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Citations for Agilent :
451 - 500 of 1630 citations for 4 1H Benzoimidazol 2 ylsulfanyl butyric acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... The D614G amino acid change was introduced into VRC7480 by site-directed mutagenesis using the QuikChange Lightning Site-Directed Mutagenesis Kit from Agilent Technologies (catalog no ...
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bioRxiv - Immunology 2021Quote: ... we changed three amino acids within the Fc region by using the QuikChange II site directed mutagenesis kits (Agilent, #200523), introducing M257Y ...
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bioRxiv - Neuroscience 2021Quote: ... TDP-43N-Del was generated by deletion of the first 81 amino acids from the N-terminus of TDP-43WT using the QuickChange Site-Directed Mutagenesis Kit (Agilent).
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bioRxiv - Molecular Biology 2020Quote: The conservative lysine at position 23 was replaced with glutamic acid to create Meier-Gorlin mutation (K23E) following site-directed mutagenesis protocol (Agilent). Drosophila-Human and Human-Drosophila hybrids were designed by using a PCR technique ...
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bioRxiv - Plant Biology 2021Quote: Salicylic acid (SA) was extracted from frozen leaf material and analyzed by ultra-high-performance liquid chromatography system (Agilent Technologies) coupled to an Agilent 6530 quadrupole time of flight-mass spectrometer (Agilent Technologies ...
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bioRxiv - Cell Biology 2020Quote: The mCherry-TRAK1 deletion mutant mCherry-TRAK1Δ was obtained by inserting a stop codon after amino acid 635 of the mCherry-TRAK1 encoding nucleotide sequence by means of a PCR-based mutagenesis (Agilent Technologies ...
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bioRxiv - Microbiology 2021Quote: ... The extracted phospholipids were methanolized as fatty-acid methyl esters and then analysed using gas chromatography (Agilent Technologies 6890N, UK) [26] ...
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bioRxiv - Microbiology 2020Quote: ... The GFP-tagged N127143Q mutant was constructed by substitution of asparagines for glutamines at amino acids 127 and 143 by site-directed mutagenesis (Stratagene). The GFP-Rem-T7 partial deletion mutants (GFP-RemΔ103-155-T7 ...
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bioRxiv - Cell Biology 2022Quote: ... Peptides were acidified by 1% trifluoroacetic acid (TFA) and C18 cleanup was performed using Bravo AssayMAP robot with C18 cartridges (Agilent) following the manufacturer’s instruction ...
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bioRxiv - Microbiology 2022Quote: ... The libraries were submitted to the VCU’s Nucleic Acid Sequencing core facility where the quality of the libraries was verified using the bioanalyzer (Agilent Technologies).
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bioRxiv - Physiology 2023Quote: ... Yttrium concentrations were determined in diets and feces after an acid digestion with HNO3 and measured with a coupled plasma-mass spectrometry (Agilent 4200 MP-AES ...
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bioRxiv - Physiology 2022Quote: ... This construct has an artifactual amino acid change in its coding sequence (I143V) and site-directed mutagenesis (Quikchange II XL, Agilent) was performed to convert it back to WT with following two primers (all primers were ordered from Integrated DNA Technologies):
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bioRxiv - Molecular Biology 2022Quote: ... Free fatty acids were separated on a Zorbax StableBond aq column (dimension: 2.1mm x 50mm, particle size: 1.7µm) (Agilent technologies, 827700–914) at 0.6ml/min using a mobile phase solvent consisted of 0.06% acetic acid in water (A ...
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bioRxiv - Neuroscience 2024Quote: ... Point amino acid substitutions in genes encoding GluN subunits were performed using the QuikChange site-directed mutagenesis kit (Agilent Technologies) and verified by DNA sequencing.
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bioRxiv - Biochemistry 2023Quote: ... The resulting peptide mixture was acidified by addition of 1% trifluoroacetic acid (TFA) and desalted on a reversed phase (RP) C18 OMIX tip (Agilent). The tip was first washed 3 times with 200 µl pre-wash buffer (0.1% TFA in water/acetonitrile (ACN ...
