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Citations for Calbiochem :
401 - 450 of 546 citations for 8 1 Piperazinyl imidazo 1 2 a pyrazine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... PamChip arrays (PamGene #88101) were blocked for 20 cycles with 1% Bovine serum albumin (Calbiochem #126609) in Tris-buffered saline (TBS) ...
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bioRxiv - Neuroscience 2023Quote: ... protease inhibitors (PI, 1:4000 dilution of protease inhibitor cocktail 3 w/o EDTA, Calbiochem, #539134) and benzonase (1:1000 ...
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bioRxiv - Developmental Biology 2023Quote: Cells were harvested in RIPA buffer with 1:100 protease inhibitor cocktail set I (Calbiochem, #539131) and phosphatase inhibitors (1:100 Na2Ov4 ...
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bioRxiv - Cell Biology 2024Quote: ... Membranes were incubated overnight at 4°C,with mouse anti-PGC-1α (Calbiochem, KP9803, 1:1000), mouse anti-PINK1 (Santa Cruz Biotechnology ...
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bioRxiv - Cell Biology 2019Quote: ... cells were resuspended in KHM buffer containing CaCl2 (1 mM) and treated with Stapylocossus aureus nuclease (Calbiochem) (150 U/ml ...
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bioRxiv - Molecular Biology 2019Quote: ... ATP regeneration system (1 mM ATP, 25 mM creatine monophosphate [Sigma], 0.03 U/µL creatine kinase [Calbiochem] ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The master mix recipe contained polyclonal anti-rabbit anti-MLH1 (Calbiochem, San Diego CA; diluted 1:50) or anti-rabbit anti-DMC1 (mix of DMC1) ...
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bioRxiv - Cell Biology 2022Quote: ... resuspended in 6 ml lysis buffer (1X PBS, 500 mM NaCl, 1% Triton X-100, lysozyme, Calbiochem EDTA-free protease inhibitor cocktail set III ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were washed with ice cold PBS and lysed in IP lysis buffer (50 mM Tris, pH 7.5, 10 mM NaCl, 2.5 mM MgCl2, 1 mM DTT, 0.01% digitonin [Calbiochem 300410] and protease inhibitors ...
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bioRxiv - Cell Biology 2023Quote: ... Controls were treated for 30 minutes with KRBH + 2.8 mM glucose + 1 μM latrunculin B (Calbiochem, #428020) or 1 μM jasplakinolide (Millipore Sigma ...
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bioRxiv - Cell Biology 2023Quote: ... GFP-Rab11 WTlow and DNlow cell culture media was also supplemented with 1.25 μg.mL-1 puromycin (Calbiochem). Cells were regularly tested for mycoplasma contamination with the LookOut mycoplasma PCR detection kit (Sigma ...
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bioRxiv - Microbiology 2023Quote: ... cells were pretreated 1 h at 37°C with 20 nM bafilomycin A1 (Calbiochem, stock in DMSO) to block endocytosis ...
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bioRxiv - Neuroscience 2023Quote: ... The following primary antibodies were used: polyclonal rabbit anti-tyrosine hydroxylase (TH, catalog # 657012, 1:1000, Calbiochem), monoclonal mouse anti-TH (catalog # MAB318 ...
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bioRxiv - Neuroscience 2023Quote: ... incubated overnight in TBST with 1% bovine serum albumin (BSA; Calbiochem by Millipore Sigma, St. Louis, MO) and primary antiserum (Millipore Sigma MAB318 ...
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bioRxiv - Cell Biology 2020Quote: ... 15 and 20 μM IKK-2 inhibitor IV (Calbiochem, #401484) for 6 hr in a serum-free medium ...
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bioRxiv - Biochemistry 2019Quote: ... 10 μl of the GO solution [2 mM ATP (Calbiochem), an oxygen-scavenging system (0.2% glucose ...
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bioRxiv - Biophysics 2021Quote: ... the coverslips were fibronectin-coated (2 µl/cm2, Calbiochem, 341635) for 1h and washed 3x with PBS and 1x with cell culture medium ...
