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Citations for Calbiochem :
1 - 50 of 500 citations for HLA A*0201 WT 1 complex Protein Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2021Quote: ... blocked (200 μL, PBS 1% BSA wt/vol, Calbiochem #12659-100GM, 3 h, RT) and washed (3x ...
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bioRxiv - Immunology 2020Quote: ... blocked (200 µL, PBS 1% BSA wt/vol, Calbiochem #12659-100GM, 3 h, RT) and washed (3x ...
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bioRxiv - Biophysics 2022Quote: ... WT α-hemolysin (Calbiochem, Merck, Darmstadt, Germany) was diluted in an aqueous solution containing 8 M urea at 25 μg/mL (M = mol/L).
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bioRxiv - Cell Biology 2022Quote: ... 100 μM CK666 (to inhibit the Arp2/3 complex; Calbiochem/Sigma, 182515), 100 μM CK689 (an inactive control for CK666 ...
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bioRxiv - Microbiology 2019Quote: ... The blots were washed with PBS and incubated with primary antibodies (anti-EBV gH/gL (a rabbit polyclonal antiserum;1:200) (45) and anti-His tag antibody (OB05, Calbiochem, 1:1000) for His-tagged KSHV gL ...
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bioRxiv - Cell Biology 2023Quote: ... Equivalent amounts of protein (1-2 mg) were incubated with 1 μg indicated antibody and 20 μL protein A/G beads (#IP05, Calbiochem) at 4 ℃ overnight ...
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bioRxiv - Microbiology 2021Quote: ... 50 μL mouse monoclonal anti-6X His tagged primary antibody (Calbiochem) was added at a dilution of 1:150 in 0.5 % BSA in PBST in the respective wells and incubated for 2 hours without shaking at room temperature ...
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bioRxiv - Immunology 2020Quote: ... Rac1 inhibitor (NSC23766) and Arp-2 complex inhibitor I (CK-666) & II (CK-869) purchased from Calbiochem were used for blocking actin assembly (33).
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bioRxiv - Microbiology 2020Quote: Human HDL lipoproteins (LP3, Calbiochem) were labeled using the Cy5 monoreactive Dye pack (PA25001 ...
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bioRxiv - Molecular Biology 2020Quote: WT and endogenous TcZC3H12-HA expressing epimastigotes were incubated with 10 μg/mL proteasome inhibitor (MG-132 - Calbiochem) for one hour at 28° C ...
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Noradrenergic alpha-2A receptor activation suppresses courtship vocalization in male Japanese quail.bioRxiv - Animal Behavior and Cognition 2021Quote: ... Correct placement of the cannula was confirmed 1 day after surgery by infusing 1 μg of human angiotensin-II (Calbiochem, San Diego, CA, USA) dissolved in 0.9% NaCl ...
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bioRxiv - Cell Biology 2021Quote: ... Slides were then washed two times for 10 min in PBST before mounting using Mowiol (30% wt/vol glycerol, 3.87 mM Mowiol [Calbiochem, 475904] ...
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bioRxiv - Immunology 2019Quote: ... B cells (7.5 x 105cells/ml) were pre-treated with 100 µM of the Arp2/3 complex inhibitor CK-666 (Calbiochem, #CAS 442633-00-3) or the inactive analog CK-689 (Calbiochem ...
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bioRxiv - Cancer Biology 2022Quote: ... Immunodetection of RAS proteins was carried out with pan-RAS (Ab-3; Calbiochem; 1:1,000) antibodies ...
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bioRxiv - Biochemistry 2024Quote: Human myeloma IgA1 or IgA2 isoforms (Calbiochem), at 0.33 mg/ml were separately incubated with 1 µM of recombinant BT4244-M60L peptidase and incubated at 37°C in a water bath for 16h ...
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bioRxiv - Immunology 2021Quote: ... Proteins were precipitated with the ProteoExtract Protein Precipitation Kit (Calbiochem) and quantified by Coomassie (Bradford ...
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bioRxiv - Molecular Biology 2022Quote: ... After adding Protein G Plus Protein A agarose beads (Calbiochem, Germany), the mixture was incubated at 4°C for 1 hour ...
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bioRxiv - Biochemistry 2020Quote: ... Human C1q was obtained from Calbiochem (Burlington, MA). Human hemoglobin was obtained from Sigma-Aldrich (St ...
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bioRxiv - Animal Behavior and Cognition 2019Quote: ... Immune complexes were captured with Protein G/Protein A plus-agarose (Calbiochem) and subjected to Western blotting using PKL antibody (25 ng/ml ...
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bioRxiv - Microbiology 2021Quote: ... cathepsins B and L (CatB, CatL; human liver, Calbiochem) and α-chymotrypsin (α-CT ...
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bioRxiv - Neuroscience 2019Quote: ... Active recombinant human MMP-9 (Calbiochem, San Diego, CA) was used as a positive control ...
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bioRxiv - Microbiology 2023Quote: ... and 10 µg of human PLG-depleted fibrinogen (Calbiochem), or 2.5 µg of human vitronectin (Sigma-Aldrich) ...
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bioRxiv - Systems Biology 2021Quote: ... The proteins were then precipitated using Proteoextract protein precipitation kit (Calbiochem; cat # 539180) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2019Quote: ... and Protein G beads (CalBiochem). The beads containing Fyn were resuspended in 100 µL buffer (0.1% Triton ...
