Labshake search
Citations for Calbiochem :
151 - 200 of 300 citations for Thioredoxin Domain Containing Protein 3 TXNDC3 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2020Quote: ... Total mouse hippocampus from the left hemisphere was homogenised using a mechanical homogeniser and disposable pestles (Anachem) in 100μl TBS (50mM Tris-HCl pH 8.0) containing protease and phosphatase inhibitors (Calbiochem). The homogenate was then centrifuged at 4 °C for 30 mins at 16,000 × g ...
-
bioRxiv - Neuroscience 2019Quote: ... diluted in KPBS containing 0.3 % Triton X-100 and incubated overnight at RT in cFos rabbit polyclonal antiserum (1:20,000; Calbiochem) diluted in KPBS containing 1 % NGS and 0.3 % Triton X-100 ...
-
bioRxiv - Biochemistry 2021Quote: ... Cell pellets were sonicated in 20 mM HEPES buffer pH 7.5 containing 0.25 M sucrose and 1:100 protease inhibitor cocktail set V (Calbiochem) and centrifuged at 2,000 × g for 10 min to pellet nuclear fraction ...
-
bioRxiv - Neuroscience 2023Quote: ... we used a pipette solution containing 50-100 ng/ml amphotericin B as permeabilizing agent (Calbiochem, Madrid, Spain) and a pipette-filling solution containing (in mM) ...
-
bioRxiv - Cell Biology 2023Quote: ... mouse organs were homogenized in 2% SDS lysis buffer containing protease inhibitor mixture III (# 535140, Calbiochem, Billerica, MA) and phosphatase inhibitors (#P2850 ...
-
bioRxiv - Pharmacology and Toxicology 2024Quote: ... collected pRPE cells were suspended in a hypotonic buffer containing protease inhibitor cocktail (MilliporeSigma™ Calbiochem™, 53913110VL). The pRPE cells underwent disruption with a Dounce tissue homogenizer ...
-
bioRxiv - Cell Biology 2020Quote: ... Primary antibodies were: GAPDH (clone 6C5, #CB1001, Calbiochem), p16A (ArpC5A ...
-
bioRxiv - Cancer Biology 2022Quote: ... PAX3 (Rabbit Polyclonal Antibody Cat. No. CA1010, Calbiochem), GLI1 (Rabbit Polyclonal Antibody ...
-
bioRxiv - Molecular Biology 2020Quote: ... 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reagent was obtained from Calbiochem (San Diego, CA, USA).
-
bioRxiv - Neuroscience 2022Quote: ... the cells were then incubated overnight at 4°C with primary antibodies (rabbit anti-AUTS2, 1:200, Atlas Antibodies; rabbit anti-GRIA1, 1:10, Calbiochem; mouse anti-GRIA2 ...
-
bioRxiv - Molecular Biology 2020Quote: ... Plates were then washed to remove free protein prior to addition of nitrocefin (Calbiochem) for 1 hour at room temperature ...
-
bioRxiv - Cancer Biology 2021Quote: Cell pellets were sonicated in ice-cold lysis buffer (containing 20 mM Tris-HCl, pH 7.4, 5 mM EDTA, 10 mM Na4P2O7 (Calbiochem), 100 mM NaF ...
-
bioRxiv - Cancer Biology 2021Quote: Cell cultures in 10cm dishes were scrape-harvested in 500 μL lysis buffer containing 0.2% (v/v) protease inhibitors (Protease Inhibitor Cocktail set III, Calbiochem). Lysis buffer comprised ...
-
bioRxiv - Immunology 2020Quote: ... Fixed cells were washed three times in PBS and permeabilized by incubating them in PBS containing 5% non-fat milk and 0.05% saponin (Calbiochem). Coverslips were placed in primary antibody for 1 hour then washed 3x in PBS and placed in secondary antibody ...
-
bioRxiv - Neuroscience 2020Quote: ... A half cortex was weighed on a microscale and homogenised in 4 volumes of ice-cold Tris-buffered saline (TBS) (50mM Tris-HCl pH 8.0) containing a cocktail of protease and phosphatase inhibitors (Calbiochem) using a handheld mechanical homogeniser and disposable pestles (Anachem) ...
-
bioRxiv - Neuroscience 2020Quote: ... in approximately 5 volumes of ice-cold Tris-buffered saline (TBS) (50 mM Tris-HCL, pH 8.0) containing a cocktail of protease and phosphatase inhibitors (Calbiochem). Homogenates were centrifuged at 16,000 x g at 4 °C for 30 min ...
