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Citations for Calbiochem :
301 - 345 of 345 citations for 6 hydroxy 4 6 dihydrofuro 3 2 c pyran 2 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2022Quote: ... the slides were washed with PBS and mounted with water based Mowiol 4-88 (Calbiochem) mounting media ...
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bioRxiv - Genomics 2023Quote: ... and immunoprecipitations were carried out by adding 4 μg of the appropriate antibodies (MPO, Calbiochem) to
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bioRxiv - Biochemistry 2023Quote: ... The coverslips were then mounted in Mowiol 4-88 solution (Calbiochem/EMD Millipore, Billerica, MA) and fluorescence analysis was performed using a Plan-Apochromat 63×/1.4 objective (Zeiss ...
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bioRxiv - Genomics 2024Quote: ... Sections were counterstained with 0.25 µg/mL DAPI and mounted in Mowiol 4-88 (Calbiochem). Imaging was performed using the Nikon SMZ18 stereomicroscope and Olympus FluoView FV3000 confocal microscope ...
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bioRxiv - Microbiology 2023Quote: ... cells were pretreated 1 h at 37°C with 20 nM bafilomycin A1 (Calbiochem, stock in DMSO) to block endocytosis ...
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bioRxiv - Microbiology 2023Quote: ... The reaction mixture contains 8 μg total proteins and 4 μg nitrocefin (Calbiochem, San Diego, CA). β-Lactamase activity was determined by measuring the absorbance at 486 nm (OD486nm).
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bioRxiv - Cell Biology 2023Quote: ... in PBS for 5 min at RT and cells were mounted in Mowiol 4-88 (Calbiochem) solution (Wei and Seemann ...
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bioRxiv - Immunology 2020Quote: ... Cells were grown at 37°C to OD = 0.8 and expression was induced with 0.3 mM IPTG (Isopropyl b-D-1-thiogalactopyranoside, Calbiochem) overnight at 18°C ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... sections were incubated with rabbit anti-c-Fos antibody diluted with the blocking solution (1:20000, #PC38, Calbiochem) for 2 days at 4°C ...
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bioRxiv - Biophysics 2023Quote: ... and the C-class iPSCs with a mixture of two inhibitors (2i): 1 μM MEK inhibitor (PD0325901, Calbiochem) and 3 μM GSK3 inhibitor (CH99021 ...
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bioRxiv - Neuroscience 2022Quote: ... 1.33 mM Ca2+ and 1 mM of the cAMP phosphodiesterase inhibitor 3-isobutyl-1-methylxanthine (IBMX: Calbiochem, Damstard, Germany) were added to the synaptosomes for 15 min ...
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bioRxiv - Cancer Biology 2022Quote: ... The viability of the cells was then evaluated by incubating at 37 °C for 2.5 h with 200 mg/ml MTT (Calbiochem). The MTT solution was removed afterwards ...
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bioRxiv - Immunology 2022Quote: ... and 1 × 106 cells were treated with rapamycin (10 ng/ml, 30 min, 37°C; Calbiochem, San Diego, CA) prior to treatment with rhGH.
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bioRxiv - Developmental Biology 2021Quote: ... transferred to a 15 ml centrifuge tube containing warm 3 ml 0.5 mg ml−1 collagenase type II (Calbiochem, 234155) solution and incubated for 30 min at 37°C shaker at 180 rpm ...
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bioRxiv - Neuroscience 2024Quote: ... The NPCs were differentiated into neurons in the absence of bFGF for at least 3 weeks and treated with 10 μM ROCK inhibitor (Y-27632; Calbiochem) during the first 24 h.
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bioRxiv - Physiology 2023Quote: Total protein extracts were obtained from microalgal cells in exponential phase of growth (e.g. 4 days) in 50 mM Tris buffer pH 8.0 supplemented with protein inhibitor cocktail (539131, Calbiochem). Free-living and symbiotic microalgae were disrupted by bead beating with a Precellys device (Bertin Technologies ...
