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Citations for Calbiochem :
251 - 300 of 316 citations for Monocyclohexyl Phthalate Unlabeled 100 Ug Ml In Mtbe since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2019Quote: ... cultured in R10 for several days and activated with 5 μg/ml phytohaemagglutinin (PHA-L, Calbiochem, Millipore Sigma ...
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bioRxiv - Zoology 2022Quote: ... The inoculum was then removed and 1 ml of 1 % carboxymethyl cellulose (CMC) (Aquacide II, Calbiochem) and 2% FBS diluted in RPMI medium was added ...
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bioRxiv - Neuroscience 2021Quote: ... the brain was dissected and homogenized in RIPA extraction buffer (10mM HEPES/NaOH pH 7.4, 150mM NaCl, 1% Triton X-100, 1% SDS) with protease inhibitors (Calbiochem Protease Inhibitor Set V EDTA-free). Western blot analysis was performed with Bio-Rad wet transfer system and PVDF membrane (Millipore) ...
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bioRxiv - Neuroscience 2020Quote: ... Bacteria were resuspended in (50mM HEPES, 250mM NaCl, 10% glycerol plus 1% Triton X-100, supplemented with protease inhibitors (Merck, Calbiochem set III, Cat. No. 539134) and subsequently sonicated ...
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bioRxiv - Cell Biology 2022Quote: ... or kinase domain ERK3 in kinase buffer (250 mM HEPES pH 7.5, 500 mM NaCl, 25 mM MgCl2, supplemented with protease inhibitor cocktail Set I-Calbiochem 1:100 (Cat# 539131, Merck Millipore) and phosphatase inhibitors ...
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bioRxiv - Microbiology 2019Quote: ... by electroporation (310 V, 950 μF) (58).Transfected parasites were selected over 2.5 µg/ml blasticidin (Calbiochem) and 5 µM trimethoprim (TMP ...
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bioRxiv - Developmental Biology 2021Quote: ... VEGF Receptor Tyrosine Kinase Inhibitor II (VEGFR-TKI II; Calbiochem/EMD Millipore #676481-5MG, 1.8 μg/mL) or equivalent amount of DMSO vehicle was added to media.
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bioRxiv - Developmental Biology 2021Quote: ... 1.8 μg/mL VEGF Receptor Tyrosine Kinase Inhibitor II (VEGFR-TKI II; Calbiochem/EMD Millipore #676481-5MG) dissolved in DMSO was used to disrupt blood vessels57 and 100 μM γ-secretase inhibitor IX (DAPT ...
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bioRxiv - Molecular Biology 2020Quote: ... Pellets were resuspended in 300 µl of DMEM 2% FBS 1 µg/mL propidium iodide (Calbiochem, 537060) and filtered through a 40-µm cell strainer (BD Falcon ...
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bioRxiv - Cell Biology 2019Quote: ... DNA was stained with 0.5 µg/ml DAPI (Serva) and coverslips mounted with Mowiol mounting medium (Calbiochem). The individual electroporation of HeLa cell with either the CFP-BUBR11-571 constructs or MAD2TAMRA was performed using an EP concentration of respectively 10 µM and 5 µM ...
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bioRxiv - Pathology 2020Quote: ... sclerotiorum transformants were cultured on PDA amended with 80 μg/ml hygromycin B (Calbiochem, San Diego, CA) to stabilize the transformants ...
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bioRxiv - Microbiology 2020Quote: ... Sumitomo Bakelite) were coated with 0.1 ml of 10 nM C1 Inh (Complement C1 Inhibitor, Human; Calbiochem) at 4°C overnight ...
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bioRxiv - Biochemistry 2021Quote: ... whereupon the final two wash steps were replaced with a single wash in 1.0 ml of 5 mM sodium phosphate buffer (pH 7.4) containing 0.1% (w/v) sarkosyl (Calbiochem) and 0.3% (w/v ...
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bioRxiv - Microbiology 2021Quote: ... The conidiospores were washed by 5 mL distilled water and filtrated through two layers of Miracloth (Calbiochem, Germany). The yield of spores was measured by a hemocytometer ...
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bioRxiv - Molecular Biology 2021Quote: ... The macrophage cells were induced with 10 ng/ml Escherichia coli O111:B4 LPS (Calbiochem, La Jolla, CA) for activation unless specified otherwise ...
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bioRxiv - Developmental Biology 2019Quote: ... flies were shifted to cornmeal molasses food lacking Tegosept and supplemented with 10 µg/mL chloramphenicol (Calbiochem 220551).