-
bioRxiv - Neuroscience 2022Quote: ... A half cortex was weighed on a microscale and homogenised in 4 volumes of ice-cold Tris-buffered saline (TBS) (50mM Tris-HCl pH 8.0) containing a cocktail of protease and phosphatase inhibitors (Calbiochem) using a handheld mechanical homogeniser and disposable pestles (Anachem) ...
-
bioRxiv - Neuroscience 2022Quote: A half cortex was weighed on a microscale and homogenised in 3 volumes of ice-cold Tris-buffered saline (TBS) (50mM Tris-HCl pH 8.0) containing a cocktail of protease and phosphatase inhibitors (Calbiochem) using a handheld mechanical homogeniser and disposable pestles (Anachem ...
-
bioRxiv - Biochemistry 2021Quote: ... A custom compound library containing over 5000 compounds assembled from commercial sources (Sigma, Selleck, Enzo, Tocris, Calbiochem, and Symansis) were distributed in 24 plates using an Echo 550 (Labcyte ...
-
bioRxiv - Cell Biology 2023Quote: ... cells were washed 3 times for 8 min in PBS-Triton 0.01% with rocking in the dark and glass coverslips were mounted on glass slides using Mowiol containing 0.75 μg/ml DAPI (Calbiochem) and imaged with a 100x or 63x objective using Zeiss epifluorescence microscope ...
-
bioRxiv - Neuroscience 2023Quote: ... Heads were snap-frozen in liquid nitrogen then homogenized using a mechanical homogenizer in 100 μL TBS (50mM Tris-HCl, pH 8.0) containing 1:200 protease inhibitor cocktail set III (Calbiochem). Homogenates were centrifuged at 16,000 g at 4°C for 30 min ...
-
bioRxiv - Cell Biology 2022Quote: ... and at 48 hpi cells transduced with Wasabi-pIPFCW2 were selected with 3-4 mg/ml puromycin (Calbiochem, 540411) and sorted for mid-range expression of Wasabi.
-
bioRxiv - Neuroscience 2022Quote: ... 1.33 mM Ca2+ and 1 mM of the cAMP phosphodiesterase inhibitor 3-isobutyl-1-methylxanthine (IBMX: Calbiochem, Damstard, Germany) were added to the synaptosomes for 15 min ...
-
bioRxiv - Microbiology 2019Quote: ... The blots were washed with PBS and incubated with primary antibodies (anti-EBV gH/gL (a rabbit polyclonal antiserum;1:200) (45) and anti-His tag antibody (OB05, Calbiochem, 1:1000) for His-tagged KSHV gL ...
-
bioRxiv - Plant Biology 2020Quote: ... secondary antibody: anti-rabbit HRP (1:10,000, Calbiochem #401353)).
-
bioRxiv - Cell Biology 2021Quote: ... and GAPDH antibody (CB1001, Calbiochem, mouse monoclonal, 1:5000). For Western blots of RlucC-ER and Mito-RlucN ...
-
bioRxiv - Cancer Biology 2019Quote: ... mouse monoclonal anti-BRCA1 antibody (OP92, Ab-1; Calbiochem, for IPs 10μg/ml ...
-
bioRxiv - Molecular Biology 2020Quote: ... Antibodies against α-tubulin were purchased from Calbiochem (CP06), GFP and for NDUFS3 from Abcam (ab6556 and ab14711 respectively) ...
-
bioRxiv - Microbiology 2019Quote: ... GAPDH was detected with mouse monoclonal antibody 6C5 (Calbiochem). SAG1 levels were used to control for the levels of parasites within the infected cells and blots were stained with polyclonal rabbit anti-SAG1 followed by a secondary goat anti-Rabbit antibody conjugated to HRP ...
-
bioRxiv - Neuroscience 2022Quote: ... mouse anti-CC1 antibody (1:500, Calbiochem, cat# OP80), mouse anti MBP Antibody (1:1000 ...
-
bioRxiv - Cell Biology 2023Quote: ... a neutralizing antibody against E-cadherin (SHE78-7, Calbiochem) was used at the final concentration of either 1 or 3 µm/ml ...
-
bioRxiv - Cell Biology 2020Quote: ... Wnt-surrogate55 (0.15 nM, U-Protein Expression) and Rho kinase inhibitor (10 μM, Calbiochem Y27632) was added to the media whenever cells were at a single-cell stage to help recovery ...
-
bioRxiv - Genetics 2021Quote: ... The larvae were individualized in each well of 96-well plates containing ATR Inhibitor IV (CAS 1232410-49-10 Calbiochem) at 10 µM or DMSO in egg water as negative control ...
