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Citations for Calbiochem :
351 - 400 of 496 citations for Rat Heat Shock Protein Beta 1 HSPB1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2021Quote: ... Primary antibodies used were rabbit anti-c-Fos (Calbiochem #PC38, 1:1000) or rabbit anti-Cas9 (EnCor Biotechnology ...
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bioRxiv - Cell Biology 2020Quote: ... stained with 1:600 dilution of secondary anti-mouse Alexa 647 (Calbiochem), and then washed three times ...
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bioRxiv - Genomics 2022Quote: ... 1 mM DTT,0.5 mM PMSF (1X Calbiochem Protease lnhibitor cocktail I), 0.25% NP-40 ...
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bioRxiv - Immunology 2022Quote: ... we used PMA (10 ng/mL; Alexis) and ionomycin (1 μM; Calbiochem). In some experiments ...
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bioRxiv - Neuroscience 2022Quote: ... 1 μm of the gamma-secretase inhibitor DAPT (Calbiochem Cat. No. 565784) was added to each well to promote ensheathment ...
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bioRxiv - Cancer Biology 2023Quote: ... and incubated in primary antibodies for PCNA (mouse, 1:200, Calbiochem #PC10) and γH2AX (rabbit ...
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bioRxiv - Cancer Biology 2022Quote: ... and phosphatase inhibitors at a dilution of 1: 500 (Calbiochem, Cat# 524625) for 10 minutes at 4 °C and then centrifuged at 14.000g for 15 minutes (Centrifuge 5415 R) ...
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bioRxiv - Physiology 2024Quote: ... 0.5 mM Na3VO4 and 1x Protease Inhibitor Cocktail Set 1 (PIC) (Calbiochem). Lysates were mixed by pipetting and rotated at 4°C for 20 min then subjected to centrifugation (12000 g for 10 min at 4°C) ...
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bioRxiv - Microbiology 2023Quote: ... or 1 µM of the synthetic Ffar2 agonist (#371725, Calbiochem, Merck Millipore). To inhibit Ffar2 ...
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bioRxiv - Cell Biology 2020Quote: ... with a 1:1000 dilution of yeast protease inhibitor cocktail set IV (Calbiochem), the lysate was cleared by centrifugation at 4C at full speed on a microcentrifuge ...
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bioRxiv - Cell Biology 2021Quote: ... MARK/Par-1 activity inhibitor49 (MARK2i; Calbiochem 39621; 5 µM or 10 µM) was added prior to imaging ...
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bioRxiv - Molecular Biology 2020Quote: ... Primary antibodies were rabbit polyclonal anti-RAD51 (Abcam ab63801, 1:800 or Calbiochem PC130 ...
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bioRxiv - Biochemistry 2020Quote: ... Bound antibodies were detected with peroxidase-labeled anti-rabbit IgG (1:30000) (Calbiochem), and ECL Prime Western Blotting Detection kit (GE Healthcare) ...
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bioRxiv - Microbiology 2021Quote: ... Primary staining was performed for C3a with 1:2,000 rabbit anti-C3a (Calbiochem), for C5b6 with 1:500 dilution of goat-anti human C5 serum (Complement Technology ...
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bioRxiv - Molecular Biology 2022Quote: ... The reaction was started by adding 1 nM to 4 μM calmodulin (Calbiochem) and 100 μM ATP (∼1 Ci mmol-1 [γ32P]-ATP) ...
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bioRxiv - Synthetic Biology 2022Quote: ... 10 mM imidazole and SetIII protease inhibitor-EDTA-free (1:1000 dilution, Calbiochem) were added ...
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bioRxiv - Developmental Biology 2023Quote: ... Enzymatic reaction was blocked by adding PBS+10% FBS+1% DNAse (Calbiochem, 260913) and cell suspension was filtered and centrifuged at 1000 rpm for 5 min ...