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bioRxiv - Cell Biology 2020Quote: ... Protein A agarose beads (Calbiochem) was added to the mixture and incubation was continued with gentle rocking for 3 hrs at 4 °C ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: Thrombin (human plasma, high activity, 5000 U, Calbiochem-EMD Millipore) and trypsin (porcine pancreas ...
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bioRxiv - Biochemistry 2022Quote: ... lysed in M-PER mammalian protein extraction reagent (Thermo-Fisher) with 1/1000 of the EDTA-free protease inhibitor cocktail Set III (Calbiochem), and centrifuged for 30 min at 15,000 × g at 4°C ...
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bioRxiv - Cell Biology 2021Quote: Commercially available antibodies against the following proteins were used for Western blotting in the indicated dilutions: BRCA2 (OP95, Calbiochem, 1:1000), RAD51 (70-001 ...
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bioRxiv - Immunology 2019Quote: ... All challenges were diluted with 0.5% human serum albumin (30% solution, Calbiochem) in PBS and administered in a 1mL volume ...
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bioRxiv - Cell Biology 2021Quote: ... Mouse monoclonal antibodies against human Hsp90α (CA1023) purchased from Calbiochem (Billerica, MA) and Stressmarq Biosciences (Victoria ...
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bioRxiv - Developmental Biology 2021Quote: Native membrane proteins were extracted from transfected cells using the ProteoExtract® Native Membrane Protein Extraction Kit (Calbiochem). Proteins from whole cells and from the membrane and soluble fractions were separated on a 12% SDS-PAGE protein gel ...
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bioRxiv - Physiology 2023Quote: ... Normal goat serum was used as a blocking agent and slides were incubated in biotinylated hyaluronan binding protein (HABP, 1:500, CalBiochem, Burlington, MA) for one hour ...
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bioRxiv - Cell Biology 2022Quote: ... a proteoExtract protein precipitation kit (Calbiochem, #539180) was used followed by mass spectrometry analysis (Taplin Biological Mass Spectrometry Facility ...
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bioRxiv - Molecular Biology 2023Quote: ... Fourty μl Protein A/G Agarose beads (Calbiochem) were added and the samples were pre-cleaned on a rocker-rotator at 4°C for 2 hours ...
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BCR analysis of single-sorted, putative IgE+ memory B cells in food allergy: an ephemeral existence?bioRxiv - Immunology 2019Quote: ... Cell-free supernatant samples and a serial dilution of purified human IgE (401152, Calbiochem) were incubated overnight at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... the pro-protein convertase inhibitor Decanoyl-RVKR-CMK (Calbiochem) was applied to cells at a concentration of 5 μM ...
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bioRxiv - Biochemistry 2022Quote: ... The protein extracts were first filtered through Miracloth (CalBiochem) to remove insoluble plant debris and subsequently centrifuged at 5,000 x g for 5 min at 4°C to remove cellular debris and organelles ...
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bioRxiv - Immunology 2021Quote: ... then precipitated with the ProteoExtract Protein Precipitation Kit (Calbiochem). Equal amounts of protein for each set of samples (e.g ...
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bioRxiv - Immunology 2022Quote: ... prior to protein precipitation (Calbiochem Proteo-Extract precipitation kit). Precipitants were resuspended in 1% SDS and quantified by BCA assay for normalization ...
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bioRxiv - Biochemistry 2024Quote: ... Proteins were precipitated out using ProteoExtract precipitation kit (Calbiochem) and dissolved in 1X Laemmli buffer ...
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bioRxiv - Microbiology 2019Quote: ... 20 µL protein A agarose suspension (Calbiochem/Millipore #IP02-1.5ML) was added to each and samples were rocked at 4°C for 2 hours ...
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bioRxiv - Cell Biology 2021Quote: ... and proteins were immunoprecipitated on BRCA2 antibody-conjugated (OP95, Calbiochem) Rec-protein G Sepharose 4B beads (Invitrogen ...
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bioRxiv - Neuroscience 2021Quote: ... Injections of 50 µl NGF at a concentration of 0.8 µM (NGF, human recombinant, Calbiochem, Merck, Germany) dissolved in phosphate buffer saline (PBS ...
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bioRxiv - Microbiology 2020Quote: ... Sumitomo Bakelite) were coated with 0.1 ml of 10 nM C1 Inh (Complement C1 Inhibitor, Human; Calbiochem) at 4°C overnight ...
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bioRxiv - Biochemistry 2020Quote: ... Lambda protein phosphatase and alkaline phosphatase were from Calbiochem (#539514-20KV) and Sigma-Aldrich (#P6774-2KU) ...
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bioRxiv - Cancer Biology 2022Quote: ... Purified proteins were dialyzed against 20 mm Tris/HCl (MERCK, Calbiochem, catalogue No ...
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bioRxiv - Neuroscience 2024Quote: ... and 50 µM Rho-associated protein kinase inhibitor (ROCKi; Calbiochem, USA). This media was changed every other day for 6 days ...
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bioRxiv - Cancer Biology 2022Quote: ... Protein A/G plus agarose suspension solution was obtained from Calbiochem (MERCK) and Dimethyl Sulfoxide (DMSO ...
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bioRxiv - Microbiology 2023Quote: ... Wells were then washed six times with PBST and added with human fibrinogen (500 ng/well, PLG depleted; Calbiochem) and uPA (1 U/well) ...
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bioRxiv - Genetics 2021Quote: ... cells were treated with 50 μM of the protein synthesis inhibitor cycloheximide (Calbiochem), or ethanol as vehicle control during 4 h or 8 h ...