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Citations for Calbiochem :
151 - 200 of 269 citations for PAS Domain Containing Serine threonine Protein Kinase PASK Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: ... The blots were washed with PBS and incubated with primary antibodies (anti-EBV gH/gL (a rabbit polyclonal antiserum;1:200) (45) and anti-His tag antibody (OB05, Calbiochem, 1:1000) for His-tagged KSHV gL ...
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bioRxiv - Plant Biology 2020Quote: ... secondary antibody: anti-rabbit HRP (1:10,000, Calbiochem #401353)).
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bioRxiv - Cell Biology 2021Quote: ... and GAPDH antibody (CB1001, Calbiochem, mouse monoclonal, 1:5000). For Western blots of RlucC-ER and Mito-RlucN ...
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bioRxiv - Cancer Biology 2019Quote: ... mouse monoclonal anti-BRCA1 antibody (OP92, Ab-1; Calbiochem, for IPs 10μg/ml ...
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bioRxiv - Molecular Biology 2020Quote: ... Antibodies against α-tubulin were purchased from Calbiochem (CP06), GFP and for NDUFS3 from Abcam (ab6556 and ab14711 respectively) ...
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bioRxiv - Microbiology 2019Quote: ... GAPDH was detected with mouse monoclonal antibody 6C5 (Calbiochem). SAG1 levels were used to control for the levels of parasites within the infected cells and blots were stained with polyclonal rabbit anti-SAG1 followed by a secondary goat anti-Rabbit antibody conjugated to HRP ...
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bioRxiv - Neuroscience 2022Quote: ... mouse anti-CC1 antibody (1:500, Calbiochem, cat# OP80), mouse anti MBP Antibody (1:1000 ...
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bioRxiv - Cell Biology 2023Quote: ... a neutralizing antibody against E-cadherin (SHE78-7, Calbiochem) was used at the final concentration of either 1 or 3 µm/ml ...
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bioRxiv - Cancer Biology 2022Quote: ... Immunodetection of RAS proteins was carried out with pan-RAS (Ab-3; Calbiochem; 1:1,000) antibodies ...
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bioRxiv - Genetics 2021Quote: ... The larvae were individualized in each well of 96-well plates containing ATR Inhibitor IV (CAS 1232410-49-10 Calbiochem) at 10 µM or DMSO in egg water as negative control ...
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bioRxiv - Physiology 2019Quote: ... Tissues were homogenized in 20 mM HEPES buffer (pH 7.5) containing 0.25M sucrose and a cocktail of protease inhibitors (Calbiochem, Burlington, MA) followed by ultracentrifugation at 100,000g for 45 min ...
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bioRxiv - Molecular Biology 2019Quote: ... Transformants were regenerated and selected on regeneration medium containing 100 µg/ml of hygromycin B (Calbiochem, La Jolla, CA, USA) and 150 µg/ml G418 sulfate (Cellgro ...
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bioRxiv - Systems Biology 2022Quote: ... respectively) were resuspended in 1 mL of ChIP buffer containing 5 μl of protease inhibitor cocktail (CalbioChem, cat # 539134-1) (50 mM Tris-HCl pH 8 ...
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bioRxiv - Developmental Biology 2021Quote: ... transferred to a 15 ml centrifuge tube containing warm 3 ml 0.5 mg ml−1 collagenase type II (Calbiochem, 234155) solution and incubated for 30 min at 37°C shaker at 180 rpm ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... containing 100 µg streptomycin ml−1 and 100 U penicillin ml−1 using neomycin (400 µg/ml) (Calbiochem, EMD Chemicals) as a selection marker ...
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bioRxiv - Microbiology 2019Quote: ... Transformants were regenerated and selected on regeneration medium containing 100 µg/ml of hygromycin B (Calbiochem, La Jolla, CA, USA) and/or 150 µg/ml G418 sulfate (Cellgro ...
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bioRxiv - Cell Biology 2021Quote: ... Strains expressing both CDC11A-mCherry-NAT and CDC11B-GFP-NEO were constructed by transforming AG230 with a 7678bp fragment from PciI/NotI/PspXI digested AGB1320 and selecting on media containing 100ug/ml G418 (CalBiochem). Transformants were verified by PCR ...
