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Citations for Calbiochem :
151 - 200 of 602 citations for Mouse Starch Binding Domain Containing Protein 1 STBD1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... GAPDH was detected using mouse monoclonal anti-GAPDH antibody 6C5 (Calbiochem), and biotinylated proteins were detected using streptavidin-HRP (Invitrogen S911) ...
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bioRxiv - Cancer Biology 2021Quote: ... and then lysed with radio-immunoprecipitation assay (RIPA) buffer containing protease and phosphatase inhibitor cocktail (Calbiochem). The protein concentrations of cell lysate supernatants were assayed using a BCA protein assay kit (Thermo Scientific) ...
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bioRxiv - Cancer Biology 2022Quote: ... Protein A/G plus agarose suspension solution was obtained from Calbiochem (MERCK) and Dimethyl Sulfoxide (DMSO ...
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bioRxiv - Biochemistry 2021Quote: ... whereupon the final two wash steps were replaced with a single wash in 1.0 ml of 5 mM sodium phosphate buffer (pH 7.4) containing 0.1% (w/v) sarkosyl (Calbiochem) and 0.3% (w/v ...
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bioRxiv - Biophysics 2023Quote: Pharmacologically treated cells were respectively incubated in OM containing 500 nM latrunculin B (Calbiochem, Millipore, Billerica, MA), 20 U/mL cholesterol oxidase (Calbiochem ...
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bioRxiv - Cell Biology 2021Quote: ... Mouse monoclonal antibodies against human Hsp90α (CA1023) purchased from Calbiochem (Billerica, MA) and Stressmarq Biosciences (Victoria ...
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bioRxiv - Microbiology 2019Quote: ... and GAPDH was detected using mouse monoclonal anti-GAPDH antibody 6C5 (Calbiochem). Horseradish peroxidase (HRP ...
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bioRxiv - Cell Biology 2023Quote: ... LAMP1 (rat, Developmental Studies Hybridoma Bank 1D4B) and Tubulin-□ (mouse, Calbiochem cp06) primary antibodies were diluted 1:200 and 1:500 respectively in TBS plus 1% normal donkey serum and 0.1% Triton X-100 ...
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bioRxiv - Genetics 2021Quote: ... cells were treated with 50 μM of the protein synthesis inhibitor cycloheximide (Calbiochem), or ethanol as vehicle control during 4 h or 8 h ...
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bioRxiv - Bioengineering 2021Quote: ... pH 7.4] and incubated with 60 μL protein G agarose bead slurry (Calbiochem) conjugated to an antibody cocktail containing 24 μg 4G10 (Millipore ...
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bioRxiv - Cell Biology 2021Quote: 20 mg of mitochondria were lysed with digitonin (Calbiochem; 6.6 g/g protein) for 15 min on ice with occasional vortexing and cleared from insoluble material for 20 min at 20,000 × g at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... 10 µM of indicated proteins were mixed with 100 µM nitrocefin (Calbiochem, Sigma) in a reaction buffer containing EstG storage buffer (50 mM HEPES-NaOH pH 7.2 ...
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bioRxiv - Cell Biology 2022Quote: ... followed by the addition 40μl of Protein A or G–Sepharose beads (Calbiochem) and incubated for 4h at 4°C ...
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bioRxiv - Cell Biology 2019Quote: ... 5 mM MgCl2 reaction buffer containing 0.015% w/v 5-bromo-4-chloro-3-indolyl phosphate-BCIP-(Calbiochem) substrate and 0.03% w/v nitro blue tetrazolium-NBT-(BDH Chemicals Ltd. ...
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bioRxiv - Biochemistry 2022Quote: ... ScDIP2-GFP knock-in cells were resuspended in a cell resuspension buffer (50 mM Tris/HCl, pH 7.6, 0.6 M sorbitol, 1mM EDTA) containing protease inhibitor cocktail (Calbiochem) and 1mM PMSF (Sigma) ...
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bioRxiv - Neuroscience 2020Quote: ... Total mouse hippocampus from the left hemisphere was homogenised using a mechanical homogeniser and disposable pestles (Anachem) in 100μl TBS (50mM Tris-HCl pH 8.0) containing protease and phosphatase inhibitors (Calbiochem). The homogenate was then centrifuged at 4 °C for 30 mins at 16,000 × g ...
