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Citations for Calbiochem :
151 - 200 of 207 citations for 6H Pyrano 3 2 g benzothiazole 8CI 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... 4.3 mM Bromphenol Blue and 5% 2-mercaptoethanol) supplemented with phosphatase (Calbiochem, 524625) and protease (Roche ...
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bioRxiv - Developmental Biology 2021Quote: ... transferred to a 15 ml centrifuge tube containing warm 3 ml 0.5 mg ml−1 collagenase type II (Calbiochem, 234155) solution and incubated for 30 min at 37°C shaker at 180 rpm ...
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bioRxiv - Immunology 2019Quote: ... Sections were then incubated overnight in (4°C) with anti-c-ErbB2/c-Neu (Ab-3) mouse mAb (3B5) (1:500, Calbiochem). After washing with PBS ...
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bioRxiv - Immunology 2019Quote: ... bovine serum albumin in 0.1 M phosphate buffer for 1 h at room temperature and incubated overnight (4°C) with anti-c-ErbB2/c-Neu (Ab-3) mouse mAb (3B5) (1:500, Calbiochem) or anti-CD3 (1:500 ...
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bioRxiv - Molecular Biology 2021Quote: ... The expression of recombinant PGC-1α proteins was induced for 3 h at 37°C with 0.4 mM isopropyl-β-D-thiogalactoside (IPTG, Calbiochem) in Terrific Broth and purified by metal ion affinity chromatography (IMAC ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were washed 3 times with PBS and fixed to a slide using 5 μL of Mowiol (4-88 Reagent, Calbiochem). Fluorescent images were acquired with a Leica Dmi8 microscope (Leica Microsystems).
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bioRxiv - Cell Biology 2023Quote: ... the cells were fixed with cold methanol (10 min at −20°C) and blocked for 30 min with 3% (w/v) bovine serum albumin (Calbiochem) in 1 x PBS at room temperature ...
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bioRxiv - Pathology 2024Quote: ... NETs formation was induced for 3 hours at 37°C and 5% CO2 by stimulation with 10 µM of PAF (Calbiochem) on primed neutrophils and 25 µM on non-primed ones ...
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bioRxiv - Neuroscience 2024Quote: ... The NPCs were differentiated into neurons in the absence of bFGF for at least 3 weeks and treated with 10 μM ROCK inhibitor (Y-27632; Calbiochem) during the first 24 h.
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bioRxiv - Cell Biology 2023Quote: ... cells were treated for 2 h with 35 µM cycloheximide (Calbiochem, catalog no. 239763). For mTOR inhibition-dependent autophagy arrest at the initiation step ...
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bioRxiv - Microbiology 2022Quote: ... the preformed spheroids were transferred into the fibrin gel consisting of plasminogen-free human fibrinogen (final concentration 3 mg/ml; Calbiochem, USA) and human thrombin (final concentration 2 U/ml ...
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bioRxiv - Cell Biology 2022Quote: ... wild-type mouse testes (n=3 per IP assay) were lysed in 1% NP-40 (PBS) supplemented with protease inhibitor cocktail (Calbiochem, 539134). KATNA1 ...
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bioRxiv - Microbiology 2023Quote: ... 2-day-grown conidia were collected and filtered through Miracloth (Calbiochem, San Diego, CA, USA) (66).
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bioRxiv - Neuroscience 2023Quote: ... samples were washed in PBS and 2 drops of FluorSave reagent (Calbiochem, San Diego, California) were added to tissue sections ...
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bioRxiv - Cell Biology 2024Quote: ... 0.1 % Triton X-100 in PBS or 2 % bovine serum albumin (GE Healthcare or Calbiochem), 0.01 % Tween 20 (National Diagnostics or Roth ...
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bioRxiv - Cancer Biology 2019Quote: ... Membranes were incubated in 1:1000 diluted primary antibodies pERK1/2 pT202/Y204 (Calbiochem cat# KP26001), total ERK1/2 (Cell Signaling cat# 9102) ...
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bioRxiv - Neuroscience 2021Quote: ... InSolution Roscovitine (CAS # 186692-46-6) and Bafilomycin A1 (CAS #88899-55-2) were from Calbiochem. N6-Benzoyladenosine-3’ ...
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bioRxiv - Biophysics 2022Quote: The activity of β-galactosidase was measured using a spectrophotometric assay in which o-Nitrophenyl-β-D-Galactopyranoside (ONPG, CAS Nr: 369-07-3 Calbiochem Sigma-Aldrich) was used as a substrate ...