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bioRxiv - Immunology 2023Quote: ... stopped with Stop Solution (acid) and absorbance values were recorded at 450 nm on a spectrophotometer (using a BioTeck Gen5 plate reader (Agilent).
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bioRxiv - Microbiology 2023Quote: ... Peptides were acidified with trifluoroacetic acid (TFA) to lower the pH below 3 and desalted on reversed phase (RP) C18 OMIX tips (Agilent). The tips were first washed 3 times with 100 µl pre-wash buffer (0.1% TFA in water/acetonitrile (ACN ...
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bioRxiv - Microbiology 2023Quote: Binding of VHHs to spike proteins on the surface of mammalian cells was determined by flow cytometry using pcG1-expression plasmids containing the coding sequence of the SARS-CoV-2 spike protein from which the C-terminal 18 amino acids were deleted and in which the D614G substitution was introduced by QuickChange site-directed mutagenesis (Agilent) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: The substitution of amino acids via SDM was carried out using the QuikChange II Site-Directed Mutagenesis Kit (Agilent Technologies) per the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: The rate of fatty acids oxidation was determined in 30 days old midbrain organoids following Seahorse XF Mito fuel flex test protocol (Agilent) and using Seahorse XFe96 Spheroid FluxPak (Agilent ...
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bioRxiv - Cell Biology 2024Quote: ... were designed to replace the relevant amino acid residue with alanine and carried out using the PfuUltra II Fusion HS DNA Polymerase kit (600670, Agilent) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: The site-directed mutants of PtPelA and PaPelA without its predicted signal sequence (amino acids 47-948 for PaPelA; accession Q9HZE4) were generated using the QuikChange Lightning site-directed mutagenesis kit (Stratagene). The sequence of all vectors was confirmed using DNA sequencing.
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bioRxiv - Microbiology 2024Quote: ... Plasmids pLB21 and pLB24 were used to further exchange the minD isoleucine 260 to glutamic acid (I260E) by QuickChange site-directed mutagenesis reaction (Stratagene) with oligonucleotide pair HS205/HS206 ...
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bioRxiv - Pathology 2024Quote: Twenty to fifty grams of frozen caecal contents were used to analyzed the short-chain fatty acids (SCFAs) composition using gas chromatography (Agilent 7890B gas chromatograph ...
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bioRxiv - Neuroscience 2024Quote: The fatty acid composition of the plasma samples was analyzed by gas chromatography coupled with a flame ionization detector (model 6890; Agilent). 1 µl of the sample was injected in splitless mode at 250 °C ...
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NR2F2 Reactivation in Early-life Adipocyte Stem-like Cells Rescues Adipocyte Mitochondrial OxidationbioRxiv - Physiology 2024Quote: ... 1µL of pentafluorobenzyl-fatty acid derivatives was injected and data were collected on the GC-MS (8890 GC, 5977B MSD, Agilent) DB-1MS UI column (122-0112UI ...
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bioRxiv - Genetics 2024Quote: ... the reaction was stopped with the addition of 50 µL of sulfuric acid (1N) and the absorbance measured within 15 min at 450 nm using the Biotek Epoch Gen5 spectrophotometer (Agilent). The unknown aldosterone concentrations were extrapolating from the standard curve generated by the Gen5 software (v.2.09) ...
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bioRxiv - Biochemistry 2024Quote: ... a custom Agilent Personal Compound Database and Library (PCDL) of target metabolites (glycolytic and tricarboxylic acid cycle intermediates) was created from Agilent METLIN PCDL ...
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bioRxiv - Cell Biology 2024Quote: ... and bioenergetic profiles were measured with Real-Time ATP Rate Assay and Long Chain Fatty Acid (LCFA) Oxidation Stress Assay (Agilent). Concentrations of the used inhibitors were ...
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bioRxiv - Neuroscience 2022Quote: ... with ODS column (2 x 50 mm, 2 μm) coupled to Agilent LC/MSD TOF MS system (Agilent Technologies Inc, Wadbronn, Germany). For chromatographic separation ...
