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Citations for Calbiochem :
101 - 150 of 158 citations for 4 Keto 13 cis retinoic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2024Quote: ... Sections were counterstained with 0.25 µg/mL DAPI and mounted in Mowiol 4-88 (Calbiochem). Imaging was performed using the Nikon SMZ18 stereomicroscope and Olympus FluoView FV3000 confocal microscope ...
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bioRxiv - Developmental Biology 2022Quote: ... the slides were washed with PBS and mounted with water based Mowiol 4-88 (Calbiochem) mounting media ...
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bioRxiv - Biochemistry 2023Quote: ... The coverslips were then mounted in Mowiol 4-88 solution (Calbiochem/EMD Millipore, Billerica, MA) and fluorescence analysis was performed using a Plan-Apochromat 63×/1.4 objective (Zeiss ...
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bioRxiv - Cancer Biology 2020Quote: ... Blots were incubated with following primary antibodies at 4 C overnight: TP53 (OP43, Calbiochem, 1:1000), RB1 (Ab-5 ...
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bioRxiv - Cell Biology 2023Quote: ... in PBS for 5 min at RT and cells were mounted in Mowiol 4-88 (Calbiochem) solution (Wei and Seemann ...
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bioRxiv - Microbiology 2023Quote: ... The reaction mixture contains 8 μg total proteins and 4 μg nitrocefin (Calbiochem, San Diego, CA). β-Lactamase activity was determined by measuring the absorbance at 486 nm (OD486nm).
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bioRxiv - Genetics 2023Quote: ... Cells were passaged every 3-4 days using Accutase in presence of ROCKi (Y-27632, Calbiochem). VitC supplementation was performed as for mESCs.
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bioRxiv - Neuroscience 2023Quote: ... and incubated at 4°C for 24hrs with primary rabbit monoclonal anti-CGRP antibody (Calbiochem PC205) diluted 1:200 in 0.4% BSA ...
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bioRxiv - Cell Biology 2024Quote: ... Membranes were incubated overnight at 4°C,with mouse anti-PGC-1α (Calbiochem, KP9803, 1:1000), mouse anti-PINK1 (Santa Cruz Biotechnology ...
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bioRxiv - Neuroscience 2023Quote: Cortical neurons were incubated with 4 μM fura-2 acetoxymethyl ester (AM, Calbiochem, CAS 108964-32-5) 40 min at 37°C ...
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bioRxiv - Molecular Biology 2024Quote: ... and rotated at 4°C for 2 h with 2.5 µg of mouse anti-Myc antibody (Calbiochem). The beads were washed twice with wash buffer containing 300 mM NaCl and once with lysis buffer ...
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bioRxiv - Biophysics 2022Quote: ... were mixed in 70 μL of assembly buffer (50 mM HEPES-KOH, pH 7.5, 300 mM KCl, 5 mM MgCl2, 2 mM TCEP, 4% glycerol, 2 mM ATPγS [Calbiochem]) for 5 min at 25 °C ...
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bioRxiv - Neuroscience 2020Quote: ... sections were incubated overnight at 4°C in blocking solution with anti-TH (1:2,500; Chemicon) and anti-BiP (1:1,000; Calbiochem) antibodies and detected using secondary alexa-488 anti-rabbit and alexa-564 anti-mouse antibodies ...
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bioRxiv - Physiology 2023Quote: Total protein extracts were obtained from microalgal cells in exponential phase of growth (e.g. 4 days) in 50 mM Tris buffer pH 8.0 supplemented with protein inhibitor cocktail (539131, Calbiochem). Free-living and symbiotic microalgae were disrupted by bead beating with a Precellys device (Bertin Technologies ...
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bioRxiv - Immunology 2021Quote: ... under the CD11c/Itgax promoter upon intraperitoneal (i.p.) injection of 4 ng/g of mouse body weight of diphtheria toxin (DT, Calbiochem) 12 hrs prior to the targeted CD11c+ cell depletion time as indicated in the relevant figure legends ...
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bioRxiv - Physiology 2021Quote: ... cells were treated with poly(I:C) (1.5 μg/mL for 4 hrs) and a combination of proteasome inhibitor (Bortezomib, 2 μM, Sigma/Calbiochem 5043140001) and prolyl endopeptidase inhibitors (Pramiracetam 10 uM ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were rinsed with cold PBS1X and then lysed in Triton-X100 buffer at 4°C (50mM HEPES pH 7.4, 150mM NaCl, 1 % Triton X-100, protease inhibitors (Calbiochem) and phosphatase inhibitor cocktails II and IV (Calbiochem)) ...
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bioRxiv - Biochemistry 2021Quote: ... cells were trypsinized and seeded for selection in complete media supplemented with 4 μg/mL puromycin (Calbiochem, Merck Millipore). All cell lines were passaged 3 times before use and kept in culture supplemented with 2 μg/mL puromycin and/or stored in liquid nitrogen.
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bioRxiv - Plant Biology 2020Quote: ... Homogenate was centrifuged at 10,000 x g at 4°C for 10 minutes and filtered through two layers of Miracloth (Calbiochem) before centrifugation at 40,000 x g for one hour at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... 50 μg of the total RNA was incubated for 1 h at 4 °C with 10 μl of the anti-2,2,7-trimethylguanosine-antibody (Calbiochem Milipore) coupled to sepharose beads (Fig ...
