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Citations for Calbiochem :
151 - 200 of 316 citations for Mouse NLR Family CARD Domain Containing 4 NLRC4 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: For IPA-3 (p21-Activated Kinas Inhibitor III, CAS 42521-82-4, Calbiochem), between 20 to 30 cells were first selected and optogenetically activated for 30 minutes ...
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bioRxiv - Immunology 2023Quote: ... for 4 h in the presence of 2μM monensin (Calbiochem, San Diego, CA) during the last 2 h of stimulation ...
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bioRxiv - Microbiology 2020Quote: mouse anti-α-tubulin (Calbiochem CP06, dilution 1:500 in immunoblot), rabbit anti-mitochondrial outer membrane translocase 20 (TOM20 ...
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bioRxiv - Microbiology 2021Quote: ... 50 μL mouse monoclonal anti-6X His tagged primary antibody (Calbiochem) was added at a dilution of 1:150 in 0.5 % BSA in PBST in the respective wells and incubated for 2 hours without shaking at room temperature ...
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bioRxiv - Cancer Biology 2021Quote: ... and mouse anti-N-Myc (NCM II 100, Calbiochem, 1:20). Counterstaining ...
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bioRxiv - Genetics 2020Quote: ... The following antibodies were used: mouse anti-KDEL (Calbiochem, 1:500), mouse anti-GM-130 (BD Transduction Laboratories ...
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bioRxiv - Microbiology 2021Quote: ... GAPDH was detected using mouse monoclonal anti-GAPDH antibody 6C5 (Calbiochem), and biotinylated proteins were detected using streptavidin-HRP (Invitrogen S911) ...
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bioRxiv - Cancer Biology 2021Quote: ... and then lysed with radio-immunoprecipitation assay (RIPA) buffer containing protease and phosphatase inhibitor cocktail (Calbiochem). The protein concentrations of cell lysate supernatants were assayed using a BCA protein assay kit (Thermo Scientific) ...
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bioRxiv - Neuroscience 2021Quote: ... hEBs were cultured in hEB medium containing 1 μM RA and 1 μM purmorphamine (Calbiochem, Germany), and the medium was changed every 2–3 days ...
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bioRxiv - Cell Biology 2019Quote: ... 1 mM EDTA) containing a protease inhibitor mixture (1:1,000; Protease Inhibitor Cocktail set III; Calbiochem) for about 5 min and cut into small pieces ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were treated for 4 h with 50 nM of bafilomycin A1 (Calbiochem, 196000) and 1 µM of bortezomib (Adooq Bioscience ...
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bioRxiv - Cell Biology 2020Quote: ... in PBS at room temperature and cells were mounted in Mowiol 4-88 (Calbiochem) embedding solution (Wei and Seemann ...
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bioRxiv - Cell Biology 2021Quote: ... samples were washed with water and mounted on coverslips with Mowiol 4-88 (Calbiochem). Images were acquired using Nyquist criterion with a Leica TCS SPE confocal system with a 63x ACS APO/ NA 1.3 oil immersion lens and galvanometer driven stage insert and deconvolved using Huygens software.
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bioRxiv - Cell Biology 2019Quote: ... cells were resuspended in KHM buffer containing CaCl2 (1 mM) and treated with Stapylocossus aureus nuclease (Calbiochem) (150 U/ml ...
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bioRxiv - Biochemistry 2021Quote: ... whereupon the final two wash steps were replaced with a single wash in 1.0 ml of 5 mM sodium phosphate buffer (pH 7.4) containing 0.1% (w/v) sarkosyl (Calbiochem) and 0.3% (w/v ...
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bioRxiv - Biophysics 2023Quote: Pharmacologically treated cells were respectively incubated in OM containing 500 nM latrunculin B (Calbiochem, Millipore, Billerica, MA), 20 U/mL cholesterol oxidase (Calbiochem ...
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bioRxiv - Cell Biology 2021Quote: ... Mouse monoclonal antibodies against human Hsp90α (CA1023) purchased from Calbiochem (Billerica, MA) and Stressmarq Biosciences (Victoria ...
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bioRxiv - Neuroscience 2020Quote: ... unless staining involved the use of mouse anti-CC1 (1:100 Calbiochem), in which case antibodies were diluted in Tris buffered saline (TBS ...
