Labshake search
Citations for Calbiochem :
201 - 250 of 614 citations for 4 Methoxy 2 3 3 4 5 Pentacb 13C12 99% 50 Ug Ml In Toluene since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Plant Biology 2023Quote: ... The homogenate was centrifuged at 4°C for 15 min at 10,000×g and the supernatant was filtered through Miracloth (Calbiochem) to remove plant debris ...
-
bioRxiv - Immunology 2021Quote: ... GMDCs were incubated for 2 h at 37°C with 50 µM MARK inhibitor (MARK/Par-1 Activity Inhibitor, 39621; Calbiochem), 50 nM SIK inhibitor (HG-9-91-01 ...
-
bioRxiv - Neuroscience 2021Quote: ... allowed to dry for 30 min at 37 °C and mounted in MOWIOL® 4-88 (Calbiochem, Darmstadt, Ger-many).
-
bioRxiv - Neuroscience 2019Quote: ... or both hippocampi from a single mouse was homogenized in 2 mL of ice-cold homogenization buffer (0.32 M sucrose, 4 mM HEPES pH 7.4, protease inhibitor cocktail EGTA-free (Calbiochem, 1:1000), RNasin (Promega ...
-
bioRxiv - Immunology 2021Quote: The caspase-1 activity was determined with the fluorometric substrate Z-YVAD 7-Amido-4-trifluoromethylcoumarin (Z-YVAD-AFC, caspase-1 substrate VI, Calbiochem), as described previously (Angosto et al. ...
-
bioRxiv - Cell Biology 2020Quote: ... the following inhibitors were used at the indicated concentrations for 4 h unless indicated differently in the experiments: RO-3306 at 10 μM (CDK1 inhibitor; Calbiochem), NU6140 at 10 μM (CDK2 inhibitor ...
-
bioRxiv - Cell Biology 2020Quote: ... Samples were labeled with the indicated primary antibodies overnight at 4°C in the presence of 0.01% saponin (Calbiochem, 558255) and 1% BSA ...
-
bioRxiv - Immunology 2022Quote: The caspase-1 activity was determined with the fluorometric substrate Z-YVAD 7-Amido-4-trifluoromethylcoumarin (Z-YVAD-AFC, caspase-1 substrate VI, Calbiochem), as described previously (Angosto et al. ...
-
bioRxiv - Microbiology 2021Quote: ... parasites were fixed in 4% paraformaldehyde containing 0.0075% glutaraldehyde (Nacalai Tesque, Japan) and permeabilized with PBS containing 0.1% Triton-X100 (Calbiochem, CA, USA), then blocked with PBS containing 3% BSA ...
-
bioRxiv - Immunology 2022Quote: Caspase-1 activity was determined with the fluorometric substrate Z-YVAD 7-Amido-4-trifluoromethylcoumarin (Z-YVAD-AFC, caspase-1 substrate VI, Calbiochem) as described previously [47,48] ...
-
bioRxiv - Biochemistry 2022Quote: ... Nuclei were stained with DAPI (1:1000 in PBS, AppliChem, Darmstadt, Germany) and coverslips were mounted on Mowiol 4-88 (Calbiochem, Merck Millipore ...
-
bioRxiv - Cancer Biology 2023Quote: ... The concentration of anti-E-A14-specific Ig in plasma was estimated in comparison with a standard curve generated with anti-Her2 monoclonal antibody (Ab-4; Calbiochem).
-
bioRxiv - Neuroscience 2023Quote: ... we used a pipette solution containing 50-100 ng/ml amphotericin B as permeabilizing agent (Calbiochem, Madrid, Spain) and a pipette-filling solution containing (in mM) ...
-
bioRxiv - Biophysics 2022Quote: The activity of β-galactosidase was measured using a spectrophotometric assay in which o-Nitrophenyl-β-D-Galactopyranoside (ONPG, CAS Nr: 369-07-3 Calbiochem Sigma-Aldrich) was used as a substrate ...
-
bioRxiv - Immunology 2022Quote: ... at a 4:1 molar ratio of monomer:streptavidin in the presence of a 1x protease inhibitor cocktail solution (Calbiochem, Millipore Sigma). 1/5th volumes of streptavidin-PE or streptavidin-BV421 were added to each monomer every 20 minutes and incubated ...
