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Citations for Calbiochem :
1 - 50 of 65 citations for Recombinant Red Fluorescent Protein His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... 50 μL mouse monoclonal anti-6X His tagged primary antibody (Calbiochem) was added at a dilution of 1:150 in 0.5 % BSA in PBST in the respective wells and incubated for 2 hours without shaking at room temperature ...
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bioRxiv - Cell Biology 2019Quote: ... and recombinant mouse M-CSF (Calbiochem) at a final concentration of 10ng/mL for seven days ...
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bioRxiv - Cancer Biology 2021Quote: ... recombinant MMP2 was purchased from Calbiochem. DMSO was obtained from Fisher Scientific.
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bioRxiv - Neuroscience 2023Quote: Twenty ng of recombinant proBDNF (Alomone Labs) was incubated with 50 ng of recombinant MMP-9 (Calbiochem) or 50 ng of recombinant ...
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bioRxiv - Physiology 2020Quote: ... Fluorescent samples were mounted with Fluorsave (Calbiochem) and analyzed with an Olympus FV10 confocal microscope.
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bioRxiv - Cell Biology 2021Quote: ... conjugated to Texas Red (Calbiochem, 401355) or alkaline phosphatase (Sigma ...
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bioRxiv - Neuroscience 2019Quote: ... Active recombinant human MMP-9 (Calbiochem, San Diego, CA) was used as a positive control ...
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bioRxiv - Developmental Biology 2023Quote: ... larvae were mounted on slides in Fluorescent Mounting Media (Calbiochem) and imaged within 3 days of processing to prevent signal loss ...
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bioRxiv - Molecular Biology 2020Quote: ... Proteasome activity was quantified using the AMC-tagged proteasome fluorogenic substrate (Succ-LLVY-AMC, Calbiochem), similarly to (33) ...
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bioRxiv - Cell Biology 2019Quote: ... and Scott 2007) stably expressing ARL13B C-terminally tagged with GFP were generated by lentiviral infection followed by puromycin (Calbiochem) selection.
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bioRxiv - Immunology 2021Quote: ... Proteins were precipitated with the ProteoExtract Protein Precipitation Kit (Calbiochem) and quantified by Coomassie (Bradford ...
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bioRxiv - Molecular Biology 2022Quote: ... After adding Protein G Plus Protein A agarose beads (Calbiochem, Germany), the mixture was incubated at 4°C for 1 hour ...
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bioRxiv - Neuroscience 2021Quote: ... Injections of 50 µl NGF at a concentration of 0.8 µM (NGF, human recombinant, Calbiochem, Merck, Germany) dissolved in phosphate buffer saline (PBS ...
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bioRxiv - Animal Behavior and Cognition 2019Quote: ... Immune complexes were captured with Protein G/Protein A plus-agarose (Calbiochem) and subjected to Western blotting using PKL antibody (25 ng/ml ...
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bioRxiv - Systems Biology 2021Quote: ... The proteins were then precipitated using Proteoextract protein precipitation kit (Calbiochem; cat # 539180) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... The blots were washed with PBS and incubated with primary antibodies (anti-EBV gH/gL (a rabbit polyclonal antiserum;1:200) (45) and anti-His tag antibody (OB05, Calbiochem, 1:1000) for His-tagged KSHV gL ...
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bioRxiv - Neuroscience 2019Quote: ... and Protein G beads (CalBiochem). The beads containing Fyn were resuspended in 100 µL buffer (0.1% Triton ...
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bioRxiv - Cell Biology 2020Quote: ... Protein A agarose beads (Calbiochem) was added to the mixture and incubation was continued with gentle rocking for 3 hrs at 4 °C ...
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bioRxiv - Microbiology 2021Quote: ... A 4.5X β-Gal Assay buffer/CPRG solution was made with Chlorophenol Red-β-D-galactopyranoside (CPRG) (Calbiochem) and 4.5X β-Gal Assay buffer (2.475 mL 1M KCl ...
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bioRxiv - Plant Biology 2023Quote: ... Total glutathione and cysteine were quantified by reducing disulfides with DTT followed by thiol derivatization with the fluorescent dye monobromobimane (Thiolyte, Calbiochem). Derivatization was performed as described in Wirtz et al ...
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bioRxiv - Microbiology 2021Quote: ... Anesthetized animals were intranasally infected using 30 to 50 μl of appropriately diluted viruses or PBS containing the indicated amounts of recombinant murine IFN-β (Calbiochem), universal-IFN (PBL assay science ...
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bioRxiv - Developmental Biology 2021Quote: Native membrane proteins were extracted from transfected cells using the ProteoExtract® Native Membrane Protein Extraction Kit (Calbiochem). Proteins from whole cells and from the membrane and soluble fractions were separated on a 12% SDS-PAGE protein gel ...
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bioRxiv - Neuroscience 2020Quote: Mice were submitted to 24 h of fasting and then received ip injections with either saline (0.9% NaCl) or mouse recombinant leptin (5 mg/kg body weight, Calbiochem, Billerica, MA, USA) immediately before the dark cycle ...
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bioRxiv - Cell Biology 2022Quote: ... a proteoExtract protein precipitation kit (Calbiochem, #539180) was used followed by mass spectrometry analysis (Taplin Biological Mass Spectrometry Facility ...