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bioRxiv - Cell Biology 2021Quote: ... supplemented with 1x Phosphatase Inhibitor Cocktail Set 2 (Calbiochem 5246251) and 1x Protease Inhibitor Cocktail Set 1 (Calbiochem 539131).
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bioRxiv - Microbiology 2021Quote: ... and BAY11-7082 (Primary target: IκB-α; 2 μM Calbiochem).
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bioRxiv - Cancer Biology 2021Quote: ... 2 μM TGF-β receptor kinase Inhibitor IV (SB431542, Calbiochem), 10 μM ROCK inhibitor (Y-27632 ...
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bioRxiv - Genomics 2021Quote: ... 50 mmol/L 2-mercaptoethanol,10 mmol/L nicotinamide (Calbiochem), 5.5 mg/mL transferrin (Sigma Aldrich) ...
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bioRxiv - Neuroscience 2023Quote: ... or myristoylated Autocamtide-2-related inhibitory peptide (AIP, 5μM, Calbiochem) respectively ...
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bioRxiv - Neuroscience 2019Quote: ... diluted in KPBS containing 0.3 % Triton X-100 and incubated overnight at RT in cFos rabbit polyclonal antiserum (1:20,000; Calbiochem) diluted in KPBS containing 1 % NGS and 0.3 % Triton X-100 ...
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bioRxiv - Cell Biology 2020Quote: ... and the cells were washed with ice-cold PBS(-/-) with 1X Protease Inhibitor Cocktail Set 1 (Calbiochem 539131). The PBS was aspirated ...
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bioRxiv - Neuroscience 2020Quote: ... 14 – 17 DIV hippocampi cultures were stained live with a GluA1 antibody (GluA1 Calbiochem PC246, rabbit, 1:20) for 15 min at 37°C in a humidified chamber ...
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bioRxiv - Biochemistry 2021Quote: ... Cell pellets were sonicated in 20 mM HEPES buffer pH 7.5 containing 0.25 M sucrose and 1:100 protease inhibitor cocktail set V (Calbiochem) and centrifuged at 2,000 × g for 10 min to pellet nuclear fraction ...
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bioRxiv - Biochemistry 2021Quote: ... Data were normalized to maximal inhibition determined by 1 μM decanoyl-Arg-Val-Lys-Arg-chloromethylketone (Calbiochem #344930).
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bioRxiv - Neuroscience 2021Quote: ... or P5–P6 DRG rat neurons were diluted to 35,000 cells/ml in Sato’s media and treated with either 1 mM dbcAMP (Calbiochem), mixed population or recombinant Histones (1-20µg/ml ...
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bioRxiv - Immunology 2020Quote: ... Cells were grown at 37°C to OD = 0.8 and expression was induced with 0.3 mM IPTG (Isopropyl b-D-1-thiogalactopyranoside, Calbiochem) overnight at 18°C ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... sections were incubated with rabbit anti-c-Fos antibody diluted with the blocking solution (1:20000, #PC38, Calbiochem) for 2 days at 4°C ...
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bioRxiv - Molecular Biology 2022Quote: ... Samples were incubated for 1 h with the ADAM17 inhibitor Batimastat (BB-94) at 10 μM (196440, Calbiochem), with the selective ADAM10 inhibitor GI254023X (GI ...
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bioRxiv - Biophysics 2023Quote: ... and the C-class iPSCs with a mixture of two inhibitors (2i): 1 μM MEK inhibitor (PD0325901, Calbiochem) and 3 μM GSK3 inhibitor (CH99021 ...
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bioRxiv - Molecular Biology 2024Quote: ... The pellet was suspended in 2 ml of lysis/binding buffer (25 mM HEPES/KOH, pH 7.6, 10 % glycerol, 150 mM KCl, 1 mM dithiothreitol, 0.1 % Elugent (Calbiochem), loaded into SHREDDER tubes (Pressure Biosciences Inc ...
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bioRxiv - Genetics 2020Quote: ... A 2 mg/mL stock solution of hemin chloride from Calbiochem was prepared in 0.05 M NaOH ...
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bioRxiv - Immunology 2022Quote: ... for 4 h in the presence of 2 μM monensin (Calbiochem) during the last 2 h of stimulation ...