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bioRxiv - Genomics 2019Quote: ... Pulverized material was thawed in glycerol containing nuclear isolation buffer to stabilize nuclear structure and then filtered through Miracloth (Calbiochem) to remove large tissue debris ...
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bioRxiv - Cell Biology 2022Quote: ... Forty-eight hours post transfection cells were removed and incubated in 250 μL/well of HEK Lysis Buffer containing protease Inhibitor Cocktail Set V (Calbiochem) before being centrifuged at 13 000 rpm for 10 min ...
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bioRxiv - Cell Biology 2023Quote: ... cells were washed 3 times with PBS-T and glass coverslips were added on cells (mitosis) or coverslips were mounted onto slides with Mowiol containing DAPI (Calbiochem).
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bioRxiv - Molecular Biology 2024Quote: ... the transfected cells were split and plated into three 10-cm dishes in culture medium containing selection antibiotics blasticidin S (15 μg/ml, Calbiochem) and hygromycin B (100 μg/ml ...
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bioRxiv - Neuroscience 2020Quote: ... rabbit polyclonal antibody against c-Fos (1:500; PC38T, Calbiochem). Secondary antibodies were anti-isotypic Alexa Fluor conjugates (1:500 ...
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bioRxiv - Neuroscience 2019Quote: ... for stainings with synapsin and rabbit GluR1 antibody (Calbiochem, pc246) for the internalization assay ...
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bioRxiv - Cell Biology 2021Quote: ... primary antibodies: GAPDH (Calbiochem; clone 6C5; #CB1001; 1:10,000 dilution), Cdc42 (Cell Signaling Technology ...
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bioRxiv - Molecular Biology 2023Quote: ... 3.3 μg/ml of rabbit anti-Myc primary antibody (Calbiochem) was incubated for 1 h at room temperature (RT ...
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bioRxiv - Cell Biology 2023Quote: ... mouse monoclonal anti-transferrin receptor antibody was from Calbiochem (#GRO9L); anti-mouse and anti-rabbit Cy3 conjugated secondary antibodies were from Amersham ...
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bioRxiv - Bioengineering 2020Quote: ... The light/heavy peptide mixture was incubated with 60 μL protein G agarose bead slurry (Calbiochem) conjugated to an antibody cocktail containing 12 μg 4G10 (Millipore) ...
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bioRxiv - Biochemistry 2020Quote: ... all fractions were individually precipitated using the Proteoextract Protein Precipitation Kit (EMD Milipore, Calbiochem, 539180-1KIT). For each 600 µL fraction ...
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bioRxiv - Microbiology 2023Quote: ... The reaction mixture contains 8 μg total proteins and 4 μg nitrocefin (Calbiochem, San Diego, CA). β-Lactamase activity was determined by measuring the absorbance at 486 nm (OD486nm).
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bioRxiv - Cell Biology 2019Quote: ... 2-3 × 105 single cells EpiSCs were seeded on 5 μg/10 cm2 fibronectin-coated 6-well plates in media containing (1 μl/ml) ROCK inhibitor (InSolution™ Y-276320, Cat.#688001-500UG, VWR Calbiochem). In the morning of transfection ...
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bioRxiv - Microbiology 2021Quote: ... Anesthetized animals were intranasally infected using 30 to 50 μl of appropriately diluted viruses or PBS containing the indicated amounts of recombinant murine IFN-β (Calbiochem), universal-IFN (PBL assay science ...
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bioRxiv - Microbiology 2019Quote: ... and scraped into 100 uL ice-cold RIPA lysis buffer containing 1X protease inhibitor cocktail III (Calbiochem, EMD Millipore, Darmstadt, Germany) and 1X PhosSTOP phosphatase inhibitor cocktail (Roche ...
