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Citations for Calbiochem :
351 - 400 of 487 citations for Ciprofloxacin Hcl 100 Ug Ml In Methanol 2 3 Carboxyl 13C3 99%; Quinoline 15N 98% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... In brief, lysis buffer (8 M urea, 1% Triton-100, 10 mM dithiothreitol, and 1% Protease Inhibitor Cocktail (Calbiochem) was used to extract the total protein ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were then harvested using lysis buffer (50mM HEPES pH7.4, 150mM NaCl, 1mM MgCl2, 1mM NaF, 1% Triton-x-100, protease inhibitor cocktail I [Calbiochem) and centrifuged at 14,000rpm for 10 minutes ...
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bioRxiv - Cancer Biology 2022Quote: ... collected and lysed in lysis buffer RGMT (50mM HEPES pH 7.4, 150mM NaCl, 1mM MgCl2, 1mM NaF, 1% Triton-x-100, plus protease inhibitor cocktail I [Calbiochem]). Cellular lysates were cleared by centrifugation and 225µL of total protein extract was incubated with biotin-tagged CIGB-300 (100μM ...
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bioRxiv - Neuroscience 2021Quote: ... cells were lysed in mild non-ionic detergents (0.1% Triton X-100 and Deoxycholate) and protease inhibitor cocktail (Calbiochem) to preserve the integrity of histones harbouring epitopes of interest during cell lysis ...
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bioRxiv - Bioengineering 2022Quote: ... The microfluidic devices were then washed three times with PBS and permeabilized for 20 min with 0.1% Triton X-100 (Calbiochem) diluted in PBS ...
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bioRxiv - Cancer Biology 2023Quote: ... The specimens were probed with specific primary antibodies (anti-p53 (DO-1, 1:100, EMD Millipore, Calbiochem, Darmstadt, Germany), anti-ARF6 (ab77581 ...
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bioRxiv - Cancer Biology 2022Quote: ... Boston Bioproducts (Cat# BP-115X) supplemented with protease inhibitor cocktail at a dilution of 1: 100 (Calbiochem, Cat#539131) and phosphatase inhibitors at a dilution of 1 ...
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bioRxiv - Molecular Biology 2023Quote: ... 10 mM EDTA, 1% NP-40, 1% sodium deoxycholate, 0.1% SDS, 1 mM DTT, 5 mM PMSF, and 1:100 Calbiochem Complete Protease Inhibitor Cocktails VII and V ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... and 0.2% soybean phosphatidylcholine with 100 μl of protease inhibitor cocktail (Protease Inhibitor Cocktail Set III, EDTA-Free, Calbiochem). After stirring for 1 hour at 4°C ...
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bioRxiv - Biochemistry 2019Quote: ... an oxygen-scavenging system (0.2% glucose, glucose oxidase (0.11 mg/ml; Calbiochem), and catalase (0.018 mg/ml ...
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bioRxiv - Genetics 2020Quote: ... RNA was removed by adding 50 µg/ml of RNAse A (CalBiochem) in each tube which was incubated at room temperature for 1 h and then DNA was purified according the protocol described by [23] ...
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bioRxiv - Biochemistry 2021Quote: ... cells were semi-permeabilsed using 80 μg/mL high purity digitonin (Calbiochem) and treated with 0.2 U Nuclease S7 Micrococcal nuclease from Staphylococcus aureus (Sigma-Aldrich ...
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bioRxiv - Genetics 2022Quote: ... To maintain the selective pressure for immortalization 0.1mg/mL G418 Sulfate (Calbiochem) was added ...
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bioRxiv - Biochemistry 2022Quote: ... DNA was stained with 0.5 μg/ml DAPI (Serva) and Mowiol (Calbiochem) was used as mounting media.
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bioRxiv - Biochemistry 2022Quote: ... DNA was stained with 0.5 μg/ml DAPI (Serva) and Mowiol (Calbiochem) was used as mounting media.
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bioRxiv - Microbiology 2021Quote: ... 200 µg/ml pronase (from Steptomyces griseus, Calbiochem, Merck KGaA, Darmstadt, Germany) were added and the sample was incubated overnight at 37 °C ...