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bioRxiv - Developmental Biology 2022Quote: ... we performed terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay according to the manufacturer’s instructions (FragEL™ DNA Fragmentation Detection Kit, Colorimetric-Klenow Enzyme, Calbiochem). Briefly ...
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bioRxiv - Developmental Biology 2020Quote: Apoptotic chondrocytes in frontal knee joint sections were assessed for Terminal deoxynucleotidyltransferase-mediated dUTP Nick End Labeling (TUNEL) using DNA Fragmentation Detection Kit (QIA33-1KT, Calbiochem) and following manufacturer’s instructions.
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bioRxiv - Neuroscience 2022Quote: Cultures were rinsed twice with PBS and cell membrane extracts were collected according to the method of ProteoExtract Kit (Calbiochem). Samples were heated with equal volumes of SDS sample buffer (Novex ...
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bioRxiv - Biochemistry 2020Quote: ... and stable pools were selected with medium containing 500 µg ml-1 G418 (Calbiochem). The expression of the indicated genes in the stable pools was induced by the addition of 1 µg ml-1 doxycycline (Sigma ...
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bioRxiv - Evolutionary Biology 2021Quote: ... blocked (200 μL, PBS 1% BSA wt/vol, Calbiochem #12659-100GM, 3 h, RT) and washed (3x ...
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bioRxiv - Immunology 2020Quote: ... blocked (200 µL, PBS 1% BSA wt/vol, Calbiochem #12659-100GM, 3 h, RT) and washed (3x ...
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bioRxiv - Physiology 2021Quote: ... 0.1% sodium dodecyl sulfate) containing protease inhibitor cocktail (Cocktail set 1, Calbiochem, Bilerica, MA) and phosphatase inhibitors (25 mM NaF ...
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bioRxiv - Cell Biology 2021Quote: ... 3 and 6 hours with the inhibitors for ILK (1 μM CPD022, Calbiochem, #407331), FAK (5 μM FAK inhibitor 14 ...
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bioRxiv - Neuroscience 2020Quote: ... Slices were incubated with polyclonal anti-tyrosine hydroxylase rabbit antibodies (1:1000, Calbiochem, Merck) and were incubated overnight in carrier solution (containing in PBS ...
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bioRxiv - Biochemistry 2023Quote: ... Cells expressing the shRNA constructs were selected using 1 mg/mL Neomycin (Calbiochem, #480100). To induce expression of shRNAs cells were treated for 3 days with 2 µg/mL Dox.
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bioRxiv - Microbiology 2023Quote: ... diluted 5-fold in 100% MeOH containing 1 µM glycocholic acid (Calbiochem, 100.1% pure), and centrifuged for 10 min at 4000 RPM (Thermo Sorvall ST 40R ...
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bioRxiv - Biophysics 2020Quote: ... Cells were stained with Annexin-V-FITC and propidium iodide as indicated in the manual of the annexin V-FITC detection kit (CalbioChem, Darmstadt, Germany) and fluorescence was measured using a FACS Calibur flow cytometer (Becton-Dickinson ...
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bioRxiv - Plant Biology 2019Quote: ... followed by one hour incubation with an anti-rabbit-HRP antibody (dilution 1:10000, Calbiochem). For GFP detection ...
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bioRxiv - Molecular Biology 2020Quote: ... Cells were incubated with primary antibodies against RAD51 (rabbit anti-Rad51 1/200, Calbiochem PC130) and biotin (mouse anti-biotin 1/6,000 ...
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bioRxiv - Neuroscience 2021Quote: ... Tissue samples were homogenized in TritonX-100 and protease inhibitor-containing (1:100, #539131, Calbiochem) Tris-HCl buffer (TBS ...
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bioRxiv - Cell Biology 2021Quote: This study used Latrunculin-A at a final concentration of 1 µM (Calbiochem Cat# 428021), the PAK1 kinase inhibitor ...
