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Citations for Calbiochem :
451 - 500 of 650 citations for 8 phenylamino 5 4 5 sulpho 1 naphthyl azo 1 naphthyl azo naphthalene 1 sulphonic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2019Quote: ... Membranes were incubated in 1:1000 diluted primary antibodies pERK1/2 pT202/Y204 (Calbiochem cat# KP26001), total ERK1/2 (Cell Signaling cat# 9102) ...
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bioRxiv - Cell Biology 2021Quote: ... two other kinase inhibitors were used: 1 μM and 1.5 μM of AKT VIII (Calbiochem #124018) or 100 nM and 200 nM of trametinib (Selleckchem #GSK1120212) ...
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bioRxiv - Neuroscience 2022Quote: ... Nuclei were counterstained with 1 µg/ml of DAPI and sections were mounted with Fluorsave (Calbiochem).
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bioRxiv - Neuroscience 2021Quote: ... #MCA1957) and peptide-rich sensory nerve fibres with a rabbit anti-CGRP (1:2,000, Calbiochem, #PC205L). After washes ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... PamChip arrays (PamGene #88101) were blocked for 20 cycles with 1% Bovine serum albumin (Calbiochem #126609) in Tris-buffered saline (TBS) ...
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bioRxiv - Neuroscience 2023Quote: ... protease inhibitors (PI, 1:4000 dilution of protease inhibitor cocktail 3 w/o EDTA, Calbiochem, #539134) and benzonase (1:1000 ...
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bioRxiv - Developmental Biology 2023Quote: Cells were harvested in RIPA buffer with 1:100 protease inhibitor cocktail set I (Calbiochem, #539131) and phosphatase inhibitors (1:100 Na2Ov4 ...
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bioRxiv - Microbiology 2019Quote: ... or pronase (a mixture of endo- and exoproteases from Streptomyces griseus at a final concentration of 5, 10 or 50 μg/ml; Calbiochem, UK) for 30 min ...
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bioRxiv - Microbiology 2019Quote: ... media in the apical chamber were replaced with complete DMEM/F12 plus 5 μM of the γ-secretase inhibitor IX (DAPT; Calbiochem) while the basolateral media were replenished with the 1:1 stem cell/L-WRN media with 10μM Y-27632 (Calbiochem ...
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bioRxiv - Neuroscience 2019Quote: ... we incubated live DIV21 hippocampal cultures infected with control or UPF2 shRNAs at 37°C for 5 min with GluR1 antibody (Calbiochem, PC246) to allow labeling of surface GluR1 ...
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bioRxiv - Microbiology 2022Quote: ... For fungal culture filtrates a 106 spores/ml culture of RPMI was incubated at 37 ºC and 5% CO2 for 48 hours and then filtered through five layers of Miracloth (Calbiochem, UK) and then a 0.45 μm syringe filter to remove any spores and hyphal fragments ...
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bioRxiv - Immunology 2023Quote: ... 4-iodo-6-phenylpyrimidine (4-IPP) (Calbiochem CAS 41270) was injected intraperitoneally at 5 mg/kg every 48 hours.
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bioRxiv - Cell Biology 2019Quote: ... cells were resuspended in KHM buffer containing CaCl2 (1 mM) and treated with Stapylocossus aureus nuclease (Calbiochem) (150 U/ml ...
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bioRxiv - Molecular Biology 2020Quote: ... Pellets were resuspended in 300 µl of DMEM 2% FBS 1 µg/mL propidium iodide (Calbiochem, 537060) and filtered through a 40-µm cell strainer (BD Falcon ...
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bioRxiv - Molecular Biology 2019Quote: ... ATP regeneration system (1 mM ATP, 25 mM creatine monophosphate [Sigma], 0.03 U/µL creatine kinase [Calbiochem] ...
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bioRxiv - Molecular Biology 2020Quote: ... Lyophilized MEISi-1 and MEISi-2 small molecules were dissolved in sterile dimethyl sulfoxide (DMSO, Calbiochem, 317275). Luciferase reporter assays were carried out as we have done previously 13,14,16,17 ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The master mix recipe contained polyclonal anti-rabbit anti-MLH1 (Calbiochem, San Diego CA; diluted 1:50) or anti-rabbit anti-DMC1 (mix of DMC1) ...
