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Citations for Agilent :
451 - 500 of 3268 citations for 3 Cyclohex 1 enyl acrylic acid methyl ester since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: FFPE tissue sections were deparaffinized and antigen retrieval was undertaken in 0.1 M citric acid buffer solution (Dako, target retrieval solution, S169984-2) using an electric pressure cooker (Russell Hobbs RHNHP401) ...
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bioRxiv - Physiology 2021Quote: ... Amino acid substitution mutants were generated by site-directed mutagenesis using a QuickChange site-directed mutagenesis kit (Agilent Technologies, La Jolla, CA, USA). The predicted DNA sequences of all constructs were verified by sequencing.
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bioRxiv - Cancer Biology 2023Quote: ... The oxygen consumption rate (OCR) assay was performed using the XF long-chain fatty acid oxidation (LCFA) stress test kit (Agilent, cat# 103672-100) and Seahorse XFe24 Analyzer was used to investigate the long-chain fatty acid OCR according to the manufacturer’s protocol.
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bioRxiv - Bioengineering 2023Quote: ... An aliquot of 1 mL was obtained and diluted with 2% nitric acid before running for elemental analysis using an inductively coupled plasma optical emission spectrometer (ICP-OES, Varian 720-ES, Agilent, Santa Clara, CA). For each run ...
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bioRxiv - Bioengineering 2020Quote: ... The sections were then deparaffinized in xylene and treated for 3 hours with antigen retrieval solution (Dako) at 80°C ...
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bioRxiv - Biochemistry 2020Quote: ... WH1-2 (922μM) and WH3-4 (3691μM) were passed through a Bio SEC-3 HPLC column (Agilent) at 0.2ml/min in 50mM Tris-HCl pH 7.5 ...
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bioRxiv - Neuroscience 2020Quote: ... Tissue was washed 3 times with PBS (10 minutes each) and mounted in DAKO mounting medium (Agilent) containing DAPI.
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Adaptive translational pausing is a hallmark of the cellular response to severe environmental stressbioRxiv - Molecular Biology 2020Quote: ... cells were washed 3 times with warm PBS and fixed on coverslips with mounting reagent (DAKO,S3025). Cell images were taken by confocal microscopy (Fluoview FV100 ...
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bioRxiv - Genetics 2022Quote: ... Slides were scanned using a G2565 scanner at 3-lm resolution (Agilent Technologies, Palo Alto, CA, USA), and array images were analyzed using NimbleScan v2.5 software (Roche NimbleGen) ...
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bioRxiv - Plant Biology 2021Quote: ... Collected volatiles were thermally desorbed for 3 min at 220°C and analyzed by GC-MS (Agilent 7820A GC interfaced with and Agilent 5977E MSD ...
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bioRxiv - Microbiology 2021Quote: ... 50 μl of protein (10 mg/ml) were injected into a size-exclusion column (Agilent BioSec-3) equilibrated in 50 mM Tris ...
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bioRxiv - Biochemistry 2023Quote: ... The compounds were analyzed on a Poroshell 120 EC-C18 (Agilent, 3 x 150 mm, 2.7 µm) reversed phase column using 0.1% formic acid in water (A ...
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bioRxiv - Microbiology 2022Quote: ... Optical density was read at 405 nm on a Cytation 3 Cell Imaging Multi-Mode Reader (Agilent) and analysed in Bioek Gen5 (Agilent).
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bioRxiv - Biochemistry 2024Quote: ... The compounds were analyzed on a Poroshell 120 EC-C18 (Agilent, 3 x 150 mm, 2.7 µm) reversed phase column with 0.1% formic acid in water (A ...
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bioRxiv - Molecular Biology 2023Quote: ... All scattering intensities were collected on the elution peaks after injection on a BioSEC-3 column (Agilent) equilibrated in 20 mM Na-HEPES pH 7.3 ...
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bioRxiv - Biochemistry 2023Quote: ... The compounds were analyzed on a Poroshell 120 EC-C18 (Agilent, 3 x 150 mm, 2.7 µm) reversed phase column using 0.1% formic acid in water (A ...
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bioRxiv - Plant Biology 2023Quote: ... volatiles were thermally desorbed for 3 min at 220 °C and analyzed using GC-MS (Agilent, USA). Helium was used as the carrier gas at a flow-rate of 1 ml min-1 with a temperature gradient of 5 °C/min from 60 °C (1 min hold ...
