Labshake search
Citations for Calbiochem :
101 - 150 of 633 citations for 7 Bromo 2 4 fluoro phenyl imidazo 1 2 a pyridine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2023Quote: ... 4.3 mM Bromphenol Blue and 5% 2-mercaptoethanol) supplemented with phosphatase (Calbiochem, 524625) and protease (Roche ...
-
bioRxiv - Cancer Biology 2023Quote: ... hatched zebrafish embryos were engrafted via the Duct of Cuvier with 1×105 cells/µL tumor cells resuspended in 2% polyvinypyrrolidone 40 (PVP-40, Calbiochem, San Diego, USA) using borosilicate glass capillary needles (1mm O.D ...
-
bioRxiv - Molecular Biology 2023Quote: ... the mice were anaesthetized using 2% isoflurane and given a retro-orbital injection of 21.75 mg kg−1 body weight puromycin (Calbiochem, San Diego, CA, USA) in saline to measure muscle protein synthesis73.
-
bioRxiv - Cell Biology 2023Quote: ... cells were treated for 2 h with 35 µM cycloheximide (Calbiochem, catalog no. 239763). For mTOR inhibition-dependent autophagy arrest at the initiation step ...
-
bioRxiv - Neuroscience 2023Quote: ... 4-Hydroxynonenal (HNE)-Michael adducts (1:2,000 Calbiochem, ref. 393207) and acetylated-lysine (1:2,000 Cell Signaling ...
-
bioRxiv - Microbiology 2021Quote: ... Cells were split roughly 1:4 and supplemented with 1 mM Rock-inhibitor (Y27632; Calbiochem). Cells were fed with new media daily.
-
bioRxiv - Microbiology 2023Quote: ... 2-day-grown conidia were collected and filtered through Miracloth (Calbiochem, San Diego, CA, USA) (66).
-
bioRxiv - Neuroscience 2023Quote: ... samples were washed in PBS and 2 drops of FluorSave reagent (Calbiochem, San Diego, California) were added to tissue sections ...
-
bioRxiv - Cell Biology 2024Quote: ... 0.1 % Triton X-100 in PBS or 2 % bovine serum albumin (GE Healthcare or Calbiochem), 0.01 % Tween 20 (National Diagnostics or Roth ...
-
bioRxiv - Immunology 2022Quote: ... Dead cell exclusion was performed using 7-AAD (Calbiochem). For intracellular cytokine staining of T cells ...
-
bioRxiv - Cell Biology 2023Quote: ... a neutralizing antibody against E-cadherin (SHE78-7, Calbiochem) was used at the final concentration of either 1 or 3 µm/ml ...
-
bioRxiv - Neuroscience 2021Quote: ... InSolution Roscovitine (CAS # 186692-46-6) and Bafilomycin A1 (CAS #88899-55-2) were from Calbiochem. N6-Benzoyladenosine-3’ ...
-
bioRxiv - Immunology 2023Quote: ... 2 µl were used as PCR template for amplification with the KOD Polymerase (Calbiochem, 71086-3). The PCR products were cloned into the pI.18 vector ...
-
bioRxiv - Cancer Biology 2021Quote: ... – supplemented with Y-27632 (10 μM; Calbiochem, 146986-50-7), as previously described64 ...
-
bioRxiv - Biophysics 2022Quote: ... [Ca2+]ER levels were assessed by the addition of 2 µM ionomycin (IO; Calbiochem, San Diego, CA), a Ca2+ ionophore ...
-
bioRxiv - Neuroscience 2021Quote: ... they were incubated for 2 h in secondary antibody (anti-mouse fluorescein conjugated, Calbiochem cat n. 401234) 1:100 in BSA 1% + sheep serum 2% in PBT ...
-
bioRxiv - Immunology 2020Quote: ... Rac1 inhibitor (NSC23766) and Arp-2 complex inhibitor I (CK-666) & II (CK-869) purchased from Calbiochem were used for blocking actin assembly (33).
-
bioRxiv - Immunology 2023Quote: ... NF-κB (BAY11-7082; 2 μM) and RAF1 (RAF1 Kinase inhibitor I; 10 μM) were from Calbiochem. The inhibitor of EGFR (gefitinib ...
