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Citations for Calbiochem :
151 - 200 of 213 citations for Mouse Eukaryotic translation initiation factor 4E binding protein 3 EIF4EBP3 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... Plates were then washed to remove free protein prior to addition of nitrocefin (Calbiochem) for 1 hour at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... and at 48 hpi cells transduced with Wasabi-pIPFCW2 were selected with 3-4 mg/ml puromycin (Calbiochem, 540411) and sorted for mid-range expression of Wasabi.
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bioRxiv - Neuroscience 2022Quote: ... 1.33 mM Ca2+ and 1 mM of the cAMP phosphodiesterase inhibitor 3-isobutyl-1-methylxanthine (IBMX: Calbiochem, Damstard, Germany) were added to the synaptosomes for 15 min ...
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bioRxiv - Cell Biology 2023Quote: ... The antibodies (Tables 2 and 3) were diluted according to the tables in PBS containing 1% BSA (126575, Calbiochem).
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bioRxiv - Cell Biology 2020Quote: ... for 1 hr followed by 1:500 dilution of anti-mouse Alexa-594 (Calbiochem) for 45 min at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... Mouse sections were mounted on glass slides and coverslipped with Mowiol (Calbiochem, Cat. 475904). Images were captured using an Olympus BX41 microscope with an Olympus camera DP-70 (Olympus Denmark A/S).
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bioRxiv - Cell Biology 2024Quote: ... anti-USP14 and HSC70 anti-mouse IgM-HRP conjugate (Calbiochem, Cat# 401225,1:10,000 dilution). Image Studio software (Licor ...
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bioRxiv - Cell Biology 2020Quote: ... Wnt-surrogate55 (0.15 nM, U-Protein Expression) and Rho kinase inhibitor (10 μM, Calbiochem Y27632) was added to the media whenever cells were at a single-cell stage to help recovery ...
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bioRxiv - Cell Biology 2021Quote: ... The antibodies listed in Table 2 and 3 were diluted according to the tables in PBS containing 1% BSA (126575, Calbiochem).
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bioRxiv - Developmental Biology 2021Quote: ... transferred to a 15 ml centrifuge tube containing warm 3 ml 0.5 mg ml−1 collagenase type II (Calbiochem, 234155) solution and incubated for 30 min at 37°C shaker at 180 rpm ...
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bioRxiv - Cell Biology 2023Quote: ... the cells were fixed with cold methanol (10 min at −20°C) and blocked for 30 min with 3% (w/v) bovine serum albumin (Calbiochem) in 1 x PBS at room temperature ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were washed 3 times with PBS and fixed to a slide using 5 μL of Mowiol (4-88 Reagent, Calbiochem). Fluorescent images were acquired with a Leica Dmi8 microscope (Leica Microsystems).
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bioRxiv - Neuroscience 2024Quote: ... The NPCs were differentiated into neurons in the absence of bFGF for at least 3 weeks and treated with 10 μM ROCK inhibitor (Y-27632; Calbiochem) during the first 24 h.
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bioRxiv - Pathology 2024Quote: ... NETs formation was induced for 3 hours at 37°C and 5% CO2 by stimulation with 10 µM of PAF (Calbiochem) on primed neutrophils and 25 µM on non-primed ones ...
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bioRxiv - Cell Biology 2022Quote: ... Mouse pancreatic islets were lysed with radioimmunoprecipitation assay buffer containing protease and phosphatase inhibitors (Calbiochem), and insoluble material was removed by centrifugation ...
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bioRxiv - Microbiology 2023Quote: ... followed by probing with an anti-mouse antibody conjugated to HRP (Calbiochem, Cat. No. 401253) for one h and washed three times with 0.05% Tween20 in 1X PBS ...
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bioRxiv - Bioengineering 2020Quote: ... The light/heavy peptide mixture was incubated with 60 μL protein G agarose bead slurry (Calbiochem) conjugated to an antibody cocktail containing 12 μg 4G10 (Millipore) ...
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bioRxiv - Microbiology 2023Quote: ... The reaction mixture contains 8 μg total proteins and 4 μg nitrocefin (Calbiochem, San Diego, CA). β-Lactamase activity was determined by measuring the absorbance at 486 nm (OD486nm).