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bioRxiv - Molecular Biology 2020Quote: ... Pellets were resuspended in 300 µl of DMEM 2% FBS 1 µg/mL propidium iodide (Calbiochem, 537060) and filtered through a 40-µm cell strainer (BD Falcon ...
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bioRxiv - Biophysics 2022Quote: ... [Ca2+]ER levels were assessed by the addition of 2 µM ionomycin (IO; Calbiochem, San Diego, CA), a Ca2+ ionophore ...
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bioRxiv - Molecular Biology 2020Quote: ... Lyophilized MEISi-1 and MEISi-2 small molecules were dissolved in sterile dimethyl sulfoxide (DMSO, Calbiochem, 317275). Luciferase reporter assays were carried out as we have done previously 13,14,16,17 ...
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bioRxiv - Neuroscience 2021Quote: ... they were incubated for 2 h in secondary antibody (anti-mouse fluorescein conjugated, Calbiochem cat n. 401234) 1:100 in BSA 1% + sheep serum 2% in PBT ...
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bioRxiv - Immunology 2020Quote: ... Rac1 inhibitor (NSC23766) and Arp-2 complex inhibitor I (CK-666) & II (CK-869) purchased from Calbiochem were used for blocking actin assembly (33).
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bioRxiv - Neuroscience 2023Quote: Cortical neurons were incubated with 4 μM fura-2 acetoxymethyl ester (AM, Calbiochem, CAS 108964-32-5) 40 min at 37°C ...
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bioRxiv - Immunology 2023Quote: ... NF-κB (BAY11-7082; 2 μM) and RAF1 (RAF1 Kinase inhibitor I; 10 μM) were from Calbiochem. The inhibitor of EGFR (gefitinib ...
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bioRxiv - Physiology 2021Quote: ... and phosphatase inhibitors (2 μM microcystin-LR, and 1× Phosphatase-Inhibitor Sets I and II (Calbiochem, Hayward, CA). We centrifuged homogenates at 4,000 g for 15 minutes to remove debris ...
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bioRxiv - Pathology 2023Quote: ... 2% DDM) supplemented with 1:1000 (v/v) Protease Inhibitor Cocktail Set III (Calbiochem, La Jolla, CA, USA). Rat anti-HA (3F10 ...
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bioRxiv - Cell Biology 2023Quote: ... mouse organs were homogenized in 2% SDS lysis buffer containing protease inhibitor mixture III (# 535140, Calbiochem, Billerica, MA) and phosphatase inhibitors (#P2850 ...
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bioRxiv - Cancer Biology 2021Quote: ... and 2 mM glutamine for 24 h prior to treatment with either TGF-β (1 ng/mL, Millipore Calbiochem) or 10 uM TGF-β inhibitors (SB431542 ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... and the phenylacetamide compound (S)-2-(4-chlorophenyl)-3,3-dimethyl-N-(5-phenylthiazol-2-yl)butanamide (PA;Cmp58 (Wang et al., 2010)) was obtained from Calbiochem-Merck Millipore (Billerica ...
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Proteomics of broad deubiquitylase inhibition unmasks redundant enzyme function to reveal substratesbioRxiv - Biochemistry 2020Quote: Interphase extract was prepared as previously described(Murray AW 1991) but using 2 μg/ml calcium ionophore (A23187, Calbiochem) for egg activation ...
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bioRxiv - Physiology 2021Quote: ... cells were treated with poly(I:C) (1.5 μg/mL for 4 hrs) and a combination of proteasome inhibitor (Bortezomib, 2 μM, Sigma/Calbiochem 5043140001) and prolyl endopeptidase inhibitors (Pramiracetam 10 uM ...
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bioRxiv - Molecular Biology 2022Quote: ... Chronic low-dose inflammation was mimicked in 3T3-L1 adipocytes by incubation with 2 ng/mL TNFα (TNF; Calbiochem) for 4 d ...
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bioRxiv - Plant Biology 2022Quote: ... except that the resuspension of protein precipitate was in modified denaturing urea buffer (8 M urea, 2% SDS, 100 mM HEPES-KOH pH 7.4, 10 mM EDTA and protease inhibitor [Calbiochem, now Merck ...
