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Citations for Calbiochem :
101 - 150 of 171 citations for Mouse Protein N terminal asparagine amidohydrolase NTAN1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... GAPDH was detected using mouse monoclonal anti-GAPDH antibody 6C5 (Calbiochem), and biotinylated proteins were detected using streptavidin-HRP (Invitrogen S911) ...
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bioRxiv - Biochemistry 2020Quote: ... The LF and MMP9 distribution was assessed by immunoblot using antibodies (anti-LF, Dako, rabbit polyclonal, 1/2500; anti-MMP9, Calbiochem, rabbit polyclonal n°444236, 1/2000). When appropriate ...
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bioRxiv - Cancer Biology 2022Quote: ... Protein A/G plus agarose suspension solution was obtained from Calbiochem (MERCK) and Dimethyl Sulfoxide (DMSO ...
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bioRxiv - Cell Biology 2021Quote: ... Mouse monoclonal antibodies against human Hsp90α (CA1023) purchased from Calbiochem (Billerica, MA) and Stressmarq Biosciences (Victoria ...
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bioRxiv - Neuroscience 2020Quote: ... unless staining involved the use of mouse anti-CC1 (1:100 Calbiochem), in which case antibodies were diluted in Tris buffered saline (TBS ...
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bioRxiv - Microbiology 2019Quote: ... and GAPDH was detected using mouse monoclonal anti-GAPDH antibody 6C5 (Calbiochem). Horseradish peroxidase (HRP ...
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bioRxiv - Cell Biology 2020Quote: ... stained with 1:600 dilution of secondary anti-mouse Alexa 647 (Calbiochem), and then washed three times ...
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bioRxiv - Cancer Biology 2023Quote: ... and incubated in primary antibodies for PCNA (mouse, 1:200, Calbiochem #PC10) and γH2AX (rabbit ...
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bioRxiv - Cell Biology 2023Quote: ... LAMP1 (rat, Developmental Studies Hybridoma Bank 1D4B) and Tubulin-□ (mouse, Calbiochem cp06) primary antibodies were diluted 1:200 and 1:500 respectively in TBS plus 1% normal donkey serum and 0.1% Triton X-100 ...
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bioRxiv - Genetics 2021Quote: ... cells were treated with 50 μM of the protein synthesis inhibitor cycloheximide (Calbiochem), or ethanol as vehicle control during 4 h or 8 h ...
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bioRxiv - Bioengineering 2021Quote: ... pH 7.4] and incubated with 60 μL protein G agarose bead slurry (Calbiochem) conjugated to an antibody cocktail containing 24 μg 4G10 (Millipore ...
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bioRxiv - Cell Biology 2021Quote: 20 mg of mitochondria were lysed with digitonin (Calbiochem; 6.6 g/g protein) for 15 min on ice with occasional vortexing and cleared from insoluble material for 20 min at 20,000 × g at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... 10 µM of indicated proteins were mixed with 100 µM nitrocefin (Calbiochem, Sigma) in a reaction buffer containing EstG storage buffer (50 mM HEPES-NaOH pH 7.2 ...
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bioRxiv - Cell Biology 2022Quote: ... followed by the addition 40μl of Protein A or G–Sepharose beads (Calbiochem) and incubated for 4h at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... mouse monoclonal IgG2a anti-SV40-LTag cross-reacting with BKPyV LTag (PAb416, Calbiochem), mouse monoclonal IgG1 anti-BKPyV Vp1 (MAB3204-M19 ...
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bioRxiv - Molecular Biology 2020Quote: ... Plates were then washed to remove free protein prior to addition of nitrocefin (Calbiochem) for 1 hour at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... for 1 hr followed by 1:500 dilution of anti-mouse Alexa-594 (Calbiochem) for 45 min at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... Mouse sections were mounted on glass slides and coverslipped with Mowiol (Calbiochem, Cat. 475904). Images were captured using an Olympus BX41 microscope with an Olympus camera DP-70 (Olympus Denmark A/S).
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bioRxiv - Cell Biology 2024Quote: ... anti-USP14 and HSC70 anti-mouse IgM-HRP conjugate (Calbiochem, Cat# 401225,1:10,000 dilution). Image Studio software (Licor ...
