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Citations for Calbiochem :
301 - 350 of 353 citations for 5 Pyrimidinecarbonitrile 4 6 diamino 2 methoxy 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... After saturation with TBS Tween 5% BSA for 1h and hybridization of the membranes with primary overnight (BCR-ABL Ab3 Calbiochem, pCrkL Tyr207 Cell Signaling ...
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bioRxiv - Systems Biology 2022Quote: ... respectively) were resuspended in 1 mL of ChIP buffer containing 5 μl of protease inhibitor cocktail (CalbioChem, cat # 539134-1) (50 mM Tris-HCl pH 8 ...
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bioRxiv - Immunology 2022Quote: Enriched mouse NK cells from Dd−/− and Dd+/+ mice were incubated with SHP-1 inhibitor (denoted in the paper as iSHP-1) (NSC-87877, CAS 56932-43-5 – Calbiochem) of different concentrations or PBS of the amount corresponding to the highest iSHP-1 concentration ...
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bioRxiv - Neuroscience 2020Quote: ... 4×106 cells were transferred to one well of a 6-well plate and kept in suspension under rotation (95 rpm) for 24 hours with 5 μM of ROCK inhibitor (Y-27632; Calbiochem). Forty-eight hours later ...
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bioRxiv - Cell Biology 2022Quote: DLD1-Nup153AID cells were plated at 140K per well on glass coverslips and cultured for 8 hours in the absence or presence of 5 mM thymidine (Calbiochem). 1 mM Indole-3-acetic acid (IAA ...
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bioRxiv - Systems Biology 2021Quote: Gsp1 variants for GTPase assays as well as for 31P NMR spectroscopy were first loaded with GTP by incubation in the presence of 20-fold excess GTP (Guanosine 5′-Triphosphate, Disodium Salt, CAT # 371701, Calbiochem) in 50 mM Tris HCl pH 7.5 ...
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bioRxiv - Plant Biology 2021Quote: ... Samples were incubated on ice for 5 min with gentle vortex and then filter through two layers of Miracloth (Calbiochem) and centrifuged at 4°C ...
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bioRxiv - Neuroscience 2022Quote: ... we gently separated the retina from sclera and RPE using fine forceps or electrically-sharpened tungsten electrodes (74) and immediately started incubation in papain solution (5 U/mL papain Calbiochem#5125 ...
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bioRxiv - Pathology 2024Quote: ... NETs formation was induced for 3 hours at 37°C and 5% CO2 by stimulation with 10 µM of PAF (Calbiochem) on primed neutrophils and 25 µM on non-primed ones ...
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bioRxiv - Molecular Biology 2020Quote: ... 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reagent was obtained from Calbiochem (San Diego, CA, USA).
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bioRxiv - Physiology 2021Quote: ... and phosphatase inhibitors (2 μM microcystin-LR, and 1× Phosphatase-Inhibitor Sets I and II (Calbiochem, Hayward, CA). We centrifuged homogenates at 4,000 g for 15 minutes to remove debris ...
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bioRxiv - Pathology 2023Quote: ... 2% DDM) supplemented with 1:1000 (v/v) Protease Inhibitor Cocktail Set III (Calbiochem, La Jolla, CA, USA). Rat anti-HA (3F10 ...
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bioRxiv - Cell Biology 2023Quote: ... mouse organs were homogenized in 2% SDS lysis buffer containing protease inhibitor mixture III (# 535140, Calbiochem, Billerica, MA) and phosphatase inhibitors (#P2850 ...
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bioRxiv - Cell Biology 2020Quote: Proteasome activity was quantitatively assessed in H4 cell extracts using the β5-selective fluorogenic substrate succinyl-leucine-leucine-valine-tyrosine-4-methyl-7-courmarylamide (Suc-LLVY-AMC, Calbiochem, Burlington, MA, USA) using an adapted protocol 87 ...
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bioRxiv - Microbiology 2019Quote: ... or pronase (a mixture of endo- and exoproteases from Streptomyces griseus at a final concentration of 5, 10 or 50 μg/ml; Calbiochem, UK) for 30 min ...
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bioRxiv - Microbiology 2019Quote: ... media in the apical chamber were replaced with complete DMEM/F12 plus 5 μM of the γ-secretase inhibitor IX (DAPT; Calbiochem) while the basolateral media were replenished with the 1:1 stem cell/L-WRN media with 10μM Y-27632 (Calbiochem ...
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bioRxiv - Neuroscience 2019Quote: ... we incubated live DIV21 hippocampal cultures infected with control or UPF2 shRNAs at 37°C for 5 min with GluR1 antibody (Calbiochem, PC246) to allow labeling of surface GluR1 ...