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bioRxiv - Microbiology 2022Quote: ... two reactions with increasing dosages of the PfCKII-α-specific inhibitors 4,5,6,7-tetramethylbromobenzotriazole and 4,5,6,7-tetramethylbromo-2-azabenzimidazole (TBB) (Merck/Calbiochem CAS 17374-26-4) were performed to check for possible suppression of phosphoryl group transfer ...
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bioRxiv - Microbiology 2023Quote: ... Plates were then incubated at 37 °C for 2 h with different quantities (0.125 - 4 pmol) of biotinylated PfPP1c labeled with biotin-NHS (Calbiochem) in PBS-Tween 0.1% ...
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bioRxiv - Physiology 2023Quote: ... Fura-2 AM and BAPTA-AM (Cat# F1221, Molecular Probes, Eugene, OR, USA and CAS# 126150-97-8, Calbiochem San Diego ...
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bioRxiv - Biochemistry 2023Quote: ... Ubiquitin was expressed as described above except that the 2 L culture was induced with 0.4 mM dioxane-free isopropyl B-D-1-thiogalactopyranoside (IPTG) (Calbiochem) for four hrs at 37℃ ...
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bioRxiv - Cell Biology 2023Quote: The antibodies listed in Tables 1 and 2 were diluted as indicated in PBS containing 1% BSA (126575, Calbiochem).
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bioRxiv - Cell Biology 2023Quote: ... The homogenate was then incubated on ice for 5 minutes and filtered through 2 layers of Miracloth tissue (Calbiochem). The filtered homogenate was then centrifuged for 20 min at 480 rpm at 4°C to pellet any debris ...
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bioRxiv - Immunology 2021Quote: ... GMDCs were incubated for 2 h at 37°C with 50 µM MARK inhibitor (MARK/Par-1 Activity Inhibitor, 39621; Calbiochem), 50 nM SIK inhibitor (HG-9-91-01 ...
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bioRxiv - Immunology 2019Quote: ... Injection inoculum was prepared from an overnight liquid culture in the log-phase of growth resuspended in 2% polyvinylpyrrolidone40 (PVP40) solution (CalBiochem) as previously described (40) ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were arrested in S phase or at the G1/S boundary by single or double thymidine (2 mM, Calbiochem) treatment ...
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bioRxiv - Neuroscience 2021Quote: Pharmacological agents were used in the following concentrations and time courses: cycloheximide (Calbiochem, 0.2 μg/μl, 2, 4 or 8h), dexamethasone (Invivogen ...
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bioRxiv - Developmental Biology 2022Quote: ... Drosophila ovaries from aPKCas4 flies (prepared as previously described in the optogenetic experiments section) were cultured ex vivo for 2 hours in the presence of the ATP analog 1NA-PP1 (Calbiochem; prepared in DMSO ...
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bioRxiv - Cell Biology 2019Quote: ... Crosses were performed by random spore analysis (1, 2) followed by marker selection (Hygromycin/Kanamycin resistance, ura/leu auxotrophy, or sensitivity to 5 µM 1NM-PP1 (Calbiochem) for strains carrying the Ark1-as3 allele ...
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bioRxiv - Physiology 2021Quote: ... or 0.2 mL (for lung) HEPES-buffered saline plus Phosphatase Inhibitor Cocktail Set II and Protease Inhibitor Cocktail Set III (Calbiochem). Protein was measured using a Pierce™ BCA protein assay kit (Thermo Fisher ...
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bioRxiv - Molecular Biology 2021Quote: ... Fresh 2-day conidia from each strain were harvested from ANM plates in PBS solution and filtered through a Miracloth (Calbiochem). For each strain ...
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bioRxiv - Cell Biology 2022Quote: ... the P2Y2R antagonist AR-C118925 {5-[[5-(2,8-dimethyl-5Hdibenzo[α,d]cyclohepten-5-yl)-3,4-dihydro-2-oxo-4-thioxo-1(2H)-pyrimidinyl]methyl]-A-2H-tetrazol-5-yl-2-furan-carboxamide},and the Ca2+ probes thapsigargin and ionomycin were obtained from Calbiochem-Merck Millipore (Billerica ...
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bioRxiv - Cell Biology 2020Quote: ... and all cell types were lysed with Pierce™ RIPA Buffer (ThermoScientific 89900) supplemented with 1X Phosphatase Inhibitor Cocktail Set 2 (Calbiochem 5246251) and 1X Protease Inhibitor Cocktail Set 1 (Calbiochem 539131) ...