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Citations for Calbiochem :
451 - 478 of 478 citations for TIM 1 Mouse HEK 293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... lysed in M-PER mammalian protein extraction reagent (Thermo-Fisher) with 1/1000 of the EDTA-free protease inhibitor cocktail Set III (Calbiochem), and centrifuged for 30 min at 15,000 × g at 4°C ...
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bioRxiv - Immunology 2022Quote: ... Cells were left unstimulated or stimulated for indicated time at 37 °C with PMA (10 ng/ml; Alexis) and ionomycin (1 μM; Calbiochem) or solvent (DMSO) ...
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bioRxiv - Immunology 2022Quote: Caspase-1 activity was determined with the fluorometric substrate Z-YVAD 7-Amido-4-trifluoromethylcoumarin (Z-YVAD-AFC, caspase-1 substrate VI, Calbiochem) as described previously [47,48] ...
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bioRxiv - Biochemistry 2022Quote: ... Nuclei were stained with DAPI (1:1000 in PBS, AppliChem, Darmstadt, Germany) and coverslips were mounted on Mowiol 4-88 (Calbiochem, Merck Millipore ...
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bioRxiv - Neuroscience 2023Quote: ... mg of tissue/µl of NP-40 lysis buffer (20mM Tris- HCl pH 7.4, 150 mM NaCl, 100mM EDTA pH 7.4, 1% NP-40; Calbiochem Cat#492016) with protease inhibitors (cOmplete™ ...
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bioRxiv - Cell Biology 2023Quote: ... and cells were held for 16 hours in 1% Lipoprotein Deficient Serum (LDS) (Biomedical Technologies, BT907) and 25 μg/ml ALLN (Calbiochem) for 30 min prior to harvesting.
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bioRxiv - Cell Biology 2019Quote: ... 2-3 × 105 single cells EpiSCs were seeded on 5 μg/10 cm2 fibronectin-coated 6-well plates in media containing (1 μl/ml) ROCK inhibitor (InSolution™ Y-276320, Cat.#688001-500UG, VWR Calbiochem). In the morning of transfection ...
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bioRxiv - Cell Biology 2021Quote: Commercially available antibodies against the following proteins were used for Western blotting in the indicated dilutions: BRCA2 (OP95, Calbiochem, 1:1000), RAD51 (70-001 ...
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bioRxiv - Neuroscience 2022Quote: ... the slices were incubated overnight in a mixture of primary antibodies anti-FOS (rabbit anti-FOS, 1:10000, cat: p38, Calbiochem, USA) and anti-GAD67 (mouse anti-GAD67 ...
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bioRxiv - Bioengineering 2022Quote: ... All the above stages were performed in cold phosphate-buffered saline (PBS) with 1 × Protease Inhibitor cocktail set I (Calbiochem 539,131). Eyes that were initially fixed in 10% NBF or 2.5% PFA were dissected to create eyecups ...
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bioRxiv - Immunology 2022Quote: ... at a 4:1 molar ratio of monomer:streptavidin in the presence of a 1x protease inhibitor cocktail solution (Calbiochem, Millipore Sigma). 1/5th volumes of streptavidin-PE or streptavidin-BV421 were added to each monomer every 20 minutes and incubated ...
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bioRxiv - Immunology 2023Quote: ... at a 4:1 molar ratio of monomer:streptavidin in the presence of a 1x protease inhibitor cocktail solution (Calbiochem, Millipore Sigma). Briefly ...
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bioRxiv - Molecular Biology 2023Quote: ... cultures were lysed with a solution containing SYBR green (1:5000, Invitrogen Cat. No. S7567) (Lysis buffer: 0.16% saponin (Calbiochem/EMD #558255), 0.05 M TRIS-HCl (Roche Cat ...
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bioRxiv - Molecular Biology 2023Quote: ... 0.1 mM EGTA, 1% Triton X-100, 0.1% sodium deoxycholate, 0.5% SLS, 0.1% SDS, 0.5 mM PMSF, Calbiochem Complete Protease Inhibitor Cocktail V). The suspension was incubated for 30 min at 4°C and then sonicated with Covaris ME220 focused-ultrasonicator (50 alternating 15-s ON and 45-s OFF intervals ...
