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Citations for Calbiochem :
301 - 350 of 350 citations for 7 CHLORO 10 11 DIHYDRO 5H DIBENZ B F ACEPIN 2 OL since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... and 1 × 106 cells were treated with rapamycin (10 ng/ml, 30 min, 37°C; Calbiochem, San Diego, CA) prior to treatment with rhGH.
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bioRxiv - Biochemistry 2023Quote: ... 0.1% DDM, 10% (v/v) ethylene glycol, 0.005% asolectin (Avanti Polar Lipid, USA) and 0.005% CHAPS (Calbiochem, Merck, Germany), and eluted by an increasing proportion of the same buffer plus 1 M NaCl ...
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bioRxiv - Cell Biology 2019Quote: ... (N-{(2S,4R)-4-(Biphenyl-2-ylmethyl-isobutyl-amino)-1-[2-(2,4-difluorobenzoyl)-benzoyl]-pyrrolidin-2-ylmethyl}8-3-(4-(2,4-dioxothiazoldin-5-ylidenemethyl)-phenyl]acrylamide (cPLA2 inhibitor, cPLA2 Inh, Calbiochem, EMD Biosciences), was used concomitantly with priming at 5 μM and MK-886 (FLAP Inh ...
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bioRxiv - Cell Biology 2020Quote: ... They were then pelleted down by centrifuging at 1000 rpm for 5 min and then washed with PBS supplemented with 10 μM MG-132 (Calbiochem). After centrifugation ...
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bioRxiv - Genetics 2021Quote: ... The larvae were individualized in each well of 96-well plates containing ATR Inhibitor IV (CAS 1232410-49-10 Calbiochem) at 10 µM or DMSO in egg water as negative control ...
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bioRxiv - Microbiology 2021Quote: Primary macrophages pre-treated with the following reagents one hour prior to infection with Mtb H37Rv: GSI (Calbiochem, 10 μM); EPZ015666 (Tocris ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were then incubated in a wash buffer consisting of 2X nuclease free SSC and 10% deionized formamide (Calbiochem 4610) for 5 minutes at room temperature ...
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bioRxiv - Molecular Biology 2019Quote: ... 0.1 mM PMSF and 0.1 μM Aprotinin) using a Dounce homogenizer (10 strokes, 400 rpm) and filtered through two layers of Mira cloth (Calbiochem). The filtrate was centrifuged at 600g at 4° C for 2 min to remove crud and unbroken cells ...
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bioRxiv - Neuroscience 2019Quote: ... Then preparations were incubated for 15 min with hybridization buffer prepared using 2x saline-sodium citrate (SSC) solution / 10 % deionized formamide (4610-OP, Calbiochem). Hybridization with Stellaris FISH probes was done in a total volume of 50 μl hybridization buffer containing 50 μg competitor tRNA from E.coli (10109541001 ...
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bioRxiv - Bioengineering 2019Quote: ... with 10% FBS media (500 ml of MEM, 2.5 g D-glucose, 100 mg NaHCO3, 50 mg transferrin (Calbiochem 616420), 50 ml FBS (heat inactivated ...
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bioRxiv - Microbiology 2021Quote: ... confluent epithelial cell monolayers were treated with the following inhibitors at concentrations previously determined (5): SP600125 (Primary target: JNK1, JNK2, JNK3; 10 μM, Calbiochem), SB203580 (Primary target ...
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bioRxiv - Cell Biology 2020Quote: ... the following inhibitors were used at the indicated concentrations for 4 h unless indicated differently in the experiments: RO-3306 at 10 μM (CDK1 inhibitor; Calbiochem), NU6140 at 10 μM (CDK2 inhibitor ...
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bioRxiv - Immunology 2021Quote: ... as a pan-caspase inhibitor or 10 µM N-acetyl-tyrosyl-valyl-alanyl-aspartyl chloromethyl ketone (Ac-YVAD-cmk, Calbiochem) as a caspase 1 inhibitor ...
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bioRxiv - Cancer Biology 2020Quote: ... slides were counterstained with DAPI (1:50000 from 10 mg/ml stock solution, Serva Eletrophoresis) and embedded with geltol (Calbiochem). 2 representative pictures of 4 biological replicates per genotype were taken with LSM 5 Exciter (Zeiss ...
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bioRxiv - Cell Biology 2021Quote: ... in PBS for 10 min and the coverslips were mounted cell side down on object glasses with Mowiol (Calbiochem, 475904) containing 50 mg/ml 1,4-diazabicyclo[2.2.2]octane (Dabco ...
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bioRxiv - Developmental Biology 2020Quote: ... was collected and transferred to new 6-well plates in the same culture medium with the addition of 10 µM of the Casp3 inhibitor (Ac-DMQD-CHO, Cat. 235421 – Calbiochem). The culture was maintained for more 20h ...