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bioRxiv - Molecular Biology 2023Quote: ... cultures were lysed with a solution containing SYBR green (1:5000, Invitrogen Cat. No. S7567) (Lysis buffer: 0.16% saponin (Calbiochem/EMD #558255), 0.05 M TRIS-HCl (Roche Cat ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Cells were then lysed in Pierce™ IP Lysis Buffer (87788, ThermoFischer Scientific, Rockford, USA) containing proteases (539138, Calbiochem, Darmstadt, Germany) and phosphatases inhibitors (524629 ...
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bioRxiv - Molecular Biology 2024Quote: ... cells were released from the arrest by transferring them to α-factor-free medium containing 150 μg/mL of Pronase (Calbiochem) and cultured for 30 min ...
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bioRxiv - Immunology 2019Quote: Total Cell lysates were prepared using NP40 lysis buffer (150mM NaCl, 0.5%NP40, 50mM Tris, pH 8.0) containing protease inhibitors (Protease V cocktail, Calbiochem or Halt-protease, Invitrogen), 1mM sodium orthovanadate (Sigma) ...
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bioRxiv - Neuroscience 2019Quote: Pre-sorted synaptosomes (~750 μl) on iced were mixed with ice cold PBS containing protease inhibitor cocktail EGTA-free (Calbiochem, 1:1000) and RNasin (Promega ...
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bioRxiv - Immunology 2021Quote: B cell staining was performed on frozen PBMCs after thawing in complete medium (RPMI 10% FBS, PS/G 1X) containing DNase I (Calbiochem, cod 260913) for 10 min at 37°C ...
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bioRxiv - Cancer Biology 2020Quote: Whole cell protein lysates were prepared in RIPA buffer supplemented with Phosphatase Inhibitor Cocktail Set-II (Calbiochem) and protease inhibitor (VWR ...
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bioRxiv - Neuroscience 2023Quote: ... after which the gels was washed with water and all protein was stained using RAPIDstain (Calbiochem, 553215) for 1 hour ...
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bioRxiv - Microbiology 2020Quote: ... membranes were incubated with primary antibodies as follows: RB1 (Calbiochem/EMD), actin (Millipore) ...
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bioRxiv - Microbiology 2021Quote: ... 50 μL mouse monoclonal anti-6X His tagged primary antibody (Calbiochem) was added at a dilution of 1:150 in 0.5 % BSA in PBST in the respective wells and incubated for 2 hours without shaking at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... GAPDH antibody (clone #6C5) was purchased from Calbiochem (Merck-Millipore, Germany), beta-actin antibody (ab8227 ...
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bioRxiv - Genetics 2020Quote: ... The following antibodies were used: mouse anti-KDEL (Calbiochem, 1:500), mouse anti-GM-130 (BD Transduction Laboratories ...
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bioRxiv - Microbiology 2021Quote: ... GAPDH was detected using mouse monoclonal anti-GAPDH antibody 6C5 (Calbiochem), and biotinylated proteins were detected using streptavidin-HRP (Invitrogen S911) ...
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bioRxiv - Neuroscience 2022Quote: ... Primary antibodies were diluted in TBS + 0.1% Tween + 5% BSA (Calbiochem). After several washes in TBS + 0.1% Tween ...
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bioRxiv - Cancer Biology 2023Quote: ... primary antibody for BRCA1 (Clone MS110, Calbiochem Cat# OP92, Darmstadt, Germany) was used at 1:100 dilution and incubated for 1 hour at room temperature ...
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bioRxiv - Plant Biology 2020Quote: ... The peels were then transferred into 10-ml buffer (Araújo et al., 2011) containing the enzyme CELLULYSIN cellulase from Trichoderma viride (Calbiochem, La Jolla, CA, USA) and digested for 1 h at a shaking speed of 150 rpm ...
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bioRxiv - Genetics 2022Quote: ... an aliquot of the cushion supernatant and eluate were precipitated using the ProteoExtract protein precipitation kit (Calbiochem 539180) per manufacturer protocol and redissolved in 2X Laemmli buffer (Fisher 50-196-784) ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: Brain tissue was homogenized in RIPA buffer supplemented with protease and protein phosphatase inhibitors (Calbiochem, La Jolla, CA) and subjected to SDS-PAGE followed by transferring to PVDF membranes (Millipore ...