Labshake search
Citations for Calbiochem :
51 - 100 of 104 citations for Alkaline Phosphatase Colorimetric Activity Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2023Quote: ... and phosphatase inhibitor cocktails (Calbiochem/Merk Millipore, San Diego, CA) on ice for 10 min ...
-
bioRxiv - Cell Biology 2021Quote: ... MARK/Par-1 activity inhibitor49 (MARK2i; Calbiochem 39621; 5 µM or 10 µM) was added prior to imaging ...
-
bioRxiv - Cancer Biology 2022Quote: ... Protease inhibitor cocktails and Phosphatase inhibitor cocktails were purchased from Calbiochem and SIGMA-ALDRICH ...
-
bioRxiv - Cancer Biology 2023Quote: ... radioimmunoprecipitation assay (RIPA) buffer with Phosphatase Inhibitor Cocktail Set-II (Calbiochem) and protease inhibitor (VWR ...
-
bioRxiv - Neuroscience 2023Quote: ... All buffers used were supplied with protease and phosphatase inhibitors (Calbiochem). Levels of Aβ peptides (amyloid-β1–38 ...
-
bioRxiv - Molecular Biology 2023Quote: ... The ATP analogue 1NM-PP1 was used to inhibit PKA activity (Calbiochem, Merck Millipore). Cells were grown to mid-log phase OD600 1 ...
-
bioRxiv - Immunology 2020Quote: Neutrophils were rapidly lysed in boiling Laemmli buffer containing phosphatase inhibitors (Calbiochem). Proteins were separated by SDS-PAGE on a 12% gel and transferred onto PVDF membrane (Millipore) ...
-
bioRxiv - Cancer Biology 2022Quote: ... and phosphatase inhibitors at a dilution of 1: 500 (Calbiochem, Cat# 524625) for 10 minutes at 4 °C and then centrifuged at 14.000g for 15 minutes (Centrifuge 5415 R) ...
-
bioRxiv - Molecular Biology 2020Quote: ... Proteasome activity was quantified using the AMC-tagged proteasome fluorogenic substrate (Succ-LLVY-AMC, Calbiochem), similarly to (33) ...
-
bioRxiv - Immunology 2023Quote: ... 4.3 mM Bromphenol Blue and 5% 2-mercaptoethanol) supplemented with phosphatase (Calbiochem, 524625) and protease (Roche ...
-
bioRxiv - Biochemistry 2023Quote: ... Protease inhibitor cocktail (539131) and phosphatase inhibitor cocktail (524625) were both from Calbiochem. Annexin V (A13201 ...
-
bioRxiv - Biochemistry 2023Quote: The peptidase activity of the 20S proteasome was measured with commonly used fluorogenic peptide substrates (Bachem, Calbiochem): succinyl-LeuLeuValTyr-4-methylcoumarin-7-amide (SucLLVY-MCA ...
-
bioRxiv - Cell Biology 2022Quote: ... Mouse pancreatic islets were lysed with radioimmunoprecipitation assay buffer containing protease and phosphatase inhibitors (Calbiochem), and insoluble material was removed by centrifugation ...
-
bioRxiv - Cancer Biology 2021Quote: ... and then lysed with radio-immunoprecipitation assay (RIPA) buffer containing protease and phosphatase inhibitor cocktail (Calbiochem). The protein concentrations of cell lysate supernatants were assayed using a BCA protein assay kit (Thermo Scientific) ...
-
bioRxiv - Cancer Biology 2020Quote: Whole cell protein lysates were prepared in RIPA buffer supplemented with Phosphatase Inhibitor Cocktail Set-II (Calbiochem) and protease inhibitor (VWR ...
-
bioRxiv - Immunology 2021Quote: ... GMDCs were incubated for 2 h at 37°C with 50 µM MARK inhibitor (MARK/Par-1 Activity Inhibitor, 39621; Calbiochem), 50 nM SIK inhibitor (HG-9-91-01 ...
-
bioRxiv - Neuroscience 2023Quote: ... O-GlcNAc transferase and O-GlcNAcase activities were blocked by 5 µM OSMI-4 (MedChem Express) and 5 µM Thiamet-G (Calbiochem) treatments ...
-
bioRxiv - Neuroscience 2020Quote: ... Total mouse hippocampus from the left hemisphere was homogenised using a mechanical homogeniser and disposable pestles (Anachem) in 100μl TBS (50mM Tris-HCl pH 8.0) containing protease and phosphatase inhibitors (Calbiochem). The homogenate was then centrifuged at 4 °C for 30 mins at 16,000 × g ...
