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Citations for Calbiochem :
201 - 250 of 287 citations for Mouse Two pore calcium channel protein 2 Tpcn2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... after which the gels was washed with water and all protein was stained using RAPIDstain (Calbiochem, 553215) for 1 hour ...
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bioRxiv - Neuroscience 2022Quote: ... the retinas were treated with the primary antibodies (1000x + 1000x mouse SMI31 + SMI32 = SMI312, NE1022 / NE1023 - Calbiochem; 1000x rabbit Caspase3 ...
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bioRxiv - Molecular Biology 2023Quote: ... The lower half of the membrane was probed with mouse anti-GAPDH (Calbiochem clone 6C5 CB1001-500UG) at 1:10,000 while the upper half was probed with mouse anti-FLAG (Sigma-Aldrich/Millipore F1804-1MG ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: Brain tissue was homogenized in RIPA buffer supplemented with protease and protein phosphatase inhibitors (Calbiochem, La Jolla, CA) and subjected to SDS-PAGE followed by transferring to PVDF membranes (Millipore ...
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bioRxiv - Physiology 2023Quote: Total protein extracts were obtained from microalgal cells in exponential phase of growth (e.g. 4 days) in 50 mM Tris buffer pH 8.0 supplemented with protein inhibitor cocktail (539131, Calbiochem). Free-living and symbiotic microalgae were disrupted by bead beating with a Precellys device (Bertin Technologies ...
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bioRxiv - Cancer Biology 2019Quote: ... Membranes were incubated in 1:1000 diluted primary antibodies pERK1/2 pT202/Y204 (Calbiochem cat# KP26001), total ERK1/2 (Cell Signaling cat# 9102) ...
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bioRxiv - Neuroscience 2021Quote: ... InSolution Roscovitine (CAS # 186692-46-6) and Bafilomycin A1 (CAS #88899-55-2) were from Calbiochem. N6-Benzoyladenosine-3’ ...
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bioRxiv - Immunology 2023Quote: ... 2 µl were used as PCR template for amplification with the KOD Polymerase (Calbiochem, 71086-3). The PCR products were cloned into the pI.18 vector ...
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bioRxiv - Molecular Biology 2019Quote: The ProteoExtract® Subcellular Proteome Extraction Kit (Calbiochem) was used to generate lysates from the cytosolic ...
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bioRxiv - Molecular Biology 2020Quote: ... or with polyclonal anti-Sir3 antibodies and immune complexes were collected with Protein G/A beads (Calbiochem, EMD Biosciences). Immunoprecipitated and input DNA were purified using Chelex 100 (Bio-Rad ...
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bioRxiv - Molecular Biology 2020Quote: ... Pellets were resuspended in 300 µl of DMEM 2% FBS 1 µg/mL propidium iodide (Calbiochem, 537060) and filtered through a 40-µm cell strainer (BD Falcon ...
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bioRxiv - Biophysics 2022Quote: ... [Ca2+]ER levels were assessed by the addition of 2 µM ionomycin (IO; Calbiochem, San Diego, CA), a Ca2+ ionophore ...
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bioRxiv - Molecular Biology 2020Quote: ... Lyophilized MEISi-1 and MEISi-2 small molecules were dissolved in sterile dimethyl sulfoxide (DMSO, Calbiochem, 317275). Luciferase reporter assays were carried out as we have done previously 13,14,16,17 ...
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bioRxiv - Immunology 2020Quote: ... Rac1 inhibitor (NSC23766) and Arp-2 complex inhibitor I (CK-666) & II (CK-869) purchased from Calbiochem were used for blocking actin assembly (33).
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bioRxiv - Microbiology 2023Quote: ... 3-(2,4-dinitrostyryl)-(6 R,7 R)-7-(2-thienylacetamido)-ceph-3-em-4-carboxyl acid (Calbiochem), was used to assess membrane permeability ...
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bioRxiv - Neuroscience 2023Quote: Cortical neurons were incubated with 4 μM fura-2 acetoxymethyl ester (AM, Calbiochem, CAS 108964-32-5) 40 min at 37°C ...
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bioRxiv - Immunology 2023Quote: ... NF-κB (BAY11-7082; 2 μM) and RAF1 (RAF1 Kinase inhibitor I; 10 μM) were from Calbiochem. The inhibitor of EGFR (gefitinib ...