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bioRxiv - Microbiology 2019Quote: ... and GAPDH was detected using mouse monoclonal anti-GAPDH antibody 6C5 (Calbiochem). Horseradish peroxidase (HRP ...
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bioRxiv - Cancer Biology 2021Quote: ... Antibodies were: mouse anti-N-Myc (NCM II 100, Calbiochem, 1:100); rabbit anti-Lamin B1 (ab16048 ...
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bioRxiv - Cell Biology 2020Quote: ... stained with 1:600 dilution of secondary anti-mouse Alexa 647 (Calbiochem), and then washed three times ...
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bioRxiv - Cancer Biology 2023Quote: ... and incubated in primary antibodies for PCNA (mouse, 1:200, Calbiochem #PC10) and γH2AX (rabbit ...
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bioRxiv - Cell Biology 2023Quote: ... LAMP1 (rat, Developmental Studies Hybridoma Bank 1D4B) and Tubulin-□ (mouse, Calbiochem cp06) primary antibodies were diluted 1:200 and 1:500 respectively in TBS plus 1% normal donkey serum and 0.1% Triton X-100 ...
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bioRxiv - Microbiology 2021Quote: ... Cells were split roughly 1:4 and supplemented with 1 mM Rock-inhibitor (Y27632; Calbiochem). Cells were fed with new media daily.
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bioRxiv - Neuroscience 2020Quote: ... collected on a microscope slide and coverslips were mounted with Mowiol 4-88 (MilliporeSigma Calbiochem). Primary antibodies were the following ...
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bioRxiv - Developmental Biology 2022Quote: ... the slides were washed with PBS and mounted with water based Mowiol 4-88 (Calbiochem) mounting media ...
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bioRxiv - Genomics 2023Quote: ... and immunoprecipitations were carried out by adding 4 μg of the appropriate antibodies (MPO, Calbiochem) to
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bioRxiv - Biochemistry 2023Quote: ... The coverslips were then mounted in Mowiol 4-88 solution (Calbiochem/EMD Millipore, Billerica, MA) and fluorescence analysis was performed using a Plan-Apochromat 63×/1.4 objective (Zeiss ...
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bioRxiv - Genomics 2024Quote: ... Sections were counterstained with 0.25 µg/mL DAPI and mounted in Mowiol 4-88 (Calbiochem). Imaging was performed using the Nikon SMZ18 stereomicroscope and Olympus FluoView FV3000 confocal microscope ...
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bioRxiv - Biochemistry 2022Quote: ... ScDIP2-GFP knock-in cells were resuspended in a cell resuspension buffer (50 mM Tris/HCl, pH 7.6, 0.6 M sorbitol, 1mM EDTA) containing protease inhibitor cocktail (Calbiochem) and 1mM PMSF (Sigma) ...
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bioRxiv - Neuroscience 2020Quote: ... Total mouse hippocampus from the left hemisphere was homogenised using a mechanical homogeniser and disposable pestles (Anachem) in 100μl TBS (50mM Tris-HCl pH 8.0) containing protease and phosphatase inhibitors (Calbiochem). The homogenate was then centrifuged at 4 °C for 30 mins at 16,000 × g ...
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bioRxiv - Neuroscience 2019Quote: ... diluted in KPBS containing 0.3 % Triton X-100 and incubated overnight at RT in cFos rabbit polyclonal antiserum (1:20,000; Calbiochem) diluted in KPBS containing 1 % NGS and 0.3 % Triton X-100 ...
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bioRxiv - Biochemistry 2021Quote: ... Cell pellets were sonicated in 20 mM HEPES buffer pH 7.5 containing 0.25 M sucrose and 1:100 protease inhibitor cocktail set V (Calbiochem) and centrifuged at 2,000 × g for 10 min to pellet nuclear fraction ...
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bioRxiv - Neuroscience 2023Quote: ... we used a pipette solution containing 50-100 ng/ml amphotericin B as permeabilizing agent (Calbiochem, Madrid, Spain) and a pipette-filling solution containing (in mM) ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... collected pRPE cells were suspended in a hypotonic buffer containing protease inhibitor cocktail (MilliporeSigma™ Calbiochem™, 53913110VL). The pRPE cells underwent disruption with a Dounce tissue homogenizer ...