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bioRxiv - Molecular Biology 2020Quote: WT and endogenous TcZC3H12-HA expressing epimastigotes were incubated with 10 μg/mL proteasome inhibitor (MG-132 - Calbiochem) for one hour at 28° C ...
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bioRxiv - Neuroscience 2021Quote: ... or P5–P6 DRG rat neurons were diluted to 35,000 cells/ml in Sato’s media and treated with either 1 mM dbcAMP (Calbiochem), mixed population or recombinant Histones (1-20µg/ml ...
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bioRxiv - Genetics 2021Quote: ... https://www.oeaw.ac.at/gmi/research/research-groups/ortrun-mittelsten-scheid/resources/) with or without 20 µg/ml hygromycin B (Calbiochem) and grown for 14 d under long day (LD ...
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bioRxiv - Immunology 2022Quote: ... B16-gp3327 and B16-OVA were cultured in complete DMEM GlutaMAX™-I with 100μg/ml G418 (Calbiochem). P51129 ...
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bioRxiv - Cancer Biology 2021Quote: ... and 2 mM glutamine for 24 h prior to treatment with either TGF-β (1 ng/mL, Millipore Calbiochem) or 10 uM TGF-β inhibitors (SB431542 ...
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Proteomics of broad deubiquitylase inhibition unmasks redundant enzyme function to reveal substratesbioRxiv - Biochemistry 2020Quote: Interphase extract was prepared as previously described(Murray AW 1991) but using 2 μg/ml calcium ionophore (A23187, Calbiochem) for egg activation ...
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bioRxiv - Microbiology 2019Quote: ... BCBL-1 B cells were treated with vehicle (untreated) or stimulated with 20 ng/ml TPA (in DMSO) and Sodium Butyrate (NaB) 0.3 mM (Calbiochem) (in water ...
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bioRxiv - Physiology 2021Quote: ... cells were treated with poly(I:C) (1.5 μg/mL for 4 hrs) and a combination of proteasome inhibitor (Bortezomib, 2 μM, Sigma/Calbiochem 5043140001) and prolyl endopeptidase inhibitors (Pramiracetam 10 uM ...
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bioRxiv - Biochemistry 2021Quote: ... cells were trypsinized and seeded for selection in complete media supplemented with 4 μg/mL puromycin (Calbiochem, Merck Millipore). All cell lines were passaged 3 times before use and kept in culture supplemented with 2 μg/mL puromycin and/or stored in liquid nitrogen.
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bioRxiv - Cell Biology 2022Quote: ... and at 48 hpi cells transduced with Wasabi-pIPFCW2 were selected with 3-4 mg/ml puromycin (Calbiochem, 540411) and sorted for mid-range expression of Wasabi.
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bioRxiv - Molecular Biology 2022Quote: ... The cell pellet was lysed at 0.5 × 109 cells/ml in ice-cold lysis buffer (0.1 mM TLCK, 1 μg/ml Leupeptin, 1x Phosphatase Inhibitor Cocktail II tablet (Calbiochem), 1 mM PMSF ...
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bioRxiv - Molecular Biology 2022Quote: ... Chronic low-dose inflammation was mimicked in 3T3-L1 adipocytes by incubation with 2 ng/mL TNFα (TNF; Calbiochem) for 4 d ...
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bioRxiv - Cancer Biology 2022Quote: ... The viability of the cells was then evaluated by incubating at 37 °C for 2.5 h with 200 mg/ml MTT (Calbiochem). The MTT solution was removed afterwards ...
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bioRxiv - Cancer Biology 2021Quote: ... and the epithelial progenitors were isolated by mincing and enzymatic digestion using collagenase type II (0.5mg/mL) (Calbiochem, # 234155) (2.5 h ...
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bioRxiv - Immunology 2022Quote: ... and 1 × 106 cells were treated with rapamycin (10 ng/ml, 30 min, 37°C; Calbiochem, San Diego, CA) prior to treatment with rhGH.
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bioRxiv - Cell Biology 2023Quote: ... cells were washed 3 times for 8 min in PBS-Triton 0.01% with rocking in the dark and glass coverslips were mounted on glass slides using Mowiol containing 0.75 μg/ml DAPI (Calbiochem) and imaged with a 100x or 63x objective using Zeiss epifluorescence microscope ...
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bioRxiv - Biophysics 2023Quote: ... or FGFJI (20 ng ml−1, bFGF, R&D Systems; and 200 nM JAK inhibitor, Calbiochem, Merck Millipore, Germany). Cells were collected for mechanical characterization and RNA-Seq after 5-day exposure to the respective culture conditions (30).
