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Citations for Calbiochem :
551 - 600 of 604 citations for 6 Bromo 3 4 dihydro 2H 1 benzopyran 3 methanamine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... and 2 mM glutamine for 24 h prior to treatment with either TGF-β (1 ng/mL, Millipore Calbiochem) or 10 uM TGF-β inhibitors (SB431542 ...
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bioRxiv - Cell Biology 2019Quote: ... cells were lysed at 1 × 109 cells/ml in ice-cold lysis buffer (0.1 µM TLCK, 1 µg/ml Leupeptin, Phosphatase Inhibitor Cocktail II (Calbiochem),1 mM PMSF ...
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bioRxiv - Biochemistry 2019Quote: ... which were grown to an optical density of 0.6–0.8 at 600 nm and were induced with 1 mM isopropyl-β-D-thiogalactopyranoside (CALBIOCHEM) for 20 h at 18 °C ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were then harvested using lysis buffer (50mM HEPES pH7.4, 150mM NaCl, 1mM MgCl2, 1mM NaF, 1% Triton-x-100, protease inhibitor cocktail I [Calbiochem) and centrifuged at 14,000rpm for 10 minutes ...
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bioRxiv - Cancer Biology 2022Quote: ... collected and lysed in lysis buffer RGMT (50mM HEPES pH 7.4, 150mM NaCl, 1mM MgCl2, 1mM NaF, 1% Triton-x-100, plus protease inhibitor cocktail I [Calbiochem]). Cellular lysates were cleared by centrifugation and 225µL of total protein extract was incubated with biotin-tagged CIGB-300 (100μM ...
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bioRxiv - Molecular Biology 2022Quote: ... The cell pellet was lysed at 0.5 × 109 cells/ml in ice-cold lysis buffer (0.1 mM TLCK, 1 μg/ml Leupeptin, 1x Phosphatase Inhibitor Cocktail II tablet (Calbiochem), 1 mM PMSF ...
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bioRxiv - Developmental Biology 2022Quote: ... Tissues were homogenized in cold lysis buffer (0.1% NP-40, 50mM Tris-HCl [pH 7.2], 250mM NaCl, 2mM EDTA, phosphatase inhibitor mixture I and II [Calbiochem] and one tablet of Minicomplete [Roche] per 10ml ...
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bioRxiv - Cell Biology 2022Quote: ... alone bound to glutathione sepharose 4B beads in 0.7 ml of binding buffer (1X PBS, 150 mM NaCl, 1% Triton X-100, Calbiochem EDTA-free protease inhibitor cocktail set III ...
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bioRxiv - Immunology 2022Quote: ... and 1 × 106 cells were treated with rapamycin (10 ng/ml, 30 min, 37°C; Calbiochem, San Diego, CA) prior to treatment with rhGH.
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bioRxiv - Molecular Biology 2022Quote: ... pellets were resuspended in 800 μl of ice-cold wash buffer (50 mM NaH2PO4, 300 mM NaCl, 0.05% Tween, 1:200 diluted protease inhibitor cocktail set III (Calbiochem)) supplemented with 0.1 U/μL of recombinant RNase inhibitor (wash buffer-RIn ...
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bioRxiv - Biochemistry 2023Quote: ... Culture was then cooled to 16℃ and induced with 0.4 mM dioxane-free isopropyl B-D-1-thiogalactopyranoside (IPTG) (Calbiochem) overnight at 16℃ ...
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bioRxiv - Biochemistry 2023Quote: ... Culture was then cooled to 16℃ and induced with 0.5 mM dioxane-free isopropyl B-D-1-thiogalactopyranoside (IPTG) (Calbiochem) overnight at 16℃ ...
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bioRxiv - Biochemistry 2023Quote: ... Ubiquitin was expressed as described above except that the 2 L culture was induced with 0.4 mM dioxane-free isopropyl B-D-1-thiogalactopyranoside (IPTG) (Calbiochem) for four hrs at 37℃ ...
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bioRxiv - Biophysics 2023Quote: ... or FGFJI (20 ng ml−1, bFGF, R&D Systems; and 200 nM JAK inhibitor, Calbiochem, Merck Millipore, Germany). Cells were collected for mechanical characterization and RNA-Seq after 5-day exposure to the respective culture conditions (30).
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bioRxiv - Cancer Biology 2022Quote: ... Boston Bioproducts (Cat# BP-115X) supplemented with protease inhibitor cocktail at a dilution of 1: 100 (Calbiochem, Cat#539131) and phosphatase inhibitors at a dilution of 1 ...
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bioRxiv - Biochemistry 2023Quote: ... 0.2 mM DTT) supplemented with protease inhibitors (0.1 mM PMSF and 1:500 Cocktail IV (Calbiochem; San Diego, CA), and lysed by two passes through a French press at 1000 psi ...
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bioRxiv - Biochemistry 2023Quote: ... Culture was then cooled to 16℃ and induced with 0.4 mM dioxane-free isopropyl B-D-1-thiogalactopyranoside (IPTG) (Calbiochem) overnight at 16℃ ...
