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Citations for Calbiochem :
401 - 450 of 528 citations for QuantiChrom Arginase Inhibitor Screening Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... The lysate was then combined with 0.5 μL of Protease Inhibitor Cocktail IV (Calbiochem, San Diego, CA) and 4 μL of 25.5 U/μL benzonase nuclease (EMD Millipore ...
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bioRxiv - Immunology 2023Quote: ... NF-κB (BAY11-7082; 2 μM) and RAF1 (RAF1 Kinase inhibitor I; 10 μM) were from Calbiochem. The inhibitor of EGFR (gefitinib ...
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bioRxiv - Microbiology 2024Quote: Parasites were treated with 50 μM of RNA Pol III inhibitor CAS 577784-91-9 (Calbiochem, Merck) after sorbitol treatment at 3 ±3hpi and RNA was harvested at 24hpi in parallel with untreated and control samples ...
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bioRxiv - Biochemistry 2022Quote: ... ScDIP2-GFP knock-in cells were resuspended in a cell resuspension buffer (50 mM Tris/HCl, pH 7.6, 0.6 M sorbitol, 1mM EDTA) containing protease inhibitor cocktail (Calbiochem) and 1mM PMSF (Sigma) ...
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bioRxiv - Biochemistry 2020Quote: Pelleted cells were resuspended in 30 mL Trit buffer (PBS pH 7.4 supplemented with 150 mM NaCl, 0.05% Triton X100) and Protease Inhibitor Cocktail (Calbiochem). Cells were lysed on ice with sonication after which DNAse I (1 μL ...
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bioRxiv - Neuroscience 2020Quote: ... Total mouse hippocampus from the left hemisphere was homogenised using a mechanical homogeniser and disposable pestles (Anachem) in 100μl TBS (50mM Tris-HCl pH 8.0) containing protease and phosphatase inhibitors (Calbiochem). The homogenate was then centrifuged at 4 °C for 30 mins at 16,000 × g ...
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bioRxiv - Neuroscience 2020Quote: ... beads were incubated with astrocyte lysate buffer supplemented with protease inhibitors (Merck, Calbiochem set III, Cat. No. 539134) for one hour at 4°C on a rotating wheel ...
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bioRxiv - Cancer Biology 2022Quote: The cells were lysed in radioimmunoprecipitation assay buffer (RIPA buffer) supplemented with phosphatase inhibitor cocktail set-II (Calbiochem) and protease inhibitor (VWR) ...
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bioRxiv - Plant Biology 2021Quote: ... 0.1% sodium ascorbate and protease inhibitors) using polytron (Kinematica) and filtered through two layers of Miracloth (Calbiochem, # 475855). The filtrate was centrifuged at 5000 rpm for 5 minutes and the pellet was resuspended in 0.4ml of XpI buffer ...
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bioRxiv - Molecular Biology 2020Quote: WT and endogenous TcZC3H12-HA expressing epimastigotes were incubated with 10 μg/mL proteasome inhibitor (MG-132 - Calbiochem) for one hour at 28° C ...
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bioRxiv - Cell Biology 2020Quote: ... and the cells were washed with ice-cold PBS(-/-) with 1X Protease Inhibitor Cocktail Set 1 (Calbiochem 539131). The PBS was aspirated ...
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bioRxiv - Biochemistry 2021Quote: ... Cell pellets were sonicated in 20 mM HEPES buffer pH 7.5 containing 0.25 M sucrose and 1:100 protease inhibitor cocktail set V (Calbiochem) and centrifuged at 2,000 × g for 10 min to pellet nuclear fraction ...
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bioRxiv - Cell Biology 2020Quote: ... 1 mM EDTA and 1% Triton X-100) supplemented with protease inhibitor cocktail V (Calbiochem, San Diego, CA) and 1 mM phenylmethylsulfonyl fluoride (PMSF) ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: Brain tissue was homogenized in RIPA buffer supplemented with protease and protein phosphatase inhibitors (Calbiochem, La Jolla, CA) and subjected to SDS-PAGE followed by transferring to PVDF membranes (Millipore ...
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bioRxiv - Molecular Biology 2022Quote: ... Samples were incubated for 1 h with the ADAM17 inhibitor Batimastat (BB-94) at 10 μM (196440, Calbiochem), with the selective ADAM10 inhibitor GI254023X (GI ...
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bioRxiv - Pathology 2023Quote: ... 2% DDM) supplemented with 1:1000 (v/v) Protease Inhibitor Cocktail Set III (Calbiochem, La Jolla, CA, USA). Rat anti-HA (3F10 ...
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bioRxiv - Cell Biology 2023Quote: ... mouse organs were homogenized in 2% SDS lysis buffer containing protease inhibitor mixture III (# 535140, Calbiochem, Billerica, MA) and phosphatase inhibitors (#P2850 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... collected pRPE cells were suspended in a hypotonic buffer containing protease inhibitor cocktail (MilliporeSigma™ Calbiochem™, 53913110VL). The pRPE cells underwent disruption with a Dounce tissue homogenizer ...