-
bioRxiv - Neuroscience 2022Quote: ... the cells were then incubated overnight at 4°C with primary antibodies (rabbit anti-AUTS2, 1:200, Atlas Antibodies; rabbit anti-GRIA1, 1:10, Calbiochem; mouse anti-GRIA2 ...
-
bioRxiv - Immunology 2023Quote: ... at a 4:1 molar ratio of monomer:streptavidin in the presence of a 1x protease inhibitor cocktail solution (Calbiochem, Millipore Sigma). Briefly ...
-
bioRxiv - Neuroscience 2020Quote: ... 5 μg/ml RNAse A pH 7.4 and protease inhibitor cocktail set III EDTA-free (Calbiochem) added of 1 mM PMSF ...
-
bioRxiv - Immunology 2019Quote: ... cultured in R10 for several days and activated with 5 μg/ml phytohaemagglutinin (PHA-L, Calbiochem, Millipore Sigma ...
-
bioRxiv - Microbiology 2021Quote: ... to OD600 1 were incubated with 750 μl of lysis buffer (100 mM EDTA [pH 8.0], 1 mM MgCl2, 5 μg/ml lysostaphin, 10 μg/ml DNase, proteinase inhibitor [cocktail set III from Calbiochem] diluted 1:100 in PBS) for 30 min at 37°C and homogenized three times with 500 μl zirconia-silica beads (0.1 mm diameter from Carl-Roth ...
-
bioRxiv - Microbiology 2021Quote: ... LY294002 (Calbiochem, 50 μM); IWP-2 (Calbiochem ...
-
bioRxiv - Neuroscience 2019Quote: ... GM6001 (Calbiochem, 50 μM), MMP9 inhibitor I (Calbiochem,10 μM).
-
bioRxiv - Molecular Biology 2022Quote: ... 50 µM PD98059 (Calbiochem) or vehicle was added to the cells for 1 h ...
-
bioRxiv - Bioengineering 2019Quote: ... with 10% FBS media (500 ml of MEM, 2.5 g D-glucose, 100 mg NaHCO3, 50 mg transferrin (Calbiochem 616420), 50 ml FBS (heat inactivated ...
-
bioRxiv - Pharmacology and Toxicology 2019Quote: ... and the phenylacetamide compound (S)-2-(4-chlorophenyl)-3,3-dimethyl-N-(5-phenylthiazol-2-yl)butanamide (PA;Cmp58 (Wang et al., 2010)) was obtained from Calbiochem-Merck Millipore (Billerica ...
-
bioRxiv - Cell Biology 2023Quote: ... The homogenate was then incubated on ice for 5 minutes and filtered through 2 layers of Miracloth tissue (Calbiochem). The filtered homogenate was then centrifuged for 20 min at 480 rpm at 4°C to pellet any debris ...
-
bioRxiv - Biochemistry 2021Quote: ... whereupon the final two wash steps were replaced with a single wash in 1.0 ml of 5 mM sodium phosphate buffer (pH 7.4) containing 0.1% (w/v) sarkosyl (Calbiochem) and 0.3% (w/v ...
-
bioRxiv - Molecular Biology 2023Quote: ... the cells were solubilized for 1 h at 4□°C in buffer (50□mM HEPES-NaOH [pH 7.5], 300□mM NaCl, 2% (w/v) N-dodecyl β-d-maltoside (DDM, Calbiochem, San Diego, CA), protease inhibitor cocktail ...
-
bioRxiv - Cell Biology 2020Quote: Proteasome activity was quantitatively assessed in H4 cell extracts using the β5-selective fluorogenic substrate succinyl-leucine-leucine-valine-tyrosine-4-methyl-7-courmarylamide (Suc-LLVY-AMC, Calbiochem, Burlington, MA, USA) using an adapted protocol 87 ...
-
bioRxiv - Cell Biology 2019Quote: ... Crosses were performed by random spore analysis (1, 2) followed by marker selection (Hygromycin/Kanamycin resistance, ura/leu auxotrophy, or sensitivity to 5 µM 1NM-PP1 (Calbiochem) for strains carrying the Ark1-as3 allele ...
