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Citations for Calbiochem :
101 - 142 of 142 citations for IL 2 Human CHO since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reagent was obtained from Calbiochem (San Diego, CA, USA).
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bioRxiv - Physiology 2021Quote: ... and phosphatase inhibitors (2 μM microcystin-LR, and 1× Phosphatase-Inhibitor Sets I and II (Calbiochem, Hayward, CA). We centrifuged homogenates at 4,000 g for 15 minutes to remove debris ...
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bioRxiv - Pathology 2023Quote: ... 2% DDM) supplemented with 1:1000 (v/v) Protease Inhibitor Cocktail Set III (Calbiochem, La Jolla, CA, USA). Rat anti-HA (3F10 ...
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bioRxiv - Cell Biology 2023Quote: ... mouse organs were homogenized in 2% SDS lysis buffer containing protease inhibitor mixture III (# 535140, Calbiochem, Billerica, MA) and phosphatase inhibitors (#P2850 ...
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bioRxiv - Cancer Biology 2021Quote: ... and 2 mM glutamine for 24 h prior to treatment with either TGF-β (1 ng/mL, Millipore Calbiochem) or 10 uM TGF-β inhibitors (SB431542 ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... and the phenylacetamide compound (S)-2-(4-chlorophenyl)-3,3-dimethyl-N-(5-phenylthiazol-2-yl)butanamide (PA;Cmp58 (Wang et al., 2010)) was obtained from Calbiochem-Merck Millipore (Billerica ...
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Proteomics of broad deubiquitylase inhibition unmasks redundant enzyme function to reveal substratesbioRxiv - Biochemistry 2020Quote: Interphase extract was prepared as previously described(Murray AW 1991) but using 2 μg/ml calcium ionophore (A23187, Calbiochem) for egg activation ...
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bioRxiv - Physiology 2021Quote: ... cells were treated with poly(I:C) (1.5 μg/mL for 4 hrs) and a combination of proteasome inhibitor (Bortezomib, 2 μM, Sigma/Calbiochem 5043140001) and prolyl endopeptidase inhibitors (Pramiracetam 10 uM ...
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bioRxiv - Molecular Biology 2022Quote: ... Chronic low-dose inflammation was mimicked in 3T3-L1 adipocytes by incubation with 2 ng/mL TNFα (TNF; Calbiochem) for 4 d ...
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bioRxiv - Plant Biology 2022Quote: ... except that the resuspension of protein precipitate was in modified denaturing urea buffer (8 M urea, 2% SDS, 100 mM HEPES-KOH pH 7.4, 10 mM EDTA and protease inhibitor [Calbiochem, now Merck ...
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bioRxiv - Microbiology 2022Quote: ... two reactions with increasing dosages of the PfCKII-α-specific inhibitors 4,5,6,7-tetramethylbromobenzotriazole and 4,5,6,7-tetramethylbromo-2-azabenzimidazole (TBB) (Merck/Calbiochem CAS 17374-26-4) were performed to check for possible suppression of phosphoryl group transfer ...
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bioRxiv - Physiology 2023Quote: ... Fura-2 AM and BAPTA-AM (Cat# F1221, Molecular Probes, Eugene, OR, USA and CAS# 126150-97-8, Calbiochem San Diego ...
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bioRxiv - Biochemistry 2023Quote: ... Ubiquitin was expressed as described above except that the 2 L culture was induced with 0.4 mM dioxane-free isopropyl B-D-1-thiogalactopyranoside (IPTG) (Calbiochem) for four hrs at 37℃ ...
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bioRxiv - Cell Biology 2023Quote: The antibodies listed in Tables 1 and 2 were diluted as indicated in PBS containing 1% BSA (126575, Calbiochem).
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bioRxiv - Microbiology 2023Quote: ... Plates were then incubated at 37 °C for 2 h with different quantities (0.125 - 4 pmol) of biotinylated PfPP1c labeled with biotin-NHS (Calbiochem) in PBS-Tween 0.1% ...