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bioRxiv - Cell Biology 2021Quote: ... and stable transfectants were selected with 400 μg/ml G418 Sulphate (Calbiochem). The stable transfectants were enriched for the expression of CD4 by FACS ...
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bioRxiv - Biochemistry 2021Quote: ... DNA was stained with 0.5 μg/ml DAPI (Serva) and Mowiol (Calbiochem) was used as mounting media ...
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bioRxiv - Bioengineering 2022Quote: ... (4) LPS for 90 min and 10 μg/ml Actinomycin D (Calbiochem) after 45 min ...
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bioRxiv - Immunology 2022Quote: ... we used PMA (10 ng/mL; Alexis) and ionomycin (1 μM; Calbiochem). In some experiments ...
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bioRxiv - Cell Biology 2023Quote: ... and 10 µg/ml colchicine (cat#D00138122, Calbiochem, San Diego, CA, USA) then incubated for 6 h at 37 °C at 5% CO2 ...
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bioRxiv - Cell Biology 2022Quote: ... For rapamycin treatment stock solution (400 μg/ml in DMSO; Calbiochem #553210) was diluted 1:1000 to a final concentration of 400 ng/ml ...
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bioRxiv - Cell Biology 2023Quote: ... DNA was stained with 0.5 μg/ml DAPI (Serva) and Mowiol (Calbiochem) was used as mounting media.
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bioRxiv - Neuroscience 2023Quote: ... cultures were treated with 1.4 μg/mL cytosine-β-D-arabinofuranoside (Calbiochem) for three days to suppress division of non-neuronal cells ...
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bioRxiv - Biophysics 2022Quote: ... [Ca2+]ER levels were assessed by the addition of 2 µM ionomycin (IO; Calbiochem, San Diego, CA), a Ca2+ ionophore ...
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bioRxiv - Molecular Biology 2020Quote: ... Lyophilized MEISi-1 and MEISi-2 small molecules were dissolved in sterile dimethyl sulfoxide (DMSO, Calbiochem, 317275). Luciferase reporter assays were carried out as we have done previously 13,14,16,17 ...
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bioRxiv - Neuroscience 2021Quote: ... they were incubated for 2 h in secondary antibody (anti-mouse fluorescein conjugated, Calbiochem cat n. 401234) 1:100 in BSA 1% + sheep serum 2% in PBT ...
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bioRxiv - Immunology 2020Quote: ... Rac1 inhibitor (NSC23766) and Arp-2 complex inhibitor I (CK-666) & II (CK-869) purchased from Calbiochem were used for blocking actin assembly (33).
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bioRxiv - Neuroscience 2023Quote: Cortical neurons were incubated with 4 μM fura-2 acetoxymethyl ester (AM, Calbiochem, CAS 108964-32-5) 40 min at 37°C ...
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bioRxiv - Immunology 2023Quote: ... NF-κB (BAY11-7082; 2 μM) and RAF1 (RAF1 Kinase inhibitor I; 10 μM) were from Calbiochem. The inhibitor of EGFR (gefitinib ...
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bioRxiv - Cell Biology 2021Quote: ... Vibratome sections were cut to a thickness of 100 μm using a Leica VT1000S vibratome and mounted in Mowiol (Calbiochem). Confocal images were captured on a Zeiss Axiovert 200 upright microscope stand with LSM710 meta confocal scanner (63X objective NA 1.40) ...
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bioRxiv - Molecular Biology 2019Quote: ... 1 mM EDTA, 150 mM NaCl, 1% Triton X-100, 1 mM PMSF and 1X protease inhibitor cocktail III [Calbiochem]). After a 5-minute incubation on ice ...
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bioRxiv - Systems Biology 2020Quote: ... cell lysis buffer (1X phosphate buffered saline (PBS) with 1% TrintonX-100 and 1% EDTA-free protease inhibitor (Calbiochem, #539134)) was added to each well and the plate was put on ice for 30 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... pH 7.6, 1 mM EDTA, 1% Triton X-100, 0.1% sodium deoxycholate, 1 mM DTT, and protease inhibitor cocktail [Calbiochem, #539134]). The beads were incubated in a microcentrifuge tube with 5 µg of anti-FLAG antibodies (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2023Quote: ... pH 7.6, 1 mM EDTA, 1% Triton X-100, 0.1% sodium deoxycholate, 1 mM DTT, and protease inhibitor cocktail [Calbiochem, #539134]). The beads were incubated in a microcentrifuge tube with 3 µg rabbit anti-SDC-3 (lab stock) ...