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bioRxiv - Microbiology 2020Quote: ... mouse monoclonal IgG2a anti-SV40-LTag cross-reacting with BKPyV LTag (PAb416, Calbiochem), mouse monoclonal IgG1 anti-BKPyV Vp1 (MAB3204-M19 ...
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bioRxiv - Neuroscience 2019Quote: ... the slices were treated with pronase (1 mg / 4-6 ml) (Calbiochem-MilliporeSigma, Burlington, MA, USA) in incubation medium I at 31°C for 30-50 min ...
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bioRxiv - Cancer Biology 2020Quote: ... Blots were incubated with following primary antibodies at 4 C overnight: TP53 (OP43, Calbiochem, 1:1000), RB1 (Ab-5 ...
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bioRxiv - Microbiology 2023Quote: ... The reaction mixture contains 8 μg total proteins and 4 μg nitrocefin (Calbiochem, San Diego, CA). β-Lactamase activity was determined by measuring the absorbance at 486 nm (OD486nm).
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bioRxiv - Cell Biology 2023Quote: ... in PBS for 5 min at RT and cells were mounted in Mowiol 4-88 (Calbiochem) solution (Wei and Seemann ...
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bioRxiv - Neuroscience 2023Quote: ... and incubated at 4°C for 24hrs with primary rabbit monoclonal anti-CGRP antibody (Calbiochem PC205) diluted 1:200 in 0.4% BSA ...
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bioRxiv - Genetics 2023Quote: ... Cells were passaged every 3-4 days using Accutase in presence of ROCKi (Y-27632, Calbiochem). VitC supplementation was performed as for mESCs.
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bioRxiv - Cancer Biology 2021Quote: Cell pellets were sonicated in ice-cold lysis buffer (containing 20 mM Tris-HCl, pH 7.4, 5 mM EDTA, 10 mM Na4P2O7 (Calbiochem), 100 mM NaF ...
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bioRxiv - Cancer Biology 2021Quote: Cell cultures in 10cm dishes were scrape-harvested in 500 μL lysis buffer containing 0.2% (v/v) protease inhibitors (Protease Inhibitor Cocktail set III, Calbiochem). Lysis buffer comprised ...
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bioRxiv - Immunology 2020Quote: ... Fixed cells were washed three times in PBS and permeabilized by incubating them in PBS containing 5% non-fat milk and 0.05% saponin (Calbiochem). Coverslips were placed in primary antibody for 1 hour then washed 3x in PBS and placed in secondary antibody ...
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bioRxiv - Neuroscience 2020Quote: ... A half cortex was weighed on a microscale and homogenised in 4 volumes of ice-cold Tris-buffered saline (TBS) (50mM Tris-HCl pH 8.0) containing a cocktail of protease and phosphatase inhibitors (Calbiochem) using a handheld mechanical homogeniser and disposable pestles (Anachem) ...
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bioRxiv - Neuroscience 2020Quote: ... in approximately 5 volumes of ice-cold Tris-buffered saline (TBS) (50 mM Tris-HCL, pH 8.0) containing a cocktail of protease and phosphatase inhibitors (Calbiochem). Homogenates were centrifuged at 16,000 x g at 4 °C for 30 min ...
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bioRxiv - Neuroscience 2022Quote: ... A half cortex was weighed on a microscale and homogenised in 4 volumes of ice-cold Tris-buffered saline (TBS) (50mM Tris-HCl pH 8.0) containing a cocktail of protease and phosphatase inhibitors (Calbiochem) using a handheld mechanical homogeniser and disposable pestles (Anachem) ...
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bioRxiv - Neuroscience 2022Quote: A half cortex was weighed on a microscale and homogenised in 3 volumes of ice-cold Tris-buffered saline (TBS) (50mM Tris-HCl pH 8.0) containing a cocktail of protease and phosphatase inhibitors (Calbiochem) using a handheld mechanical homogeniser and disposable pestles (Anachem ...
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bioRxiv - Biochemistry 2021Quote: ... A custom compound library containing over 5000 compounds assembled from commercial sources (Sigma, Selleck, Enzo, Tocris, Calbiochem, and Symansis) were distributed in 24 plates using an Echo 550 (Labcyte ...