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Citations for Calbiochem :
651 - 700 of 700 citations for 6 OXO 1 2 DIHYDRO 6H PYRROLO 3 2 1 IJ QUINOLINE 5 CARBOXYLIC ACID since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2023Quote: ... Raw values were normalized by geometric mean and used for subsequent housekeeping normalization (α-tubulin values; 1:10,000 mouse anti-tubulin; Calbiochem CP06) from the same blot.
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bioRxiv - Physiology 2023Quote: ... MLEC were incubated with appropriate fluorophore-labelled secondary antibodies for 1 hour and mounted with Mowiol (Calbiochem, San Diego, CA, USA). Nuclei were visualized by DAPI staining ...
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bioRxiv - Molecular Biology 2023Quote: ... 0.1 mM EGTA, 1% Triton X-100, 0.1% sodium deoxycholate, 0.5% SLS, 0.1% SDS, 0.5 mM PMSF, Calbiochem Complete Protease Inhibitor Cocktail V). The suspension was incubated for 30 min at 4°C and then sonicated with Covaris ME220 focused-ultrasonicator (50 alternating 15-s ON and 45-s OFF intervals ...
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bioRxiv - Molecular Biology 2021Quote: ... The expression of recombinant PGC-1α proteins was induced for 3 h at 37°C with 0.4 mM isopropyl-β-D-thiogalactoside (IPTG, Calbiochem) in Terrific Broth and purified by metal ion affinity chromatography (IMAC ...
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bioRxiv - Cell Biology 2023Quote: ... the cells were fixed with cold methanol (10 min at −20°C) and blocked for 30 min with 3% (w/v) bovine serum albumin (Calbiochem) in 1 x PBS at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... The NPCs were differentiated into neurons in the absence of bFGF for at least 3 weeks and treated with 10 μM ROCK inhibitor (Y-27632; Calbiochem) during the first 24 h.
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bioRxiv - Neuroscience 2019Quote: Pre-sorted synaptosomes (~750 μl) on iced were mixed with ice cold PBS containing protease inhibitor cocktail EGTA-free (Calbiochem, 1:1000) and RNasin (Promega ...
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bioRxiv - Bioengineering 2020Quote: ... Tested additives were added to the qRT-PCR reaction mix at the following concentrations: 1% w/v Tween-20 (Calbiochem 655204-100mL), 0.8 µg/µL BSA (Fisher BP675-1) ...
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bioRxiv - Physiology 2023Quote: ... Normal goat serum was used as a blocking agent and slides were incubated in biotinylated hyaluronan binding protein (HABP, 1:500, CalBiochem, Burlington, MA) for one hour ...
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bioRxiv - Neuroscience 2020Quote: ... 5 μg/ml RNAse A pH 7.4 and protease inhibitor cocktail set III EDTA-free (Calbiochem) added of 1 mM PMSF ...
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bioRxiv - Plant Biology 2021Quote: ... and the supernatant re-centrifuged for 5 min and filtered through a layer of Miracloth (Calbiochem). Clarified extracts were incubated with pre-washed GFP-Trap®_M magnetic beads on an end-to-end tube rotator at 4°C for 1 hr ...
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bioRxiv - Plant Biology 2021Quote: ... 5-day-old seedlings were transferred to medium supplemented with 0.6 mg/L bleomycin sulphate (Calbiochem) for 24 h.
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bioRxiv - Plant Biology 2019Quote: ... 35 μL water and 5 μL 100 mM monobromobimane in acetonitrile (Thiolyte® MB, Calbiochem, www.merckmillipore.com) were mixed and added to the samples ...
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bioRxiv - Immunology 2019Quote: ... cultured in R10 for several days and activated with 5 μg/ml phytohaemagglutinin (PHA-L, Calbiochem, Millipore Sigma ...
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bioRxiv - Cell Biology 2023Quote: ... in PBS for 5 min at RT and cells were mounted in Mowiol 4-88 (Calbiochem) solution (Wei and Seemann ...
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bioRxiv - Microbiology 2022Quote: ... the preformed spheroids were transferred into the fibrin gel consisting of plasminogen-free human fibrinogen (final concentration 3 mg/ml; Calbiochem, USA) and human thrombin (final concentration 2 U/ml ...
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bioRxiv - Neuroscience 2021Quote: ... cells were rinsed two times with pre-warmed medium and incubated for 30 min at 37°C with rabbit anti-GluA1 (1/150; Calbiochem/Millipore, RRID: AB_564636) in culture medium ...