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bioRxiv - Cell Biology 2023Quote: ... Equivalent amounts of protein (1-2 mg) were incubated with 1 μg indicated antibody and 20 μL protein A/G beads (#IP05, Calbiochem) at 4 ℃ overnight ...
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bioRxiv - Molecular Biology 2023Quote: ... Fourty μl Protein A/G Agarose beads (Calbiochem) were added and the samples were pre-cleaned on a rocker-rotator at 4°C for 2 hours ...
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bioRxiv - Microbiology 2020Quote: ... the pro-protein convertase inhibitor Decanoyl-RVKR-CMK (Calbiochem) was applied to cells at a concentration of 5 μM ...
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bioRxiv - Biochemistry 2022Quote: ... The protein extracts were first filtered through Miracloth (CalBiochem) to remove insoluble plant debris and subsequently centrifuged at 5,000 x g for 5 min at 4°C to remove cellular debris and organelles ...
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bioRxiv - Immunology 2021Quote: ... then precipitated with the ProteoExtract Protein Precipitation Kit (Calbiochem). Equal amounts of protein for each set of samples (e.g ...
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bioRxiv - Immunology 2022Quote: ... prior to protein precipitation (Calbiochem Proteo-Extract precipitation kit). Precipitants were resuspended in 1% SDS and quantified by BCA assay for normalization ...
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bioRxiv - Biochemistry 2024Quote: ... Proteins were precipitated out using ProteoExtract precipitation kit (Calbiochem) and dissolved in 1X Laemmli buffer ...
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bioRxiv - Microbiology 2019Quote: ... 20 µL protein A agarose suspension (Calbiochem/Millipore #IP02-1.5ML) was added to each and samples were rocked at 4°C for 2 hours ...
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bioRxiv - Cell Biology 2021Quote: ... and proteins were immunoprecipitated on BRCA2 antibody-conjugated (OP95, Calbiochem) Rec-protein G Sepharose 4B beads (Invitrogen ...
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bioRxiv - Biochemistry 2020Quote: ... Lambda protein phosphatase and alkaline phosphatase were from Calbiochem (#539514-20KV) and Sigma-Aldrich (#P6774-2KU) ...
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bioRxiv - Cancer Biology 2022Quote: ... Purified proteins were dialyzed against 20 mm Tris/HCl (MERCK, Calbiochem, catalogue No ...
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bioRxiv - Neuroscience 2024Quote: ... and 50 µM Rho-associated protein kinase inhibitor (ROCKi; Calbiochem, USA). This media was changed every other day for 6 days ...
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bioRxiv - Cancer Biology 2022Quote: ... Protein A/G plus agarose suspension solution was obtained from Calbiochem (MERCK) and Dimethyl Sulfoxide (DMSO ...
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bioRxiv - Genetics 2021Quote: ... cells were treated with 50 μM of the protein synthesis inhibitor cycloheximide (Calbiochem), or ethanol as vehicle control during 4 h or 8 h ...
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bioRxiv - Bioengineering 2021Quote: ... pH 7.4] and incubated with 60 μL protein G agarose bead slurry (Calbiochem) conjugated to an antibody cocktail containing 24 μg 4G10 (Millipore ...
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bioRxiv - Cell Biology 2021Quote: 20 mg of mitochondria were lysed with digitonin (Calbiochem; 6.6 g/g protein) for 15 min on ice with occasional vortexing and cleared from insoluble material for 20 min at 20,000 × g at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... 10 µM of indicated proteins were mixed with 100 µM nitrocefin (Calbiochem, Sigma) in a reaction buffer containing EstG storage buffer (50 mM HEPES-NaOH pH 7.2 ...
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bioRxiv - Cell Biology 2022Quote: ... followed by the addition 40μl of Protein A or G–Sepharose beads (Calbiochem) and incubated for 4h at 4°C ...
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bioRxiv - Molecular Biology 2020Quote: ... Plates were then washed to remove free protein prior to addition of nitrocefin (Calbiochem) for 1 hour at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... Wnt-surrogate55 (0.15 nM, U-Protein Expression) and Rho kinase inhibitor (10 μM, Calbiochem Y27632) was added to the media whenever cells were at a single-cell stage to help recovery ...
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bioRxiv - Cancer Biology 2022Quote: ... Immunodetection of RAS proteins was carried out with pan-RAS (Ab-3; Calbiochem; 1:1,000) antibodies ...
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bioRxiv - Bioengineering 2020Quote: ... The light/heavy peptide mixture was incubated with 60 μL protein G agarose bead slurry (Calbiochem) conjugated to an antibody cocktail containing 12 μg 4G10 (Millipore) ...
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bioRxiv - Biochemistry 2020Quote: ... all fractions were individually precipitated using the Proteoextract Protein Precipitation Kit (EMD Milipore, Calbiochem, 539180-1KIT). For each 600 µL fraction ...
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bioRxiv - Microbiology 2023Quote: ... The reaction mixture contains 8 μg total proteins and 4 μg nitrocefin (Calbiochem, San Diego, CA). β-Lactamase activity was determined by measuring the absorbance at 486 nm (OD486nm).
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bioRxiv - Cancer Biology 2020Quote: Whole cell protein lysates were prepared in RIPA buffer supplemented with Phosphatase Inhibitor Cocktail Set-II (Calbiochem) and protease inhibitor (VWR ...
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bioRxiv - Neuroscience 2023Quote: ... after which the gels was washed with water and all protein was stained using RAPIDstain (Calbiochem, 553215) for 1 hour ...