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Citations for Calbiochem :
401 - 450 of 484 citations for Recombinant Mouse Frizzled Homolog 1 Drosophila His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... Controls were treated for 30 minutes with KRBH + 2.8 mM glucose + 1 μM latrunculin B (Calbiochem, #428020) or 1 μM jasplakinolide (Millipore Sigma ...
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bioRxiv - Cell Biology 2023Quote: ... GFP-Rab11 WTlow and DNlow cell culture media was also supplemented with 1.25 μg.mL-1 puromycin (Calbiochem). Cells were regularly tested for mycoplasma contamination with the LookOut mycoplasma PCR detection kit (Sigma ...
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bioRxiv - Microbiology 2023Quote: ... cells were pretreated 1 h at 37°C with 20 nM bafilomycin A1 (Calbiochem, stock in DMSO) to block endocytosis ...
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bioRxiv - Neuroscience 2023Quote: ... The following primary antibodies were used: polyclonal rabbit anti-tyrosine hydroxylase (TH, catalog # 657012, 1:1000, Calbiochem), monoclonal mouse anti-TH (catalog # MAB318 ...
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bioRxiv - Neuroscience 2023Quote: ... incubated overnight in TBST with 1% bovine serum albumin (BSA; Calbiochem by Millipore Sigma, St. Louis, MO) and primary antiserum (Millipore Sigma MAB318 ...
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bioRxiv - Neuroscience 2019Quote: ... diluted in KPBS containing 0.3 % Triton X-100 and incubated overnight at RT in cFos rabbit polyclonal antiserum (1:20,000; Calbiochem) diluted in KPBS containing 1 % NGS and 0.3 % Triton X-100 ...
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bioRxiv - Cell Biology 2020Quote: ... and the cells were washed with ice-cold PBS(-/-) with 1X Protease Inhibitor Cocktail Set 1 (Calbiochem 539131). The PBS was aspirated ...
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bioRxiv - Neuroscience 2020Quote: ... 14 – 17 DIV hippocampi cultures were stained live with a GluA1 antibody (GluA1 Calbiochem PC246, rabbit, 1:20) for 15 min at 37°C in a humidified chamber ...
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bioRxiv - Physiology 2021Quote: ... and phosphatase inhibitors (2 μM microcystin-LR, and 1× Phosphatase-Inhibitor Sets I and II (Calbiochem, Hayward, CA). We centrifuged homogenates at 4,000 g for 15 minutes to remove debris ...
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bioRxiv - Biochemistry 2021Quote: ... Cell pellets were sonicated in 20 mM HEPES buffer pH 7.5 containing 0.25 M sucrose and 1:100 protease inhibitor cocktail set V (Calbiochem) and centrifuged at 2,000 × g for 10 min to pellet nuclear fraction ...
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bioRxiv - Biochemistry 2021Quote: ... Data were normalized to maximal inhibition determined by 1 μM decanoyl-Arg-Val-Lys-Arg-chloromethylketone (Calbiochem #344930).
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bioRxiv - Neuroscience 2021Quote: ... or P5–P6 DRG rat neurons were diluted to 35,000 cells/ml in Sato’s media and treated with either 1 mM dbcAMP (Calbiochem), mixed population or recombinant Histones (1-20µg/ml ...
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bioRxiv - Immunology 2020Quote: ... Cells were grown at 37°C to OD = 0.8 and expression was induced with 0.3 mM IPTG (Isopropyl b-D-1-thiogalactopyranoside, Calbiochem) overnight at 18°C ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... sections were incubated with rabbit anti-c-Fos antibody diluted with the blocking solution (1:20000, #PC38, Calbiochem) for 2 days at 4°C ...
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bioRxiv - Molecular Biology 2022Quote: ... Samples were incubated for 1 h with the ADAM17 inhibitor Batimastat (BB-94) at 10 μM (196440, Calbiochem), with the selective ADAM10 inhibitor GI254023X (GI ...
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bioRxiv - Pathology 2023Quote: ... 2% DDM) supplemented with 1:1000 (v/v) Protease Inhibitor Cocktail Set III (Calbiochem, La Jolla, CA, USA). Rat anti-HA (3F10 ...
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bioRxiv - Biophysics 2023Quote: ... and the C-class iPSCs with a mixture of two inhibitors (2i): 1 μM MEK inhibitor (PD0325901, Calbiochem) and 3 μM GSK3 inhibitor (CH99021 ...
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bioRxiv - Molecular Biology 2024Quote: ... The pellet was suspended in 2 ml of lysis/binding buffer (25 mM HEPES/KOH, pH 7.6, 10 % glycerol, 150 mM KCl, 1 mM dithiothreitol, 0.1 % Elugent (Calbiochem), loaded into SHREDDER tubes (Pressure Biosciences Inc ...