-
bioRxiv - Molecular Biology 2022Quote: ... The reaction was started by adding 1 nM to 4 μM calmodulin (Calbiochem) and 100 μM ATP (∼1 Ci mmol-1 [γ32P]-ATP) ...
-
bioRxiv - Neuroscience 2020Quote: ... sections were incubated overnight at 4°C in blocking solution with anti-TH (1:2,500; Chemicon) and anti-BiP (1:1,000; Calbiochem) antibodies and detected using secondary alexa-488 anti-rabbit and alexa-564 anti-mouse antibodies ...
-
bioRxiv - Molecular Biology 2020Quote: ... 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reagent was obtained from Calbiochem (San Diego, CA, USA).
-
bioRxiv - Cell Biology 2023Quote: ... mouse organs were homogenized in 2% SDS lysis buffer containing protease inhibitor mixture III (# 535140, Calbiochem, Billerica, MA) and phosphatase inhibitors (#P2850 ...
-
bioRxiv - Cell Biology 2023Quote: ... RPE-1 cells transfected with either siR-NAs targeting Luciferase or MiniBAR for 72 h were treated with Y27632 (Calbiochem 146986-50-7) at 50 µM for 24 h in DMEM-F12 without SVF before fixation.
-
bioRxiv - Immunology 2023Quote: ... 4-iodo-6-phenylpyrimidine (4-IPP) (Calbiochem CAS 41270) was injected intraperitoneally at 5 mg/kg every 48 hours.
-
bioRxiv - Pharmacology and Toxicology 2019Quote: ... and the phenylacetamide compound (S)-2-(4-chlorophenyl)-3,3-dimethyl-N-(5-phenylthiazol-2-yl)butanamide (PA;Cmp58 (Wang et al., 2010)) was obtained from Calbiochem-Merck Millipore (Billerica ...
-
Proteomics of broad deubiquitylase inhibition unmasks redundant enzyme function to reveal substratesbioRxiv - Biochemistry 2020Quote: Interphase extract was prepared as previously described(Murray AW 1991) but using 2 μg/ml calcium ionophore (A23187, Calbiochem) for egg activation ...
-
bioRxiv - Molecular Biology 2022Quote: ... Chronic low-dose inflammation was mimicked in 3T3-L1 adipocytes by incubation with 2 ng/mL TNFα (TNF; Calbiochem) for 4 d ...
-
bioRxiv - Plant Biology 2022Quote: ... except that the resuspension of protein precipitate was in modified denaturing urea buffer (8 M urea, 2% SDS, 100 mM HEPES-KOH pH 7.4, 10 mM EDTA and protease inhibitor [Calbiochem, now Merck ...
-
bioRxiv - Physiology 2023Quote: ... Fura-2 AM and BAPTA-AM (Cat# F1221, Molecular Probes, Eugene, OR, USA and CAS# 126150-97-8, Calbiochem San Diego ...
-
bioRxiv - Cell Biology 2023Quote: ... The homogenate was then incubated on ice for 5 minutes and filtered through 2 layers of Miracloth tissue (Calbiochem). The filtered homogenate was then centrifuged for 20 min at 480 rpm at 4°C to pellet any debris ...
-
bioRxiv - Cancer Biology 2023Quote: NMDI-14 inhibitor (Ethyl 2-(((6,7-dimethyl-3-oxo-1,2,3,4-tetrahydro-2-quinoxalinyl)acetyl)amino)-4,5-dimethyl-3-thiophenecarboxylate) was purchased from Calbiochem (Cat. Nr. 530838) and reconstituted in DMSO (10mg/ml ...
-
bioRxiv - Immunology 2019Quote: ... Injection inoculum was prepared from an overnight liquid culture in the log-phase of growth resuspended in 2% polyvinylpyrrolidone40 (PVP40) solution (CalBiochem) as previously described (40) ...
-
bioRxiv - Cell Biology 2021Quote: ... Cells were arrested in S phase or at the G1/S boundary by single or double thymidine (2 mM, Calbiochem) treatment ...
-
bioRxiv - Developmental Biology 2022Quote: ... Drosophila ovaries from aPKCas4 flies (prepared as previously described in the optogenetic experiments section) were cultured ex vivo for 2 hours in the presence of the ATP analog 1NA-PP1 (Calbiochem; prepared in DMSO ...