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bioRxiv - Microbiology 2022Quote: ... For fungal culture filtrates a 106 spores/ml culture of RPMI was incubated at 37 ºC and 5% CO2 for 48 hours and then filtered through five layers of Miracloth (Calbiochem, UK) and then a 0.45 μm syringe filter to remove any spores and hyphal fragments ...
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bioRxiv - Cancer Biology 2021Quote: ... and 2 mM glutamine for 24 h prior to treatment with either TGF-β (1 ng/mL, Millipore Calbiochem) or 10 uM TGF-β inhibitors (SB431542 ...
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Proteomics of broad deubiquitylase inhibition unmasks redundant enzyme function to reveal substratesbioRxiv - Biochemistry 2020Quote: Interphase extract was prepared as previously described(Murray AW 1991) but using 2 μg/ml calcium ionophore (A23187, Calbiochem) for egg activation ...
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bioRxiv - Molecular Biology 2022Quote: ... Chronic low-dose inflammation was mimicked in 3T3-L1 adipocytes by incubation with 2 ng/mL TNFα (TNF; Calbiochem) for 4 d ...
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bioRxiv - Plant Biology 2022Quote: ... except that the resuspension of protein precipitate was in modified denaturing urea buffer (8 M urea, 2% SDS, 100 mM HEPES-KOH pH 7.4, 10 mM EDTA and protease inhibitor [Calbiochem, now Merck ...
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bioRxiv - Physiology 2023Quote: ... Fura-2 AM and BAPTA-AM (Cat# F1221, Molecular Probes, Eugene, OR, USA and CAS# 126150-97-8, Calbiochem San Diego ...
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bioRxiv - Biochemistry 2023Quote: ... Ubiquitin was expressed as described above except that the 2 L culture was induced with 0.4 mM dioxane-free isopropyl B-D-1-thiogalactopyranoside (IPTG) (Calbiochem) for four hrs at 37℃ ...
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bioRxiv - Cell Biology 2023Quote: The antibodies listed in Tables 1 and 2 were diluted as indicated in PBS containing 1% BSA (126575, Calbiochem).
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bioRxiv - Cancer Biology 2023Quote: NMDI-14 inhibitor (Ethyl 2-(((6,7-dimethyl-3-oxo-1,2,3,4-tetrahydro-2-quinoxalinyl)acetyl)amino)-4,5-dimethyl-3-thiophenecarboxylate) was purchased from Calbiochem (Cat. Nr. 530838) and reconstituted in DMSO (10mg/ml ...
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bioRxiv - Cell Biology 2023Quote: ... The antibodies (Tables 2 and 3) were diluted according to the tables in PBS containing 1% BSA (126575, Calbiochem).
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bioRxiv - Neuroscience 2020Quote: ... At 4 hours post fertilisation (hpf) embryos were treated with 100µM DAPT (In solution™ γ-secretase inhibitor IX, Calbiochem, San Diego, CA, USA) in 1% DMSO in E3 medium ...
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bioRxiv - Neuroscience 2020Quote: Mice were submitted to 24 h of fasting and then received ip injections with either saline (0.9% NaCl) or mouse recombinant leptin (5 mg/kg body weight, Calbiochem, Billerica, MA, USA) immediately before the dark cycle ...
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bioRxiv - Immunology 2021Quote: ... The cells were then treated with SplB (5 μg/mL) or increasing concentrations of human plasma thrombin (PAR1 and PAR4 agonist, Calbiochem-EMD Millipore) or porcine trypsin (PAR2 agonist ...
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Myosin VI regulates ciliogenesis by promoting the turnover of the centrosomal/satellite protein OFD1bioRxiv - Cell Biology 2021Quote: ... pH 7.5, 250 mM NaCl, 1 mM EDTA, 5% Glycerol, 0.1% NP40, Protease Inhibitor Cocktail set III, Calbiochem, and 0.1 mM PMSF). Sonicated lysates were cleared by centrifugation at 16,000 rpm for 30 minutes ...
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bioRxiv - Immunology 2021Quote: ... GMDCs were incubated for 2 h at 37°C with 50 µM MARK inhibitor (MARK/Par-1 Activity Inhibitor, 39621; Calbiochem), 50 nM SIK inhibitor (HG-9-91-01 ...
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bioRxiv - Immunology 2019Quote: ... Injection inoculum was prepared from an overnight liquid culture in the log-phase of growth resuspended in 2% polyvinylpyrrolidone40 (PVP40) solution (CalBiochem) as previously described (40) ...
