... for 5 minutes and incubated with the primary antibody of interest which was prepared in antibody diluent (S0809, Dako). Slides were put in a humidified chamber at 4C overnight ...
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bioRxiv - Plant Biology 2024Quote: ... using a HP-5MSI 5% phenyl methyl silox with 30 m × 250 µM × 0.25 µm film thickness (Agilent Technologies). Identification and quantification were conducted using AMDIS (Automated Mass spectral Deconvolution and Identification System ...
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bioRxiv - Physiology 2024Quote: ... and injected 100 µL of sample into an Agilent 1100 series HPLC fitted with a Zorbax RX-SIL column (4.6x25 mm, 5 µm, Agilent). Elution of samples was achieved by a gradient mobile phase of 0.5% ethyl acetate in hexane for 5 minutes then increased to 10% ethyl acetate in hexane for 20 minutes ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... As species were separated by ion-pairing chromatography using a Zorbax SB-C18 column (150 mm × 4.6 mm, 5 μm particle size, Agilent) through isocratic elution for 25 min with the mobile phase used for extraction ...