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Citations for Calbiochem :
201 - 250 of 256 citations for IL 10 Mouse since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2019Quote: ... flies were shifted to cornmeal molasses food lacking Tegosept and supplemented with 10 µg/mL chloramphenicol (Calbiochem 220551).
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bioRxiv - Molecular Biology 2020Quote: WT and endogenous TcZC3H12-HA expressing epimastigotes were incubated with 10 μg/mL proteasome inhibitor (MG-132 - Calbiochem) for one hour at 28° C ...
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bioRxiv - Molecular Biology 2022Quote: ... Samples were incubated for 1 h with the ADAM17 inhibitor Batimastat (BB-94) at 10 μM (196440, Calbiochem), with the selective ADAM10 inhibitor GI254023X (GI ...
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bioRxiv - Neuroscience 2023Quote: ... After processing sections were mounted on microscope slides with Tris-glycerol supplemented with 10% Mowiol (Merck Millipore Calbiochem).
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bioRxiv - Molecular Biology 2024Quote: ... The pellet was suspended in 2 ml of lysis/binding buffer (25 mM HEPES/KOH, pH 7.6, 10 % glycerol, 150 mM KCl, 1 mM dithiothreitol, 0.1 % Elugent (Calbiochem), loaded into SHREDDER tubes (Pressure Biosciences Inc ...
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bioRxiv - Neuroscience 2020Quote: Mice were submitted to 24 h of fasting and then received ip injections with either saline (0.9% NaCl) or mouse recombinant leptin (5 mg/kg body weight, Calbiochem, Billerica, MA, USA) immediately before the dark cycle ...
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bioRxiv - Cancer Biology 2021Quote: Cell pellets were sonicated in ice-cold lysis buffer (containing 20 mM Tris-HCl, pH 7.4, 5 mM EDTA, 10 mM Na4P2O7 (Calbiochem), 100 mM NaF ...
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bioRxiv - Genetics 2021Quote: 48 hpf zebrafish larvae were treated for 24 hours with 10 µM and 50 µM ATR Inhibitor IV (Calbiochem) or DMSO as negative control ...
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bioRxiv - Plant Biology 2020Quote: ... Homogenate was centrifuged at 10,000 x g at 4°C for 10 minutes and filtered through two layers of Miracloth (Calbiochem) before centrifugation at 40,000 x g for one hour at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... In brief, lysis buffer (8 M urea, 1% Triton-100, 10 mM dithiothreitol, and 1% Protease Inhibitor Cocktail (Calbiochem) was used to extract the total protein ...
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bioRxiv - Cell Biology 2021Quote: ... Slides were then washed two times for 10 min in PBST before mounting using Mowiol (30% wt/vol glycerol, 3.87 mM Mowiol [Calbiochem, 475904] ...
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bioRxiv - Cell Biology 2021Quote: ... 50 μg of the total RNA was incubated for 1 h at 4 °C with 10 μl of the anti-2,2,7-trimethylguanosine-antibody (Calbiochem Milipore) coupled to sepharose beads (Fig ...
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bioRxiv - Cell Biology 2022Quote: Cells were seeded in 6 well plates and arrest in G2 for 20 h with 10 μM RO3306 (Calbiochem) addition ...
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bioRxiv - Immunology 2022Quote: ... and 1 × 106 cells were treated with rapamycin (10 ng/ml, 30 min, 37°C; Calbiochem, San Diego, CA) prior to treatment with rhGH.
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bioRxiv - Plant Biology 2022Quote: ... except that the resuspension of protein precipitate was in modified denaturing urea buffer (8 M urea, 2% SDS, 100 mM HEPES-KOH pH 7.4, 10 mM EDTA and protease inhibitor [Calbiochem, now Merck ...
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bioRxiv - Biochemistry 2023Quote: ... 0.1% DDM, 10% (v/v) ethylene glycol, 0.005% asolectin (Avanti Polar Lipid, USA) and 0.005% CHAPS (Calbiochem, Merck, Germany), and eluted by an increasing proportion of the same buffer plus 1 M NaCl ...
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bioRxiv - Neuroscience 2020Quote: Mice were submitted to 12 h of fasting and then received ip injections with mouse leptin (5 mg/kg body weight, Calbiochem, Billerica, MA, USA). After 1 h ...
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bioRxiv - Cell Biology 2020Quote: ... They were then pelleted down by centrifuging at 1000 rpm for 5 min and then washed with PBS supplemented with 10 μM MG-132 (Calbiochem). After centrifugation ...
