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Citations for Calbiochem :
101 - 148 of 148 citations for Mouse Glycerol kinase GK ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... Mouse sections were mounted on glass slides and coverslipped with Mowiol (Calbiochem, Cat. 475904). Images were captured using an Olympus BX41 microscope with an Olympus camera DP-70 (Olympus Denmark A/S).
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bioRxiv - Cell Biology 2024Quote: ... anti-USP14 and HSC70 anti-mouse IgM-HRP conjugate (Calbiochem, Cat# 401225,1:10,000 dilution). Image Studio software (Licor ...
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bioRxiv - Cell Biology 2022Quote: ... Mouse pancreatic islets were lysed with radioimmunoprecipitation assay buffer containing protease and phosphatase inhibitors (Calbiochem), and insoluble material was removed by centrifugation ...
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bioRxiv - Microbiology 2023Quote: ... followed by probing with an anti-mouse antibody conjugated to HRP (Calbiochem, Cat. No. 401253) for one h and washed three times with 0.05% Tween20 in 1X PBS ...
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bioRxiv - Microbiology 2022Quote: ... and GAPDH (glyceraldehyde-3-phosphate dehydrogenase) was detected using mouse monoclonal anti-GAPDH antibody 6C5 (Calbiochem). Horseradish peroxidase (HRP ...
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bioRxiv - Cell Biology 2024Quote: ... Membranes were incubated overnight at 4°C,with mouse anti-PGC-1α (Calbiochem, KP9803, 1:1000), mouse anti-PINK1 (Santa Cruz Biotechnology ...
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bioRxiv - Cell Biology 2022Quote: ... a proteoExtract protein precipitation kit (Calbiochem, #539180) was used followed by mass spectrometry analysis (Taplin Biological Mass Spectrometry Facility ...
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bioRxiv - Neuroscience 2021Quote: ... they were incubated for 2 h in secondary antibody (anti-mouse fluorescein conjugated, Calbiochem cat n. 401234) 1:100 in BSA 1% + sheep serum 2% in PBT ...
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bioRxiv - Neuroscience 2022Quote: ... the retinas were treated with the primary antibodies (1000x + 1000x mouse SMI31 + SMI32 = SMI312, NE1022 / NE1023 - Calbiochem; 1000x rabbit Caspase3 ...
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bioRxiv - Molecular Biology 2023Quote: ... The lower half of the membrane was probed with mouse anti-GAPDH (Calbiochem clone 6C5 CB1001-500UG) at 1:10,000 while the upper half was probed with mouse anti-FLAG (Sigma-Aldrich/Millipore F1804-1MG ...
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bioRxiv - Molecular Biology 2019Quote: The ProteoExtract® Subcellular Proteome Extraction Kit (Calbiochem) was used to generate lysates from the cytosolic ...
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bioRxiv - Cell Biology 2023Quote: ... mouse organs were homogenized in 2% SDS lysis buffer containing protease inhibitor mixture III (# 535140, Calbiochem, Billerica, MA) and phosphatase inhibitors (#P2850 ...
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bioRxiv - Immunology 2021Quote: ... under the CD11c/Itgax promoter upon intraperitoneal (i.p.) injection of 4 ng/g of mouse body weight of diphtheria toxin (DT, Calbiochem) 12 hrs prior to the targeted CD11c+ cell depletion time as indicated in the relevant figure legends ...
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bioRxiv - Neuroscience 2019Quote: ... Fyn was IPed with 1µg Fyn antibody (mouse monoclonal antibody [mAb], sc-434, SantaCruz) and 10 µL Protein G plus-agarose (CalBiochem). Washed Protein G bead pellets were resuspended in 2X Laemmli buffer and subjected to Western blot analysis ...
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bioRxiv - Plant Biology 2021Quote: ... The chromatin complexes were immunoprecipitated with anti-GFP antibody (ab290) and an anti-myc mouse antibody (mAb 9E10, Calbiochem) with a concentration of 2 μg / sample and incubated in ChIP dilution buffer with 20 μL of Dynabeads Protein A (Thermofisher ...