-
bioRxiv - Cancer Biology 2022Quote: The cells were lysed in radioimmunoprecipitation assay buffer (RIPA buffer) supplemented with phosphatase inhibitor cocktail set-II (Calbiochem) and protease inhibitor (VWR) ...
-
bioRxiv - Animal Behavior and Cognition 2022Quote: Brain tissue was homogenized in RIPA buffer supplemented with protease and protein phosphatase inhibitors (Calbiochem, La Jolla, CA) and subjected to SDS-PAGE followed by transferring to PVDF membranes (Millipore ...
-
bioRxiv - Cell Biology 2019Quote: ... cells were lysed at 1 × 109 cells/ml in ice-cold lysis buffer (0.1 µM TLCK, 1 µg/ml Leupeptin, Phosphatase Inhibitor Cocktail II (Calbiochem),1 mM PMSF ...
-
bioRxiv - Neuroscience 2020Quote: ... A half cortex was weighed on a microscale and homogenised in 4 volumes of ice-cold Tris-buffered saline (TBS) (50mM Tris-HCl pH 8.0) containing a cocktail of protease and phosphatase inhibitors (Calbiochem) using a handheld mechanical homogeniser and disposable pestles (Anachem) ...
-
bioRxiv - Neuroscience 2020Quote: ... in approximately 5 volumes of ice-cold Tris-buffered saline (TBS) (50 mM Tris-HCL, pH 8.0) containing a cocktail of protease and phosphatase inhibitors (Calbiochem). Homogenates were centrifuged at 16,000 x g at 4 °C for 30 min ...
-
bioRxiv - Neuroscience 2020Quote: Weighed cortex was homogenised in Tris-buffered saline (TBS) (50 mM Tris-HCl pH 8.0) plus complete protease and phosphatase inhibitors (Calbiochem) before centrifugation at 175,000 × g for 30 minutes at 4°C ...
-
bioRxiv - Neuroscience 2022Quote: ... A half cortex was weighed on a microscale and homogenised in 4 volumes of ice-cold Tris-buffered saline (TBS) (50mM Tris-HCl pH 8.0) containing a cocktail of protease and phosphatase inhibitors (Calbiochem) using a handheld mechanical homogeniser and disposable pestles (Anachem) ...
-
bioRxiv - Neuroscience 2022Quote: A half cortex was weighed on a microscale and homogenised in 3 volumes of ice-cold Tris-buffered saline (TBS) (50mM Tris-HCl pH 8.0) containing a cocktail of protease and phosphatase inhibitors (Calbiochem) using a handheld mechanical homogeniser and disposable pestles (Anachem ...
-
bioRxiv - Molecular Biology 2022Quote: ... The cell pellet was lysed at 0.5 × 109 cells/ml in ice-cold lysis buffer (0.1 mM TLCK, 1 μg/ml Leupeptin, 1x Phosphatase Inhibitor Cocktail II tablet (Calbiochem), 1 mM PMSF ...
-
bioRxiv - Developmental Biology 2022Quote: ... Tissues were homogenized in cold lysis buffer (0.1% NP-40, 50mM Tris-HCl [pH 7.2], 250mM NaCl, 2mM EDTA, phosphatase inhibitor mixture I and II [Calbiochem] and one tablet of Minicomplete [Roche] per 10ml ...
-
bioRxiv - Neuroscience 2021Quote: ... we lysed the cortical neurons on ice with MLB buffer supplemented with anti-protease and anti-phosphatase cocktails (Calbiochem) and spun the lysates at 14,000 x g for 5 mins ...
-
bioRxiv - Cancer Biology 2023Quote: ... Caspase-3 activity was assessed in duplicates via a fluorogenic assay using the Ac-DEVD-AMC-specific caspase-3 substrate (Calbiochem, La Jolla, CA). After substrate addition ...
-
bioRxiv - Physiology 2021Quote: ... or 0.2 mL (for lung) HEPES-buffered saline plus Phosphatase Inhibitor Cocktail Set II and Protease Inhibitor Cocktail Set III (Calbiochem). Protein was measured using a Pierce™ BCA protein assay kit (Thermo Fisher ...
-
bioRxiv - Immunology 2020Quote: ... Cell lysates were collected in RIPA buffer plus inhibitors (protease: complete mini Roche; 11836153001, and phosphatases: cocktail set II Calbiochem; 524625) and then incubated with the antibody-bead mix overnight at 4°C on a shaker ...