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bioRxiv - Neuroscience 2023Quote: ... Heads were snap-frozen in liquid nitrogen then homogenized using a mechanical homogenizer in 100 μL TBS (50mM Tris-HCl, pH 8.0) containing 1:200 protease inhibitor cocktail set III (Calbiochem). Homogenates were centrifuged at 16,000 g at 4°C for 30 min ...
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bioRxiv - Molecular Biology 2023Quote: Whole-cell protein extracts were prepared using hot lysis buffer (1%SDS, 5mM EDTA, 50mM Tris, pH7.5) supplemented with protease inhibitor mixture (Calbiochem). Protein samples were diluted and boiled in 5X protein loading buffer (10% SDS ...
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bioRxiv - Neuroscience 2024Quote: ... 1 mM Na3VO4 and 500 nM Cantharidin (#3322.1 Roth)] and commercial protease inhibitors (protease inhibitor cocktail III, #539134, Calbiochem). Phosphatase inhibitors were omitted when Lambda phosphatase treatment was required ...
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bioRxiv - Immunology 2021Quote: ... GMDCs were incubated for 2 h at 37°C with 50 µM MARK inhibitor (MARK/Par-1 Activity Inhibitor, 39621; Calbiochem), 50 nM SIK inhibitor (HG-9-91-01 ...
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bioRxiv - Molecular Biology 2019Quote: ... 0.1 mM PMSF and 0.1 μM Aprotinin) using a Dounce homogenizer (10 strokes, 400 rpm) and filtered through two layers of Mira cloth (Calbiochem). The filtrate was centrifuged at 600g at 4° C for 2 min to remove crud and unbroken cells ...
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bioRxiv - Cancer Biology 2022Quote: ... Whole cell samples were ground in liquid nitrogen and lysed in 8 M urea supplemented with 1% protease inhibitor cocktail (Calbiochem). Lysates were centrifuged at 12,000 x g and the supernatant was quantified by BCA assays (Beyotime) ...
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bioRxiv - Microbiology 2022Quote: ... The furin inhibitor decanoyl-RVKR-cmk and mannosidase-1 inhibitor deoxymannojirmycin (DMJ) were purchased from Calbiochem (San Diego, California, USA).
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bioRxiv - Cancer Biology 2020Quote: ... slides were counterstained with DAPI (1:50000 from 10 mg/ml stock solution, Serva Eletrophoresis) and embedded with geltol (Calbiochem). 2 representative pictures of 4 biological replicates per genotype were taken with LSM 5 Exciter (Zeiss ...
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bioRxiv - Cell Biology 2019Quote: ... Crosses were performed by random spore analysis (1, 2) followed by marker selection (Hygromycin/Kanamycin resistance, ura/leu auxotrophy, or sensitivity to 5 µM 1NM-PP1 (Calbiochem) for strains carrying the Ark1-as3 allele ...
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bioRxiv - Genetics 2020Quote: Drosophila larval brains were homogenized with pestles in RIPA buffer plus the addition of proteinase inhibitors 1:200 (539134, Calbiochem). The homogenate was centrifuged at 13000 rpm for 20 minutes at 4°C ...
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bioRxiv - Developmental Biology 2020Quote: Mammalian cells were lysed using RIPA buffer (50mM Tris, pH 7.4, 150mM NaCl, 1%NP-40, 0.5%sodium deoxycholate) with protease inhibitors (Calbiochem, Billerica, MA). Protein samples were separated on 4–15%gradient TGX precast gels (Bio-Rad ...
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bioRxiv - Cell Biology 2021Quote: ... the cell pellet was lysed by sonication in lysis buffer (2mM Tris–HCl pH 7.4, 0.5M NaCl, 20mM imidazole, 10% glycerol, Protease Inhibitor Cocktail Set III Calbiochem, 1:10,000) and centrifuged (10,000g ...
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bioRxiv - Neuroscience 2022Quote: Supernatant was removed and beads were resuspended in 250μl of antibody buffer (wash buffer + 0.05% Digitonin (Calbiochem #11024-24-1) + 2mM EDTA ...
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bioRxiv - Biochemistry 2022Quote: ... lysed in M-PER mammalian protein extraction reagent (Thermo-Fisher) with 1/1000 of the EDTA-free protease inhibitor cocktail Set III (Calbiochem), and centrifuged for 30 min at 15,000 × g at 4°C ...
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bioRxiv - Immunology 2022Quote: ... Cells were left unstimulated or stimulated for indicated time at 37 °C with PMA (10 ng/ml; Alexis) and ionomycin (1 μM; Calbiochem) or solvent (DMSO) ...
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bioRxiv - Neuroscience 2023Quote: ... mg of tissue/µl of NP-40 lysis buffer (20mM Tris- HCl pH 7.4, 150 mM NaCl, 100mM EDTA pH 7.4, 1% NP-40; Calbiochem Cat#492016) with protease inhibitors (cOmplete™ ...