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bioRxiv - Immunology 2021Quote: CCR2+ and CD11c+ cells were depleted from diphtheria toxin receptor (DTR)-expressing mice upon intraperitoneal (i.p.) injection of 10 ng/g of mouse body weight of diphtheria toxin (DT, Calbiochem) 12 hrs prior to Lm challenge infection.
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bioRxiv - Immunology 2021Quote: ... then precipitated with the ProteoExtract Protein Precipitation Kit (Calbiochem). Equal amounts of protein for each set of samples (e.g ...
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bioRxiv - Immunology 2022Quote: ... prior to protein precipitation (Calbiochem Proteo-Extract precipitation kit). Precipitants were resuspended in 1% SDS and quantified by BCA assay for normalization ...
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bioRxiv - Biochemistry 2024Quote: ... Proteins were precipitated out using ProteoExtract precipitation kit (Calbiochem) and dissolved in 1X Laemmli buffer ...
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bioRxiv - Cell Biology 2019Quote: ... and incubated overnight at 4 °C with mouse monoclonal antibodies against type I collagen (20 μg/mL, CP-17, Calbiochem), type II collagen (0.4 μg/mL ...
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bioRxiv - Microbiology 2020Quote: ... Samples were incubated at 30°C for 1 h prior to SDS-PAGE and Western blotting with a mouse mono- and polyubiquitinated conjugate (Ubiquigent) or rabbit CHIP antibody (Calbiochem) with HRP-conjugated anti-mouse (GE Healthcare ...
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bioRxiv - Immunology 2019Quote: ... Sections were then incubated overnight in (4°C) with anti-c-ErbB2/c-Neu (Ab-3) mouse mAb (3B5) (1:500, Calbiochem). After washing with PBS ...
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bioRxiv - Immunology 2019Quote: ... bovine serum albumin in 0.1 M phosphate buffer for 1 h at room temperature and incubated overnight (4°C) with anti-c-ErbB2/c-Neu (Ab-3) mouse mAb (3B5) (1:500, Calbiochem) or anti-CD3 (1:500 ...
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bioRxiv - Immunology 2021Quote: ... Proteins were precipitated with the ProteoExtract Protein Precipitation Kit (Calbiochem) and quantified by Coomassie (Bradford ...
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bioRxiv - Genomics 2019Quote: ... and SV40 large T antigen of the BK virus (Affinity purified and agarose conjugated IgG2A mouse monoclonal antibody recognizing the 94kDa SV40 large T antigen; PAb416, Cat. No. DPO2, Calbiochem, USA) respectively.
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bioRxiv - Genetics 2023Quote: ... Raw values were normalized by geometric mean and used for subsequent housekeeping normalization (α-tubulin values; 1:10,000 mouse anti-tubulin; Calbiochem CP06) from the same blot.
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bioRxiv - Cell Biology 2022Quote: ... wild-type mouse testes (n=3 per IP assay) were lysed in 1% NP-40 (PBS) supplemented with protease inhibitor cocktail (Calbiochem, 539134). KATNA1 ...
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bioRxiv - Neuroscience 2021Quote: Calcineurin activity was assessed using Calcineurin activity assay kit (#20700, Calbiochem). Hippocampus tissue of 8 weeks old animals was homogenised in the lysis buffer (25 mM Tris-HCl pH 7.5 ...
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bioRxiv - Neuroscience 2024Quote: ... TdT Colorimetric FragEL DNA Fragmentation Detection Kit (QIA33 TdT FragEL; Calbiochem) was used for TUNEL staining ...
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bioRxiv - Neuroscience 2020Quote: Mice were submitted to 24 h of fasting and then received ip injections with either saline (0.9% NaCl) or mouse recombinant leptin (5 mg/kg body weight, Calbiochem, Billerica, MA, USA) immediately before the dark cycle ...
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bioRxiv - Neuroscience 2020Quote: Mice were submitted to 12 h of fasting and then received ip injections with mouse leptin (5 mg/kg body weight, Calbiochem, Billerica, MA, USA). After 1 h ...
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bioRxiv - Evolutionary Biology 2019Quote: ... we used the Superoxide Dismutase Assay Kit II (catalogue no. 574601, Calbiochem, supplied by Merck Pty Ltd. ...