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bioRxiv - Cancer Biology 2022Quote: ... Chromatographic separation was performed using a reverse phase column (C18 Zorbax, 50 × 3 mm, 1.7 μm, Agilent) and a mobile gradient phase (400 μL/min ...
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bioRxiv - Genomics 2022Quote: Cyanine-3 labeled cRNA was prepared using the One-Color Low input Quick Amp Labeling kit (Agilent). Dye incorporation and cRNA yield was measured with a NanoDrop ND1000 spectrophotometer (Thermofisher) ...
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bioRxiv - Biochemistry 2023Quote: ... The compounds were analyzed on a Poroshell 120 EC-C18 (Agilent, 3 x 150 mm, 2.7 µm) reversed phase column ...
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bioRxiv - Genetics 2024Quote: ... phoenix gag-pol packaging cells were transfected with 3 µg of pVPack-VSV-G (Agilent technologies, UK) along with 3 µg of pMXs.hOct4 (Octamer-Binding Protein 4 ...
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bioRxiv - Microbiology 2021Quote: ... and branched-chain amino acids (BCAAs) was performed with an Agilent 7890A gas chromatograph coupled with an Agilent 5975 mass spectrometer (Agilent Technologies Santa Clara, CA) using a propyl esterification method as previously described [61] ...
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bioRxiv - Immunology 2022Quote: ... Reactions were stopped by adding 50 μL 3M hydrochloric acid and absorbance at 492 nm was determined on a Synergy 4 plate reader (BioTek, Agilent Technologies inc., CA, USA) or similar ...
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bioRxiv - Biochemistry 2021Quote: ... Peptides were loaded with solvent A (0.1% formic acid in water) onto an in-house packed analytical column filled with Poroshell EC120 C18 material (Agilent Technologies, Santa Clara, CA, USA). Peptides were chromatographically separated at a constant flow rate of 250 nL/min using the following gradient ...
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bioRxiv - Physiology 2024Quote: ... Etomoxir (an inhibitor of long chain fatty acid oxidation) provided in the Seahorse XF Mito fuel flex test kit (Agilent Technologies, Cat#103260-100). Fuel dependency for a specific pathway was measured after inhibiting this pathway ...
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bioRxiv - Biochemistry 2022Quote: ... system coupled to an Agilent 6470 series QQQ mass spectrometer (MS/MS) was used to analyze fatty acids in each sample (Agilent Technologies, Santa Clara, CA). The methods are similar to what is described in the paper by Yang ...
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bioRxiv - Genomics 2024Quote: ... The measurement of the mineral nutrients in the acid digests was conducted by inductively coupled plasma optical emission spectroscopy (ICP-OES; Agilent 5110 Vertical Dual View). The measurements were verified by using certified standard reference material (SRM ...
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bioRxiv - Immunology 2024Quote: ... The reaction was stopped with trifluoroacetic acid, and peptides were analyzed by RP-HPLC (Shimatzu, Kyoto, Japan) on an AdvanceBio Peptide Plus C18 column (Agilent Technologies, Santa Clara, CA). The samples (100 µL ...
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bioRxiv - Pathology 2024Quote: Twenty to fifty grams of frozen caecal contents were used to analyzed the short-chain fatty acids (SCFAs) composition using gas chromatography (Agilent 7890B gas chromatograph, Agilent Technologies, Les Ulis, France) as previously described (25).
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bioRxiv - Neuroscience 2022Quote: ... 1 × 1 M (Agilent Technologies). Genomic DNA from wild-type mouse ES cells (CMTI-2 ...
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bioRxiv - Developmental Biology 2021Quote: ... 0.1 x volume of 3M Acetate (pH 5.5) and 3 x volume of 100% ethanol.Library sizes were measured on 2100 Bioanalyzer instrument (Agilent) using the Agilent High Sensitivity DNA kit (Agilent ...
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bioRxiv - Neuroscience 2021Quote: ... Next day membranes were washed for 3 x 5 min and incubated with HRP-coupled secondary antibodies (DAKO) diluted 1:3000 in washing buffer for 1.5 hour at RT ...
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bioRxiv - Cell Biology 2022Quote: ... Cells (3 · 104 hPASMC per well) were seeded onto XF96 V3 PS Cell Culture Microplates (101085-004, Agilent) and exposed to air or CSE for 24 hours before being loaded into the analyzer ...
