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Citations for Calbiochem :
301 - 350 of 706 citations for 6 4 Methoxyphenyl 2 oxo 1 2 dihydro 3 pyridinecarboxylic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: Stable mACSF2 knockdown cells and control cells were grown on glass cover slips and treated with 40 μM all-trans-retinoic acid (Calbiochem, San Diego CA). Forty eight hours post transfection cells were fixed for 20 min with a solution of 3% formaldehyde in PBS ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were again washed with PBS pH 7.4 (3 × 10 min) and mounted in antifade (Mowiol, Calbiochem).
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bioRxiv - Neuroscience 2020Quote: ... collected on a microscope slide and coverslips were mounted with Mowiol 4-88 (MilliporeSigma Calbiochem). Primary antibodies were the following ...
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bioRxiv - Developmental Biology 2022Quote: ... the slides were washed with PBS and mounted with water based Mowiol 4-88 (Calbiochem) mounting media ...
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bioRxiv - Genomics 2023Quote: ... and immunoprecipitations were carried out by adding 4 μg of the appropriate antibodies (MPO, Calbiochem) to
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bioRxiv - Biochemistry 2023Quote: ... The coverslips were then mounted in Mowiol 4-88 solution (Calbiochem/EMD Millipore, Billerica, MA) and fluorescence analysis was performed using a Plan-Apochromat 63×/1.4 objective (Zeiss ...
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bioRxiv - Genomics 2024Quote: ... Sections were counterstained with 0.25 µg/mL DAPI and mounted in Mowiol 4-88 (Calbiochem). Imaging was performed using the Nikon SMZ18 stereomicroscope and Olympus FluoView FV3000 confocal microscope ...
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bioRxiv - Microbiology 2023Quote: ... The reaction mixture contains 8 μg total proteins and 4 μg nitrocefin (Calbiochem, San Diego, CA). β-Lactamase activity was determined by measuring the absorbance at 486 nm (OD486nm).
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bioRxiv - Cell Biology 2023Quote: ... in PBS for 5 min at RT and cells were mounted in Mowiol 4-88 (Calbiochem) solution (Wei and Seemann ...
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bioRxiv - Neuroscience 2023Quote: ... and incubated at 4°C for 24hrs with primary rabbit monoclonal anti-CGRP antibody (Calbiochem PC205) diluted 1:200 in 0.4% BSA ...
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bioRxiv - Molecular Biology 2021Quote: ... The expression of recombinant PGC-1α proteins was induced for 3 h at 37°C with 0.4 mM isopropyl-β-D-thiogalactoside (IPTG, Calbiochem) in Terrific Broth and purified by metal ion affinity chromatography (IMAC ...
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bioRxiv - Cell Biology 2023Quote: ... the cells were fixed with cold methanol (10 min at −20°C) and blocked for 30 min with 3% (w/v) bovine serum albumin (Calbiochem) in 1 x PBS at room temperature ...
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bioRxiv - Pathology 2024Quote: ... NETs formation was induced for 3 hours at 37°C and 5% CO2 by stimulation with 10 µM of PAF (Calbiochem) on primed neutrophils and 25 µM on non-primed ones ...
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bioRxiv - Neuroscience 2024Quote: ... The NPCs were differentiated into neurons in the absence of bFGF for at least 3 weeks and treated with 10 μM ROCK inhibitor (Y-27632; Calbiochem) during the first 24 h.
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bioRxiv - Molecular Biology 2023Quote: ... 10 mM EDTA, 1% NP-40, 1% sodium deoxycholate, 0.1% SDS, 1 mM DTT, 5 mM PMSF, and 1:100 Calbiochem Complete Protease Inhibitor Cocktails VII and V ...
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bioRxiv - Physiology 2023Quote: Total protein extracts were obtained from microalgal cells in exponential phase of growth (e.g. 4 days) in 50 mM Tris buffer pH 8.0 supplemented with protein inhibitor cocktail (539131, Calbiochem). Free-living and symbiotic microalgae were disrupted by bead beating with a Precellys device (Bertin Technologies ...