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bioRxiv - Cell Biology 2021Quote: ... NaCl-HEPES buffer with antibodies but without uEV sample as well as stained sample supplemented with 1% NP40 (Calbiochem, San Diego, CA, USA) were analyzed as controls ...
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bioRxiv - Cell Biology 2023Quote: ... RPE-1 cells transfected with either siR-NAs targeting Luciferase or MiniBAR for 72 h were treated with Y27632 (Calbiochem 146986-50-7) at 50 µM for 24 h in DMEM-F12 without SVF before fixation.
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bioRxiv - Cancer Biology 2022Quote: ... incubated with 30 nM DAPI for 5 minutes and mounted on slides with FluorSave reagent (Calbiochem 345789). Immunofluorescence images were taken using a Keyence BZ-X800E Fluorescent Microscope with a 40X or 60X objective lens ...
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bioRxiv - Biochemistry 2021Quote: ... whereupon the final two wash steps were replaced with a single wash in 1.0 ml of 5 mM sodium phosphate buffer (pH 7.4) containing 0.1% (w/v) sarkosyl (Calbiochem) and 0.3% (w/v ...
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bioRxiv - Biochemistry 2021Quote: Uridine-5-triphosphate (UTP; cat #6701) and phorbol 12,13-dibutyrate (PDBu; cat #524390) were purchased from Calbiochem. Calyculin A (cat #9902 ...
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bioRxiv - Microbiology 2021Quote: ... The conidiospores were washed by 5 mL distilled water and filtrated through two layers of Miracloth (Calbiochem, Germany). The yield of spores was measured by a hemocytometer ...
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bioRxiv - Biophysics 2022Quote: The activity of β-galactosidase was measured using a spectrophotometric assay in which o-Nitrophenyl-β-D-Galactopyranoside (ONPG, CAS Nr: 369-07-3 Calbiochem Sigma-Aldrich) was used as a substrate ...
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bioRxiv - Neuroscience 2020Quote: ... Bacteria were resuspended in (50mM HEPES, 250mM NaCl, 10% glycerol plus 1% Triton X-100, supplemented with protease inhibitors (Merck, Calbiochem set III, Cat. No. 539134) and subsequently sonicated ...
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bioRxiv - Neuroscience 2020Quote: ... Cell lysis of all samples was performed after two washes with ice-cold PBS by incubating cells for 20 min in ice-cold 1% Triton X100 in PBS with protease inhibitor cocktails (Merck, Calbiochem set III, Cat. No. 539134), subsequent thorough scraping and sonication.
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bioRxiv - Pathology 2022Quote: ... and a mouse monoclonal antibody raised against Simian Virus-40 (SV-40, Macaca mulatta polyomavirus-1, Betapolyomavirus macacae) LT antigen (CalBiochem®, Clone PAb416, Sigma-Aldrich) was used as primary antibody at a 1:200 dilution ...
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bioRxiv - Cell Biology 2022Quote: ... or kinase domain ERK3 in kinase buffer (250 mM HEPES pH 7.5, 500 mM NaCl, 25 mM MgCl2, supplemented with protease inhibitor cocktail Set I-Calbiochem 1:100 (Cat# 539131, Merck Millipore) and phosphatase inhibitors ...
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bioRxiv - Cancer Biology 2021Quote: Cell pellets were sonicated in ice-cold lysis buffer (containing 20 mM Tris-HCl, pH 7.4, 5 mM EDTA, 10 mM Na4P2O7 (Calbiochem), 100 mM NaF ...
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bioRxiv - Neuroscience 2019Quote: ... Membranes were blocked to reduce nonspecific binding using 5% milk with PBS-TWEEN® tablets (PBS-T) (Calbiochem, USA) at room temperature (RT ...
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bioRxiv - Immunology 2020Quote: ... Fixed cells were washed three times in PBS and permeabilized by incubating them in PBS containing 5% non-fat milk and 0.05% saponin (Calbiochem). Coverslips were placed in primary antibody for 1 hour then washed 3x in PBS and placed in secondary antibody ...
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bioRxiv - Neuroscience 2023Quote: ... and counterstained with DAPI (10 min) before being washed (3x 5-min PBS 0.1M) and mounted on gelatin slides and coated with FluorSave (Calbiochem). Images of the whole section were taken using Axioscan.Z1 (Zeiss ...
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bioRxiv - Cell Biology 2020Quote: ... They were then pelleted down by centrifuging at 1000 rpm for 5 min and then washed with PBS supplemented with 10 μM MG-132 (Calbiochem). After centrifugation ...