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bioRxiv - Cell Biology 2020Quote: ... Wnt-surrogate55 (0.15 nM, U-Protein Expression) and Rho kinase inhibitor (10 μM, Calbiochem Y27632) was added to the media whenever cells were at a single-cell stage to help recovery ...
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bioRxiv - Cancer Biology 2022Quote: ... Immunodetection of RAS proteins was carried out with pan-RAS (Ab-3; Calbiochem; 1:1,000) antibodies ...
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bioRxiv - Cell Biology 2022Quote: ... Mouse pancreatic islets were lysed with radioimmunoprecipitation assay buffer containing protease and phosphatase inhibitors (Calbiochem), and insoluble material was removed by centrifugation ...
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bioRxiv - Microbiology 2023Quote: ... followed by probing with an anti-mouse antibody conjugated to HRP (Calbiochem, Cat. No. 401253) for one h and washed three times with 0.05% Tween20 in 1X PBS ...
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bioRxiv - Bioengineering 2020Quote: ... The light/heavy peptide mixture was incubated with 60 μL protein G agarose bead slurry (Calbiochem) conjugated to an antibody cocktail containing 12 μg 4G10 (Millipore) ...
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bioRxiv - Microbiology 2023Quote: ... The reaction mixture contains 8 μg total proteins and 4 μg nitrocefin (Calbiochem, San Diego, CA). β-Lactamase activity was determined by measuring the absorbance at 486 nm (OD486nm).
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bioRxiv - Microbiology 2022Quote: ... and GAPDH (glyceraldehyde-3-phosphate dehydrogenase) was detected using mouse monoclonal anti-GAPDH antibody 6C5 (Calbiochem). Horseradish peroxidase (HRP ...
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bioRxiv - Cell Biology 2024Quote: ... Membranes were incubated overnight at 4°C,with mouse anti-PGC-1α (Calbiochem, KP9803, 1:1000), mouse anti-PINK1 (Santa Cruz Biotechnology ...
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bioRxiv - Cancer Biology 2020Quote: Whole cell protein lysates were prepared in RIPA buffer supplemented with Phosphatase Inhibitor Cocktail Set-II (Calbiochem) and protease inhibitor (VWR ...
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bioRxiv - Neuroscience 2023Quote: ... after which the gels was washed with water and all protein was stained using RAPIDstain (Calbiochem, 553215) for 1 hour ...
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bioRxiv - Neuroscience 2022Quote: ... the retinas were treated with the primary antibodies (1000x + 1000x mouse SMI31 + SMI32 = SMI312, NE1022 / NE1023 - Calbiochem; 1000x rabbit Caspase3 ...
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bioRxiv - Molecular Biology 2023Quote: ... The lower half of the membrane was probed with mouse anti-GAPDH (Calbiochem clone 6C5 CB1001-500UG) at 1:10,000 while the upper half was probed with mouse anti-FLAG (Sigma-Aldrich/Millipore F1804-1MG ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: Brain tissue was homogenized in RIPA buffer supplemented with protease and protein phosphatase inhibitors (Calbiochem, La Jolla, CA) and subjected to SDS-PAGE followed by transferring to PVDF membranes (Millipore ...
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bioRxiv - Physiology 2023Quote: Total protein extracts were obtained from microalgal cells in exponential phase of growth (e.g. 4 days) in 50 mM Tris buffer pH 8.0 supplemented with protein inhibitor cocktail (539131, Calbiochem). Free-living and symbiotic microalgae were disrupted by bead beating with a Precellys device (Bertin Technologies ...
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bioRxiv - Molecular Biology 2019Quote: The ProteoExtract® Subcellular Proteome Extraction Kit (Calbiochem) was used to generate lysates from the cytosolic ...
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bioRxiv - Cell Biology 2023Quote: ... mouse organs were homogenized in 2% SDS lysis buffer containing protease inhibitor mixture III (# 535140, Calbiochem, Billerica, MA) and phosphatase inhibitors (#P2850 ...