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bioRxiv - Cancer Biology 2019Quote: ... mice received 1 µg DT (322326-1, Calbiochem) diluted in 100 µl PBS through intraperitoneal (i.p. ...
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bioRxiv - Microbiology 2022Quote: ... the preformed spheroids were transferred into the fibrin gel consisting of plasminogen-free human fibrinogen (final concentration 3 mg/ml; Calbiochem, USA) and human thrombin (final concentration 2 U/ml ...
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bioRxiv - Cell Biology 2023Quote: ... Vacuolin-1 (5 μM; Calbiochem, CAS 351986-85-1); Manumycin A (2,5 μM ...
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bioRxiv - Immunology 2021Quote: ... under the CD11c/Itgax promoter upon intraperitoneal (i.p.) injection of 4 ng/g of mouse body weight of diphtheria toxin (DT, Calbiochem) 12 hrs prior to the targeted CD11c+ cell depletion time as indicated in the relevant figure legends ...
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bioRxiv - Biochemistry 2021Quote: ... cells were trypsinized and seeded for selection in complete media supplemented with 4 μg/mL puromycin (Calbiochem, Merck Millipore). All cell lines were passaged 3 times before use and kept in culture supplemented with 2 μg/mL puromycin and/or stored in liquid nitrogen.
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bioRxiv - Plant Biology 2020Quote: ... Homogenate was centrifuged at 10,000 x g at 4°C for 10 minutes and filtered through two layers of Miracloth (Calbiochem) before centrifugation at 40,000 x g for one hour at 4°C ...
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bioRxiv - Developmental Biology 2020Quote: ... allowed to dry for 30 min at 37 °C and mounted in MOWIOL 4-88 Reagent (Calbiochem, Darmstadt, Germany).
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bioRxiv - Plant Biology 2023Quote: ... The homogenate was centrifuged at 4°C for 15 min at 10,000×g and the supernatant was filtered through Miracloth (Calbiochem) to remove plant debris ...
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bioRxiv - Synthetic Biology 2021Quote: ... 1% (v/v) Protease inhibitor mix set 1 (Calbiochem; 539131); 1 Phosstop tablet (Sigma ...
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bioRxiv - Neuroscience 2020Quote: ... rabbit anti-Fos (1:5000, Calbiochem; 1:2000, Cell Signaling), guinea pig anti-Fluorogold (1:1000 ...
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bioRxiv - Neuroscience 2021Quote: ... allowed to dry for 30 min at 37 °C and mounted in MOWIOL® 4-88 (Calbiochem, Darmstadt, Ger-many).
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bioRxiv - Cell Biology 2019Quote: ... and incubated overnight at 4 °C with mouse monoclonal antibodies against type I collagen (20 μg/mL, CP-17, Calbiochem), type II collagen (0.4 μg/mL ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were washed twice with 3 ml PBS at 400 g for 5 minutes at 4°C and 500 uL PI staining buffer was added (1X PBS, 50 μg/mL PI (Calbiochem), 2μg/mL RNase A (Sigma ...
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bioRxiv - Cell Biology 2020Quote: ... the following inhibitors were used at the indicated concentrations for 4 h unless indicated differently in the experiments: RO-3306 at 10 μM (CDK1 inhibitor; Calbiochem), NU6140 at 10 μM (CDK2 inhibitor ...
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bioRxiv - Cell Biology 2020Quote: ... Samples were labeled with the indicated primary antibodies overnight at 4°C in the presence of 0.01% saponin (Calbiochem, 558255) and 1% BSA ...
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bioRxiv - Microbiology 2021Quote: ... parasites were fixed in 4% paraformaldehyde containing 0.0075% glutaraldehyde (Nacalai Tesque, Japan) and permeabilized with PBS containing 0.1% Triton-X100 (Calbiochem, CA, USA), then blocked with PBS containing 3% BSA ...
