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Citations for Calbiochem :
51 - 98 of 98 citations for C Reactive Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2023Quote: ... and the C-class iPSCs with a mixture of two inhibitors (2i): 1 μM MEK inhibitor (PD0325901, Calbiochem) and 3 μM GSK3 inhibitor (CH99021 ...
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bioRxiv - Cell Biology 2020Quote: ... Wnt-surrogate55 (0.15 nM, U-Protein Expression) and Rho kinase inhibitor (10 μM, Calbiochem Y27632) was added to the media whenever cells were at a single-cell stage to help recovery ...
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bioRxiv - Cancer Biology 2022Quote: ... Immunodetection of RAS proteins was carried out with pan-RAS (Ab-3; Calbiochem; 1:1,000) antibodies ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were rinsed with cold PBS1X and then lysed in Triton-X100 buffer at 4°C (50mM HEPES pH 7.4, 150mM NaCl, 1 % Triton X-100, protease inhibitors (Calbiochem) and phosphatase inhibitor cocktails II and IV (Calbiochem)) ...
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bioRxiv - Plant Biology 2020Quote: ... Homogenate was centrifuged at 10,000 x g at 4°C for 10 minutes and filtered through two layers of Miracloth (Calbiochem) before centrifugation at 40,000 x g for one hour at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... 50 μg of the total RNA was incubated for 1 h at 4 °C with 10 μl of the anti-2,2,7-trimethylguanosine-antibody (Calbiochem Milipore) coupled to sepharose beads (Fig ...
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bioRxiv - Cancer Biology 2022Quote: ... The viability of the cells was then evaluated by incubating at 37 °C for 2.5 h with 200 mg/ml MTT (Calbiochem). The MTT solution was removed afterwards ...
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bioRxiv - Developmental Biology 2020Quote: ... allowed to dry for 30 min at 37 °C and mounted in MOWIOL 4-88 Reagent (Calbiochem, Darmstadt, Germany).
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bioRxiv - Immunology 2022Quote: ... and 1 × 106 cells were treated with rapamycin (10 ng/ml, 30 min, 37°C; Calbiochem, San Diego, CA) prior to treatment with rhGH.
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bioRxiv - Microbiology 2023Quote: ... Plates were then incubated at 37 °C for 2 h with different quantities (0.125 - 4 pmol) of biotinylated PfPP1c labeled with biotin-NHS (Calbiochem) in PBS-Tween 0.1% ...
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bioRxiv - Plant Biology 2023Quote: ... The homogenate was centrifuged at 4°C for 15 min at 10,000×g and the supernatant was filtered through Miracloth (Calbiochem) to remove plant debris ...
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bioRxiv - Immunology 2021Quote: ... GMDCs were incubated for 2 h at 37°C with 50 µM MARK inhibitor (MARK/Par-1 Activity Inhibitor, 39621; Calbiochem), 50 nM SIK inhibitor (HG-9-91-01 ...
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bioRxiv - Neuroscience 2021Quote: ... allowed to dry for 30 min at 37 °C and mounted in MOWIOL® 4-88 (Calbiochem, Darmstadt, Ger-many).
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bioRxiv - Cell Biology 2019Quote: ... and incubated overnight at 4 °C with mouse monoclonal antibodies against type I collagen (20 μg/mL, CP-17, Calbiochem), type II collagen (0.4 μg/mL ...
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bioRxiv - Cell Biology 2019Quote: ... and Scott 2007) stably expressing ARL13B C-terminally tagged with GFP were generated by lentiviral infection followed by puromycin (Calbiochem) selection.
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bioRxiv - Biochemistry 2021Quote: ... Cells were washed twice with 3 ml PBS at 400 g for 5 minutes at 4°C and 500 uL PI staining buffer was added (1X PBS, 50 μg/mL PI (Calbiochem), 2μg/mL RNase A (Sigma ...
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bioRxiv - Cell Biology 2020Quote: ... Samples were labeled with the indicated primary antibodies overnight at 4°C in the presence of 0.01% saponin (Calbiochem, 558255) and 1% BSA ...