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bioRxiv - Cell Biology 2022Quote: ... resuspended in 6 ml lysis buffer (1X PBS, 500 mM NaCl, 1% Triton X-100, lysozyme, Calbiochem EDTA-free protease inhibitor cocktail set III ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were washed with ice cold PBS and lysed in IP lysis buffer (50 mM Tris, pH 7.5, 10 mM NaCl, 2.5 mM MgCl2, 1 mM DTT, 0.01% digitonin [Calbiochem 300410] and protease inhibitors ...
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bioRxiv - Cell Biology 2023Quote: ... Controls were treated for 30 minutes with KRBH + 2.8 mM glucose + 1 μM latrunculin B (Calbiochem, #428020) or 1 μM jasplakinolide (Millipore Sigma ...
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bioRxiv - Cell Biology 2023Quote: ... GFP-Rab11 WTlow and DNlow cell culture media was also supplemented with 1.25 μg.mL-1 puromycin (Calbiochem). Cells were regularly tested for mycoplasma contamination with the LookOut mycoplasma PCR detection kit (Sigma ...
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bioRxiv - Microbiology 2023Quote: ... cells were pretreated 1 h at 37°C with 20 nM bafilomycin A1 (Calbiochem, stock in DMSO) to block endocytosis ...
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bioRxiv - Neuroscience 2023Quote: ... The following primary antibodies were used: polyclonal rabbit anti-tyrosine hydroxylase (TH, catalog # 657012, 1:1000, Calbiochem), monoclonal mouse anti-TH (catalog # MAB318 ...
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bioRxiv - Neuroscience 2023Quote: ... incubated overnight in TBST with 1% bovine serum albumin (BSA; Calbiochem by Millipore Sigma, St. Louis, MO) and primary antiserum (Millipore Sigma MAB318 ...
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bioRxiv - Neuroscience 2020Quote: Mice were submitted to 24 h of fasting and then received ip injections with either saline (0.9% NaCl) or mouse recombinant leptin (5 mg/kg body weight, Calbiochem, Billerica, MA, USA) immediately before the dark cycle ...
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bioRxiv - Immunology 2021Quote: ... The cells were then treated with SplB (5 μg/mL) or increasing concentrations of human plasma thrombin (PAR1 and PAR4 agonist, Calbiochem-EMD Millipore) or porcine trypsin (PAR2 agonist ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were treated with 5,8,11,14-Eicosatetraynoic acid (ETYA, Calbiochem).
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bioRxiv - Cell Biology 2022Quote: ... 50 nM okadaic acid (PP1 and PP2A inhibitor, Calbiochem), 2 μM tautomycetin (PP1 inhibitor ...
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bioRxiv - Physiology 2023Quote: ... Fatty acid free BSA was obtained from CalBioChem (#126575). Polyethylene glycol 300 (PEG300 ...
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bioRxiv - Neuroscience 2019Quote: ... diluted in KPBS containing 0.3 % Triton X-100 and incubated overnight at RT in cFos rabbit polyclonal antiserum (1:20,000; Calbiochem) diluted in KPBS containing 1 % NGS and 0.3 % Triton X-100 ...
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bioRxiv - Cell Biology 2020Quote: ... and the cells were washed with ice-cold PBS(-/-) with 1X Protease Inhibitor Cocktail Set 1 (Calbiochem 539131). The PBS was aspirated ...
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bioRxiv - Neuroscience 2020Quote: ... 14 – 17 DIV hippocampi cultures were stained live with a GluA1 antibody (GluA1 Calbiochem PC246, rabbit, 1:20) for 15 min at 37°C in a humidified chamber ...
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bioRxiv - Physiology 2021Quote: ... and phosphatase inhibitors (2 μM microcystin-LR, and 1× Phosphatase-Inhibitor Sets I and II (Calbiochem, Hayward, CA). We centrifuged homogenates at 4,000 g for 15 minutes to remove debris ...
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bioRxiv - Biochemistry 2021Quote: ... Cell pellets were sonicated in 20 mM HEPES buffer pH 7.5 containing 0.25 M sucrose and 1:100 protease inhibitor cocktail set V (Calbiochem) and centrifuged at 2,000 × g for 10 min to pellet nuclear fraction ...