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bioRxiv - Cancer Biology 2021Quote: ... and 2 mM glutamine for 24 h prior to treatment with either TGF-β (1 ng/mL, Millipore Calbiochem) or 10 uM TGF-β inhibitors (SB431542 ...
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bioRxiv - Cell Biology 2019Quote: ... cells were lysed at 1 × 109 cells/ml in ice-cold lysis buffer (0.1 µM TLCK, 1 µg/ml Leupeptin, Phosphatase Inhibitor Cocktail II (Calbiochem),1 mM PMSF ...
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bioRxiv - Biochemistry 2019Quote: ... which were grown to an optical density of 0.6–0.8 at 600 nm and were induced with 1 mM isopropyl-β-D-thiogalactopyranoside (CALBIOCHEM) for 20 h at 18 °C ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were rinsed with cold PBS1X and then lysed in Triton-X100 buffer at 4°C (50mM HEPES pH 7.4, 150mM NaCl, 1 % Triton X-100, protease inhibitors (Calbiochem) and phosphatase inhibitor cocktails II and IV (Calbiochem)) ...
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bioRxiv - Cell Biology 2021Quote: ... 50 μg of the total RNA was incubated for 1 h at 4 °C with 10 μl of the anti-2,2,7-trimethylguanosine-antibody (Calbiochem Milipore) coupled to sepharose beads (Fig ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were then harvested using lysis buffer (50mM HEPES pH7.4, 150mM NaCl, 1mM MgCl2, 1mM NaF, 1% Triton-x-100, protease inhibitor cocktail I [Calbiochem) and centrifuged at 14,000rpm for 10 minutes ...
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bioRxiv - Cancer Biology 2022Quote: ... collected and lysed in lysis buffer RGMT (50mM HEPES pH 7.4, 150mM NaCl, 1mM MgCl2, 1mM NaF, 1% Triton-x-100, plus protease inhibitor cocktail I [Calbiochem]). Cellular lysates were cleared by centrifugation and 225µL of total protein extract was incubated with biotin-tagged CIGB-300 (100μM ...
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bioRxiv - Molecular Biology 2022Quote: ... The cell pellet was lysed at 0.5 × 109 cells/ml in ice-cold lysis buffer (0.1 mM TLCK, 1 μg/ml Leupeptin, 1x Phosphatase Inhibitor Cocktail II tablet (Calbiochem), 1 mM PMSF ...
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bioRxiv - Developmental Biology 2022Quote: ... Tissues were homogenized in cold lysis buffer (0.1% NP-40, 50mM Tris-HCl [pH 7.2], 250mM NaCl, 2mM EDTA, phosphatase inhibitor mixture I and II [Calbiochem] and one tablet of Minicomplete [Roche] per 10ml ...
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bioRxiv - Cell Biology 2022Quote: ... alone bound to glutathione sepharose 4B beads in 0.7 ml of binding buffer (1X PBS, 150 mM NaCl, 1% Triton X-100, Calbiochem EDTA-free protease inhibitor cocktail set III ...
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bioRxiv - Immunology 2022Quote: ... and 1 × 106 cells were treated with rapamycin (10 ng/ml, 30 min, 37°C; Calbiochem, San Diego, CA) prior to treatment with rhGH.
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bioRxiv - Molecular Biology 2022Quote: ... pellets were resuspended in 800 μl of ice-cold wash buffer (50 mM NaH2PO4, 300 mM NaCl, 0.05% Tween, 1:200 diluted protease inhibitor cocktail set III (Calbiochem)) supplemented with 0.1 U/μL of recombinant RNase inhibitor (wash buffer-RIn ...
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bioRxiv - Biochemistry 2023Quote: ... Culture was then cooled to 16℃ and induced with 0.5 mM dioxane-free isopropyl B-D-1-thiogalactopyranoside (IPTG) (Calbiochem) overnight at 16℃ ...
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bioRxiv - Biochemistry 2023Quote: ... Ubiquitin was expressed as described above except that the 2 L culture was induced with 0.4 mM dioxane-free isopropyl B-D-1-thiogalactopyranoside (IPTG) (Calbiochem) for four hrs at 37℃ ...
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bioRxiv - Biochemistry 2023Quote: ... Culture was then cooled to 16℃ and induced with 0.4 mM dioxane-free isopropyl B-D-1-thiogalactopyranoside (IPTG) (Calbiochem) overnight at 16℃ ...
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bioRxiv - Biochemistry 2023Quote: ... Culture was then cooled to 16℃ and induced with 0.4 mM dioxane-free isopropyl B-D-1-thiogalactopyranoside (IPTG) (Calbiochem) overnight at 16℃ ...