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bioRxiv - Cell Biology 2019Quote: ... and Scott 2007) stably expressing ARL13B C-terminally tagged with GFP were generated by lentiviral infection followed by puromycin (Calbiochem) selection.
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bioRxiv - Molecular Biology 2021Quote: BL21-RP cells co-transformed with pGEX4T1/GST-NXF1 and pET9a/p15 were induced overnight at 18°C with 0.4 mM isopropyl-β-D-thiogalactoside (IPTG, Calbiochem) in Terrific Broth ...
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bioRxiv - Immunology 2022Quote: ... Cells were left unstimulated or stimulated for indicated time at 37 °C with PMA (10 ng/ml; Alexis) and ionomycin (1 μM; Calbiochem) or solvent (DMSO) ...
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bioRxiv - Microbiology 2022Quote: ... the preformed spheroids were transferred into the fibrin gel consisting of plasminogen-free human fibrinogen (final concentration 3 mg/ml; Calbiochem, USA) and human thrombin (final concentration 2 U/ml ...
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bioRxiv - Cell Biology 2022Quote: ... wild-type mouse testes (n=3 per IP assay) were lysed in 1% NP-40 (PBS) supplemented with protease inhibitor cocktail (Calbiochem, 539134). KATNA1 ...
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bioRxiv - Immunology 2021Quote: ... under the CD11c/Itgax promoter upon intraperitoneal (i.p.) injection of 4 ng/g of mouse body weight of diphtheria toxin (DT, Calbiochem) 12 hrs prior to the targeted CD11c+ cell depletion time as indicated in the relevant figure legends ...
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bioRxiv - Biochemistry 2021Quote: ... cells were trypsinized and seeded for selection in complete media supplemented with 4 μg/mL puromycin (Calbiochem, Merck Millipore). All cell lines were passaged 3 times before use and kept in culture supplemented with 2 μg/mL puromycin and/or stored in liquid nitrogen.
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... free-floating sections were washed with KPBS to remove the cryoprotectant solution and then incubated directly with polyclonal rabbit anti-c-Fos (PC-38; Calbiochem-Millipore) at 1:20000 in 0.5% Blocking Reagent (Roche ...
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bioRxiv - Biophysics 2020Quote: L-asparaginase samples were diluted to a final concentration of 280 μM and incubated at 25°C with 0.9 μg/ml of Proteinase K (Calbiochem, Cat# 539480) in the absence or presence of a 10-fold excess of L-Asp or L-Glu (2.8 mM) ...
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bioRxiv - Neuroscience 2019Quote: ... we incubated live DIV21 hippocampal cultures infected with control or UPF2 shRNAs at 37°C for 5 min with GluR1 antibody (Calbiochem, PC246) to allow labeling of surface GluR1 ...
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bioRxiv - Neuroscience 2021Quote: One series of sections was washed with KPBS to remove the cryoprotectant solution and then incubated with polyclonal rabbit anti-c-Fos (PC-38; Calbiochem-Millipore) at 1:20000 in 0.5% Blocking Reagent (Roche ...
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bioRxiv - Physiology 2019Quote: ... Individual gel slices were subjected to in-gel reduction with dithiothreitol (10 mM, 30 min, 56 °C, Merck-Calbiochem, San Diego, USA), alkylation with iodoacetamide (50 mM ...
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bioRxiv - Neuroscience 2019Quote: ... or both hippocampi from a single mouse was homogenized in 2 mL of ice-cold homogenization buffer (0.32 M sucrose, 4 mM HEPES pH 7.4, protease inhibitor cocktail EGTA-free (Calbiochem, 1:1000), RNasin (Promega ...
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bioRxiv - Immunology 2021Quote: The caspase-1 activity was determined with the fluorometric substrate Z-YVAD 7-Amido-4-trifluoromethylcoumarin (Z-YVAD-AFC, caspase-1 substrate VI, Calbiochem), as described previously (Angosto et al. ...
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bioRxiv - Cell Biology 2020Quote: ... the following inhibitors were used at the indicated concentrations for 4 h unless indicated differently in the experiments: RO-3306 at 10 μM (CDK1 inhibitor; Calbiochem), NU6140 at 10 μM (CDK2 inhibitor ...