-
bioRxiv - Physiology 2019Quote: ... Tissues were homogenized in 20 mM HEPES buffer (pH 7.5) containing 0.25M sucrose and a cocktail of protease inhibitors (Calbiochem, Burlington, MA) followed by ultracentrifugation at 100,000g for 45 min ...
-
bioRxiv - Molecular Biology 2019Quote: ... Transformants were regenerated and selected on regeneration medium containing 100 µg/ml of hygromycin B (Calbiochem, La Jolla, CA, USA) and 150 µg/ml G418 sulfate (Cellgro ...
-
bioRxiv - Systems Biology 2022Quote: ... respectively) were resuspended in 1 mL of ChIP buffer containing 5 μl of protease inhibitor cocktail (CalbioChem, cat # 539134-1) (50 mM Tris-HCl pH 8 ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... containing 100 µg streptomycin ml−1 and 100 U penicillin ml−1 using neomycin (400 µg/ml) (Calbiochem, EMD Chemicals) as a selection marker ...
-
bioRxiv - Microbiology 2019Quote: ... Transformants were regenerated and selected on regeneration medium containing 100 µg/ml of hygromycin B (Calbiochem, La Jolla, CA, USA) and/or 150 µg/ml G418 sulfate (Cellgro ...
-
bioRxiv - Cell Biology 2021Quote: ... Strains expressing both CDC11A-mCherry-NAT and CDC11B-GFP-NEO were constructed by transforming AG230 with a 7678bp fragment from PciI/NotI/PspXI digested AGB1320 and selecting on media containing 100ug/ml G418 (CalBiochem). Transformants were verified by PCR ...
-
bioRxiv - Genomics 2019Quote: ... Pulverized material was thawed in glycerol containing nuclear isolation buffer to stabilize nuclear structure and then filtered through Miracloth (Calbiochem) to remove large tissue debris ...
-
bioRxiv - Cell Biology 2022Quote: ... Forty-eight hours post transfection cells were removed and incubated in 250 μL/well of HEK Lysis Buffer containing protease Inhibitor Cocktail Set V (Calbiochem) before being centrifuged at 13 000 rpm for 10 min ...
-
bioRxiv - Cell Biology 2023Quote: ... cells were washed 3 times with PBS-T and glass coverslips were added on cells (mitosis) or coverslips were mounted onto slides with Mowiol containing DAPI (Calbiochem).
-
bioRxiv - Molecular Biology 2024Quote: ... the transfected cells were split and plated into three 10-cm dishes in culture medium containing selection antibiotics blasticidin S (15 μg/ml, Calbiochem) and hygromycin B (100 μg/ml ...
-
bioRxiv - Immunology 2019Quote: ... Sections were then incubated overnight in (4°C) with anti-c-ErbB2/c-Neu (Ab-3) mouse mAb (3B5) (1:500, Calbiochem). After washing with PBS ...
-
bioRxiv - Immunology 2019Quote: ... bovine serum albumin in 0.1 M phosphate buffer for 1 h at room temperature and incubated overnight (4°C) with anti-c-ErbB2/c-Neu (Ab-3) mouse mAb (3B5) (1:500, Calbiochem) or anti-CD3 (1:500 ...
-
bioRxiv - Cancer Biology 2022Quote: ... cells were washed 3 times with PBS and fixed to a slide using 5 μL of Mowiol (4-88 Reagent, Calbiochem). Fluorescent images were acquired with a Leica Dmi8 microscope (Leica Microsystems).
-
bioRxiv - Cell Biology 2023Quote: ... the cells were fixed with cold methanol (10 min at −20°C) and blocked for 30 min with 3% (w/v) bovine serum albumin (Calbiochem) in 1 x PBS at room temperature ...
-
bioRxiv - Pathology 2024Quote: ... NETs formation was induced for 3 hours at 37°C and 5% CO2 by stimulation with 10 µM of PAF (Calbiochem) on primed neutrophils and 25 µM on non-primed ones ...
-
bioRxiv - Neuroscience 2024Quote: ... The NPCs were differentiated into neurons in the absence of bFGF for at least 3 weeks and treated with 10 μM ROCK inhibitor (Y-27632; Calbiochem) during the first 24 h.
-
bioRxiv - Neuroscience 2020Quote: ... rabbit polyclonal antibody against c-Fos (1:500; PC38T, Calbiochem). Secondary antibodies were anti-isotypic Alexa Fluor conjugates (1:500 ...