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bioRxiv - Bioengineering 2021Quote: ... Sections were then incubated for 40 mins in 3% H2O2 TBST solution and blocked with protein block (Dako) for 20 mins ...
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bioRxiv - Cancer Biology 2021Quote: ... The labeled complementary RNAs were hybridized onto a whole human genome oligo microarray (4 3 44K; Agilent Technologies). After washing the slides ...
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bioRxiv - Molecular Biology 2023Quote: ... The 5’mitoUTR was cloned into the TFAM-mScarlet/pWPXL or the 3’mitoUTR_TFAM-mScarlet_pWPXL plasmid using megaprimers following the QuikChange II XL Site-Directed Mutagenesis Kit (Agilent) large insertion protocol provided by the manufacturer ...
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bioRxiv - Neuroscience 2022Quote: ... and 3 μM BPTES (Sigma-Aldrich, SML0601) (all XFp Mito Fuel Flex Test Kit, Agilent Technologies 103270-100) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... followed by another containing 3 μM BPTES (all XFp Mito Fuel Flex Test Kit, Agilent Technologies, 103270-100) according to manufacturer’s instructions.
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bioRxiv - Biochemistry 2024Quote: ... The test compounds were analyzed on a Poroshell 120 EC-C18 (Agilent, 3 x 150 mm, 2.7 µm) reversed phase column with 0.1% formic acid in water (A ...
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bioRxiv - Biochemistry 2024Quote: ... and the filtrate was analyzed on a Poroshell 120 EC-C18 (Agilent, 3 x 150 mm, 2.7 µm) reversed phase column with 0.1% formic acid in water (A ...
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bioRxiv - Microbiology 2022Quote: ... The optical density (OD) was measured at 405nm using Cytation™ 3 Cell Imaging Multi-Mode Reader (Agilent) and data was analysed using BioTek Gen5 software (Agilent) ...
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bioRxiv - Plant Biology 2022Quote: ... a Poroshell 120 SB C18 column (3 × 100 mm, 2.7 μm, 4.6 × 100 mm, Agilent, Palo Alto, USA) was utilized during the analysis ...
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bioRxiv - Genetics 2023Quote: ... slides were carefully rinsed 3 × 5 min with PBS and slides were mounted with Fluorescent Mounting Medium (Dako) and examined under fluorescence using a Zeiss microscope equipped with an AxioCam HRm camera.
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bioRxiv - Neuroscience 2023Quote: ... Sections were then washed 3 times with PBS and mounted using fluorescent mounting medium for imaging (Dako, #S3023).
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bioRxiv - Immunology 2024Quote: ... then in 3% hydrogen peroxide for 15min and in serum-free protein block solution (Dako, Cat # X090930-2) for 30min ...
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bioRxiv - Developmental Biology 2021Quote: ... Sections were then incubated with primary antibodies for 48 h at 4°C (AXIN2 1:100, FZD1 1:100, LEF1 1:100, AQP1 1:100, OTX2 1:50, β-Catenin cell signaling 1:400, β-Catenin Dako 1:200), washed and subsequently incubated with secondary anti-mouse or anti-rabbit antibodies diluted in PBS (at 1:1000 ...
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bioRxiv - Microbiology 2024Quote: ... Two amino acid substitutions (S299A and T309A) were carried out using specific primers (Table S1) and Pfu Turbo Polymerase (Agilent Technologies, Santa Clara, CA, USA). Then amplicons were further treated with DpnI (New England BioLabs ...
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bioRxiv - Pathology 2023Quote: ... SMMS-1 (M3558, Dako, 1:100) and PG-M1 (M0876 ...
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bioRxiv - Cancer Biology 2020Quote: ... analyte separation was achieved using a Synergi Hydro-RP column (2.5 µm 3×100mm, Phenomenax) at ambient temperature on a 1260 Infinity (Agilent)-QTRAP500 (AB Sciex ...
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bioRxiv - Genomics 2022Quote: ... RT was performed with a specific primer (5′-CCTACACGACGCTCTTCC-3′) using AffinityScript Multiple Temperature cDNA Synthesis Kit (Agilent Technologies). RNA degradation was performed by incubating the RT mixture with 10% 1 M NaOH (2μl of RT mixture ...