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bioRxiv - Immunology 2021Quote: ... under the CD11c/Itgax promoter upon intraperitoneal (i.p.) injection of 4 ng/g of mouse body weight of diphtheria toxin (DT, Calbiochem) 12 hrs prior to the targeted CD11c+ cell depletion time as indicated in the relevant figure legends ...
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bioRxiv - Plant Biology 2021Quote: ... The chromatin complexes were immunoprecipitated with anti-GFP antibody (ab290) and an anti-myc mouse antibody (mAb 9E10, Calbiochem) with a concentration of 2 μg / sample and incubated in ChIP dilution buffer with 20 μL of Dynabeads Protein A (Thermofisher ...
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bioRxiv - Immunology 2021Quote: CCR2+ and CD11c+ cells were depleted from diphtheria toxin receptor (DTR)-expressing mice upon intraperitoneal (i.p.) injection of 10 ng/g of mouse body weight of diphtheria toxin (DT, Calbiochem) 12 hrs prior to Lm challenge infection.
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bioRxiv - Microbiology 2019Quote: ... Lysates were cleared by centrifugation and precipitated with anti-HA antibody (clone HA-7; Bioshop) using agarose protein G beads (Calbiochem) as previously described25 ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins were extracted using an 8M urea lysis buffer (8M urea, 50 mM Tris-HCl, 75 mM NaCl, 1x Calbiochem protease inhibitor ...
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bioRxiv - Plant Biology 2019Quote: ... After 30 min incubation on a rotator at 4°C the protein samples were centrifuged at 18,000xg for 30 min to remove plant debris and the protein extract was filtered through Miracloth filter paper (Calbiochem Co.). Next ...
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bioRxiv - Molecular Biology 2021Quote: ... The expression of recombinant PGC-1α proteins was induced for 3 h at 37°C with 0.4 mM isopropyl-β-D-thiogalactoside (IPTG, Calbiochem) in Terrific Broth and purified by metal ion affinity chromatography (IMAC ...
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bioRxiv - Biochemistry 2022Quote: ... lysed in M-PER mammalian protein extraction reagent (Thermo-Fisher) with 1/1000 of the EDTA-free protease inhibitor cocktail Set III (Calbiochem), and centrifuged for 30 min at 15,000 × g at 4°C ...
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bioRxiv - Molecular Biology 2020Quote: ... 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reagent was obtained from Calbiochem (San Diego, CA, USA).
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bioRxiv - Physiology 2021Quote: ... and phosphatase inhibitors (2 μM microcystin-LR, and 1× Phosphatase-Inhibitor Sets I and II (Calbiochem, Hayward, CA). We centrifuged homogenates at 4,000 g for 15 minutes to remove debris ...
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bioRxiv - Pathology 2023Quote: ... 2% DDM) supplemented with 1:1000 (v/v) Protease Inhibitor Cocktail Set III (Calbiochem, La Jolla, CA, USA). Rat anti-HA (3F10 ...
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bioRxiv - Cell Biology 2019Quote: ... and incubated overnight at 4 °C with mouse monoclonal antibodies against type I collagen (20 μg/mL, CP-17, Calbiochem), type II collagen (0.4 μg/mL ...
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bioRxiv - Microbiology 2020Quote: ... Samples were incubated at 30°C for 1 h prior to SDS-PAGE and Western blotting with a mouse mono- and polyubiquitinated conjugate (Ubiquigent) or rabbit CHIP antibody (Calbiochem) with HRP-conjugated anti-mouse (GE Healthcare ...
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bioRxiv - Immunology 2019Quote: ... Sections were then incubated overnight in (4°C) with anti-c-ErbB2/c-Neu (Ab-3) mouse mAb (3B5) (1:500, Calbiochem). After washing with PBS ...
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bioRxiv - Immunology 2019Quote: ... bovine serum albumin in 0.1 M phosphate buffer for 1 h at room temperature and incubated overnight (4°C) with anti-c-ErbB2/c-Neu (Ab-3) mouse mAb (3B5) (1:500, Calbiochem) or anti-CD3 (1:500 ...
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bioRxiv - Cell Biology 2021Quote: Commercially available antibodies against the following proteins were used for Western blotting in the indicated dilutions: BRCA2 (OP95, Calbiochem, 1:1000), RAD51 (70-001 ...