-
bioRxiv - Cell Biology 2022Quote: ... the P2Y2R antagonist AR-C118925 {5-[[5-(2,8-dimethyl-5Hdibenzo[α,d]cyclohepten-5-yl)-3,4-dihydro-2-oxo-4-thioxo-1(2H)-pyrimidinyl]methyl]-A-2H-tetrazol-5-yl-2-furan-carboxamide},and the Ca2+ probes thapsigargin and ionomycin were obtained from Calbiochem-Merck Millipore (Billerica ...
-
bioRxiv - Cell Biology 2020Quote: ... 50 µM NSC23766 (Calbiochem, #553502), 15 µM (±)-Blebbistatin (BioVision ...
-
bioRxiv - Synthetic Biology 2020Quote: ... and 50 nM rapamycin (Calbiochem) was added at the indicated time.
-
bioRxiv - Microbiology 2021Quote: ... The conidiospores were washed by 5 mL distilled water and filtrated through two layers of Miracloth (Calbiochem, Germany). The yield of spores was measured by a hemocytometer ...
-
bioRxiv - Neuroscience 2020Quote: ... At 4 hours post fertilisation (hpf) embryos were treated with 100µM DAPT (In solution™ γ-secretase inhibitor IX, Calbiochem, San Diego, CA, USA) in 1% DMSO in E3 medium ...
-
bioRxiv - Molecular Biology 2020Quote: ... Pellets were resuspended in 300 µl of DMEM 2% FBS 1 µg/mL propidium iodide (Calbiochem, 537060) and filtered through a 40-µm cell strainer (BD Falcon ...
-
bioRxiv - Developmental Biology 2020Quote: ... or 2 μM IWP-2 (Calbiochem) were added for 24 or 48 hours before fixation.
-
bioRxiv - Cell Biology 2021Quote: ... anti-MMP2 (1:50,Calbiochem,USA) and anti-mouse Alexa 488 (1:500,ThermoFisher,USA) ...
-
bioRxiv - Genetics 2020Quote: ... rabbit αRAD51 PC130 (1:50, Calbiochem), rabbit αRPA serum “Moll” (1:30 ...
-
bioRxiv - Molecular Biology 2023Quote: ... and 50 µM PR-619 (Calbiochem).
-
bioRxiv - Molecular Biology 2023Quote: ... and 50 µM PR-619 (Calbiochem).
-
bioRxiv - Cell Biology 2022Quote: ... 2-aminoethoxydiphenyl borate (2-APB) from Calbiochem was purchased from Merck Quí mica Argentina SAIC (Buenos Aires ...
-
bioRxiv - Systems Biology 2022Quote: ... respectively) were resuspended in 1 mL of ChIP buffer containing 5 μl of protease inhibitor cocktail (CalbioChem, cat # 539134-1) (50 mM Tris-HCl pH 8 ...
-
bioRxiv - Neuroscience 2022Quote: ... we gently separated the retina from sclera and RPE using fine forceps or electrically-sharpened tungsten electrodes (74) and immediately started incubation in papain solution (5 U/mL papain Calbiochem#5125 ...
-
bioRxiv - Cancer Biology 2021Quote: ... and 2 mM glutamine for 24 h prior to treatment with either TGF-β (1 ng/mL, Millipore Calbiochem) or 10 uM TGF-β inhibitors (SB431542 ...
-
Proteomics of broad deubiquitylase inhibition unmasks redundant enzyme function to reveal substratesbioRxiv - Biochemistry 2020Quote: Interphase extract was prepared as previously described(Murray AW 1991) but using 2 μg/ml calcium ionophore (A23187, Calbiochem) for egg activation ...
-
bioRxiv - Molecular Biology 2022Quote: ... Chronic low-dose inflammation was mimicked in 3T3-L1 adipocytes by incubation with 2 ng/mL TNFα (TNF; Calbiochem) for 4 d ...
-
bioRxiv - Genomics 2021Quote: ... 50 mg/L hypoxanthine (Calbiochem, Sigma-Aldrich), 25 mM HEPES (Corning ...
-
bioRxiv - Cell Biology 2021Quote: ... the Ezrin Inhibitor NSC668394 (50 μM, Calbiochem), Y27632 (25 μM ...
-
bioRxiv - Cell Biology 2022Quote: ... cathepsin D (EM 1:50, #219361, Calbiochem), CD63/Lamp3 (EM 1:20 ...