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bioRxiv - Cell Biology 2023Quote: ... The homogenate was then incubated on ice for 5 minutes and filtered through 2 layers of Miracloth tissue (Calbiochem). The filtered homogenate was then centrifuged for 20 min at 480 rpm at 4°C to pellet any debris ...
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bioRxiv - Cancer Biology 2023Quote: NMDI-14 inhibitor (Ethyl 2-(((6,7-dimethyl-3-oxo-1,2,3,4-tetrahydro-2-quinoxalinyl)acetyl)amino)-4,5-dimethyl-3-thiophenecarboxylate) was purchased from Calbiochem (Cat. Nr. 530838) and reconstituted in DMSO (10mg/ml ...
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bioRxiv - Cell Biology 2023Quote: ... The antibodies (Tables 2 and 3) were diluted according to the tables in PBS containing 1% BSA (126575, Calbiochem).
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bioRxiv - Immunology 2021Quote: ... GMDCs were incubated for 2 h at 37°C with 50 µM MARK inhibitor (MARK/Par-1 Activity Inhibitor, 39621; Calbiochem), 50 nM SIK inhibitor (HG-9-91-01 ...
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bioRxiv - Immunology 2019Quote: ... Injection inoculum was prepared from an overnight liquid culture in the log-phase of growth resuspended in 2% polyvinylpyrrolidone40 (PVP40) solution (CalBiochem) as previously described (40) ...
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bioRxiv - Cell Biology 2021Quote: ... The antibodies listed in Table 2 and 3 were diluted according to the tables in PBS containing 1% BSA (126575, Calbiochem).
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bioRxiv - Cell Biology 2021Quote: ... Cells were arrested in S phase or at the G1/S boundary by single or double thymidine (2 mM, Calbiochem) treatment ...
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bioRxiv - Neuroscience 2021Quote: Pharmacological agents were used in the following concentrations and time courses: cycloheximide (Calbiochem, 0.2 μg/μl, 2, 4 or 8h), dexamethasone (Invivogen ...
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bioRxiv - Developmental Biology 2022Quote: ... Drosophila ovaries from aPKCas4 flies (prepared as previously described in the optogenetic experiments section) were cultured ex vivo for 2 hours in the presence of the ATP analog 1NA-PP1 (Calbiochem; prepared in DMSO ...
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bioRxiv - Cell Biology 2019Quote: ... Crosses were performed by random spore analysis (1, 2) followed by marker selection (Hygromycin/Kanamycin resistance, ura/leu auxotrophy, or sensitivity to 5 µM 1NM-PP1 (Calbiochem) for strains carrying the Ark1-as3 allele ...
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bioRxiv - Physiology 2021Quote: ... or 0.2 mL (for lung) HEPES-buffered saline plus Phosphatase Inhibitor Cocktail Set II and Protease Inhibitor Cocktail Set III (Calbiochem). Protein was measured using a Pierce™ BCA protein assay kit (Thermo Fisher ...
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bioRxiv - Molecular Biology 2021Quote: ... Fresh 2-day conidia from each strain were harvested from ANM plates in PBS solution and filtered through a Miracloth (Calbiochem). For each strain ...
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bioRxiv - Cell Biology 2022Quote: ... the P2Y2R antagonist AR-C118925 {5-[[5-(2,8-dimethyl-5Hdibenzo[α,d]cyclohepten-5-yl)-3,4-dihydro-2-oxo-4-thioxo-1(2H)-pyrimidinyl]methyl]-A-2H-tetrazol-5-yl-2-furan-carboxamide},and the Ca2+ probes thapsigargin and ionomycin were obtained from Calbiochem-Merck Millipore (Billerica ...
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bioRxiv - Cell Biology 2023Quote: ... Equivalent amounts of protein (1-2 mg) were incubated with 1 μg indicated antibody and 20 μL protein A/G beads (#IP05, Calbiochem) at 4 ℃ overnight ...