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bioRxiv - Physiology 2023Quote: Total protein extracts were obtained from microalgal cells in exponential phase of growth (e.g. 4 days) in 50 mM Tris buffer pH 8.0 supplemented with protein inhibitor cocktail (539131, Calbiochem). Free-living and symbiotic microalgae were disrupted by bead beating with a Precellys device (Bertin Technologies ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were treated with 10 nm of estradiol-17β or 10 μM γ-secretase inhibitor IX (DAPT; Calbiochem/EMD Millipore #565770-5MG ...
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bioRxiv - Genetics 2021Quote: 48 hpf zebrafish larvae were treated for 24 hours with 10 µM and 50 µM ATR Inhibitor IV (Calbiochem) or DMSO as negative control ...
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bioRxiv - Cell Biology 2019Quote: ... cells were lysed at 1 × 109 cells/ml in ice-cold lysis buffer (0.1 µM TLCK, 1 µg/ml Leupeptin, Phosphatase Inhibitor Cocktail II (Calbiochem),1 mM PMSF ...
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bioRxiv - Physiology 2021Quote: ... cells were treated with poly(I:C) (1.5 μg/mL for 4 hrs) and a combination of proteasome inhibitor (Bortezomib, 2 μM, Sigma/Calbiochem 5043140001) and prolyl endopeptidase inhibitors (Pramiracetam 10 uM ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were rinsed with cold PBS1X and then lysed in Triton-X100 buffer at 4°C (50mM HEPES pH 7.4, 150mM NaCl, 1 % Triton X-100, protease inhibitors (Calbiochem) and phosphatase inhibitor cocktails II and IV (Calbiochem)) ...
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bioRxiv - Microbiology 2020Quote: ... In brief, lysis buffer (8 M urea, 1% Triton-100, 10 mM dithiothreitol, and 1% Protease Inhibitor Cocktail (Calbiochem) was used to extract the total protein ...
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bioRxiv - Neuroscience 2020Quote: ... A half cortex was weighed on a microscale and homogenised in 4 volumes of ice-cold Tris-buffered saline (TBS) (50mM Tris-HCl pH 8.0) containing a cocktail of protease and phosphatase inhibitors (Calbiochem) using a handheld mechanical homogeniser and disposable pestles (Anachem) ...
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bioRxiv - Neuroscience 2020Quote: ... in approximately 5 volumes of ice-cold Tris-buffered saline (TBS) (50 mM Tris-HCL, pH 8.0) containing a cocktail of protease and phosphatase inhibitors (Calbiochem). Homogenates were centrifuged at 16,000 x g at 4 °C for 30 min ...
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bioRxiv - Neuroscience 2020Quote: Weighed cortex was homogenised in Tris-buffered saline (TBS) (50 mM Tris-HCl pH 8.0) plus complete protease and phosphatase inhibitors (Calbiochem) before centrifugation at 175,000 × g for 30 minutes at 4°C ...
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bioRxiv - Neuroscience 2022Quote: ... A half cortex was weighed on a microscale and homogenised in 4 volumes of ice-cold Tris-buffered saline (TBS) (50mM Tris-HCl pH 8.0) containing a cocktail of protease and phosphatase inhibitors (Calbiochem) using a handheld mechanical homogeniser and disposable pestles (Anachem) ...
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bioRxiv - Neuroscience 2022Quote: A half cortex was weighed on a microscale and homogenised in 3 volumes of ice-cold Tris-buffered saline (TBS) (50mM Tris-HCl pH 8.0) containing a cocktail of protease and phosphatase inhibitors (Calbiochem) using a handheld mechanical homogeniser and disposable pestles (Anachem ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were then harvested using lysis buffer (50mM HEPES pH7.4, 150mM NaCl, 1mM MgCl2, 1mM NaF, 1% Triton-x-100, protease inhibitor cocktail I [Calbiochem) and centrifuged at 14,000rpm for 10 minutes ...
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bioRxiv - Cancer Biology 2022Quote: ... collected and lysed in lysis buffer RGMT (50mM HEPES pH 7.4, 150mM NaCl, 1mM MgCl2, 1mM NaF, 1% Triton-x-100, plus protease inhibitor cocktail I [Calbiochem]). Cellular lysates were cleared by centrifugation and 225µL of total protein extract was incubated with biotin-tagged CIGB-300 (100μM ...
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bioRxiv - Immunology 2022Quote: Cell samples or EV samples were lysed in radioimmunoprecipitation assay (RIPA) buffer supplemented with protease inhibitors (Calbiochem, CA, USA) and phosphatase inhibitors (Calbiochem) ...
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bioRxiv - Molecular Biology 2022Quote: ... The cell pellet was lysed at 0.5 × 109 cells/ml in ice-cold lysis buffer (0.1 mM TLCK, 1 μg/ml Leupeptin, 1x Phosphatase Inhibitor Cocktail II tablet (Calbiochem), 1 mM PMSF ...
