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Citations for Calbiochem :
51 - 65 of 65 citations for Water Nuclease Free since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... Culture was then cooled to 16℃ and induced with 0.5 mM dioxane-free isopropyl B-D-1-thiogalactopyranoside (IPTG) (Calbiochem) overnight at 16℃ ...
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bioRxiv - Biochemistry 2023Quote: ... Ubiquitin was expressed as described above except that the 2 L culture was induced with 0.4 mM dioxane-free isopropyl B-D-1-thiogalactopyranoside (IPTG) (Calbiochem) for four hrs at 37℃ ...
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bioRxiv - Biochemistry 2023Quote: ... Culture was then cooled to 16℃ and induced with 0.4 mM dioxane-free isopropyl B-D-1-thiogalactopyranoside (IPTG) (Calbiochem) overnight at 16℃ ...
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bioRxiv - Biochemistry 2023Quote: ... Culture was then cooled to 16℃ and induced with 0.4 mM dioxane-free isopropyl B-D-1-thiogalactopyranoside (IPTG) (Calbiochem) overnight at 16℃ ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... and 0.2% soybean phosphatidylcholine with 100 μl of protease inhibitor cocktail (Protease Inhibitor Cocktail Set III, EDTA-Free, Calbiochem). After stirring for 1 hour at 4°C ...
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bioRxiv - Neuroscience 2019Quote: ... or both hippocampi from a single mouse was homogenized in 2 mL of ice-cold homogenization buffer (0.32 M sucrose, 4 mM HEPES pH 7.4, protease inhibitor cocktail EGTA-free (Calbiochem, 1:1000), RNasin (Promega ...
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bioRxiv - Immunology 2019Quote: Eosinophil degranulation was assessed by performing ELISA for Eosinophil Derived Neurotoxin (EDN) on cell free supernatants from eosinophils cultured for 4hrs with N-Formylmethionyl-leucyl-phenylalanine (FMLP) (Calbiochem) or cytokines ...
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bioRxiv - Systems Biology 2020Quote: ... cell lysis buffer (1X phosphate buffered saline (PBS) with 1% TrintonX-100 and 1% EDTA-free protease inhibitor (Calbiochem, #539134)) was added to each well and the plate was put on ice for 30 minutes ...
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bioRxiv - Biochemistry 2022Quote: ... lysed in M-PER mammalian protein extraction reagent (Thermo-Fisher) with 1/1000 of the EDTA-free protease inhibitor cocktail Set III (Calbiochem), and centrifuged for 30 min at 15,000 × g at 4°C ...
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bioRxiv - Molecular Biology 2022Quote: ... After 4 h the cells were starved in serum free medium overnight and then incubated for 1h with the ADAM17 inhibitor Batimastat (BB-94) at 10 μM (196440, Calbiochem), with the selective ADAM10 inhibitor GI254023X (GI ...
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bioRxiv - Microbiology 2022Quote: ... the preformed spheroids were transferred into the fibrin gel consisting of plasminogen-free human fibrinogen (final concentration 3 mg/ml; Calbiochem, USA) and human thrombin (final concentration 2 U/ml ...
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bioRxiv - Molecular Biology 2024Quote: ... cells were released from the arrest by transferring them to α-factor-free medium containing 150 μg/mL of Pronase (Calbiochem) and cultured for 30 min ...
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bioRxiv - Neuroscience 2019Quote: Pre-sorted synaptosomes (~750 μl) on iced were mixed with ice cold PBS containing protease inhibitor cocktail EGTA-free (Calbiochem, 1:1000) and RNasin (Promega ...
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bioRxiv - Immunology 2020Quote: ... The filtrate was collected in 1.5ml microtube followed by addition of protease inhibitors (Protease Inhibitor Cocktail SetIII, EDTA-free; Calbiochem, San Diego, CA) to protect proteins from endogenous protease activities.
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bioRxiv - Neuroscience 2021Quote: ... the brain was dissected and homogenized in RIPA extraction buffer (10mM HEPES/NaOH pH 7.4, 150mM NaCl, 1% Triton X-100, 1% SDS) with protease inhibitors (Calbiochem Protease Inhibitor Set V EDTA-free). Western blot analysis was performed with Bio-Rad wet transfer system and PVDF membrane (Millipore) ...