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Citations for Calbiochem :
351 - 400 of 765 citations for 7 Chloro 3 methyl 3H 1 2 3 triazolo 4 5 d pyrimidine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... The outer membrane pellets were solubilized in Tris buffer containing 2 % Elugent (Calbiochem) at 4°C for 16 h with stirring ...
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bioRxiv - Neuroscience 2020Quote: ... 5 μg/ml RNAse A pH 7.4 and protease inhibitor cocktail set III EDTA-free (Calbiochem) added of 1 mM PMSF ...
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bioRxiv - Plant Biology 2021Quote: ... and the supernatant re-centrifuged for 5 min and filtered through a layer of Miracloth (Calbiochem). Clarified extracts were incubated with pre-washed GFP-Trap®_M magnetic beads on an end-to-end tube rotator at 4°C for 1 hr ...
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bioRxiv - Plant Biology 2021Quote: ... 5-day-old seedlings were transferred to medium supplemented with 0.6 mg/L bleomycin sulphate (Calbiochem) for 24 h.
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bioRxiv - Plant Biology 2019Quote: ... 35 μL water and 5 μL 100 mM monobromobimane in acetonitrile (Thiolyte® MB, Calbiochem, www.merckmillipore.com) were mixed and added to the samples ...
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bioRxiv - Immunology 2019Quote: ... cultured in R10 for several days and activated with 5 μg/ml phytohaemagglutinin (PHA-L, Calbiochem, Millipore Sigma ...
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bioRxiv - Physiology 2023Quote: Total protein extracts were obtained from microalgal cells in exponential phase of growth (e.g. 4 days) in 50 mM Tris buffer pH 8.0 supplemented with protein inhibitor cocktail (539131, Calbiochem). Free-living and symbiotic microalgae were disrupted by bead beating with a Precellys device (Bertin Technologies ...
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bioRxiv - Cell Biology 2023Quote: ... cells were treated for 2 h with 35 µM cycloheximide (Calbiochem, catalog no. 239763). For mTOR inhibition-dependent autophagy arrest at the initiation step ...
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bioRxiv - Cancer Biology 2019Quote: ... mice received 1 µg DT (322326-1, Calbiochem) diluted in 100 µl PBS through intraperitoneal (i.p. ...
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bioRxiv - Cancer Biology 2022Quote: ... incubated with 30 nM DAPI for 5 minutes and mounted on slides with FluorSave reagent (Calbiochem 345789). Immunofluorescence images were taken using a Keyence BZ-X800E Fluorescent Microscope with a 40X or 60X objective lens ...
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bioRxiv - Biochemistry 2021Quote: ... whereupon the final two wash steps were replaced with a single wash in 1.0 ml of 5 mM sodium phosphate buffer (pH 7.4) containing 0.1% (w/v) sarkosyl (Calbiochem) and 0.3% (w/v ...
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bioRxiv - Biochemistry 2021Quote: Uridine-5-triphosphate (UTP; cat #6701) and phorbol 12,13-dibutyrate (PDBu; cat #524390) were purchased from Calbiochem. Calyculin A (cat #9902 ...
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bioRxiv - Immunology 2021Quote: ... under the CD11c/Itgax promoter upon intraperitoneal (i.p.) injection of 4 ng/g of mouse body weight of diphtheria toxin (DT, Calbiochem) 12 hrs prior to the targeted CD11c+ cell depletion time as indicated in the relevant figure legends ...
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bioRxiv - Biochemistry 2021Quote: ... cells were trypsinized and seeded for selection in complete media supplemented with 4 μg/mL puromycin (Calbiochem, Merck Millipore). All cell lines were passaged 3 times before use and kept in culture supplemented with 2 μg/mL puromycin and/or stored in liquid nitrogen.
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bioRxiv - Plant Biology 2020Quote: ... Homogenate was centrifuged at 10,000 x g at 4°C for 10 minutes and filtered through two layers of Miracloth (Calbiochem) before centrifugation at 40,000 x g for one hour at 4°C ...
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bioRxiv - Developmental Biology 2020Quote: ... allowed to dry for 30 min at 37 °C and mounted in MOWIOL 4-88 Reagent (Calbiochem, Darmstadt, Germany).