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bioRxiv - Molecular Biology 2019Quote: ... 0.1 mM PMSF and 0.1 μM Aprotinin) using a Dounce homogenizer (5 strokes, 300 rpm) and filtered through two layers of Mira cloth (Catalog# 475855, Calbiochem). The filtrate was centrifuged at 200g at 4° C for 2 min to remove crud and unbroken cells ...
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bioRxiv - Plant Biology 2019Quote: ... 35 μL water were added and the GSH was derivatised using 5 μL of 0.1 M monobromobimane (Thiolyte® MB, Calbiochem) in darkness for 15 min at 37°C ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were washed twice with 3 ml PBS at 400 g for 5 minutes at 4°C and 500 uL PI staining buffer was added (1X PBS, 50 μg/mL PI (Calbiochem), 2μg/mL RNase A (Sigma ...
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bioRxiv - Microbiology 2021Quote: ... confluent epithelial cell monolayers were treated with the following inhibitors at concentrations previously determined (5): SP600125 (Primary target: JNK1, JNK2, JNK3; 10 μM, Calbiochem), SB203580 (Primary target ...
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bioRxiv - Cancer Biology 2020Quote: ... After saturation with TBS Tween 5% BSA for 1h and hybridization of the membranes with primary overnight (BCR-ABL Ab3 Calbiochem, pCrkL Tyr207 Cell Signaling ...
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bioRxiv - Neuroscience 2020Quote: ... 4×106 cells were transferred to one well of a 6-well plate and kept in suspension under rotation (95 rpm) for 24 hours with 5 μM of ROCK inhibitor (Y-27632; Calbiochem). Forty-eight hours later ...
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bioRxiv - Cell Biology 2022Quote: DLD1-Nup153AID cells were plated at 140K per well on glass coverslips and cultured for 8 hours in the absence or presence of 5 mM thymidine (Calbiochem). 1 mM Indole-3-acetic acid (IAA ...
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bioRxiv - Systems Biology 2021Quote: Gsp1 variants for GTPase assays as well as for 31P NMR spectroscopy were first loaded with GTP by incubation in the presence of 20-fold excess GTP (Guanosine 5′-Triphosphate, Disodium Salt, CAT # 371701, Calbiochem) in 50 mM Tris HCl pH 7.5 ...
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bioRxiv - Plant Biology 2021Quote: ... Samples were incubated on ice for 5 min with gentle vortex and then filter through two layers of Miracloth (Calbiochem) and centrifuged at 4°C ...
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bioRxiv - Neuroscience 2022Quote: ... we gently separated the retina from sclera and RPE using fine forceps or electrically-sharpened tungsten electrodes (74) and immediately started incubation in papain solution (5 U/mL papain Calbiochem#5125 ...
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bioRxiv - Microbiology 2019Quote: ... or pronase (a mixture of endo- and exoproteases from Streptomyces griseus at a final concentration of 5, 10 or 50 μg/ml; Calbiochem, UK) for 30 min ...
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bioRxiv - Microbiology 2019Quote: ... media in the apical chamber were replaced with complete DMEM/F12 plus 5 μM of the γ-secretase inhibitor IX (DAPT; Calbiochem) while the basolateral media were replenished with the 1:1 stem cell/L-WRN media with 10μM Y-27632 (Calbiochem ...
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bioRxiv - Neuroscience 2019Quote: ... we incubated live DIV21 hippocampal cultures infected with control or UPF2 shRNAs at 37°C for 5 min with GluR1 antibody (Calbiochem, PC246) to allow labeling of surface GluR1 ...
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bioRxiv - Microbiology 2022Quote: ... For fungal culture filtrates a 106 spores/ml culture of RPMI was incubated at 37 ºC and 5% CO2 for 48 hours and then filtered through five layers of Miracloth (Calbiochem, UK) and then a 0.45 μm syringe filter to remove any spores and hyphal fragments ...
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bioRxiv - Neuroscience 2020Quote: Mice were submitted to 24 h of fasting and then received ip injections with either saline (0.9% NaCl) or mouse recombinant leptin (5 mg/kg body weight, Calbiochem, Billerica, MA, USA) immediately before the dark cycle ...
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bioRxiv - Immunology 2021Quote: ... The cells were then treated with SplB (5 μg/mL) or increasing concentrations of human plasma thrombin (PAR1 and PAR4 agonist, Calbiochem-EMD Millipore) or porcine trypsin (PAR2 agonist ...
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bioRxiv - Neuroscience 2020Quote: Mice were submitted to 12 h of fasting and then received ip injections with mouse leptin (5 mg/kg body weight, Calbiochem, Billerica, MA, USA). After 1 h ...