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bioRxiv - Molecular Biology 2020Quote: ... or with polyclonal anti-Sir3 antibodies and immune complexes were collected with Protein G/A beads (Calbiochem, EMD Biosciences). Immunoprecipitated and input DNA were purified using Chelex 100 (Bio-Rad ...
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bioRxiv - Immunology 2021Quote: ... under the CD11c/Itgax promoter upon intraperitoneal (i.p.) injection of 4 ng/g of mouse body weight of diphtheria toxin (DT, Calbiochem) 12 hrs prior to the targeted CD11c+ cell depletion time as indicated in the relevant figure legends ...
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bioRxiv - Plant Biology 2021Quote: ... The chromatin complexes were immunoprecipitated with anti-GFP antibody (ab290) and an anti-myc mouse antibody (mAb 9E10, Calbiochem) with a concentration of 2 μg / sample and incubated in ChIP dilution buffer with 20 μL of Dynabeads Protein A (Thermofisher ...
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bioRxiv - Immunology 2021Quote: CCR2+ and CD11c+ cells were depleted from diphtheria toxin receptor (DTR)-expressing mice upon intraperitoneal (i.p.) injection of 10 ng/g of mouse body weight of diphtheria toxin (DT, Calbiochem) 12 hrs prior to Lm challenge infection.
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bioRxiv - Microbiology 2019Quote: ... Lysates were cleared by centrifugation and precipitated with anti-HA antibody (clone HA-7; Bioshop) using agarose protein G beads (Calbiochem) as previously described25 ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins were extracted using an 8M urea lysis buffer (8M urea, 50 mM Tris-HCl, 75 mM NaCl, 1x Calbiochem protease inhibitor ...
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bioRxiv - Plant Biology 2019Quote: ... After 30 min incubation on a rotator at 4°C the protein samples were centrifuged at 18,000xg for 30 min to remove plant debris and the protein extract was filtered through Miracloth filter paper (Calbiochem Co.). Next ...
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bioRxiv - Molecular Biology 2021Quote: ... The expression of recombinant PGC-1α proteins was induced for 3 h at 37°C with 0.4 mM isopropyl-β-D-thiogalactoside (IPTG, Calbiochem) in Terrific Broth and purified by metal ion affinity chromatography (IMAC ...
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bioRxiv - Biochemistry 2022Quote: ... lysed in M-PER mammalian protein extraction reagent (Thermo-Fisher) with 1/1000 of the EDTA-free protease inhibitor cocktail Set III (Calbiochem), and centrifuged for 30 min at 15,000 × g at 4°C ...
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bioRxiv - Cell Biology 2019Quote: ... and incubated overnight at 4 °C with mouse monoclonal antibodies against type I collagen (20 μg/mL, CP-17, Calbiochem), type II collagen (0.4 μg/mL ...
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bioRxiv - Microbiology 2020Quote: ... Samples were incubated at 30°C for 1 h prior to SDS-PAGE and Western blotting with a mouse mono- and polyubiquitinated conjugate (Ubiquigent) or rabbit CHIP antibody (Calbiochem) with HRP-conjugated anti-mouse (GE Healthcare ...
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bioRxiv - Immunology 2019Quote: ... Sections were then incubated overnight in (4°C) with anti-c-ErbB2/c-Neu (Ab-3) mouse mAb (3B5) (1:500, Calbiochem). After washing with PBS ...
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bioRxiv - Immunology 2019Quote: ... bovine serum albumin in 0.1 M phosphate buffer for 1 h at room temperature and incubated overnight (4°C) with anti-c-ErbB2/c-Neu (Ab-3) mouse mAb (3B5) (1:500, Calbiochem) or anti-CD3 (1:500 ...
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bioRxiv - Cell Biology 2021Quote: Commercially available antibodies against the following proteins were used for Western blotting in the indicated dilutions: BRCA2 (OP95, Calbiochem, 1:1000), RAD51 (70-001 ...
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bioRxiv - Genomics 2019Quote: ... and SV40 large T antigen of the BK virus (Affinity purified and agarose conjugated IgG2A mouse monoclonal antibody recognizing the 94kDa SV40 large T antigen; PAb416, Cat. No. DPO2, Calbiochem, USA) respectively.