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bioRxiv - Cell Biology 2023Quote: ... and cells were held for 16 hours in 1% Lipoprotein Deficient Serum (LDS) (Biomedical Technologies, BT907) and 25 μg/ml ALLN (Calbiochem) for 30 min prior to harvesting.
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bioRxiv - Cell Biology 2021Quote: Commercially available antibodies against the following proteins were used for Western blotting in the indicated dilutions: BRCA2 (OP95, Calbiochem, 1:1000), RAD51 (70-001 ...
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bioRxiv - Neuroscience 2022Quote: ... the slices were incubated overnight in a mixture of primary antibodies anti-FOS (rabbit anti-FOS, 1:10000, cat: p38, Calbiochem, USA) and anti-GAD67 (mouse anti-GAD67 ...
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bioRxiv - Bioengineering 2022Quote: ... All the above stages were performed in cold phosphate-buffered saline (PBS) with 1 × Protease Inhibitor cocktail set I (Calbiochem 539,131). Eyes that were initially fixed in 10% NBF or 2.5% PFA were dissected to create eyecups ...
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bioRxiv - Molecular Biology 2023Quote: ... cultures were lysed with a solution containing SYBR green (1:5000, Invitrogen Cat. No. S7567) (Lysis buffer: 0.16% saponin (Calbiochem/EMD #558255), 0.05 M TRIS-HCl (Roche Cat ...
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bioRxiv - Genetics 2023Quote: ... Raw values were normalized by geometric mean and used for subsequent housekeeping normalization (α-tubulin values; 1:10,000 mouse anti-tubulin; Calbiochem CP06) from the same blot.
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bioRxiv - Physiology 2023Quote: ... MLEC were incubated with appropriate fluorophore-labelled secondary antibodies for 1 hour and mounted with Mowiol (Calbiochem, San Diego, CA, USA). Nuclei were visualized by DAPI staining ...
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bioRxiv - Molecular Biology 2023Quote: ... 0.1 mM EGTA, 1% Triton X-100, 0.1% sodium deoxycholate, 0.5% SLS, 0.1% SDS, 0.5 mM PMSF, Calbiochem Complete Protease Inhibitor Cocktail V). The suspension was incubated for 30 min at 4°C and then sonicated with Covaris ME220 focused-ultrasonicator (50 alternating 15-s ON and 45-s OFF intervals ...
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bioRxiv - Neuroscience 2019Quote: Pre-sorted synaptosomes (~750 μl) on iced were mixed with ice cold PBS containing protease inhibitor cocktail EGTA-free (Calbiochem, 1:1000) and RNasin (Promega ...
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bioRxiv - Bioengineering 2020Quote: ... Tested additives were added to the qRT-PCR reaction mix at the following concentrations: 1% w/v Tween-20 (Calbiochem 655204-100mL), 0.8 µg/µL BSA (Fisher BP675-1) ...
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Myosin VI regulates ciliogenesis by promoting the turnover of the centrosomal/satellite protein OFD1bioRxiv - Cell Biology 2021Quote: ... pH 7.5, 250 mM NaCl, 1 mM EDTA, 5% Glycerol, 0.1% NP40, Protease Inhibitor Cocktail set III, Calbiochem, and 0.1 mM PMSF). Sonicated lysates were cleared by centrifugation at 16,000 rpm for 30 minutes ...
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bioRxiv - Physiology 2023Quote: ... Normal goat serum was used as a blocking agent and slides were incubated in biotinylated hyaluronan binding protein (HABP, 1:500, CalBiochem, Burlington, MA) for one hour ...
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bioRxiv - Neuroscience 2021Quote: ... cells were rinsed two times with pre-warmed medium and incubated for 30 min at 37°C with rabbit anti-GluA1 (1/150; Calbiochem/Millipore, RRID: AB_564636) in culture medium ...
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bioRxiv - Cell Biology 2021Quote: ... NaCl-HEPES buffer with antibodies but without uEV sample as well as stained sample supplemented with 1% NP40 (Calbiochem, San Diego, CA, USA) were analyzed as controls ...
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bioRxiv - Cancer Biology 2023Quote: ... hatched zebrafish embryos were engrafted via the Duct of Cuvier with 1×105 cells/µL tumor cells resuspended in 2% polyvinypyrrolidone 40 (PVP-40, Calbiochem, San Diego, USA) using borosilicate glass capillary needles (1mm O.D ...
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bioRxiv - Cell Biology 2023Quote: ... RPE-1 cells transfected with either siR-NAs targeting Luciferase or MiniBAR for 72 h were treated with Y27632 (Calbiochem 146986-50-7) at 50 µM for 24 h in DMEM-F12 without SVF before fixation.
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bioRxiv - Molecular Biology 2023Quote: ... the mice were anaesthetized using 2% isoflurane and given a retro-orbital injection of 21.75 mg kg−1 body weight puromycin (Calbiochem, San Diego, CA, USA) in saline to measure muscle protein synthesis73.