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bioRxiv - Neuroscience 2023Quote: ... we used a pipette solution containing 50-100 ng/ml amphotericin B as permeabilizing agent (Calbiochem, Madrid, Spain) and a pipette-filling solution containing (in mM) ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... collected pRPE cells were suspended in a hypotonic buffer containing protease inhibitor cocktail (MilliporeSigma™ Calbiochem™, 53913110VL). The pRPE cells underwent disruption with a Dounce tissue homogenizer ...
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bioRxiv - Microbiology 2020Quote: ... mouse monoclonal IgG2a anti-SV40-LTag cross-reacting with BKPyV LTag (PAb416, Calbiochem), mouse monoclonal IgG1 anti-BKPyV Vp1 (MAB3204-M19 ...
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bioRxiv - Molecular Biology 2020Quote: ... Plates were then washed to remove free protein prior to addition of nitrocefin (Calbiochem) for 1 hour at room temperature ...
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bioRxiv - Cancer Biology 2021Quote: Cell pellets were sonicated in ice-cold lysis buffer (containing 20 mM Tris-HCl, pH 7.4, 5 mM EDTA, 10 mM Na4P2O7 (Calbiochem), 100 mM NaF ...
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bioRxiv - Cancer Biology 2021Quote: Cell cultures in 10cm dishes were scrape-harvested in 500 μL lysis buffer containing 0.2% (v/v) protease inhibitors (Protease Inhibitor Cocktail set III, Calbiochem). Lysis buffer comprised ...
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bioRxiv - Immunology 2020Quote: ... Fixed cells were washed three times in PBS and permeabilized by incubating them in PBS containing 5% non-fat milk and 0.05% saponin (Calbiochem). Coverslips were placed in primary antibody for 1 hour then washed 3x in PBS and placed in secondary antibody ...
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bioRxiv - Neuroscience 2020Quote: ... A half cortex was weighed on a microscale and homogenised in 4 volumes of ice-cold Tris-buffered saline (TBS) (50mM Tris-HCl pH 8.0) containing a cocktail of protease and phosphatase inhibitors (Calbiochem) using a handheld mechanical homogeniser and disposable pestles (Anachem) ...
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bioRxiv - Neuroscience 2020Quote: ... in approximately 5 volumes of ice-cold Tris-buffered saline (TBS) (50 mM Tris-HCL, pH 8.0) containing a cocktail of protease and phosphatase inhibitors (Calbiochem). Homogenates were centrifuged at 16,000 x g at 4 °C for 30 min ...
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bioRxiv - Neuroscience 2022Quote: ... A half cortex was weighed on a microscale and homogenised in 4 volumes of ice-cold Tris-buffered saline (TBS) (50mM Tris-HCl pH 8.0) containing a cocktail of protease and phosphatase inhibitors (Calbiochem) using a handheld mechanical homogeniser and disposable pestles (Anachem) ...
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bioRxiv - Neuroscience 2022Quote: A half cortex was weighed on a microscale and homogenised in 3 volumes of ice-cold Tris-buffered saline (TBS) (50mM Tris-HCl pH 8.0) containing a cocktail of protease and phosphatase inhibitors (Calbiochem) using a handheld mechanical homogeniser and disposable pestles (Anachem ...
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bioRxiv - Biochemistry 2021Quote: ... A custom compound library containing over 5000 compounds assembled from commercial sources (Sigma, Selleck, Enzo, Tocris, Calbiochem, and Symansis) were distributed in 24 plates using an Echo 550 (Labcyte ...
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bioRxiv - Cell Biology 2023Quote: ... cells were washed 3 times for 8 min in PBS-Triton 0.01% with rocking in the dark and glass coverslips were mounted on glass slides using Mowiol containing 0.75 μg/ml DAPI (Calbiochem) and imaged with a 100x or 63x objective using Zeiss epifluorescence microscope ...
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bioRxiv - Neuroscience 2020Quote: ... Mouse sections were mounted on glass slides and coverslipped with Mowiol (Calbiochem, Cat. 475904). Images were captured using an Olympus BX41 microscope with an Olympus camera DP-70 (Olympus Denmark A/S).
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bioRxiv - Cell Biology 2024Quote: ... anti-USP14 and HSC70 anti-mouse IgM-HRP conjugate (Calbiochem, Cat# 401225,1:10,000 dilution). Image Studio software (Licor ...
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bioRxiv - Cell Biology 2020Quote: ... Wnt-surrogate55 (0.15 nM, U-Protein Expression) and Rho kinase inhibitor (10 μM, Calbiochem Y27632) was added to the media whenever cells were at a single-cell stage to help recovery ...
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bioRxiv - Genetics 2021Quote: ... The larvae were individualized in each well of 96-well plates containing ATR Inhibitor IV (CAS 1232410-49-10 Calbiochem) at 10 µM or DMSO in egg water as negative control ...