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bioRxiv - Cell Biology 2019Quote: ... (N-{(2S,4R)-4-(Biphenyl-2-ylmethyl-isobutyl-amino)-1-[2-(2,4-difluorobenzoyl)-benzoyl]-pyrrolidin-2-ylmethyl}8-3-(4-(2,4-dioxothiazoldin-5-ylidenemethyl)-phenyl]acrylamide (cPLA2 inhibitor, cPLA2 Inh, Calbiochem, EMD Biosciences), was used concomitantly with priming at 5 μM and MK-886 (FLAP Inh ...
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bioRxiv - Microbiology 2022Quote: ... the preformed spheroids were transferred into the fibrin gel consisting of plasminogen-free human fibrinogen (final concentration 3 mg/ml; Calbiochem, USA) and human thrombin (final concentration 2 U/ml ...
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bioRxiv - Cell Biology 2024Quote: ... Membranes were incubated overnight at 4°C,with mouse anti-PGC-1α (Calbiochem, KP9803, 1:1000), mouse anti-PINK1 (Santa Cruz Biotechnology ...
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bioRxiv - Cancer Biology 2020Quote: Whole cell protein lysates were prepared in RIPA buffer supplemented with Phosphatase Inhibitor Cocktail Set-II (Calbiochem) and protease inhibitor (VWR ...
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bioRxiv - Neuroscience 2023Quote: ... after which the gels was washed with water and all protein was stained using RAPIDstain (Calbiochem, 553215) for 1 hour ...
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bioRxiv - Cancer Biology 2020Quote: ... The 2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one (PD98059, MEK inhibitor) and STAT3 inhibitor VI were from Calbiochem (Darmstadt, Germany). The EGF was from Millipore (Billerica ...
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bioRxiv - Neuroscience 2021Quote: ... they were incubated for 2 h in secondary antibody (anti-mouse fluorescein conjugated, Calbiochem cat n. 401234) 1:100 in BSA 1% + sheep serum 2% in PBT ...
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bioRxiv - Neuroscience 2022Quote: ... the retinas were treated with the primary antibodies (1000x + 1000x mouse SMI31 + SMI32 = SMI312, NE1022 / NE1023 - Calbiochem; 1000x rabbit Caspase3 ...
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bioRxiv - Molecular Biology 2023Quote: ... The lower half of the membrane was probed with mouse anti-GAPDH (Calbiochem clone 6C5 CB1001-500UG) at 1:10,000 while the upper half was probed with mouse anti-FLAG (Sigma-Aldrich/Millipore F1804-1MG ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: Brain tissue was homogenized in RIPA buffer supplemented with protease and protein phosphatase inhibitors (Calbiochem, La Jolla, CA) and subjected to SDS-PAGE followed by transferring to PVDF membranes (Millipore ...
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bioRxiv - Physiology 2023Quote: Total protein extracts were obtained from microalgal cells in exponential phase of growth (e.g. 4 days) in 50 mM Tris buffer pH 8.0 supplemented with protein inhibitor cocktail (539131, Calbiochem). Free-living and symbiotic microalgae were disrupted by bead beating with a Precellys device (Bertin Technologies ...
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bioRxiv - Biophysics 2022Quote: The activity of β-galactosidase was measured using a spectrophotometric assay in which o-Nitrophenyl-β-D-Galactopyranoside (ONPG, CAS Nr: 369-07-3 Calbiochem Sigma-Aldrich) was used as a substrate ...
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bioRxiv - Molecular Biology 2019Quote: The ProteoExtract® Subcellular Proteome Extraction Kit (Calbiochem) was used to generate lysates from the cytosolic ...
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bioRxiv - Cell Biology 2023Quote: ... mouse organs were homogenized in 2% SDS lysis buffer containing protease inhibitor mixture III (# 535140, Calbiochem, Billerica, MA) and phosphatase inhibitors (#P2850 ...
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bioRxiv - Neuroscience 2020Quote: ... (Cat. N° 487910) and 3-amino,4-aminomethyl-2’,7’-difluorofluorescein (DAF-FM) (Cat. N° 251515) were obtained from Calbiochem (San Diego, CA, USA). EZ-link HPDP-Biotin (Cat ...
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bioRxiv - Molecular Biology 2020Quote: ... or with polyclonal anti-Sir3 antibodies and immune complexes were collected with Protein G/A beads (Calbiochem, EMD Biosciences). Immunoprecipitated and input DNA were purified using Chelex 100 (Bio-Rad ...