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bioRxiv - Genetics 2021Quote: ... The larvae were individualized in each well of 96-well plates containing ATR Inhibitor IV (CAS 1232410-49-10 Calbiochem) at 10 µM or DMSO in egg water as negative control ...
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bioRxiv - Microbiology 2021Quote: Primary macrophages pre-treated with the following reagents one hour prior to infection with Mtb H37Rv: GSI (Calbiochem, 10 μM); EPZ015666 (Tocris ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were then incubated in a wash buffer consisting of 2X nuclease free SSC and 10% deionized formamide (Calbiochem 4610) for 5 minutes at room temperature ...
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bioRxiv - Molecular Biology 2019Quote: ... 0.1 mM PMSF and 0.1 μM Aprotinin) using a Dounce homogenizer (10 strokes, 400 rpm) and filtered through two layers of Mira cloth (Calbiochem). The filtrate was centrifuged at 600g at 4° C for 2 min to remove crud and unbroken cells ...
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bioRxiv - Neuroscience 2019Quote: ... Then preparations were incubated for 15 min with hybridization buffer prepared using 2x saline-sodium citrate (SSC) solution / 10 % deionized formamide (4610-OP, Calbiochem). Hybridization with Stellaris FISH probes was done in a total volume of 50 μl hybridization buffer containing 50 μg competitor tRNA from E.coli (10109541001 ...
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bioRxiv - Bioengineering 2019Quote: ... with 10% FBS media (500 ml of MEM, 2.5 g D-glucose, 100 mg NaHCO3, 50 mg transferrin (Calbiochem 616420), 50 ml FBS (heat inactivated ...
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bioRxiv - Microbiology 2021Quote: ... confluent epithelial cell monolayers were treated with the following inhibitors at concentrations previously determined (5): SP600125 (Primary target: JNK1, JNK2, JNK3; 10 μM, Calbiochem), SB203580 (Primary target ...
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bioRxiv - Cell Biology 2020Quote: ... the following inhibitors were used at the indicated concentrations for 4 h unless indicated differently in the experiments: RO-3306 at 10 μM (CDK1 inhibitor; Calbiochem), NU6140 at 10 μM (CDK2 inhibitor ...
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bioRxiv - Immunology 2021Quote: ... as a pan-caspase inhibitor or 10 µM N-acetyl-tyrosyl-valyl-alanyl-aspartyl chloromethyl ketone (Ac-YVAD-cmk, Calbiochem) as a caspase 1 inhibitor ...
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bioRxiv - Cancer Biology 2020Quote: ... slides were counterstained with DAPI (1:50000 from 10 mg/ml stock solution, Serva Eletrophoresis) and embedded with geltol (Calbiochem). 2 representative pictures of 4 biological replicates per genotype were taken with LSM 5 Exciter (Zeiss ...
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bioRxiv - Cell Biology 2021Quote: ... in PBS for 10 min and the coverslips were mounted cell side down on object glasses with Mowiol (Calbiochem, 475904) containing 50 mg/ml 1,4-diazabicyclo[2.2.2]octane (Dabco ...
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bioRxiv - Developmental Biology 2020Quote: ... was collected and transferred to new 6-well plates in the same culture medium with the addition of 10 µM of the Casp3 inhibitor (Ac-DMQD-CHO, Cat. 235421 – Calbiochem). The culture was maintained for more 20h ...
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bioRxiv - Cell Biology 2021Quote: ... the cell pellet was lysed by sonication in lysis buffer (2mM Tris–HCl pH 7.4, 0.5M NaCl, 20mM imidazole, 10% glycerol, Protease Inhibitor Cocktail Set III Calbiochem, 1:10,000) and centrifuged (10,000g ...
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bioRxiv - Immunology 2022Quote: ... Cells were left unstimulated or stimulated for indicated time at 37 °C with PMA (10 ng/ml; Alexis) and ionomycin (1 μM; Calbiochem) or solvent (DMSO) ...
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bioRxiv - Microbiology 2022Quote: Primary macrophages pre-treated with the following reagents one hour prior to infection with Mtb H37Rv: SB203580 (Calbiochem, 10 μM); SP600125 (Calbiochem ...
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bioRxiv - Molecular Biology 2022Quote: ... After 4 h the cells were starved in serum free medium overnight and then incubated for 1h with the ADAM17 inhibitor Batimastat (BB-94) at 10 μM (196440, Calbiochem), with the selective ADAM10 inhibitor GI254023X (GI ...