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bioRxiv - Cancer Biology 2021Quote: ... and 2 mM glutamine for 24 h prior to treatment with either TGF-β (1 ng/mL, Millipore Calbiochem) or 10 uM TGF-β inhibitors (SB431542 ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... and the phenylacetamide compound (S)-2-(4-chlorophenyl)-3,3-dimethyl-N-(5-phenylthiazol-2-yl)butanamide (PA;Cmp58 (Wang et al., 2010)) was obtained from Calbiochem-Merck Millipore (Billerica ...
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bioRxiv - Physiology 2021Quote: ... cells were treated with poly(I:C) (1.5 μg/mL for 4 hrs) and a combination of proteasome inhibitor (Bortezomib, 2 μM, Sigma/Calbiochem 5043140001) and prolyl endopeptidase inhibitors (Pramiracetam 10 uM ...
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bioRxiv - Molecular Biology 2022Quote: ... Chronic low-dose inflammation was mimicked in 3T3-L1 adipocytes by incubation with 2 ng/mL TNFα (TNF; Calbiochem) for 4 d ...
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bioRxiv - Plant Biology 2022Quote: ... except that the resuspension of protein precipitate was in modified denaturing urea buffer (8 M urea, 2% SDS, 100 mM HEPES-KOH pH 7.4, 10 mM EDTA and protease inhibitor [Calbiochem, now Merck ...
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bioRxiv - Microbiology 2022Quote: ... two reactions with increasing dosages of the PfCKII-α-specific inhibitors 4,5,6,7-tetramethylbromobenzotriazole and 4,5,6,7-tetramethylbromo-2-azabenzimidazole (TBB) (Merck/Calbiochem CAS 17374-26-4) were performed to check for possible suppression of phosphoryl group transfer ...
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bioRxiv - Microbiology 2023Quote: ... Plates were then incubated at 37 °C for 2 h with different quantities (0.125 - 4 pmol) of biotinylated PfPP1c labeled with biotin-NHS (Calbiochem) in PBS-Tween 0.1% ...
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bioRxiv - Physiology 2023Quote: ... Fura-2 AM and BAPTA-AM (Cat# F1221, Molecular Probes, Eugene, OR, USA and CAS# 126150-97-8, Calbiochem San Diego ...
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bioRxiv - Biochemistry 2023Quote: ... Ubiquitin was expressed as described above except that the 2 L culture was induced with 0.4 mM dioxane-free isopropyl B-D-1-thiogalactopyranoside (IPTG) (Calbiochem) for four hrs at 37℃ ...
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bioRxiv - Cell Biology 2023Quote: The antibodies listed in Tables 1 and 2 were diluted as indicated in PBS containing 1% BSA (126575, Calbiochem).
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bioRxiv - Cell Biology 2023Quote: ... The homogenate was then incubated on ice for 5 minutes and filtered through 2 layers of Miracloth tissue (Calbiochem). The filtered homogenate was then centrifuged for 20 min at 480 rpm at 4°C to pellet any debris ...
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bioRxiv - Cancer Biology 2023Quote: NMDI-14 inhibitor (Ethyl 2-(((6,7-dimethyl-3-oxo-1,2,3,4-tetrahydro-2-quinoxalinyl)acetyl)amino)-4,5-dimethyl-3-thiophenecarboxylate) was purchased from Calbiochem (Cat. Nr. 530838) and reconstituted in DMSO (10mg/ml ...
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bioRxiv - Cell Biology 2023Quote: ... The antibodies (Tables 2 and 3) were diluted according to the tables in PBS containing 1% BSA (126575, Calbiochem).
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bioRxiv - Genomics 2019Quote: ... and SV40 large T antigen of the BK virus (Affinity purified and agarose conjugated IgG2A mouse monoclonal antibody recognizing the 94kDa SV40 large T antigen; PAb416, Cat. No. DPO2, Calbiochem, USA) respectively.
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bioRxiv - Genetics 2023Quote: ... Raw values were normalized by geometric mean and used for subsequent housekeeping normalization (α-tubulin values; 1:10,000 mouse anti-tubulin; Calbiochem CP06) from the same blot.
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bioRxiv - Cell Biology 2022Quote: ... wild-type mouse testes (n=3 per IP assay) were lysed in 1% NP-40 (PBS) supplemented with protease inhibitor cocktail (Calbiochem, 539134). KATNA1 ...
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bioRxiv - Physiology 2023Quote: ... Normal goat serum was used as a blocking agent and slides were incubated in biotinylated hyaluronan binding protein (HABP, 1:500, CalBiochem, Burlington, MA) for one hour ...