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Citations for Calbiochem :
101 - 150 of 153 citations for 6 Amino 5 formamido uracil since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... 5 μg/ml RNAse A pH 7.4 and protease inhibitor cocktail set III EDTA-free (Calbiochem) added of 1 mM PMSF ...
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bioRxiv - Plant Biology 2021Quote: ... and the supernatant re-centrifuged for 5 min and filtered through a layer of Miracloth (Calbiochem). Clarified extracts were incubated with pre-washed GFP-Trap®_M magnetic beads on an end-to-end tube rotator at 4°C for 1 hr ...
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bioRxiv - Plant Biology 2021Quote: ... 5-day-old seedlings were transferred to medium supplemented with 0.6 mg/L bleomycin sulphate (Calbiochem) for 24 h.
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bioRxiv - Plant Biology 2019Quote: ... 35 μL water and 5 μL 100 mM monobromobimane in acetonitrile (Thiolyte® MB, Calbiochem, www.merckmillipore.com) were mixed and added to the samples ...
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bioRxiv - Immunology 2019Quote: ... cultured in R10 for several days and activated with 5 μg/ml phytohaemagglutinin (PHA-L, Calbiochem, Millipore Sigma ...
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bioRxiv - Cell Biology 2023Quote: ... in PBS for 5 min at RT and cells were mounted in Mowiol 4-88 (Calbiochem) solution (Wei and Seemann ...
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bioRxiv - Cancer Biology 2022Quote: ... incubated with 30 nM DAPI for 5 minutes and mounted on slides with FluorSave reagent (Calbiochem 345789). Immunofluorescence images were taken using a Keyence BZ-X800E Fluorescent Microscope with a 40X or 60X objective lens ...
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bioRxiv - Biochemistry 2021Quote: ... whereupon the final two wash steps were replaced with a single wash in 1.0 ml of 5 mM sodium phosphate buffer (pH 7.4) containing 0.1% (w/v) sarkosyl (Calbiochem) and 0.3% (w/v ...
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bioRxiv - Biochemistry 2021Quote: Uridine-5-triphosphate (UTP; cat #6701) and phorbol 12,13-dibutyrate (PDBu; cat #524390) were purchased from Calbiochem. Calyculin A (cat #9902 ...
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bioRxiv - Neuroscience 2023Quote: Cortical neurons were incubated with 4 μM fura-2 acetoxymethyl ester (AM, Calbiochem, CAS 108964-32-5) 40 min at 37°C ...
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bioRxiv - Microbiology 2021Quote: ... The conidiospores were washed by 5 mL distilled water and filtrated through two layers of Miracloth (Calbiochem, Germany). The yield of spores was measured by a hemocytometer ...
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bioRxiv - Biophysics 2022Quote: ... were mixed in 70 μL of assembly buffer (50 mM HEPES-KOH, pH 7.5, 300 mM KCl, 5 mM MgCl2, 2 mM TCEP, 4% glycerol, 2 mM ATPγS [Calbiochem]) for 5 min at 25 °C ...
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bioRxiv - Cancer Biology 2021Quote: Cell pellets were sonicated in ice-cold lysis buffer (containing 20 mM Tris-HCl, pH 7.4, 5 mM EDTA, 10 mM Na4P2O7 (Calbiochem), 100 mM NaF ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... and the phenylacetamide compound (S)-2-(4-chlorophenyl)-3,3-dimethyl-N-(5-phenylthiazol-2-yl)butanamide (PA;Cmp58 (Wang et al., 2010)) was obtained from Calbiochem-Merck Millipore (Billerica ...
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bioRxiv - Neuroscience 2019Quote: ... Membranes were blocked to reduce nonspecific binding using 5% milk with PBS-TWEEN® tablets (PBS-T) (Calbiochem, USA) at room temperature (RT ...
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bioRxiv - Immunology 2020Quote: ... Fixed cells were washed three times in PBS and permeabilized by incubating them in PBS containing 5% non-fat milk and 0.05% saponin (Calbiochem). Coverslips were placed in primary antibody for 1 hour then washed 3x in PBS and placed in secondary antibody ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... and the phenylacetamide compound (S)-2-(4-chlorophenyl)-3,3-dimethyl-N-(5-phenylthiazol-2-yl)butanamide (Cmp58) was obtained from Calbiochem-Merck Millipore (Billerica ...
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bioRxiv - Cell Biology 2020Quote: ... and the phenylacetamide compound (S)-2-(4-chlorophenyl)-3,3-dimethyl-N-(5-phenylthiazol-2-yl) butanamide (Cmp58) was obtained from Calbiochem-Merck Millipore (Billerica ...