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bioRxiv - Cell Biology 2021Quote: ... the cell pellet was lysed by sonication in lysis buffer (2mM Tris–HCl pH 7.4, 0.5M NaCl, 20mM imidazole, 10% glycerol, Protease Inhibitor Cocktail Set III Calbiochem, 1:10,000) and centrifuged (10,000g ...
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bioRxiv - Immunology 2022Quote: ... Cells were left unstimulated or stimulated for indicated time at 37 °C with PMA (10 ng/ml; Alexis) and ionomycin (1 μM; Calbiochem) or solvent (DMSO) ...
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bioRxiv - Microbiology 2022Quote: Primary macrophages pre-treated with the following reagents one hour prior to infection with Mtb H37Rv: SB203580 (Calbiochem, 10 μM); SP600125 (Calbiochem ...
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bioRxiv - Molecular Biology 2022Quote: ... After 4 h the cells were starved in serum free medium overnight and then incubated for 1h with the ADAM17 inhibitor Batimastat (BB-94) at 10 μM (196440, Calbiochem), with the selective ADAM10 inhibitor GI254023X (GI ...
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bioRxiv - Neuroscience 2023Quote: ... After washing three times for 10 min in TBST the sections were mounted on glass slides using Mowiol (Calbiochem/ MerckMillipore), glass covered and stored at 4°C.
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bioRxiv - Cell Biology 2023Quote: ... the cells were fixed with cold methanol (10 min at −20°C) and blocked for 30 min with 3% (w/v) bovine serum albumin (Calbiochem) in 1 x PBS at room temperature ...
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bioRxiv - Pathology 2024Quote: ... NETs formation was induced for 3 hours at 37°C and 5% CO2 by stimulation with 10 µM of PAF (Calbiochem) on primed neutrophils and 25 µM on non-primed ones ...
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bioRxiv - Neuroscience 2024Quote: ... The NPCs were differentiated into neurons in the absence of bFGF for at least 3 weeks and treated with 10 μM ROCK inhibitor (Y-27632; Calbiochem) during the first 24 h.
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bioRxiv - Molecular Biology 2024Quote: ... the transfected cells were split and plated into three 10-cm dishes in culture medium containing selection antibiotics blasticidin S (15 μg/ml, Calbiochem) and hygromycin B (100 μg/ml ...
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bioRxiv - Cancer Biology 2020Quote: ... plate wells were washed with PBS containing 2% FBS and incubated overnight with biotinylated HA-binding protein (3 μg/ml, Calbiochem-EMD Millipore) at 4° C (34) ...
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bioRxiv - Cancer Biology 2020Quote: ... plate wells were washed with PBS containing 2% FBS and incubated overnight with biotinylated HA-binding protein (3 μg/ml, Calbiochem-EMD Millipore) at 4° C (16) ...
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bioRxiv - Cell Biology 2020Quote: ... and all cell types were lysed with Pierce™ RIPA Buffer (ThermoScientific 89900) supplemented with 1X Phosphatase Inhibitor Cocktail Set 2 (Calbiochem 5246251) and 1X Protease Inhibitor Cocktail Set 1 (Calbiochem 539131) ...
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bioRxiv - Cell Biology 2021Quote: ... plate wells were washed with PBS containing 2% FBS and incubated overnight with biotinylated HA-binding protein (3 μg/ml, Calbiochem-EMD Millipore) at 4° C (7) ...
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bioRxiv - Microbiology 2019Quote: ... or pronase (a mixture of endo- and exoproteases from Streptomyces griseus at a final concentration of 5, 10 or 50 μg/ml; Calbiochem, UK) for 30 min ...
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bioRxiv - Neuroscience 2022Quote: ... frozen neural progenitor cells (NPCs) were thawed in the neural maintenance growth medium and supplemented with ROCK inhibitor (Y-27632; 10 mM Calbiochem, #688000). Cells were incubated in a humidified chamber at 37°C with 5% CO2 ...
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bioRxiv - Biochemistry 2021Quote: ... The sVSG depleted cell ghost pellet was resuspended in 1.5 ml of 20 mM ammonium bicarbonate and mixed with 50 µl of 10 mg/ml of freshly prepared Pronase (Calbiochem, #53702) dissolved in 5 mM calcium acetate and digested at 37 °C for 24 h ...
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bioRxiv - Neuroscience 2022Quote: ... the cells were then incubated overnight at 4°C with primary antibodies (rabbit anti-AUTS2, 1:200, Atlas Antibodies; rabbit anti-GRIA1, 1:10, Calbiochem; mouse anti-GRIA2 ...
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bioRxiv - Molecular Biology 2024Quote: ... and resuspended in 1.5 mL of 20 mM ammonium bicarbonate and mixed with 50 µl of 10 mg/mL of freshly prepared Pronase (Calbiochem, #53702) dissolved in 5 mM calcium acetate and digested at 37 °C for 24 h ...
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bioRxiv - Biochemistry 2021Quote: ... PTP1B KO Ramos were transfected with a doxycycline inducible tet-on vector and exposed to 2 μg/ml doxycycline (Calbiochem, Merck, Darmstadt, Germany) 24 to 48 h before the experiment ...