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bioRxiv - Molecular Biology 2021Quote: BL21-RP cells co-transformed with pGEX4T1/GST-NXF1 and pET9a/p15 were induced overnight at 18°C with 0.4 mM isopropyl-β-D-thiogalactoside (IPTG, Calbiochem) in Terrific Broth ...
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bioRxiv - Immunology 2022Quote: ... Cells were left unstimulated or stimulated for indicated time at 37 °C with PMA (10 ng/ml; Alexis) and ionomycin (1 μM; Calbiochem) or solvent (DMSO) ...
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bioRxiv - Cell Biology 2023Quote: ... the cells were fixed with cold methanol (10 min at −20°C) and blocked for 30 min with 3% (w/v) bovine serum albumin (Calbiochem) in 1 x PBS at room temperature ...
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bioRxiv - Pathology 2024Quote: ... NETs formation was induced for 3 hours at 37°C and 5% CO2 by stimulation with 10 µM of PAF (Calbiochem) on primed neutrophils and 25 µM on non-primed ones ...
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bioRxiv - Bioengineering 2020Quote: ... The light/heavy peptide mixture was incubated with 60 μL protein G agarose bead slurry (Calbiochem) conjugated to an antibody cocktail containing 12 μg 4G10 (Millipore) ...
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bioRxiv - Biochemistry 2020Quote: ... all fractions were individually precipitated using the Proteoextract Protein Precipitation Kit (EMD Milipore, Calbiochem, 539180-1KIT). For each 600 µL fraction ...
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bioRxiv - Microbiology 2023Quote: ... The reaction mixture contains 8 μg total proteins and 4 μg nitrocefin (Calbiochem, San Diego, CA). β-Lactamase activity was determined by measuring the absorbance at 486 nm (OD486nm).
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... free-floating sections were washed with KPBS to remove the cryoprotectant solution and then incubated directly with polyclonal rabbit anti-c-Fos (PC-38; Calbiochem-Millipore) at 1:20000 in 0.5% Blocking Reagent (Roche ...
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bioRxiv - Biophysics 2020Quote: L-asparaginase samples were diluted to a final concentration of 280 μM and incubated at 25°C with 0.9 μg/ml of Proteinase K (Calbiochem, Cat# 539480) in the absence or presence of a 10-fold excess of L-Asp or L-Glu (2.8 mM) ...
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bioRxiv - Neuroscience 2019Quote: ... we incubated live DIV21 hippocampal cultures infected with control or UPF2 shRNAs at 37°C for 5 min with GluR1 antibody (Calbiochem, PC246) to allow labeling of surface GluR1 ...
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bioRxiv - Neuroscience 2021Quote: One series of sections was washed with KPBS to remove the cryoprotectant solution and then incubated with polyclonal rabbit anti-c-Fos (PC-38; Calbiochem-Millipore) at 1:20000 in 0.5% Blocking Reagent (Roche ...
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bioRxiv - Neuroscience 2022Quote: ... the cells were then incubated overnight at 4°C with primary antibodies (rabbit anti-AUTS2, 1:200, Atlas Antibodies; rabbit anti-GRIA1, 1:10, Calbiochem; mouse anti-GRIA2 ...
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bioRxiv - Cancer Biology 2020Quote: Whole cell protein lysates were prepared in RIPA buffer supplemented with Phosphatase Inhibitor Cocktail Set-II (Calbiochem) and protease inhibitor (VWR ...
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bioRxiv - Neuroscience 2023Quote: ... after which the gels was washed with water and all protein was stained using RAPIDstain (Calbiochem, 553215) for 1 hour ...
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bioRxiv - Physiology 2019Quote: ... Individual gel slices were subjected to in-gel reduction with dithiothreitol (10 mM, 30 min, 56 °C, Merck-Calbiochem, San Diego, USA), alkylation with iodoacetamide (50 mM ...