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bioRxiv - Biochemistry 2021Quote: ... resuspended in 4 ml ice-cold KHM buffer supplemented with 80 μg/ml high purity digitonin (Calbiochem, cat. number 300410) and incubated on ice for 5 min to permeabilise the plasma membrane ...
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bioRxiv - Neuroscience 2023Quote: ... O-GlcNAc transferase and O-GlcNAcase activities were blocked by 5 µM OSMI-4 (MedChem Express) and 5 µM Thiamet-G (Calbiochem) treatments ...
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bioRxiv - Cancer Biology 2023Quote: ... The concentration of anti-E-A14-specific Ig in plasma was estimated in comparison with a standard curve generated with anti-Her2 monoclonal antibody (Ab-4; Calbiochem).
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bioRxiv - Neuroscience 2020Quote: ET-1 (Calbiochem) was used to induce stroke as previously described [27,49] ...
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bioRxiv - Biophysics 2022Quote: The activity of β-galactosidase was measured using a spectrophotometric assay in which o-Nitrophenyl-β-D-Galactopyranoside (ONPG, CAS Nr: 369-07-3 Calbiochem Sigma-Aldrich) was used as a substrate ...
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bioRxiv - Immunology 2023Quote: ... cells were stimulated ex vivo with PMA (10ng/ml) and ionomycin (1μM) for 4 hours in the presence of 2μM monensin (Calbiochem, San Diego, CA) during the last 2 hours of stimulation ...
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bioRxiv - Biochemistry 2020Quote: ... 1 µmol L-1 pepstatin A and 5 µmol L-1 phenyl-methane-sulfonyl-fluoride) was from Calbiochem (Darmstadt, Germany). Ammonium sulfate-depleted PK ...
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bioRxiv - Molecular Biology 2019Quote: ... 1 mM EDTA, 150 mM NaCl, 1% Triton X-100, 1 mM PMSF and 1X protease inhibitor cocktail III [Calbiochem]). After a 5-minute incubation on ice ...
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bioRxiv - Pathology 2020Quote: ... alpha-tubulin (Abcam, ab4074 - 1:2500-5000 and Calbiochem, CP06 1:10000), Cyp4A (Abcam ...
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bioRxiv - Biochemistry 2020Quote: Necrostatin-1 (Nec-1) and necrostatin-1s (Nec-1s) were from Calbiochem (VWR International ...
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bioRxiv - Neuroscience 2021Quote: ... MyoVin-1 (Calbiochem; 475984) and 2,4,6-triiodophenol (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2019Quote: ... purmorphamine 1 μM (Calbiochem), SAG 1 μM (Calbiochem) ...
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bioRxiv - Neuroscience 2019Quote: ... SAG 1 μM (Calbiochem), IGF-1 10 ng/ml (PeproTech) ...
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bioRxiv - Cell Biology 2019Quote: ... containing 1% Digitonin (Calbiochem) in 50 mM Tris-HCl ...
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bioRxiv - Immunology 2021Quote: ... 1 mM GSK872 (Calbiochem), For necroptosis induction cells were treated with 100ng/ml TNF-α (Peprotech ...
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bioRxiv - Neuroscience 2023Quote: ... Primary antibodies and dilution factors were as follows: rabbit anti-TH (1:40000 for SNpc and 1:3500 for striatum immunochemistry; 1:1000 for immunofluorescence, Calbiochem, Cat#657012); mouse anti-GFAP (1:1000 ...
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bioRxiv - Cell Biology 2020Quote: Proteasome activity was quantitatively assessed in H4 cell extracts using the β5-selective fluorogenic substrate succinyl-leucine-leucine-valine-tyrosine-4-methyl-7-courmarylamide (Suc-LLVY-AMC, Calbiochem, Burlington, MA, USA) using an adapted protocol 87 ...
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bioRxiv - Cell Biology 2020Quote: ... for 1 hr followed by 1:500 dilution of anti-mouse Alexa-594 (Calbiochem) for 45 min at room temperature ...