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bioRxiv - Plant Biology 2020Quote: ... 1.7-2% (w/v) Cellulysin (Calbiochem, Behring Diagnostics, La Jolla, CA), 0.026 % (w/v ...
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bioRxiv - Cancer Biology 2019Quote: ... MAP kinase inhibitors for ERK1/2 (FR180204) and p38 (SB202190) (Calbiochem) were supplemented in the media at 6 μM after seeding cells and changing each media ...
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bioRxiv - Immunology 2022Quote: ... and subsequently selected using 2 µg/mL puromycin (Calbiochem, Cat#540411) for 96 h ...
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bioRxiv - Cell Biology 2019Quote: ... cells were lysed at 1 × 109 cells/ml in ice-cold lysis buffer (0.1 µM TLCK, 1 µg/ml Leupeptin, Phosphatase Inhibitor Cocktail II (Calbiochem),1 mM PMSF ...
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bioRxiv - Biochemistry 2019Quote: ... which were grown to an optical density of 0.6–0.8 at 600 nm and were induced with 1 mM isopropyl-β-D-thiogalactopyranoside (CALBIOCHEM) for 20 h at 18 °C ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were rinsed with cold PBS1X and then lysed in Triton-X100 buffer at 4°C (50mM HEPES pH 7.4, 150mM NaCl, 1 % Triton X-100, protease inhibitors (Calbiochem) and phosphatase inhibitor cocktails II and IV (Calbiochem)) ...
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bioRxiv - Cell Biology 2021Quote: ... 50 μg of the total RNA was incubated for 1 h at 4 °C with 10 μl of the anti-2,2,7-trimethylguanosine-antibody (Calbiochem Milipore) coupled to sepharose beads (Fig ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were then harvested using lysis buffer (50mM HEPES pH7.4, 150mM NaCl, 1mM MgCl2, 1mM NaF, 1% Triton-x-100, protease inhibitor cocktail I [Calbiochem) and centrifuged at 14,000rpm for 10 minutes ...
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bioRxiv - Cancer Biology 2022Quote: ... collected and lysed in lysis buffer RGMT (50mM HEPES pH 7.4, 150mM NaCl, 1mM MgCl2, 1mM NaF, 1% Triton-x-100, plus protease inhibitor cocktail I [Calbiochem]). Cellular lysates were cleared by centrifugation and 225µL of total protein extract was incubated with biotin-tagged CIGB-300 (100μM ...
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bioRxiv - Molecular Biology 2022Quote: ... The cell pellet was lysed at 0.5 × 109 cells/ml in ice-cold lysis buffer (0.1 mM TLCK, 1 μg/ml Leupeptin, 1x Phosphatase Inhibitor Cocktail II tablet (Calbiochem), 1 mM PMSF ...
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bioRxiv - Developmental Biology 2022Quote: ... Tissues were homogenized in cold lysis buffer (0.1% NP-40, 50mM Tris-HCl [pH 7.2], 250mM NaCl, 2mM EDTA, phosphatase inhibitor mixture I and II [Calbiochem] and one tablet of Minicomplete [Roche] per 10ml ...
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bioRxiv - Cell Biology 2022Quote: ... alone bound to glutathione sepharose 4B beads in 0.7 ml of binding buffer (1X PBS, 150 mM NaCl, 1% Triton X-100, Calbiochem EDTA-free protease inhibitor cocktail set III ...
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bioRxiv - Immunology 2022Quote: ... and 1 × 106 cells were treated with rapamycin (10 ng/ml, 30 min, 37°C; Calbiochem, San Diego, CA) prior to treatment with rhGH.
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bioRxiv - Molecular Biology 2022Quote: ... pellets were resuspended in 800 μl of ice-cold wash buffer (50 mM NaH2PO4, 300 mM NaCl, 0.05% Tween, 1:200 diluted protease inhibitor cocktail set III (Calbiochem)) supplemented with 0.1 U/μL of recombinant RNase inhibitor (wash buffer-RIn ...
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bioRxiv - Biochemistry 2023Quote: ... Culture was then cooled to 16℃ and induced with 0.4 mM dioxane-free isopropyl B-D-1-thiogalactopyranoside (IPTG) (Calbiochem) overnight at 16℃ ...