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bioRxiv - Plant Biology 2023Quote: ... The homogenate was centrifuged at 4°C for 15 min at 10,000×g and the supernatant was filtered through Miracloth (Calbiochem) to remove plant debris ...
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bioRxiv - Microbiology 2023Quote: ... 2-day-grown conidia were collected and filtered through Miracloth (Calbiochem, San Diego, CA, USA) (66).
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bioRxiv - Neuroscience 2023Quote: ... samples were washed in PBS and 2 drops of FluorSave reagent (Calbiochem, San Diego, California) were added to tissue sections ...
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bioRxiv - Cell Biology 2024Quote: ... 0.1 % Triton X-100 in PBS or 2 % bovine serum albumin (GE Healthcare or Calbiochem), 0.01 % Tween 20 (National Diagnostics or Roth ...
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bioRxiv - Synthetic Biology 2021Quote: ... 1% (v/v) Protease inhibitor mix set 1 (Calbiochem; 539131); 1 Phosstop tablet (Sigma ...
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bioRxiv - Neuroscience 2020Quote: ... rabbit anti-Fos (1:5000, Calbiochem; 1:2000, Cell Signaling), guinea pig anti-Fluorogold (1:1000 ...
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bioRxiv - Microbiology 2021Quote: ... The conidiospores were washed by 5 mL distilled water and filtrated through two layers of Miracloth (Calbiochem, Germany). The yield of spores was measured by a hemocytometer ...
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bioRxiv - Neuroscience 2021Quote: ... allowed to dry for 30 min at 37 °C and mounted in MOWIOL® 4-88 (Calbiochem, Darmstadt, Ger-many).
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bioRxiv - Cell Biology 2019Quote: ... and incubated overnight at 4 °C with mouse monoclonal antibodies against type I collagen (20 μg/mL, CP-17, Calbiochem), type II collagen (0.4 μg/mL ...
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bioRxiv - Cell Biology 2020Quote: ... the following inhibitors were used at the indicated concentrations for 4 h unless indicated differently in the experiments: RO-3306 at 10 μM (CDK1 inhibitor; Calbiochem), NU6140 at 10 μM (CDK2 inhibitor ...
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bioRxiv - Cell Biology 2020Quote: ... Samples were labeled with the indicated primary antibodies overnight at 4°C in the presence of 0.01% saponin (Calbiochem, 558255) and 1% BSA ...
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bioRxiv - Microbiology 2021Quote: ... parasites were fixed in 4% paraformaldehyde containing 0.0075% glutaraldehyde (Nacalai Tesque, Japan) and permeabilized with PBS containing 0.1% Triton-X100 (Calbiochem, CA, USA), then blocked with PBS containing 3% BSA ...
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bioRxiv - Biochemistry 2021Quote: ... resuspended in 4 ml ice-cold KHM buffer supplemented with 80 μg/ml high purity digitonin (Calbiochem, cat. number 300410) and incubated on ice for 5 min to permeabilise the plasma membrane ...
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bioRxiv - Cancer Biology 2023Quote: ... The concentration of anti-E-A14-specific Ig in plasma was estimated in comparison with a standard curve generated with anti-Her2 monoclonal antibody (Ab-4; Calbiochem).
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bioRxiv - Neuroscience 2020Quote: ET-1 (Calbiochem) was used to induce stroke as previously described [27,49] ...
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bioRxiv - Neuroscience 2021Quote: ... InSolution Roscovitine (CAS # 186692-46-6) and Bafilomycin A1 (CAS #88899-55-2) were from Calbiochem. N6-Benzoyladenosine-3’ ...
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bioRxiv - Immunology 2023Quote: ... cells were stimulated ex vivo with PMA (10ng/ml) and ionomycin (1μM) for 4 hours in the presence of 2μM monensin (Calbiochem, San Diego, CA) during the last 2 hours of stimulation ...
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bioRxiv - Cancer Biology 2021Quote: Cell pellets were sonicated in ice-cold lysis buffer (containing 20 mM Tris-HCl, pH 7.4, 5 mM EDTA, 10 mM Na4P2O7 (Calbiochem), 100 mM NaF ...