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bioRxiv - Cell Biology 2020Quote: ... The allosteric FFAR2 modulator Cmp58 ((S)-2-(4-chlorophenyl)-3,3-dimethyl-N-(5-phenylthiazol-2-yl)butanamide was obtained from Calbiochem-Merck Millipore (Billerica ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... and the phenylacetamide compound (S)-2-(4-chlorophenyl)-3,3-dimethyl-N-(5-phenylthiazol-2-yl)butanamide (PA;Cmp58 [30]) was obtained from Calbiochem-Merck Millipore (Billerica ...
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bioRxiv - Cell Biology 2023Quote: ... The homogenate was then incubated on ice for 5 minutes and filtered through 2 layers of Miracloth tissue (Calbiochem). The filtered homogenate was then centrifuged for 20 min at 480 rpm at 4°C to pellet any debris ...
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bioRxiv - Neuroscience 2023Quote: ... and counterstained with DAPI (10 min) before being washed (3x 5-min PBS 0.1M) and mounted on gelatin slides and coated with FluorSave (Calbiochem). Images of the whole section were taken using Axioscan.Z1 (Zeiss ...
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bioRxiv - Molecular Biology 2023Quote: ... 10 mM EDTA, 1% NP-40, 1% sodium deoxycholate, 0.1% SDS, 1 mM DTT, 5 mM PMSF, and 1:100 Calbiochem Complete Protease Inhibitor Cocktails VII and V ...
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bioRxiv - Cell Biology 2020Quote: ... They were then pelleted down by centrifuging at 1000 rpm for 5 min and then washed with PBS supplemented with 10 μM MG-132 (Calbiochem). After centrifugation ...
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bioRxiv - Molecular Biology 2019Quote: ... 0.1 mM PMSF and 0.1 μM Aprotinin) using a Dounce homogenizer (5 strokes, 300 rpm) and filtered through two layers of Mira cloth (Catalog# 475855, Calbiochem). The filtrate was centrifuged at 200g at 4° C for 2 min to remove crud and unbroken cells ...
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bioRxiv - Plant Biology 2019Quote: ... 35 μL water were added and the GSH was derivatised using 5 μL of 0.1 M monobromobimane (Thiolyte® MB, Calbiochem) in darkness for 15 min at 37°C ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were washed twice with 3 ml PBS at 400 g for 5 minutes at 4°C and 500 uL PI staining buffer was added (1X PBS, 50 μg/mL PI (Calbiochem), 2μg/mL RNase A (Sigma ...
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bioRxiv - Biochemistry 2020Quote: ... 1 µmol L-1 pepstatin A and 5 µmol L-1 phenyl-methane-sulfonyl-fluoride) was from Calbiochem (Darmstadt, Germany). Ammonium sulfate-depleted PK ...
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bioRxiv - Microbiology 2021Quote: ... confluent epithelial cell monolayers were treated with the following inhibitors at concentrations previously determined (5): SP600125 (Primary target: JNK1, JNK2, JNK3; 10 μM, Calbiochem), SB203580 (Primary target ...
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bioRxiv - Cancer Biology 2020Quote: ... After saturation with TBS Tween 5% BSA for 1h and hybridization of the membranes with primary overnight (BCR-ABL Ab3 Calbiochem, pCrkL Tyr207 Cell Signaling ...
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bioRxiv - Cell Biology 2022Quote: ... and AR-C118925 {5-[[5-(2,8-dimethyl-5H-dibenzo[a,d]cyclohepten-5-yl)-3,4-dihydro-2-oxo-4-thioxo-1(2H)-pyrimidinyl]methyl]-N-2H-tetrazol-5-yl-2-furancarboxamide} were obtained from Calbiochem-Merck Millipore (Billerica ...
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bioRxiv - Systems Biology 2022Quote: ... respectively) were resuspended in 1 mL of ChIP buffer containing 5 μl of protease inhibitor cocktail (CalbioChem, cat # 539134-1) (50 mM Tris-HCl pH 8 ...
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bioRxiv - Immunology 2022Quote: Enriched mouse NK cells from Dd−/− and Dd+/+ mice were incubated with SHP-1 inhibitor (denoted in the paper as iSHP-1) (NSC-87877, CAS 56932-43-5 – Calbiochem) of different concentrations or PBS of the amount corresponding to the highest iSHP-1 concentration ...
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bioRxiv - Neuroscience 2020Quote: ... 4×106 cells were transferred to one well of a 6-well plate and kept in suspension under rotation (95 rpm) for 24 hours with 5 μM of ROCK inhibitor (Y-27632; Calbiochem). Forty-eight hours later ...
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bioRxiv - Cell Biology 2022Quote: DLD1-Nup153AID cells were plated at 140K per well on glass coverslips and cultured for 8 hours in the absence or presence of 5 mM thymidine (Calbiochem). 1 mM Indole-3-acetic acid (IAA ...