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bioRxiv - Biophysics 2023Quote: ... or FGFJI (20 ng ml−1, bFGF, R&D Systems; and 200 nM JAK inhibitor, Calbiochem, Merck Millipore, Germany). Cells were collected for mechanical characterization and RNA-Seq after 5-day exposure to the respective culture conditions (30).
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bioRxiv - Cancer Biology 2022Quote: ... Boston Bioproducts (Cat# BP-115X) supplemented with protease inhibitor cocktail at a dilution of 1: 100 (Calbiochem, Cat#539131) and phosphatase inhibitors at a dilution of 1 ...
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bioRxiv - Cell Biology 2023Quote: ... The antibodies (Tables 2 and 3) were diluted according to the tables in PBS containing 1% BSA (126575, Calbiochem).
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bioRxiv - Biochemistry 2023Quote: ... 0.2 mM DTT) supplemented with protease inhibitors (0.1 mM PMSF and 1:500 Cocktail IV (Calbiochem; San Diego, CA), and lysed by two passes through a French press at 1000 psi ...
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bioRxiv - Neuroscience 2023Quote: ... Heads were snap-frozen in liquid nitrogen then homogenized using a mechanical homogenizer in 100 μL TBS (50mM Tris-HCl, pH 8.0) containing 1:200 protease inhibitor cocktail set III (Calbiochem). Homogenates were centrifuged at 16,000 g at 4°C for 30 min ...
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bioRxiv - Molecular Biology 2023Quote: Whole-cell protein extracts were prepared using hot lysis buffer (1%SDS, 5mM EDTA, 50mM Tris, pH7.5) supplemented with protease inhibitor mixture (Calbiochem). Protein samples were diluted and boiled in 5X protein loading buffer (10% SDS ...
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bioRxiv - Neuroscience 2024Quote: ... 1 mM Na3VO4 and 500 nM Cantharidin (#3322.1 Roth)] and commercial protease inhibitors (protease inhibitor cocktail III, #539134, Calbiochem). Phosphatase inhibitors were omitted when Lambda phosphatase treatment was required ...
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bioRxiv - Immunology 2021Quote: ... GMDCs were incubated for 2 h at 37°C with 50 µM MARK inhibitor (MARK/Par-1 Activity Inhibitor, 39621; Calbiochem), 50 nM SIK inhibitor (HG-9-91-01 ...
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bioRxiv - Molecular Biology 2019Quote: ... 0.1 mM PMSF and 0.1 μM Aprotinin) using a Dounce homogenizer (10 strokes, 400 rpm) and filtered through two layers of Mira cloth (Calbiochem). The filtrate was centrifuged at 600g at 4° C for 2 min to remove crud and unbroken cells ...
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bioRxiv - Neuroscience 2019Quote: ... or both hippocampi from a single mouse was homogenized in 2 mL of ice-cold homogenization buffer (0.32 M sucrose, 4 mM HEPES pH 7.4, protease inhibitor cocktail EGTA-free (Calbiochem, 1:1000), RNasin (Promega ...
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bioRxiv - Immunology 2021Quote: The caspase-1 activity was determined with the fluorometric substrate Z-YVAD 7-Amido-4-trifluoromethylcoumarin (Z-YVAD-AFC, caspase-1 substrate VI, Calbiochem), as described previously (Angosto et al. ...
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bioRxiv - Cell Biology 2021Quote: ... The antibodies listed in Table 2 and 3 were diluted according to the tables in PBS containing 1% BSA (126575, Calbiochem).
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bioRxiv - Cell Biology 2022Quote: ... and AR-C118925 {5-[[5-(2,8-dimethyl-5H-dibenzo[a,d]cyclohepten-5-yl)-3,4-dihydro-2-oxo-4-thioxo-1(2H)-pyrimidinyl]methyl]-N-2H-tetrazol-5-yl-2-furancarboxamide} were obtained from Calbiochem-Merck Millipore (Billerica ...
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bioRxiv - Cancer Biology 2022Quote: ... Whole cell samples were ground in liquid nitrogen and lysed in 8 M urea supplemented with 1% protease inhibitor cocktail (Calbiochem). Lysates were centrifuged at 12,000 x g and the supernatant was quantified by BCA assays (Beyotime) ...
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bioRxiv - Microbiology 2022Quote: ... The furin inhibitor decanoyl-RVKR-cmk and mannosidase-1 inhibitor deoxymannojirmycin (DMJ) were purchased from Calbiochem (San Diego, California, USA).
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bioRxiv - Developmental Biology 2021Quote: ... transferred to a 15 ml centrifuge tube containing warm 3 ml 0.5 mg ml−1 collagenase type II (Calbiochem, 234155) solution and incubated for 30 min at 37°C shaker at 180 rpm ...