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bioRxiv - Biochemistry 2021Quote: ... Data were normalized to maximal inhibition determined by 1 μM decanoyl-Arg-Val-Lys-Arg-chloromethylketone (Calbiochem #344930).
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bioRxiv - Neuroscience 2021Quote: ... or P5–P6 DRG rat neurons were diluted to 35,000 cells/ml in Sato’s media and treated with either 1 mM dbcAMP (Calbiochem), mixed population or recombinant Histones (1-20µg/ml ...
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bioRxiv - Immunology 2020Quote: ... Cells were grown at 37°C to OD = 0.8 and expression was induced with 0.3 mM IPTG (Isopropyl b-D-1-thiogalactopyranoside, Calbiochem) overnight at 18°C ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... sections were incubated with rabbit anti-c-Fos antibody diluted with the blocking solution (1:20000, #PC38, Calbiochem) for 2 days at 4°C ...
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bioRxiv - Molecular Biology 2022Quote: ... Samples were incubated for 1 h with the ADAM17 inhibitor Batimastat (BB-94) at 10 μM (196440, Calbiochem), with the selective ADAM10 inhibitor GI254023X (GI ...
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bioRxiv - Pathology 2023Quote: ... 2% DDM) supplemented with 1:1000 (v/v) Protease Inhibitor Cocktail Set III (Calbiochem, La Jolla, CA, USA). Rat anti-HA (3F10 ...
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bioRxiv - Biophysics 2023Quote: ... and the C-class iPSCs with a mixture of two inhibitors (2i): 1 μM MEK inhibitor (PD0325901, Calbiochem) and 3 μM GSK3 inhibitor (CH99021 ...
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bioRxiv - Molecular Biology 2024Quote: ... The pellet was suspended in 2 ml of lysis/binding buffer (25 mM HEPES/KOH, pH 7.6, 10 % glycerol, 150 mM KCl, 1 mM dithiothreitol, 0.1 % Elugent (Calbiochem), loaded into SHREDDER tubes (Pressure Biosciences Inc ...
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bioRxiv - Neuroscience 2020Quote: Mice were submitted to 12 h of fasting and then received ip injections with mouse leptin (5 mg/kg body weight, Calbiochem, Billerica, MA, USA). After 1 h ...
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bioRxiv - Developmental Biology 2022Quote: ... mature female mice (6-9 weeks C57BL/6) were injected IP with 5 IU of pregnant mare serum gonadotropin (PMSG) (Calbiochem, San Diego, CA), followed 48 hours later by 5 IU of human chorionic gonadotropin (hCG ...
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bioRxiv - Immunology 2019Quote: ... or the inactive analog CK-689 (Calbiochem, #CAS 170930-46-8) (Hetrick et al. ...
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bioRxiv - Cancer Biology 2021Quote: ... and 2 mM glutamine for 24 h prior to treatment with either TGF-β (1 ng/mL, Millipore Calbiochem) or 10 uM TGF-β inhibitors (SB431542 ...
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bioRxiv - Cell Biology 2019Quote: ... cells were lysed at 1 × 109 cells/ml in ice-cold lysis buffer (0.1 µM TLCK, 1 µg/ml Leupeptin, Phosphatase Inhibitor Cocktail II (Calbiochem),1 mM PMSF ...
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bioRxiv - Biochemistry 2019Quote: ... which were grown to an optical density of 0.6–0.8 at 600 nm and were induced with 1 mM isopropyl-β-D-thiogalactopyranoside (CALBIOCHEM) for 20 h at 18 °C ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were then harvested using lysis buffer (50mM HEPES pH7.4, 150mM NaCl, 1mM MgCl2, 1mM NaF, 1% Triton-x-100, protease inhibitor cocktail I [Calbiochem) and centrifuged at 14,000rpm for 10 minutes ...
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bioRxiv - Cancer Biology 2022Quote: ... collected and lysed in lysis buffer RGMT (50mM HEPES pH 7.4, 150mM NaCl, 1mM MgCl2, 1mM NaF, 1% Triton-x-100, plus protease inhibitor cocktail I [Calbiochem]). Cellular lysates were cleared by centrifugation and 225µL of total protein extract was incubated with biotin-tagged CIGB-300 (100μM ...