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bioRxiv - Cell Biology 2023Quote: ... the cells were fixed with cold methanol (10 min at −20°C) and blocked for 30 min with 3% (w/v) bovine serum albumin (Calbiochem) in 1 x PBS at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... The NPCs were differentiated into neurons in the absence of bFGF for at least 3 weeks and treated with 10 μM ROCK inhibitor (Y-27632; Calbiochem) during the first 24 h.
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bioRxiv - Neuroscience 2023Quote: ... After washing three times for 10 min in TBST the sections were mounted on glass slides using Mowiol (Calbiochem/ MerckMillipore), glass covered and stored at 4°C.
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bioRxiv - Molecular Biology 2024Quote: ... the transfected cells were split and plated into three 10-cm dishes in culture medium containing selection antibiotics blasticidin S (15 μg/ml, Calbiochem) and hygromycin B (100 μg/ml ...
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bioRxiv - Pathology 2024Quote: ... NETs formation was induced for 3 hours at 37°C and 5% CO2 by stimulation with 10 µM of PAF (Calbiochem) on primed neutrophils and 25 µM on non-primed ones ...
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bioRxiv - Microbiology 2019Quote: ... or pronase (a mixture of endo- and exoproteases from Streptomyces griseus at a final concentration of 5, 10 or 50 μg/ml; Calbiochem, UK) for 30 min ...
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bioRxiv - Neuroscience 2022Quote: ... frozen neural progenitor cells (NPCs) were thawed in the neural maintenance growth medium and supplemented with ROCK inhibitor (Y-27632; 10 mM Calbiochem, #688000). Cells were incubated in a humidified chamber at 37°C with 5% CO2 ...
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bioRxiv - Biochemistry 2021Quote: ... The sVSG depleted cell ghost pellet was resuspended in 1.5 ml of 20 mM ammonium bicarbonate and mixed with 50 µl of 10 mg/ml of freshly prepared Pronase (Calbiochem, #53702) dissolved in 5 mM calcium acetate and digested at 37 °C for 24 h ...
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bioRxiv - Neuroscience 2022Quote: ... the cells were then incubated overnight at 4°C with primary antibodies (rabbit anti-AUTS2, 1:200, Atlas Antibodies; rabbit anti-GRIA1, 1:10, Calbiochem; mouse anti-GRIA2 ...
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bioRxiv - Molecular Biology 2024Quote: ... and resuspended in 1.5 mL of 20 mM ammonium bicarbonate and mixed with 50 µl of 10 mg/mL of freshly prepared Pronase (Calbiochem, #53702) dissolved in 5 mM calcium acetate and digested at 37 °C for 24 h ...
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bioRxiv - Pathology 2022Quote: ... and a mouse monoclonal antibody raised against Simian Virus-40 (SV-40, Macaca mulatta polyomavirus-1, Betapolyomavirus macacae) LT antigen (CalBiochem®, Clone PAb416, Sigma-Aldrich) was used as primary antibody at a 1:200 dilution ...
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bioRxiv - Physiology 2019Quote: ... Individual gel slices were subjected to in-gel reduction with dithiothreitol (10 mM, 30 min, 56 °C, Merck-Calbiochem, San Diego, USA), alkylation with iodoacetamide (50 mM ...
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bioRxiv - Cancer Biology 2020Quote: ... dipped in MilliQ, and mounted in Mowiol (0.1 M tris-HCl pH 8.5, 25% glycerol, 10% Mowiol (Calbiochem, Merck Millipore, Darmstadt, Germany)) ...
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bioRxiv - Immunology 2021Quote: B cell staining was performed on frozen PBMCs after thawing in complete medium (RPMI 10% FBS, PS/G 1X) containing DNase I (Calbiochem, cod 260913) for 10 min at 37°C ...
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bioRxiv - Biochemistry 2020Quote: ... 5-8×106 cell pellets were washed twice and resuspended in cold DPBS and incubated on ice for 10 min with 2 mg/mL digitonin (Calbiochem, #3000410-5GM). Next ...
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bioRxiv - Plant Biology 2020Quote: ... The peels were then transferred into 10-ml buffer (Araújo et al., 2011) containing the enzyme CELLULYSIN cellulase from Trichoderma viride (Calbiochem, La Jolla, CA, USA) and digested for 1 h at a shaking speed of 150 rpm ...