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bioRxiv - Systems Biology 2021Quote: ... The proteins were then precipitated using Proteoextract protein precipitation kit (Calbiochem; cat # 539180) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... the cytoskeleton) were extracted using the “ProteoExtract Subcellular Proteome Extraction kit” (Calbiochem/ MERCK) according to the supplier’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: We used a non-specific Protease Activity Assay Kit (Calbiochem, cat. no. 539125) for 96-well plates as previously described (Schulte et al. ...
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bioRxiv - Pathology 2022Quote: ... and a mouse monoclonal antibody raised against Simian Virus-40 (SV-40, Macaca mulatta polyomavirus-1, Betapolyomavirus macacae) LT antigen (CalBiochem®, Clone PAb416, Sigma-Aldrich) was used as primary antibody at a 1:200 dilution ...
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bioRxiv - Developmental Biology 2021Quote: ... All primary/secondary antibodies were diluted in SignalBoost™ Immunoreaction Enhancer Kit (407207–1KIT, Calbiochem). After three washes in PBS ...
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bioRxiv - Neuroscience 2020Quote: TUNEL staining was performed using the FragEL DNA Fragmentation Detection Kit from Calbiochem (EMD Millipore). 10-day old adult fly brains were dissected in PBST and then fixed in 4% paraformaldehyde for 20 minutes on ice ...
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bioRxiv - Biochemistry 2020Quote: ... all fractions were individually precipitated using the Proteoextract Protein Precipitation Kit (EMD Milipore, Calbiochem, 539180-1KIT). For each 600 µL fraction ...
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bioRxiv - Biophysics 2020Quote: ... The cells were washed three times in the washing buffer 1X of OxyDNA Assay Kit (500095, Calbiochem) and incubated overnight at 37°C in FITC-conjugated anti 8-oxoguanine (500095 ...
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bioRxiv - Developmental Biology 2021Quote: Native membrane proteins were extracted from transfected cells using the ProteoExtract® Native Membrane Protein Extraction Kit (Calbiochem). Proteins from whole cells and from the membrane and soluble fractions were separated on a 12% SDS-PAGE protein gel ...
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bioRxiv - Neuroscience 2021Quote: Cytoplasmic and nuclear fractions were prepared from cell lysates using a ProteoExtract® Subcellular Proteome Extraction Kit (Calbiochem) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2022Quote: ... an aliquot of the cushion supernatant and eluate were precipitated using the ProteoExtract protein precipitation kit (Calbiochem 539180) per manufacturer protocol and redissolved in 2X Laemmli buffer (Fisher 50-196-784) ...
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bioRxiv - Developmental Biology 2022Quote: ... we performed terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay according to the manufacturer’s instructions (FragEL™ DNA Fragmentation Detection Kit, Colorimetric-Klenow Enzyme, Calbiochem). Briefly ...
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bioRxiv - Developmental Biology 2020Quote: Apoptotic chondrocytes in frontal knee joint sections were assessed for Terminal deoxynucleotidyltransferase-mediated dUTP Nick End Labeling (TUNEL) using DNA Fragmentation Detection Kit (QIA33-1KT, Calbiochem) and following manufacturer’s instructions.
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bioRxiv - Neuroscience 2022Quote: Cultures were rinsed twice with PBS and cell membrane extracts were collected according to the method of ProteoExtract Kit (Calbiochem). Samples were heated with equal volumes of SDS sample buffer (Novex ...
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bioRxiv - Biophysics 2020Quote: ... Cells were stained with Annexin-V-FITC and propidium iodide as indicated in the manual of the annexin V-FITC detection kit (CalbioChem, Darmstadt, Germany) and fluorescence was measured using a FACS Calibur flow cytometer (Becton-Dickinson ...
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bioRxiv - Physiology 2021Quote: Sample preparation - In-gel digests were performed for each excised sample using the ProteoExtract All-in-One Trypsin Digestion Kit (Cat. No. 650212; Calbiochem, EMD Millipore, Billerica, MA) according to manufacturer’s instructions ...