-
bioRxiv - Physiology 2021Quote: ... or 0.2 mL (for lung) HEPES-buffered saline plus Phosphatase Inhibitor Cocktail Set II and Protease Inhibitor Cocktail Set III (Calbiochem). Protein was measured using a Pierce™ BCA protein assay kit (Thermo Fisher ...
-
bioRxiv - Molecular Biology 2021Quote: ... Fresh 2-day conidia from each strain were harvested from ANM plates in PBS solution and filtered through a Miracloth (Calbiochem). For each strain ...
-
bioRxiv - Cell Biology 2022Quote: ... the P2Y2R antagonist AR-C118925 {5-[[5-(2,8-dimethyl-5Hdibenzo[α,d]cyclohepten-5-yl)-3,4-dihydro-2-oxo-4-thioxo-1(2H)-pyrimidinyl]methyl]-A-2H-tetrazol-5-yl-2-furan-carboxamide},and the Ca2+ probes thapsigargin and ionomycin were obtained from Calbiochem-Merck Millipore (Billerica ...
-
bioRxiv - Neuroscience 2022Quote: ... the cells were then incubated overnight at 4°C with primary antibodies (rabbit anti-AUTS2, 1:200, Atlas Antibodies; rabbit anti-GRIA1, 1:10, Calbiochem; mouse anti-GRIA2 ...
-
bioRxiv - Neuroscience 2019Quote: ... the slices were treated with pronase (1 mg / 4-6 ml) (Calbiochem-MilliporeSigma, Burlington, MA, USA) in incubation medium I at 31°C for 30-50 min ...
-
bioRxiv - Cancer Biology 2020Quote: ... Blots were incubated with following primary antibodies at 4 C overnight: TP53 (OP43, Calbiochem, 1:1000), RB1 (Ab-5 ...
-
bioRxiv - Cell Biology 2024Quote: ... Membranes were incubated overnight at 4°C,with mouse anti-PGC-1α (Calbiochem, KP9803, 1:1000), mouse anti-PINK1 (Santa Cruz Biotechnology ...
-
bioRxiv - Neuroscience 2024Quote: ... Aphidicolin 4 μM (Calbiochem) was used to restrict the proliferation and viability of small numbers of non-neuronal cells.
-
bioRxiv - Cancer Biology 2020Quote: ... plate wells were washed with PBS containing 2% FBS and incubated overnight with biotinylated HA-binding protein (3 μg/ml, Calbiochem-EMD Millipore) at 4° C (34) ...
-
bioRxiv - Cancer Biology 2020Quote: ... plate wells were washed with PBS containing 2% FBS and incubated overnight with biotinylated HA-binding protein (3 μg/ml, Calbiochem-EMD Millipore) at 4° C (16) ...
-
bioRxiv - Cell Biology 2020Quote: ... and all cell types were lysed with Pierce™ RIPA Buffer (ThermoScientific 89900) supplemented with 1X Phosphatase Inhibitor Cocktail Set 2 (Calbiochem 5246251) and 1X Protease Inhibitor Cocktail Set 1 (Calbiochem 539131) ...
-
bioRxiv - Cell Biology 2021Quote: ... plate wells were washed with PBS containing 2% FBS and incubated overnight with biotinylated HA-binding protein (3 μg/ml, Calbiochem-EMD Millipore) at 4° C (7) ...
-
bioRxiv - Biochemistry 2020Quote: ... 5-8×106 cell pellets were washed twice and resuspended in cold DPBS and incubated on ice for 10 min with 2 mg/mL digitonin (Calbiochem, #3000410-5GM). Next ...
-
bioRxiv - Immunology 2021Quote: ... RAW264.7 and Huh-7 cells were stimulated with LPS from Escherichia coli O111:B4 (Calbiochem).
-
bioRxiv - Biochemistry 2021Quote: ... PTP1B KO Ramos were transfected with a doxycycline inducible tet-on vector and exposed to 2 μg/ml doxycycline (Calbiochem, Merck, Darmstadt, Germany) 24 to 48 h before the experiment ...
-
bioRxiv - Cancer Biology 2020Quote: Dissected CP specimens were dissociated using forceps under a stereoscope followed by enzymatic digestion at 37°C for 20 minutes with pronase (2 mg/ml, 537088, Calbiochem, San Diego, CA) in Hank’s balanced salt solution (HBSS ...