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bioRxiv - Immunology 2022Quote: The caspase-1 activity was determined with the fluorometric substrate Z-YVAD 7-Amido-4-trifluoromethylcoumarin (Z-YVAD-AFC, caspase-1 substrate VI, Calbiochem), as described previously (Angosto et al. ...
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bioRxiv - Microbiology 2021Quote: ... parasites were fixed in 4% paraformaldehyde containing 0.0075% glutaraldehyde (Nacalai Tesque, Japan) and permeabilized with PBS containing 0.1% Triton-X100 (Calbiochem, CA, USA), then blocked with PBS containing 3% BSA ...
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bioRxiv - Biochemistry 2021Quote: ... resuspended in 4 ml ice-cold KHM buffer supplemented with 80 μg/ml high purity digitonin (Calbiochem, cat. number 300410) and incubated on ice for 5 min to permeabilise the plasma membrane ...
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bioRxiv - Immunology 2022Quote: Caspase-1 activity was determined with the fluorometric substrate Z-YVAD 7-Amido-4-trifluoromethylcoumarin (Z-YVAD-AFC, caspase-1 substrate VI, Calbiochem) as described previously [47,48] ...
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bioRxiv - Neuroscience 2023Quote: ... O-GlcNAc transferase and O-GlcNAcase activities were blocked by 5 µM OSMI-4 (MedChem Express) and 5 µM Thiamet-G (Calbiochem) treatments ...
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bioRxiv - Biochemistry 2022Quote: ... Nuclei were stained with DAPI (1:1000 in PBS, AppliChem, Darmstadt, Germany) and coverslips were mounted on Mowiol 4-88 (Calbiochem, Merck Millipore ...
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bioRxiv - Cancer Biology 2023Quote: ... The concentration of anti-E-A14-specific Ig in plasma was estimated in comparison with a standard curve generated with anti-Her2 monoclonal antibody (Ab-4; Calbiochem).
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bioRxiv - Biophysics 2022Quote: The activity of β-galactosidase was measured using a spectrophotometric assay in which o-Nitrophenyl-β-D-Galactopyranoside (ONPG, CAS Nr: 369-07-3 Calbiochem Sigma-Aldrich) was used as a substrate ...
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bioRxiv - Neuroscience 2021Quote: ... cells were rinsed two times with pre-warmed medium and incubated for 30 min at 37°C with rabbit anti-GluA1 (1/150; Calbiochem/Millipore, RRID: AB_564636) in culture medium ...
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bioRxiv - Immunology 2022Quote: ... at a 4:1 molar ratio of monomer:streptavidin in the presence of a 1x protease inhibitor cocktail solution (Calbiochem, Millipore Sigma). 1/5th volumes of streptavidin-PE or streptavidin-BV421 were added to each monomer every 20 minutes and incubated ...
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bioRxiv - Immunology 2023Quote: ... at a 4:1 molar ratio of monomer:streptavidin in the presence of a 1x protease inhibitor cocktail solution (Calbiochem, Millipore Sigma). Briefly ...
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bioRxiv - Immunology 2023Quote: ... cells were stimulated ex vivo with PMA (10ng/ml) and ionomycin (1μM) for 4 hours in the presence of 2μM monensin (Calbiochem, San Diego, CA) during the last 2 hours of stimulation ...
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bioRxiv - Cell Biology 2020Quote: Proteasome activity was quantitatively assessed in H4 cell extracts using the β5-selective fluorogenic substrate succinyl-leucine-leucine-valine-tyrosine-4-methyl-7-courmarylamide (Suc-LLVY-AMC, Calbiochem, Burlington, MA, USA) using an adapted protocol 87 ...
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bioRxiv - Neuroscience 2020Quote: ... At 4 hours post fertilisation (hpf) embryos were treated with 100µM DAPT (In solution™ γ-secretase inhibitor IX, Calbiochem, San Diego, CA, USA) in 1% DMSO in E3 medium ...