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bioRxiv - Cell Biology 2019Quote: ... and incubated overnight at 4 °C with mouse monoclonal antibodies against type I collagen (20 μg/mL, CP-17, Calbiochem), type II collagen (0.4 μg/mL ...
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bioRxiv - Neuroscience 2019Quote: ... Similar results were obtained when the enzyme treatment procedure was replaced by dispase (10,000 Unit / 6-8 ml) (Calbiochem-MilliporeSigma) at 31°C for 40-60 min ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were washed twice with 3 ml PBS at 400 g for 5 minutes at 4°C and 500 uL PI staining buffer was added (1X PBS, 50 μg/mL PI (Calbiochem), 2μg/mL RNase A (Sigma ...
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bioRxiv - Systems Biology 2022Quote: ... respectively) were resuspended in 1 mL of ChIP buffer containing 5 μl of protease inhibitor cocktail (CalbioChem, cat # 539134-1) (50 mM Tris-HCl pH 8 ...
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bioRxiv - Cancer Biology 2020Quote: ... slides were counterstained with DAPI (1:50000 from 10 mg/ml stock solution, Serva Eletrophoresis) and embedded with geltol (Calbiochem). 2 representative pictures of 4 biological replicates per genotype were taken with LSM 5 Exciter (Zeiss ...
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bioRxiv - Synthetic Biology 2021Quote: ... 300 mg of twelve-day old seedlings germinated and grown on MS plates under long day conditions were treated in liquid MS medium with 20 µg/ml bleomycin (Calbiochem) for the indicated time periods or for 2 hours with 20 µg/ml bleomycin ...
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bioRxiv - Physiology 2021Quote: ... or 0.2 mL (for lung) HEPES-buffered saline plus Phosphatase Inhibitor Cocktail Set II and Protease Inhibitor Cocktail Set III (Calbiochem). Protein was measured using a Pierce™ BCA protein assay kit (Thermo Fisher ...
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bioRxiv - Cell Biology 2021Quote: ... Strains expressing both CDC11A-mCherry-NAT and CDC11B-GFP-NEO were constructed by transforming AG230 with a 7678bp fragment from PciI/NotI/PspXI digested AGB1320 and selecting on media containing 100ug/ml G418 (CalBiochem). Transformants were verified by PCR ...
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bioRxiv - Neuroscience 2022Quote: ... we gently separated the retina from sclera and RPE using fine forceps or electrically-sharpened tungsten electrodes (74) and immediately started incubation in papain solution (5 U/mL papain Calbiochem#5125 ...
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bioRxiv - Immunology 2022Quote: ... Cells were left unstimulated or stimulated for indicated time at 37 °C with PMA (10 ng/ml; Alexis) and ionomycin (1 μM; Calbiochem) or solvent (DMSO) ...
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bioRxiv - Immunology 2022Quote: ... Stable clones expressing GFP-LC3 were selected and cultured in the presence of 1000 μg/ml geneticin (G-418, Calbiochem). pEGFP-LC3 (human ...
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bioRxiv - Molecular Biology 2023Quote: ... The selection of hygromycin-resistant clones was performed on the medium with 200 µg/ml of hygromycin B (Calbiochem, #400050). The knock-out of Cul3 ...
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bioRxiv - Cell Biology 2023Quote: ... and cells were held for 16 hours in 1% Lipoprotein Deficient Serum (LDS) (Biomedical Technologies, BT907) and 25 μg/ml ALLN (Calbiochem) for 30 min prior to harvesting.
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Identification of plants functional counterparts of the metazoan Mediator of DNA Damage Checkpoint 1bioRxiv - Cell Biology 2023Quote: 300 mg of 12-14 days old WT and bcp1-4 mutant seedlings grown on vertical MS plates were transferred into liquid MS media in the presence or absence of 20 µg/ml bleomycin (Calbiochem). Following vacuum infiltration for 2 min ...
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bioRxiv - Molecular Biology 2024Quote: ... the transfected cells were split and plated into three 10-cm dishes in culture medium containing selection antibiotics blasticidin S (15 μg/ml, Calbiochem) and hygromycin B (100 μg/ml ...
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bioRxiv - Cell Biology 2019Quote: ... 2-3 × 105 single cells EpiSCs were seeded on 5 μg/10 cm2 fibronectin-coated 6-well plates in media containing (1 μl/ml) ROCK inhibitor (InSolution™ Y-276320, Cat.#688001-500UG, VWR Calbiochem). In the morning of transfection ...
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bioRxiv - Microbiology 2019Quote: ... or pronase (a mixture of endo- and exoproteases from Streptomyces griseus at a final concentration of 5, 10 or 50 μg/ml; Calbiochem, UK) for 30 min ...