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bioRxiv - Cell Biology 2019Quote: ... Crosses were performed by random spore analysis (1, 2) followed by marker selection (Hygromycin/Kanamycin resistance, ura/leu auxotrophy, or sensitivity to 5 µM 1NM-PP1 (Calbiochem) for strains carrying the Ark1-as3 allele ...
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bioRxiv - Systems Biology 2021Quote: Gsp1 variants for GTPase assays as well as for 31P NMR spectroscopy were first loaded with GTP by incubation in the presence of 20-fold excess GTP (Guanosine 5′-Triphosphate, Disodium Salt, CAT # 371701, Calbiochem) in 50 mM Tris HCl pH 7.5 ...
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bioRxiv - Plant Biology 2021Quote: ... Samples were incubated on ice for 5 min with gentle vortex and then filter through two layers of Miracloth (Calbiochem) and centrifuged at 4°C ...
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bioRxiv - Neuroscience 2022Quote: ... we gently separated the retina from sclera and RPE using fine forceps or electrically-sharpened tungsten electrodes (74) and immediately started incubation in papain solution (5 U/mL papain Calbiochem#5125 ...
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bioRxiv - Cell Biology 2022Quote: ... the P2Y2R antagonist AR-C118925 {5-[[5-(2,8-dimethyl-5Hdibenzo[α,d]cyclohepten-5-yl)-3,4-dihydro-2-oxo-4-thioxo-1(2H)-pyrimidinyl]methyl]-A-2H-tetrazol-5-yl-2-furan-carboxamide},and the Ca2+ probes thapsigargin and ionomycin were obtained from Calbiochem-Merck Millipore (Billerica ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were washed 3 times with PBS and fixed to a slide using 5 μL of Mowiol (4-88 Reagent, Calbiochem). Fluorescent images were acquired with a Leica Dmi8 microscope (Leica Microsystems).
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bioRxiv - Pathology 2024Quote: ... NETs formation was induced for 3 hours at 37°C and 5% CO2 by stimulation with 10 µM of PAF (Calbiochem) on primed neutrophils and 25 µM on non-primed ones ...
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bioRxiv - Microbiology 2019Quote: ... or pronase (a mixture of endo- and exoproteases from Streptomyces griseus at a final concentration of 5, 10 or 50 μg/ml; Calbiochem, UK) for 30 min ...
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bioRxiv - Microbiology 2019Quote: ... media in the apical chamber were replaced with complete DMEM/F12 plus 5 μM of the γ-secretase inhibitor IX (DAPT; Calbiochem) while the basolateral media were replenished with the 1:1 stem cell/L-WRN media with 10μM Y-27632 (Calbiochem ...
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bioRxiv - Neuroscience 2019Quote: ... we incubated live DIV21 hippocampal cultures infected with control or UPF2 shRNAs at 37°C for 5 min with GluR1 antibody (Calbiochem, PC246) to allow labeling of surface GluR1 ...
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bioRxiv - Cell Biology 2019Quote: ... (N-{(2S,4R)-4-(Biphenyl-2-ylmethyl-isobutyl-amino)-1-[2-(2,4-difluorobenzoyl)-benzoyl]-pyrrolidin-2-ylmethyl}8-3-(4-(2,4-dioxothiazoldin-5-ylidenemethyl)-phenyl]acrylamide (cPLA2 inhibitor, cPLA2 Inh, Calbiochem, EMD Biosciences), was used concomitantly with priming at 5 μM and MK-886 (FLAP Inh ...
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bioRxiv - Microbiology 2022Quote: ... For fungal culture filtrates a 106 spores/ml culture of RPMI was incubated at 37 ºC and 5% CO2 for 48 hours and then filtered through five layers of Miracloth (Calbiochem, UK) and then a 0.45 μm syringe filter to remove any spores and hyphal fragments ...
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bioRxiv - Neuroscience 2020Quote: Mice were submitted to 24 h of fasting and then received ip injections with either saline (0.9% NaCl) or mouse recombinant leptin (5 mg/kg body weight, Calbiochem, Billerica, MA, USA) immediately before the dark cycle ...
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bioRxiv - Immunology 2021Quote: ... The cells were then treated with SplB (5 μg/mL) or increasing concentrations of human plasma thrombin (PAR1 and PAR4 agonist, Calbiochem-EMD Millipore) or porcine trypsin (PAR2 agonist ...
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Myosin VI regulates ciliogenesis by promoting the turnover of the centrosomal/satellite protein OFD1bioRxiv - Cell Biology 2021Quote: ... pH 7.5, 250 mM NaCl, 1 mM EDTA, 5% Glycerol, 0.1% NP40, Protease Inhibitor Cocktail set III, Calbiochem, and 0.1 mM PMSF). Sonicated lysates were cleared by centrifugation at 16,000 rpm for 30 minutes ...