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bioRxiv - Cell Biology 2021Quote: ... The antibodies listed in Table 2 and 3 were diluted according to the tables in PBS containing 1% BSA (126575, Calbiochem).
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bioRxiv - Cell Biology 2021Quote: ... Cells were arrested in S phase or at the G1/S boundary by single or double thymidine (2 mM, Calbiochem) treatment ...
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bioRxiv - Developmental Biology 2022Quote: ... Drosophila ovaries from aPKCas4 flies (prepared as previously described in the optogenetic experiments section) were cultured ex vivo for 2 hours in the presence of the ATP analog 1NA-PP1 (Calbiochem; prepared in DMSO ...
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bioRxiv - Physiology 2021Quote: ... or 0.2 mL (for lung) HEPES-buffered saline plus Phosphatase Inhibitor Cocktail Set II and Protease Inhibitor Cocktail Set III (Calbiochem). Protein was measured using a Pierce™ BCA protein assay kit (Thermo Fisher ...
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bioRxiv - Molecular Biology 2021Quote: ... Fresh 2-day conidia from each strain were harvested from ANM plates in PBS solution and filtered through a Miracloth (Calbiochem). For each strain ...
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bioRxiv - Cell Biology 2023Quote: ... Equivalent amounts of protein (1-2 mg) were incubated with 1 μg indicated antibody and 20 μL protein A/G beads (#IP05, Calbiochem) at 4 ℃ overnight ...
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bioRxiv - Cell Biology 2021Quote: 1.5 µM ethylene glycol-bis(β-aminoethyl ether)-N,N,N’,N’-tetraacetic acid (EGTA) or 5 µM cyclopiazonic acid (CPA, CalBiochem-EMD Millipore). For Ca2+ replenishment ...
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bioRxiv - Neuroscience 2020Quote: Mice were submitted to 12 h of fasting and then received ip injections with mouse leptin (5 mg/kg body weight, Calbiochem, Billerica, MA, USA). After 1 h ...
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bioRxiv - Microbiology 2021Quote: ... to OD600 1 were incubated with 750 μl of lysis buffer (100 mM EDTA [pH 8.0], 1 mM MgCl2, 5 μg/ml lysostaphin, 10 μg/ml DNase, proteinase inhibitor [cocktail set III from Calbiochem] diluted 1:100 in PBS) for 30 min at 37°C and homogenized three times with 500 μl zirconia-silica beads (0.1 mm diameter from Carl-Roth ...
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bioRxiv - Cancer Biology 2020Quote: ... plate wells were washed with PBS containing 2% FBS and incubated overnight with biotinylated HA-binding protein (3 μg/ml, Calbiochem-EMD Millipore) at 4° C (34) ...
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bioRxiv - Cancer Biology 2020Quote: ... plate wells were washed with PBS containing 2% FBS and incubated overnight with biotinylated HA-binding protein (3 μg/ml, Calbiochem-EMD Millipore) at 4° C (16) ...
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bioRxiv - Cell Biology 2020Quote: ... and all cell types were lysed with Pierce™ RIPA Buffer (ThermoScientific 89900) supplemented with 1X Phosphatase Inhibitor Cocktail Set 2 (Calbiochem 5246251) and 1X Protease Inhibitor Cocktail Set 1 (Calbiochem 539131) ...
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bioRxiv - Cell Biology 2021Quote: ... plate wells were washed with PBS containing 2% FBS and incubated overnight with biotinylated HA-binding protein (3 μg/ml, Calbiochem-EMD Millipore) at 4° C (7) ...
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bioRxiv - Biochemistry 2020Quote: ... 5-8×106 cell pellets were washed twice and resuspended in cold DPBS and incubated on ice for 10 min with 2 mg/mL digitonin (Calbiochem, #3000410-5GM). Next ...
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bioRxiv - Biochemistry 2021Quote: ... PTP1B KO Ramos were transfected with a doxycycline inducible tet-on vector and exposed to 2 μg/ml doxycycline (Calbiochem, Merck, Darmstadt, Germany) 24 to 48 h before the experiment ...
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bioRxiv - Cancer Biology 2020Quote: Dissected CP specimens were dissociated using forceps under a stereoscope followed by enzymatic digestion at 37°C for 20 minutes with pronase (2 mg/ml, 537088, Calbiochem, San Diego, CA) in Hank’s balanced salt solution (HBSS ...
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bioRxiv - Molecular Biology 2023Quote: ... the cells were solubilized for 1 h at 4□°C in buffer (50□mM HEPES-NaOH [pH 7.5], 300□mM NaCl, 2% (w/v) N-dodecyl β-d-maltoside (DDM, Calbiochem, San Diego, CA), protease inhibitor cocktail ...