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bioRxiv - Immunology 2021Quote: ... under the CD11c/Itgax promoter upon intraperitoneal (i.p.) injection of 4 ng/g of mouse body weight of diphtheria toxin (DT, Calbiochem) 12 hrs prior to the targeted CD11c+ cell depletion time as indicated in the relevant figure legends ...
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bioRxiv - Plant Biology 2021Quote: ... The chromatin complexes were immunoprecipitated with anti-GFP antibody (ab290) and an anti-myc mouse antibody (mAb 9E10, Calbiochem) with a concentration of 2 μg / sample and incubated in ChIP dilution buffer with 20 μL of Dynabeads Protein A (Thermofisher ...
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bioRxiv - Immunology 2021Quote: CCR2+ and CD11c+ cells were depleted from diphtheria toxin receptor (DTR)-expressing mice upon intraperitoneal (i.p.) injection of 10 ng/g of mouse body weight of diphtheria toxin (DT, Calbiochem) 12 hrs prior to Lm challenge infection.
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bioRxiv - Microbiology 2019Quote: ... Lysates were cleared by centrifugation and precipitated with anti-HA antibody (clone HA-7; Bioshop) using agarose protein G beads (Calbiochem) as previously described25 ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins were extracted using an 8M urea lysis buffer (8M urea, 50 mM Tris-HCl, 75 mM NaCl, 1x Calbiochem protease inhibitor ...
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bioRxiv - Plant Biology 2019Quote: ... After 30 min incubation on a rotator at 4°C the protein samples were centrifuged at 18,000xg for 30 min to remove plant debris and the protein extract was filtered through Miracloth filter paper (Calbiochem Co.). Next ...
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bioRxiv - Biochemistry 2022Quote: ... lysed in M-PER mammalian protein extraction reagent (Thermo-Fisher) with 1/1000 of the EDTA-free protease inhibitor cocktail Set III (Calbiochem), and centrifuged for 30 min at 15,000 × g at 4°C ...
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bioRxiv - Cell Biology 2019Quote: ... and incubated overnight at 4 °C with mouse monoclonal antibodies against type I collagen (20 μg/mL, CP-17, Calbiochem), type II collagen (0.4 μg/mL ...
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bioRxiv - Microbiology 2020Quote: ... Samples were incubated at 30°C for 1 h prior to SDS-PAGE and Western blotting with a mouse mono- and polyubiquitinated conjugate (Ubiquigent) or rabbit CHIP antibody (Calbiochem) with HRP-conjugated anti-mouse (GE Healthcare ...
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bioRxiv - Cell Biology 2021Quote: Commercially available antibodies against the following proteins were used for Western blotting in the indicated dilutions: BRCA2 (OP95, Calbiochem, 1:1000), RAD51 (70-001 ...
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bioRxiv - Genomics 2019Quote: ... and SV40 large T antigen of the BK virus (Affinity purified and agarose conjugated IgG2A mouse monoclonal antibody recognizing the 94kDa SV40 large T antigen; PAb416, Cat. No. DPO2, Calbiochem, USA) respectively.
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bioRxiv - Genetics 2023Quote: ... Raw values were normalized by geometric mean and used for subsequent housekeeping normalization (α-tubulin values; 1:10,000 mouse anti-tubulin; Calbiochem CP06) from the same blot.
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bioRxiv - Neuroscience 2021Quote: Calcineurin activity was assessed using Calcineurin activity assay kit (#20700, Calbiochem). Hippocampus tissue of 8 weeks old animals was homogenised in the lysis buffer (25 mM Tris-HCl pH 7.5 ...
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bioRxiv - Neuroscience 2024Quote: ... TdT Colorimetric FragEL DNA Fragmentation Detection Kit (QIA33 TdT FragEL; Calbiochem) was used for TUNEL staining ...
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bioRxiv - Neuroscience 2020Quote: Mice were submitted to 24 h of fasting and then received ip injections with either saline (0.9% NaCl) or mouse recombinant leptin (5 mg/kg body weight, Calbiochem, Billerica, MA, USA) immediately before the dark cycle ...
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bioRxiv - Neuroscience 2020Quote: Mice were submitted to 12 h of fasting and then received ip injections with mouse leptin (5 mg/kg body weight, Calbiochem, Billerica, MA, USA). After 1 h ...