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bioRxiv - Neuroscience 2022Quote: ... Rat GluN2B-G689C-C1/2 and Rat GluN2B-G689S-C1/2 were generated using the QuikChange Site-Directed Mutagenesis Kit (Agilent,Cat. # 200518). Primers for GluN2B-G689C Mutagenesis ...
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bioRxiv - Systems Biology 2024Quote: ... and detected by 1:2000 dilution of respective secondary HRP-antibody-conjugates (anti-rabbit, P044801-2, Agilent; anti-mouse, P044701-2, Dako).
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bioRxiv - Cancer Biology 2021Quote: Antibodies were diluted in antibody diluent (Dako, S302281-2). Nuclei were visualized by DAPI (Sigma ...
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bioRxiv - Cancer Biology 2021Quote: ... and blocked with Protein-Block reagent (Dako, X090930-2) at room temperature for 30 minutes ...
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bioRxiv - Cancer Biology 2021Quote: ... rinsed three times in wash buffer (Dako, K800721-2), and blocked with 5% bovine serum albumin (BSA ...
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bioRxiv - Cell Biology 2020Quote: ... 2 μl of sample was subjected to TapeStation (Agilent) analysis to ascertain band sizes ...
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bioRxiv - Developmental Biology 2021Quote: ... A rabbit anti-GFAP (Dako # Z033401-2, 1:250) primary antibody was diluted in blocking solution for incubation at 4°C overnight ...
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bioRxiv - Neuroscience 2021Quote: ... Mouse anti-Ki67 (1:400, M724029-2, Agilent, UK) was used to label proliferative cells and rabbit anti-cleaved-Caspase3 (1:40 ...
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bioRxiv - Cancer Biology 2021Quote: ... Ki-67 (M7240) (M724029-2, Agilent, 1:400 dilution), γH2AX (phospho-S139 ...
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bioRxiv - Biochemistry 2020Quote: ... or anti-rabbit-HRP conjugate (Agilent Dako P044801-2) and visualized with Western Blotting Luminol Reagent (Santa Cruz Biotechnology sc-2048 ...
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bioRxiv - Biochemistry 2020Quote: ... or anti-rabbit-HRP conjugate (Agilent Dako P044801-2) and visualized with Western Blotting Luminol Reagent (Santa Cruz Biotechnology sc-2048 ...
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bioRxiv - Cell Biology 2021Quote: ... Coverslips were mounted in Glycergel (Agilent Dako, C056330-2) and observed using a Nikon Eclipse 80i epifluorescence microscope (Nikon Instruments ...
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bioRxiv - Cell Biology 2021Quote: ... Coverslips were mounted in Glycergel (Agilent Dako, C056330-2) and observed using a Nikon Eclipse 80i epifluorescence microscope (Nikon Instruments ...
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bioRxiv - Neuroscience 2020Quote: ... mouse clone CR3/43 (1:20, Agilent, M077501–2). Sections were incubated for 1 hour at room temperature with secondary antibodies (1:1000) ...
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bioRxiv - Microbiology 2021Quote: ... rabbit anti-MPO (1:200, A039829-2, Dako Agilent Pathology Solutions ...
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bioRxiv - Microbiology 2021Quote: ... rabbit anti-CD3 (1:200, A045229-2, Dako Agilent Pathology Solutions ...
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bioRxiv - Cancer Biology 2020Quote: ... 1X EnVision FLEX Wash Buffer (Agilent DAKO, K800721-2) was used to wash slides followed by treatment with Peroxidazed 1 (Biocare Medical ...
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bioRxiv - Cancer Biology 2020Quote: ... 1X EnVision FLEX Wash Buffer (Agilent DAKO, K800721-2) was used to wash slides followed by treatment with Peroxidazed 1 (Biocare Medical ...
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HNRNPA2B1 controls an unfolded protein response-related prognostic gene signature in prostate cancerbioRxiv - Cancer Biology 2022Quote: ... and anti-rabbit IgG HRP-linked (P044801-2, Dako). The IRE1 inhibitor STF083010 was purchased from Merck Life Science ...
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bioRxiv - Cancer Biology 2022Quote: ... anti-Cytokeratin 7 (1:00; Agilent Technologies, M701829-2), anti-Cytokeratin 19 (1:100 ...