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bioRxiv - Biophysics 2022Quote: ... Cell pellets were lysed with an Emulsiflex (Avastin) in the presence of protease inhibitor cocktail Mixture Set V (Calbiochem) and DNAse (Millipore Sigma) ...
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bioRxiv - Developmental Biology 2022Quote: ... Tissues were homogenized in cold lysis buffer (0.1% NP-40, 50mM Tris-HCl [pH 7.2], 250mM NaCl, 2mM EDTA, phosphatase inhibitor mixture I and II [Calbiochem] and one tablet of Minicomplete [Roche] per 10ml ...
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bioRxiv - Neuroscience 2021Quote: ... cells were lysed in mild non-ionic detergents (0.1% Triton X-100 and Deoxycholate) and protease inhibitor cocktail (Calbiochem) to preserve the integrity of histones harbouring epitopes of interest during cell lysis ...
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bioRxiv - Neuroscience 2022Quote: ... 1.33 mM Ca2+ and 1 mM of the cAMP phosphodiesterase inhibitor 3-isobutyl-1-methylxanthine (IBMX: Calbiochem, Damstard, Germany) were added to the synaptosomes for 15 min ...
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bioRxiv - Developmental Biology 2020Quote: ... Chemical inhibitors were added at the beginning of the 24 hour culture period at following final concentrations: SU5402 (Calbiochem) at 40 μM ...
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bioRxiv - Immunology 2019Quote: ... Some of ITGB4−/− mice were given intraperitoneal (i.p.) injections of EGFR inhibitor AG1478 (20mg/kg, Calbiochem, San Diego, CA) which was dissolved in CMC-Na (0.5% ...
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bioRxiv - Plant Biology 2022Quote: ... except that the resuspension of protein precipitate was in modified denaturing urea buffer (8 M urea, 2% SDS, 100 mM HEPES-KOH pH 7.4, 10 mM EDTA and protease inhibitor [Calbiochem, now Merck ...
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bioRxiv - Microbiology 2022Quote: ... two reactions with increasing dosages of the PfCKII-α-specific inhibitors 4,5,6,7-tetramethylbromobenzotriazole and 4,5,6,7-tetramethylbromo-2-azabenzimidazole (TBB) (Merck/Calbiochem CAS 17374-26-4) were performed to check for possible suppression of phosphoryl group transfer ...
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bioRxiv - Molecular Biology 2022Quote: ... pellets were resuspended in 800 μl of ice-cold wash buffer (50 mM NaH2PO4, 300 mM NaCl, 0.05% Tween, 1:200 diluted protease inhibitor cocktail set III (Calbiochem)) supplemented with 0.1 U/μL of recombinant RNase inhibitor (wash buffer-RIn ...
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bioRxiv - Biophysics 2023Quote: ... or FGFJI (20 ng ml−1, bFGF, R&D Systems; and 200 nM JAK inhibitor, Calbiochem, Merck Millipore, Germany). Cells were collected for mechanical characterization and RNA-Seq after 5-day exposure to the respective culture conditions (30).
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bioRxiv - Cancer Biology 2022Quote: ... Boston Bioproducts (Cat# BP-115X) supplemented with protease inhibitor cocktail at a dilution of 1: 100 (Calbiochem, Cat#539131) and phosphatase inhibitors at a dilution of 1 ...
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bioRxiv - Biochemistry 2023Quote: ... 0.2 mM DTT) supplemented with protease inhibitors (0.1 mM PMSF and 1:500 Cocktail IV (Calbiochem; San Diego, CA), and lysed by two passes through a French press at 1000 psi ...
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bioRxiv - Neuroscience 2023Quote: ... Heads were snap-frozen in liquid nitrogen then homogenized using a mechanical homogenizer in 100 μL TBS (50mM Tris-HCl, pH 8.0) containing 1:200 protease inhibitor cocktail set III (Calbiochem). Homogenates were centrifuged at 16,000 g at 4°C for 30 min ...
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bioRxiv - Molecular Biology 2023Quote: Whole-cell protein extracts were prepared using hot lysis buffer (1%SDS, 5mM EDTA, 50mM Tris, pH7.5) supplemented with protease inhibitor mixture (Calbiochem). Protein samples were diluted and boiled in 5X protein loading buffer (10% SDS ...
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bioRxiv - Genetics 2021Quote: ... The larvae were individualized in each well of 96-well plates containing ATR Inhibitor IV (CAS 1232410-49-10 Calbiochem) at 10 µM or DMSO in egg water as negative control ...
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bioRxiv - Physiology 2019Quote: ... Tissues were homogenized in 20 mM HEPES buffer (pH 7.5) containing 0.25M sucrose and a cocktail of protease inhibitors (Calbiochem, Burlington, MA) followed by ultracentrifugation at 100,000g for 45 min ...