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Citations for Calbiochem :
101 - 113 of 113 citations for hsa mir 150 3p RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2019Quote: ... 1 mM EDTA, 150 mM NaCl, 1% Triton X-100, 1 mM PMSF and 1X protease inhibitor cocktail III [Calbiochem]). After a 5-minute incubation on ice ...
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bioRxiv - Neuroscience 2023Quote: ... mg of tissue/µl of NP-40 lysis buffer (20mM Tris- HCl pH 7.4, 150 mM NaCl, 100mM EDTA pH 7.4, 1% NP-40; Calbiochem Cat#492016) with protease inhibitors (cOmplete™ ...
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bioRxiv - Cell Biology 2021Quote: ... pre-equilibrated with running buffer (PBS, 20mM imidazole, 10% glycerol, protease Inhibitor Cocktail Set III Calbiochem, Ref. 539134-1 at 1:10,000 dilution). The column was washed with five column volumes (CV ...
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bioRxiv - Molecular Biology 2024Quote: ... cells were released from the arrest by transferring them to α-factor-free medium containing 150 μg/mL of Pronase (Calbiochem) and cultured for 30 min ...
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bioRxiv - Microbiology 2021Quote: ... to OD600 1 were incubated with 750 μl of lysis buffer (100 mM EDTA [pH 8.0], 1 mM MgCl2, 5 μg/ml lysostaphin, 10 μg/ml DNase, proteinase inhibitor [cocktail set III from Calbiochem] diluted 1:100 in PBS) for 30 min at 37°C and homogenized three times with 500 μl zirconia-silica beads (0.1 mm diameter from Carl-Roth ...
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bioRxiv - Neuroscience 2019Quote: ... diluted in KPBS containing 0.3 % Triton X-100 and incubated overnight at RT in cFos rabbit polyclonal antiserum (1:20,000; Calbiochem) diluted in KPBS containing 1 % NGS and 0.3 % Triton X-100 ...
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bioRxiv - Neuroscience 2021Quote: ... the brain was dissected and homogenized in RIPA extraction buffer (10mM HEPES/NaOH pH 7.4, 150mM NaCl, 1% Triton X-100, 1% SDS) with protease inhibitors (Calbiochem Protease Inhibitor Set V EDTA-free). Western blot analysis was performed with Bio-Rad wet transfer system and PVDF membrane (Millipore) ...
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bioRxiv - Neuroscience 2020Quote: ... Bacteria were resuspended in (50mM HEPES, 250mM NaCl, 10% glycerol plus 1% Triton X-100, supplemented with protease inhibitors (Merck, Calbiochem set III, Cat. No. 539134) and subsequently sonicated ...
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bioRxiv - Neuroscience 2020Quote: ... Cell lysis of all samples was performed after two washes with ice-cold PBS by incubating cells for 20 min in ice-cold 1% Triton X100 in PBS with protease inhibitor cocktails (Merck, Calbiochem set III, Cat. No. 539134), subsequent thorough scraping and sonication.
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bioRxiv - Cell Biology 2022Quote: ... or kinase domain ERK3 in kinase buffer (250 mM HEPES pH 7.5, 500 mM NaCl, 25 mM MgCl2, supplemented with protease inhibitor cocktail Set I-Calbiochem 1:100 (Cat# 539131, Merck Millipore) and phosphatase inhibitors ...
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bioRxiv - Neuroscience 2021Quote: ... cells were rinsed two times with pre-warmed medium and incubated for 30 min at 37°C with rabbit anti-GluA1 (1/150; Calbiochem/Millipore, RRID: AB_564636) in culture medium ...
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bioRxiv - Immunology 2023Quote: ... 2 µl were used as PCR template for amplification with the KOD Polymerase (Calbiochem, 71086-3). The PCR products were cloned into the pI.18 vector ...
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bioRxiv - Bioengineering 2020Quote: ... Tested additives were added to the qRT-PCR reaction mix at the following concentrations: 1% w/v Tween-20 (Calbiochem 655204-100mL), 0.8 µg/µL BSA (Fisher BP675-1) ...