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bioRxiv - Neuroscience 2019Quote: ... Sections were incubated overnight (room temperature) with goat anti tyrosine hydroxylase (1:1000, Calbiochem/ Merck) antibody diluted in the carrier solution (0.2 M PBS with 1% horse serum ...
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bioRxiv - Molecular Biology 2021Quote: ... 1% PenStrep) in the presence of selection antibiotic blasticidin S (Blast, 15 μg/ml, Calbiochem). Twenty-four h post plating ...
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bioRxiv - Neuroscience 2021Quote: ... One replicate of each sample was treated with 1 mM conduritol B epoxide (CBE, Calbiochem) for 10 min before all samples were incubated for 1 hr with 2.5 mM of the fluorescent GCase substrate methylumbillifery β-D-glucopyranoside (4MUG ...
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bioRxiv - Biochemistry 2021Quote: ... the cells were incubated with a blocking buffer (1% (w/v) bovine serum albumin (Calbiochem) and 0.5% (w/v ...
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bioRxiv - Immunology 2023Quote: ... Tissue samples were homogenized in TritonX-100 and protease inhibitor-containing (1:100, #539131, Calbiochem) Tris-HCl buffer (TBS ...
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bioRxiv - Cell Biology 2023Quote: ... The WAPL-AID cell line was supplemented with puromycin (0.5 µg mL-1, Calbiochem, 540411). HeLa SMC4-AID_WAPL-dTAG cells were supplemented with blasticidin S (6 µg mL-1 ...
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bioRxiv - Cancer Biology 2022Quote: ... ZBTB18 or ZBTB18-mut as described above or treated with 5 µM RN-1 inhibitor (Calbiochem), for 96h ...
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bioRxiv - Molecular Biology 2020Quote: ... Cells were incubated for 1 h at room temperature with primary antibodies (rabbit anti-RAD51 Calbiochem PC130 1/200 and mouse anti-PCNA Santa Cruz PC10 1/500 ...
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bioRxiv - Cancer Biology 2019Quote: ... Membranes were incubated in 1:1000 diluted primary antibodies pERK1/2 pT202/Y204 (Calbiochem cat# KP26001), total ERK1/2 (Cell Signaling cat# 9102) ...
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bioRxiv - Neuroscience 2019Quote: ... the slices were treated with pronase (1 mg / 4-6 ml) (Calbiochem-MilliporeSigma, Burlington, MA, USA) in incubation medium I at 31°C for 30-50 min ...
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bioRxiv - Cancer Biology 2020Quote: ... Blots were incubated with following primary antibodies at 4 C overnight: TP53 (OP43, Calbiochem, 1:1000), RB1 (Ab-5 ...
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bioRxiv - Cell Biology 2021Quote: ... two other kinase inhibitors were used: 1 μM and 1.5 μM of AKT VIII (Calbiochem #124018) or 100 nM and 200 nM of trametinib (Selleckchem #GSK1120212) ...
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bioRxiv - Neuroscience 2022Quote: ... Nuclei were counterstained with 1 µg/ml of DAPI and sections were mounted with Fluorsave (Calbiochem).
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bioRxiv - Neuroscience 2021Quote: ... #MCA1957) and peptide-rich sensory nerve fibres with a rabbit anti-CGRP (1:2,000, Calbiochem, #PC205L). After washes ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... PamChip arrays (PamGene #88101) were blocked for 20 cycles with 1% Bovine serum albumin (Calbiochem #126609) in Tris-buffered saline (TBS) ...
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bioRxiv - Developmental Biology 2023Quote: Cells were harvested in RIPA buffer with 1:100 protease inhibitor cocktail set I (Calbiochem, #539131) and phosphatase inhibitors (1:100 Na2Ov4 ...
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bioRxiv - Neuroscience 2023Quote: ... protease inhibitors (PI, 1:4000 dilution of protease inhibitor cocktail 3 w/o EDTA, Calbiochem, #539134) and benzonase (1:1000 ...