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bioRxiv - Cell Biology 2019Quote: ... (N-{(2S,4R)-4-(Biphenyl-2-ylmethyl-isobutyl-amino)-1-[2-(2,4-difluorobenzoyl)-benzoyl]-pyrrolidin-2-ylmethyl}8-3-(4-(2,4-dioxothiazoldin-5-ylidenemethyl)-phenyl]acrylamide (cPLA2 inhibitor, cPLA2 Inh, Calbiochem, EMD Biosciences), was used concomitantly with priming at 5 μM and MK-886 (FLAP Inh ...
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bioRxiv - Cancer Biology 2020Quote: ... plate wells were washed with PBS containing 2% FBS and incubated overnight with biotinylated HA-binding protein (3 μg/ml, Calbiochem-EMD Millipore) at 4° C (34) ...
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bioRxiv - Cancer Biology 2020Quote: ... plate wells were washed with PBS containing 2% FBS and incubated overnight with biotinylated HA-binding protein (3 μg/ml, Calbiochem-EMD Millipore) at 4° C (16) ...
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bioRxiv - Cell Biology 2020Quote: ... and all cell types were lysed with Pierce™ RIPA Buffer (ThermoScientific 89900) supplemented with 1X Phosphatase Inhibitor Cocktail Set 2 (Calbiochem 5246251) and 1X Protease Inhibitor Cocktail Set 1 (Calbiochem 539131) ...
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bioRxiv - Cell Biology 2021Quote: ... plate wells were washed with PBS containing 2% FBS and incubated overnight with biotinylated HA-binding protein (3 μg/ml, Calbiochem-EMD Millipore) at 4° C (7) ...
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bioRxiv - Biochemistry 2020Quote: ... 5-8×106 cell pellets were washed twice and resuspended in cold DPBS and incubated on ice for 10 min with 2 mg/mL digitonin (Calbiochem, #3000410-5GM). Next ...
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bioRxiv - Biochemistry 2021Quote: ... PTP1B KO Ramos were transfected with a doxycycline inducible tet-on vector and exposed to 2 μg/ml doxycycline (Calbiochem, Merck, Darmstadt, Germany) 24 to 48 h before the experiment ...
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bioRxiv - Cancer Biology 2020Quote: Dissected CP specimens were dissociated using forceps under a stereoscope followed by enzymatic digestion at 37°C for 20 minutes with pronase (2 mg/ml, 537088, Calbiochem, San Diego, CA) in Hank’s balanced salt solution (HBSS ...
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bioRxiv - Molecular Biology 2023Quote: ... the cells were solubilized for 1 h at 4□°C in buffer (50□mM HEPES-NaOH [pH 7.5], 300□mM NaCl, 2% (w/v) N-dodecyl β-d-maltoside (DDM, Calbiochem, San Diego, CA), protease inhibitor cocktail ...
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bioRxiv - Cancer Biology 2023Quote: ... hatched zebrafish embryos were engrafted via the Duct of Cuvier with 1×105 cells/µL tumor cells resuspended in 2% polyvinypyrrolidone 40 (PVP-40, Calbiochem, San Diego, USA) using borosilicate glass capillary needles (1mm O.D ...
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bioRxiv - Molecular Biology 2023Quote: ... the mice were anaesthetized using 2% isoflurane and given a retro-orbital injection of 21.75 mg kg−1 body weight puromycin (Calbiochem, San Diego, CA, USA) in saline to measure muscle protein synthesis73.
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bioRxiv - Molecular Biology 2021Quote: To test for the EV nature of labelled objects detergent controls were performed by adding a sample volume of a 2% (w/v) NP-40 solution (Calbiochem, San Diego, CA, USA) to the samples.
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bioRxiv - Neuroscience 2020Quote: ... (Cat. N° 487910) and 3-amino,4-aminomethyl-2’,7’-difluorofluorescein (DAF-FM) (Cat. N° 251515) were obtained from Calbiochem (San Diego, CA, USA). EZ-link HPDP-Biotin (Cat ...