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bioRxiv - Physiology 2023Quote: ... MLEC were incubated with appropriate fluorophore-labelled secondary antibodies for 1 hour and mounted with Mowiol (Calbiochem, San Diego, CA, USA). Nuclei were visualized by DAPI staining ...
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bioRxiv - Neuroscience 2019Quote: Pre-sorted synaptosomes (~750 μl) on iced were mixed with ice cold PBS containing protease inhibitor cocktail EGTA-free (Calbiochem, 1:1000) and RNasin (Promega ...
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bioRxiv - Bioengineering 2020Quote: ... Tested additives were added to the qRT-PCR reaction mix at the following concentrations: 1% w/v Tween-20 (Calbiochem 655204-100mL), 0.8 µg/µL BSA (Fisher BP675-1) ...
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bioRxiv - Cancer Biology 2020Quote: ... The 2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one (PD98059, MEK inhibitor) and STAT3 inhibitor VI were from Calbiochem (Darmstadt, Germany). The EGF was from Millipore (Billerica ...
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Myosin VI regulates ciliogenesis by promoting the turnover of the centrosomal/satellite protein OFD1bioRxiv - Cell Biology 2021Quote: ... pH 7.5, 250 mM NaCl, 1 mM EDTA, 5% Glycerol, 0.1% NP40, Protease Inhibitor Cocktail set III, Calbiochem, and 0.1 mM PMSF). Sonicated lysates were cleared by centrifugation at 16,000 rpm for 30 minutes ...
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bioRxiv - Physiology 2023Quote: ... Normal goat serum was used as a blocking agent and slides were incubated in biotinylated hyaluronan binding protein (HABP, 1:500, CalBiochem, Burlington, MA) for one hour ...
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bioRxiv - Neuroscience 2021Quote: ... cells were rinsed two times with pre-warmed medium and incubated for 30 min at 37°C with rabbit anti-GluA1 (1/150; Calbiochem/Millipore, RRID: AB_564636) in culture medium ...
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bioRxiv - Cell Biology 2021Quote: ... NaCl-HEPES buffer with antibodies but without uEV sample as well as stained sample supplemented with 1% NP40 (Calbiochem, San Diego, CA, USA) were analyzed as controls ...
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bioRxiv - Cell Biology 2023Quote: ... RPE-1 cells transfected with either siR-NAs targeting Luciferase or MiniBAR for 72 h were treated with Y27632 (Calbiochem 146986-50-7) at 50 µM for 24 h in DMEM-F12 without SVF before fixation.
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bioRxiv - Cancer Biology 2023Quote: ... hatched zebrafish embryos were engrafted via the Duct of Cuvier with 1×105 cells/µL tumor cells resuspended in 2% polyvinypyrrolidone 40 (PVP-40, Calbiochem, San Diego, USA) using borosilicate glass capillary needles (1mm O.D ...
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bioRxiv - Molecular Biology 2023Quote: ... the mice were anaesthetized using 2% isoflurane and given a retro-orbital injection of 21.75 mg kg−1 body weight puromycin (Calbiochem, San Diego, CA, USA) in saline to measure muscle protein synthesis73.
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bioRxiv - Neuroscience 2020Quote: ... Bacteria were resuspended in (50mM HEPES, 250mM NaCl, 10% glycerol plus 1% Triton X-100, supplemented with protease inhibitors (Merck, Calbiochem set III, Cat. No. 539134) and subsequently sonicated ...
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bioRxiv - Neuroscience 2020Quote: ... Cell lysis of all samples was performed after two washes with ice-cold PBS by incubating cells for 20 min in ice-cold 1% Triton X100 in PBS with protease inhibitor cocktails (Merck, Calbiochem set III, Cat. No. 539134), subsequent thorough scraping and sonication.
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bioRxiv - Cell Biology 2022Quote: ... or kinase domain ERK3 in kinase buffer (250 mM HEPES pH 7.5, 500 mM NaCl, 25 mM MgCl2, supplemented with protease inhibitor cocktail Set I-Calbiochem 1:100 (Cat# 539131, Merck Millipore) and phosphatase inhibitors ...