-
bioRxiv - Cell Biology 2020Quote: ... and all cell types were lysed with Pierce™ RIPA Buffer (ThermoScientific 89900) supplemented with 1X Phosphatase Inhibitor Cocktail Set 2 (Calbiochem 5246251) and 1X Protease Inhibitor Cocktail Set 1 (Calbiochem 539131) ...
-
bioRxiv - Cancer Biology 2021Quote: ... cells were collected using 75μl Lysis Buffer (Cell Signaling Technology #9803, Phosphatase Inhibitor Cocktail Set II #524625 and Protease Inhibitor Cocktail Set III #539134 both Calbiochem). Upon cell lysis using a sonicator ...
-
bioRxiv - Cell Biology 2022Quote: ... a proteoExtract protein precipitation kit (Calbiochem, #539180) was used followed by mass spectrometry analysis (Taplin Biological Mass Spectrometry Facility ...
-
bioRxiv - Molecular Biology 2019Quote: The ProteoExtract® Subcellular Proteome Extraction Kit (Calbiochem) was used to generate lysates from the cytosolic ...
-
bioRxiv - Immunology 2021Quote: ... then precipitated with the ProteoExtract Protein Precipitation Kit (Calbiochem). Equal amounts of protein for each set of samples (e.g ...
-
bioRxiv - Immunology 2022Quote: ... prior to protein precipitation (Calbiochem Proteo-Extract precipitation kit). Precipitants were resuspended in 1% SDS and quantified by BCA assay for normalization ...
-
bioRxiv - Biochemistry 2024Quote: ... Proteins were precipitated out using ProteoExtract precipitation kit (Calbiochem) and dissolved in 1X Laemmli buffer ...
-
bioRxiv - Immunology 2021Quote: ... Proteins were precipitated with the ProteoExtract Protein Precipitation Kit (Calbiochem) and quantified by Coomassie (Bradford ...
-
bioRxiv - Evolutionary Biology 2019Quote: ... we used the Superoxide Dismutase Assay Kit II (catalogue no. 574601, Calbiochem, supplied by Merck Pty Ltd. ...
-
bioRxiv - Systems Biology 2021Quote: ... The proteins were then precipitated using Proteoextract protein precipitation kit (Calbiochem; cat # 539180) according to manufacturer’s instructions ...
-
bioRxiv - Microbiology 2022Quote: ... the cytoskeleton) were extracted using the “ProteoExtract Subcellular Proteome Extraction kit” (Calbiochem/ MERCK) according to the supplier’s instructions ...
-
bioRxiv - Developmental Biology 2021Quote: ... All primary/secondary antibodies were diluted in SignalBoost™ Immunoreaction Enhancer Kit (407207–1KIT, Calbiochem). After three washes in PBS ...
-
bioRxiv - Neuroscience 2020Quote: TUNEL staining was performed using the FragEL DNA Fragmentation Detection Kit from Calbiochem (EMD Millipore). 10-day old adult fly brains were dissected in PBST and then fixed in 4% paraformaldehyde for 20 minutes on ice ...
-
bioRxiv - Biochemistry 2020Quote: ... all fractions were individually precipitated using the Proteoextract Protein Precipitation Kit (EMD Milipore, Calbiochem, 539180-1KIT). For each 600 µL fraction ...
-
bioRxiv - Biophysics 2020Quote: ... The cells were washed three times in the washing buffer 1X of OxyDNA Assay Kit (500095, Calbiochem) and incubated overnight at 37°C in FITC-conjugated anti 8-oxoguanine (500095 ...
-
bioRxiv - Developmental Biology 2021Quote: Native membrane proteins were extracted from transfected cells using the ProteoExtract® Native Membrane Protein Extraction Kit (Calbiochem). Proteins from whole cells and from the membrane and soluble fractions were separated on a 12% SDS-PAGE protein gel ...
-
bioRxiv - Neuroscience 2021Quote: Cytoplasmic and nuclear fractions were prepared from cell lysates using a ProteoExtract® Subcellular Proteome Extraction Kit (Calbiochem) according to the manufacturer’s instructions ...
-
bioRxiv - Genetics 2022Quote: ... an aliquot of the cushion supernatant and eluate were precipitated using the ProteoExtract protein precipitation kit (Calbiochem 539180) per manufacturer protocol and redissolved in 2X Laemmli buffer (Fisher 50-196-784) ...