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bioRxiv - Physiology 2019Quote: ... Tissues were homogenized in 20 mM HEPES buffer (pH 7.5) containing 0.25M sucrose and a cocktail of protease inhibitors (Calbiochem, Burlington, MA) followed by ultracentrifugation at 100,000g for 45 min ...
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bioRxiv - Molecular Biology 2019Quote: ... Transformants were regenerated and selected on regeneration medium containing 100 µg/ml of hygromycin B (Calbiochem, La Jolla, CA, USA) and 150 µg/ml G418 sulfate (Cellgro ...
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bioRxiv - Microbiology 2019Quote: ... Transformants were regenerated and selected on regeneration medium containing 100 µg/ml of hygromycin B (Calbiochem, La Jolla, CA, USA) and/or 150 µg/ml G418 sulfate (Cellgro ...
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bioRxiv - Cell Biology 2021Quote: ... Strains expressing both CDC11A-mCherry-NAT and CDC11B-GFP-NEO were constructed by transforming AG230 with a 7678bp fragment from PciI/NotI/PspXI digested AGB1320 and selecting on media containing 100ug/ml G418 (CalBiochem). Transformants were verified by PCR ...
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bioRxiv - Genomics 2019Quote: ... Pulverized material was thawed in glycerol containing nuclear isolation buffer to stabilize nuclear structure and then filtered through Miracloth (Calbiochem) to remove large tissue debris ...
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bioRxiv - Cell Biology 2022Quote: ... Forty-eight hours post transfection cells were removed and incubated in 250 μL/well of HEK Lysis Buffer containing protease Inhibitor Cocktail Set V (Calbiochem) before being centrifuged at 13 000 rpm for 10 min ...
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bioRxiv - Cell Biology 2023Quote: ... cells were washed 3 times with PBS-T and glass coverslips were added on cells (mitosis) or coverslips were mounted onto slides with Mowiol containing DAPI (Calbiochem).
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bioRxiv - Molecular Biology 2024Quote: ... the transfected cells were split and plated into three 10-cm dishes in culture medium containing selection antibiotics blasticidin S (15 μg/ml, Calbiochem) and hygromycin B (100 μg/ml ...
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bioRxiv - Molecular Biology 2023Quote: ... 10 mM EDTA, 1% NP-40, 1% sodium deoxycholate, 0.1% SDS, 1 mM DTT, 5 mM PMSF, and 1:100 Calbiochem Complete Protease Inhibitor Cocktails VII and V ...
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bioRxiv - Microbiology 2023Quote: ... followed by probing with an anti-mouse antibody conjugated to HRP (Calbiochem, Cat. No. 401253) for one h and washed three times with 0.05% Tween20 in 1X PBS ...
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bioRxiv - Cancer Biology 2019Quote: ... mice received 1 µg DT (322326-1, Calbiochem) diluted in 100 µl PBS through intraperitoneal (i.p. ...
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bioRxiv - Bioengineering 2020Quote: ... The light/heavy peptide mixture was incubated with 60 μL protein G agarose bead slurry (Calbiochem) conjugated to an antibody cocktail containing 12 μg 4G10 (Millipore) ...
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bioRxiv - Microbiology 2023Quote: ... The reaction mixture contains 8 μg total proteins and 4 μg nitrocefin (Calbiochem, San Diego, CA). β-Lactamase activity was determined by measuring the absorbance at 486 nm (OD486nm).
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bioRxiv - Microbiology 2021Quote: ... Anesthetized animals were intranasally infected using 30 to 50 μl of appropriately diluted viruses or PBS containing the indicated amounts of recombinant murine IFN-β (Calbiochem), universal-IFN (PBL assay science ...
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bioRxiv - Microbiology 2019Quote: ... and scraped into 100 uL ice-cold RIPA lysis buffer containing 1X protease inhibitor cocktail III (Calbiochem, EMD Millipore, Darmstadt, Germany) and 1X PhosSTOP phosphatase inhibitor cocktail (Roche ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Cells were then lysed in Pierce™ IP Lysis Buffer (87788, ThermoFischer Scientific, Rockford, USA) containing proteases (539138, Calbiochem, Darmstadt, Germany) and phosphatases inhibitors (524629 ...
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bioRxiv - Molecular Biology 2024Quote: ... cells were released from the arrest by transferring them to α-factor-free medium containing 150 μg/mL of Pronase (Calbiochem) and cultured for 30 min ...