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bioRxiv - Cancer Biology 2020Quote: Dissected CP specimens were dissociated using forceps under a stereoscope followed by enzymatic digestion at 37°C for 20 minutes with pronase (2 mg/ml, 537088, Calbiochem, San Diego, CA) in Hank’s balanced salt solution (HBSS ...
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bioRxiv - Molecular Biology 2023Quote: ... the cells were solubilized for 1 h at 4□°C in buffer (50□mM HEPES-NaOH [pH 7.5], 300□mM NaCl, 2% (w/v) N-dodecyl β-d-maltoside (DDM, Calbiochem, San Diego, CA), protease inhibitor cocktail ...
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bioRxiv - Cancer Biology 2023Quote: ... hatched zebrafish embryos were engrafted via the Duct of Cuvier with 1×105 cells/µL tumor cells resuspended in 2% polyvinypyrrolidone 40 (PVP-40, Calbiochem, San Diego, USA) using borosilicate glass capillary needles (1mm O.D ...
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bioRxiv - Molecular Biology 2023Quote: ... the mice were anaesthetized using 2% isoflurane and given a retro-orbital injection of 21.75 mg kg−1 body weight puromycin (Calbiochem, San Diego, CA, USA) in saline to measure muscle protein synthesis73.
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bioRxiv - Physiology 2019Quote: ... Individual gel slices were subjected to in-gel reduction with dithiothreitol (10 mM, 30 min, 56 °C, Merck-Calbiochem, San Diego, USA), alkylation with iodoacetamide (50 mM ...
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bioRxiv - Cancer Biology 2020Quote: ... dipped in MilliQ, and mounted in Mowiol (0.1 M tris-HCl pH 8.5, 25% glycerol, 10% Mowiol (Calbiochem, Merck Millipore, Darmstadt, Germany)) ...
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bioRxiv - Immunology 2021Quote: B cell staining was performed on frozen PBMCs after thawing in complete medium (RPMI 10% FBS, PS/G 1X) containing DNase I (Calbiochem, cod 260913) for 10 min at 37°C ...
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bioRxiv - Molecular Biology 2021Quote: To test for the EV nature of labelled objects detergent controls were performed by adding a sample volume of a 2% (w/v) NP-40 solution (Calbiochem, San Diego, CA, USA) to the samples.
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bioRxiv - Plant Biology 2020Quote: ... The peels were then transferred into 10-ml buffer (Araújo et al., 2011) containing the enzyme CELLULYSIN cellulase from Trichoderma viride (Calbiochem, La Jolla, CA, USA) and digested for 1 h at a shaking speed of 150 rpm ...
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bioRxiv - Cell Biology 2021Quote: ... pre-equilibrated with running buffer (PBS, 20mM imidazole, 10% glycerol, protease Inhibitor Cocktail Set III Calbiochem, Ref. 539134-1 at 1:10,000 dilution). The column was washed with five column volumes (CV ...
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bioRxiv - Microbiology 2021Quote: ... to OD600 1 were incubated with 750 μl of lysis buffer (100 mM EDTA [pH 8.0], 1 mM MgCl2, 5 μg/ml lysostaphin, 10 μg/ml DNase, proteinase inhibitor [cocktail set III from Calbiochem] diluted 1:100 in PBS) for 30 min at 37°C and homogenized three times with 500 μl zirconia-silica beads (0.1 mm diameter from Carl-Roth ...
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bioRxiv - Cancer Biology 2019Quote: ... FLAG-PITX1 stable expression clones were maintained in DMEM supplemented with 10% FBS and G418 (HeLa229 and U2OS 500 μg/ml, 293T 600 μg/ml; Calbiochem, La Jolla, CA, USA). All Cell lines detected as Mycoplasma-free by MycoAlert mycoplasma detection kit (Lonza ...
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bioRxiv - Microbiology 2019Quote: ... Changes in net electrogenic ion flux were evaluated in the ileum by measuring changes in Isc in response to neuronal depolarization with 10 µM veratridine (Calbiochem, San Diego, CA, USA) or in response to muscarinic receptor stimulation with 100 µM carbachol (Sigma) ...
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bioRxiv - Neuroscience 2020Quote: ... Bacteria were resuspended in (50mM HEPES, 250mM NaCl, 10% glycerol plus 1% Triton X-100, supplemented with protease inhibitors (Merck, Calbiochem set III, Cat. No. 539134) and subsequently sonicated ...
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bioRxiv - Cell Biology 2022Quote: ... cells were washed with ice-cold PBS and lysed with ice-cold IP buffer (10 mM HEPES pH 7.4; 150 mM NaCl, 1% Triton X-100, plus protease inhibitor cocktail Set I-Calbiochem 1:100 (Cat# 539131, Merck Millipore), 1 mM Na3VO4 and 1mM NaF) ...