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bioRxiv - Developmental Biology 2020Quote: ... female C57BL/6J mice (age 6–8 weeks) were superovulated by intraperitoneal injection of pregnant mare’s serum gonadotropin (PMSG; 5 U; Calbiochem) followed by human chorionic gonadotropin (hCG ...
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bioRxiv - Neuroscience 2019Quote: ... Membranes were blocked to reduce nonspecific binding using 5% milk with PBS-TWEEN® tablets (PBS-T) (Calbiochem, USA) at room temperature (RT ...
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bioRxiv - Immunology 2020Quote: ... Fixed cells were washed three times in PBS and permeabilized by incubating them in PBS containing 5% non-fat milk and 0.05% saponin (Calbiochem). Coverslips were placed in primary antibody for 1 hour then washed 3x in PBS and placed in secondary antibody ...
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bioRxiv - Neuroscience 2023Quote: ... and counterstained with DAPI (10 min) before being washed (3x 5-min PBS 0.1M) and mounted on gelatin slides and coated with FluorSave (Calbiochem). Images of the whole section were taken using Axioscan.Z1 (Zeiss ...
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bioRxiv - Biophysics 2022Quote: ... [Ca2+]ER levels were assessed by the addition of 2 µM ionomycin (IO; Calbiochem, San Diego, CA), a Ca2+ ionophore ...
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bioRxiv - Neuroscience 2021Quote: ... they were incubated for 2 h in secondary antibody (anti-mouse fluorescein conjugated, Calbiochem cat n. 401234) 1:100 in BSA 1% + sheep serum 2% in PBT ...
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bioRxiv - Immunology 2020Quote: ... Rac1 inhibitor (NSC23766) and Arp-2 complex inhibitor I (CK-666) & II (CK-869) purchased from Calbiochem were used for blocking actin assembly (33).
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bioRxiv - Immunology 2023Quote: ... NF-κB (BAY11-7082; 2 μM) and RAF1 (RAF1 Kinase inhibitor I; 10 μM) were from Calbiochem. The inhibitor of EGFR (gefitinib ...
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bioRxiv - Molecular Biology 2019Quote: ... 1 mM EDTA, 150 mM NaCl, 1% Triton X-100, 1 mM PMSF and 1X protease inhibitor cocktail III [Calbiochem]). After a 5-minute incubation on ice ...
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bioRxiv - Cell Biology 2023Quote: ... pH 7.6, 1 mM EDTA, 1% Triton X-100, 0.1% sodium deoxycholate, 1 mM DTT, and protease inhibitor cocktail [Calbiochem, #539134]). The beads were incubated in a microcentrifuge tube with 5 µg of anti-FLAG antibodies (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2023Quote: ... pH 7.6, 1 mM EDTA, 1% Triton X-100, 0.1% sodium deoxycholate, 1 mM DTT, and protease inhibitor cocktail [Calbiochem, #539134]). The beads were incubated in a microcentrifuge tube with 3 µg rabbit anti-SDC-3 (lab stock) ...
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bioRxiv - Cell Biology 2023Quote: ... pH 7.6, 1 mM EDTA, 1% Triton X-100, 0.1% sodium deoxycholate, 1 mM DTT, and protease inhibitor cocktail [Calbiochem, #539134]) for every 1 gram of animals ...
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bioRxiv - Cell Biology 2023Quote: ... pH 7.6, 1 mM EDTA, 1% Triton X-100, 0.1% sodium deoxycholate, 1 mM DTT, and protease inhibitor cocktail [Calbiochem, #539134]) for every 1 gram of embryos and homogenized with 50 strokes in a Dounce homogenizer ...
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bioRxiv - Pathology 2020Quote: ... alpha-tubulin (Abcam, ab4074 - 1:2500-5000 and Calbiochem, CP06 1:10000), Cyp4A (Abcam ...
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bioRxiv - Biochemistry 2020Quote: Necrostatin-1 (Nec-1) and necrostatin-1s (Nec-1s) were from Calbiochem (VWR International ...
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bioRxiv - Neuroscience 2021Quote: ... MyoVin-1 (Calbiochem; 475984) and 2,4,6-triiodophenol (Sigma-Aldrich ...