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bioRxiv - Genetics 2022Quote: ... an aliquot of the cushion supernatant and eluate were precipitated using the ProteoExtract protein precipitation kit (Calbiochem 539180) per manufacturer protocol and redissolved in 2X Laemmli buffer (Fisher 50-196-784) ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: Brain tissue was homogenized in RIPA buffer supplemented with protease and protein phosphatase inhibitors (Calbiochem, La Jolla, CA) and subjected to SDS-PAGE followed by transferring to PVDF membranes (Millipore ...
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bioRxiv - Physiology 2023Quote: Total protein extracts were obtained from microalgal cells in exponential phase of growth (e.g. 4 days) in 50 mM Tris buffer pH 8.0 supplemented with protein inhibitor cocktail (539131, Calbiochem). Free-living and symbiotic microalgae were disrupted by bead beating with a Precellys device (Bertin Technologies ...
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bioRxiv - Neuroscience 2021Quote: ... cells were rinsed two times with pre-warmed medium and incubated for 30 min at 37°C with rabbit anti-GluA1 (1/150; Calbiochem/Millipore, RRID: AB_564636) in culture medium ...
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bioRxiv - Cancer Biology 2020Quote: Dissected CP specimens were dissociated using forceps under a stereoscope followed by enzymatic digestion at 37°C for 20 minutes with pronase (2 mg/ml, 537088, Calbiochem, San Diego, CA) in Hank’s balanced salt solution (HBSS ...
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bioRxiv - Neuroscience 2019Quote: ... Fyn was IPed with 1µg Fyn antibody (mouse monoclonal antibody [mAb], sc-434, SantaCruz) and 10 µL Protein G plus-agarose (CalBiochem). Washed Protein G bead pellets were resuspended in 2X Laemmli buffer and subjected to Western blot analysis ...
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bioRxiv - Molecular Biology 2020Quote: ... or with polyclonal anti-Sir3 antibodies and immune complexes were collected with Protein G/A beads (Calbiochem, EMD Biosciences). Immunoprecipitated and input DNA were purified using Chelex 100 (Bio-Rad ...
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bioRxiv - Microbiology 2019Quote: ... Lysates were cleared by centrifugation and precipitated with anti-HA antibody (clone HA-7; Bioshop) using agarose protein G beads (Calbiochem) as previously described25 ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins were extracted using an 8M urea lysis buffer (8M urea, 50 mM Tris-HCl, 75 mM NaCl, 1x Calbiochem protease inhibitor ...
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bioRxiv - Plant Biology 2019Quote: ... After 30 min incubation on a rotator at 4°C the protein samples were centrifuged at 18,000xg for 30 min to remove plant debris and the protein extract was filtered through Miracloth filter paper (Calbiochem Co.). Next ...
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bioRxiv - Biochemistry 2022Quote: ... lysed in M-PER mammalian protein extraction reagent (Thermo-Fisher) with 1/1000 of the EDTA-free protease inhibitor cocktail Set III (Calbiochem), and centrifuged for 30 min at 15,000 × g at 4°C ...
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bioRxiv - Cell Biology 2021Quote: Commercially available antibodies against the following proteins were used for Western blotting in the indicated dilutions: BRCA2 (OP95, Calbiochem, 1:1000), RAD51 (70-001 ...
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bioRxiv - Cancer Biology 2020Quote: ... plate wells were washed with PBS containing 2% FBS and incubated overnight with biotinylated HA-binding protein (3 μg/ml, Calbiochem-EMD Millipore) at 4° C (34) ...
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bioRxiv - Cancer Biology 2020Quote: ... plate wells were washed with PBS containing 2% FBS and incubated overnight with biotinylated HA-binding protein (3 μg/ml, Calbiochem-EMD Millipore) at 4° C (16) ...
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bioRxiv - Cell Biology 2021Quote: ... plate wells were washed with PBS containing 2% FBS and incubated overnight with biotinylated HA-binding protein (3 μg/ml, Calbiochem-EMD Millipore) at 4° C (7) ...
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bioRxiv - Physiology 2023Quote: ... Normal goat serum was used as a blocking agent and slides were incubated in biotinylated hyaluronan binding protein (HABP, 1:500, CalBiochem, Burlington, MA) for one hour ...