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bioRxiv - Cell Biology 2023Quote: ... pH 7.6, 1 mM EDTA, 1% Triton X-100, 0.1% sodium deoxycholate, 1 mM DTT, and protease inhibitor cocktail [Calbiochem, #539134]) for every 1 gram of animals ...
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bioRxiv - Cell Biology 2023Quote: ... pH 7.6, 1 mM EDTA, 1% Triton X-100, 0.1% sodium deoxycholate, 1 mM DTT, and protease inhibitor cocktail [Calbiochem, #539134]) for every 1 gram of embryos and homogenized with 50 strokes in a Dounce homogenizer ...
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bioRxiv - Cell Biology 2022Quote: ... and an oxygen scavenging system composed of 216 μg/mL glucose oxidase (Calbiochem), 36 μg/mL catalase (Sigma) ...
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bioRxiv - Molecular Biology 2020Quote: ... according to manufacturer’s protocol and selected in 200 ng/mL Hygromycin B (Calbiochem) for one month ...
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bioRxiv - Genetics 2021Quote: ... the transfected cell library was selected with 5 μg/mL puromycin (540411, Calbiochem) for 12 additional days ...
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bioRxiv - Cell Biology 2021Quote: ... BMP4 50ng/mL and SB431542 5μM(Sigma-Aldrich, CAS 301836-41-9-Calbiochem) to generate endothelial cells ...
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bioRxiv - Microbiology 2022Quote: ... Mtb-lux were treated with 50 μg/ml gentamicin (Calbiochem, San Diego, CA) for 24 h to generate killed Mtb ...
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bioRxiv - Physiology 2021Quote: ... and phosphatase inhibitors (2 μM microcystin-LR, and 1× Phosphatase-Inhibitor Sets I and II (Calbiochem, Hayward, CA). We centrifuged homogenates at 4,000 g for 15 minutes to remove debris ...
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bioRxiv - Pathology 2023Quote: ... 2% DDM) supplemented with 1:1000 (v/v) Protease Inhibitor Cocktail Set III (Calbiochem, La Jolla, CA, USA). Rat anti-HA (3F10 ...
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bioRxiv - Cell Biology 2023Quote: ... mouse organs were homogenized in 2% SDS lysis buffer containing protease inhibitor mixture III (# 535140, Calbiochem, Billerica, MA) and phosphatase inhibitors (#P2850 ...
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bioRxiv - Microbiology 2019Quote: ... and scraped into 100 uL ice-cold RIPA lysis buffer containing 1X protease inhibitor cocktail III (Calbiochem, EMD Millipore, Darmstadt, Germany) and 1X PhosSTOP phosphatase inhibitor cocktail (Roche ...
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bioRxiv - Molecular Biology 2023Quote: ... 0.1 mM EGTA, 1% Triton X-100, 0.1% sodium deoxycholate, 0.5% SLS, 0.1% SDS, 0.5 mM PMSF, Calbiochem Complete Protease Inhibitor Cocktail V). The suspension was incubated for 30 min at 4°C and then sonicated with Covaris ME220 focused-ultrasonicator (50 alternating 15-s ON and 45-s OFF intervals ...
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bioRxiv - Biochemistry 2020Quote: ... and stable pools were selected with medium containing 500 µg ml-1 G418 (Calbiochem). The expression of the indicated genes in the stable pools was induced by the addition of 1 µg ml-1 doxycycline (Sigma ...
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bioRxiv - Microbiology 2022Quote: Transfected S2 cells transferred to coverslips precoated with 0.5 mg/mL Concanavalin A (Calbiochem) in 12 well plates for 2 hours ...
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bioRxiv - Systems Biology 2019Quote: ... Cells were stimulated with either 10ng/mL LPS (Salmonella enterica serovar Minnesota R595; Calbiochem), TNF (R&D Systems ...