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bioRxiv - Cell Biology 2022Quote: ... and at 48 hpi cells transduced with Wasabi-pIPFCW2 were selected with 3-4 mg/ml puromycin (Calbiochem, 540411) and sorted for mid-range expression of Wasabi.
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bioRxiv - Developmental Biology 2020Quote: ... allowed to dry for 30 min at 37 °C and mounted in MOWIOL 4-88 Reagent (Calbiochem, Darmstadt, Germany).
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bioRxiv - Cell Biology 2020Quote: ... and the phenylacetamide compound (S)-2-(4-chlorophenyl)-3,3-dimethyl-N-(5-phenylthiazol-2-yl) butanamide (Cmp58) was obtained from Calbiochem-Merck Millipore (Billerica ...
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bioRxiv - Cell Biology 2020Quote: ... The allosteric FFAR2 modulator Cmp58 ((S)-2-(4-chlorophenyl)-3,3-dimethyl-N-(5-phenylthiazol-2-yl)butanamide was obtained from Calbiochem-Merck Millipore (Billerica ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... and the phenylacetamide compound (S)-2-(4-chlorophenyl)-3,3-dimethyl-N-(5-phenylthiazol-2-yl)butanamide (PA;Cmp58 [30]) was obtained from Calbiochem-Merck Millipore (Billerica ...
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bioRxiv - Microbiology 2023Quote: ... Plates were then incubated at 37 °C for 2 h with different quantities (0.125 - 4 pmol) of biotinylated PfPP1c labeled with biotin-NHS (Calbiochem) in PBS-Tween 0.1% ...
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bioRxiv - Plant Biology 2023Quote: ... The homogenate was centrifuged at 4°C for 15 min at 10,000×g and the supernatant was filtered through Miracloth (Calbiochem) to remove plant debris ...
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bioRxiv - Microbiology 2022Quote: ... two reactions with increasing dosages of the PfCKII-α-specific inhibitors 4,5,6,7-tetramethylbromobenzotriazole and 4,5,6,7-tetramethylbromo-2-azabenzimidazole (TBB) (Merck/Calbiochem CAS 17374-26-4) were performed to check for possible suppression of phosphoryl group transfer ...
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bioRxiv - Cell Biology 2024Quote: ... Samples were incubated overnight at 4 °C with primary anti-RAD51 antibody (Calbiochem, PC130, 1:500 in 2% BSA), then washed five times ...
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bioRxiv - Neuroscience 2021Quote: ... allowed to dry for 30 min at 37 °C and mounted in MOWIOL® 4-88 (Calbiochem, Darmstadt, Ger-many).
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bioRxiv - Developmental Biology 2022Quote: ... Female Hbxip+/- mice (4-6 weeks) were induced to superovulate by intraperitoneal injection of 5 IU pregnant mare serum gonadotropin (PMSG, Calbiochem) and 48 hours later 5 IU human chorionic gonadotropin (hCG ...
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bioRxiv - Immunology 2021Quote: The caspase-1 activity was determined with the fluorometric substrate Z-YVAD 7-Amido-4-trifluoromethylcoumarin (Z-YVAD-AFC, caspase-1 substrate VI, Calbiochem), as described previously (Angosto et al. ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were washed twice with 3 ml PBS at 400 g for 5 minutes at 4°C and 500 uL PI staining buffer was added (1X PBS, 50 μg/mL PI (Calbiochem), 2μg/mL RNase A (Sigma ...
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bioRxiv - Cell Biology 2020Quote: ... the following inhibitors were used at the indicated concentrations for 4 h unless indicated differently in the experiments: RO-3306 at 10 μM (CDK1 inhibitor; Calbiochem), NU6140 at 10 μM (CDK2 inhibitor ...
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bioRxiv - Cell Biology 2020Quote: ... Samples were labeled with the indicated primary antibodies overnight at 4°C in the presence of 0.01% saponin (Calbiochem, 558255) and 1% BSA ...
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bioRxiv - Cell Biology 2022Quote: ... and AR-C118925 {5-[[5-(2,8-dimethyl-5H-dibenzo[a,d]cyclohepten-5-yl)-3,4-dihydro-2-oxo-4-thioxo-1(2H)-pyrimidinyl]methyl]-N-2H-tetrazol-5-yl-2-furancarboxamide} were obtained from Calbiochem-Merck Millipore (Billerica ...
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bioRxiv - Neuroscience 2021Quote: Pharmacological agents were used in the following concentrations and time courses: cycloheximide (Calbiochem, 0.2 μg/μl, 2, 4 or 8h), dexamethasone (Invivogen ...
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bioRxiv - Immunology 2022Quote: The caspase-1 activity was determined with the fluorometric substrate Z-YVAD 7-Amido-4-trifluoromethylcoumarin (Z-YVAD-AFC, caspase-1 substrate VI, Calbiochem), as described previously (Angosto et al. ...
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bioRxiv - Microbiology 2021Quote: ... parasites were fixed in 4% paraformaldehyde containing 0.0075% glutaraldehyde (Nacalai Tesque, Japan) and permeabilized with PBS containing 0.1% Triton-X100 (Calbiochem, CA, USA), then blocked with PBS containing 3% BSA ...
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bioRxiv - Biochemistry 2021Quote: ... resuspended in 4 ml ice-cold KHM buffer supplemented with 80 μg/ml high purity digitonin (Calbiochem, cat. number 300410) and incubated on ice for 5 min to permeabilise the plasma membrane ...
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bioRxiv - Neuroscience 2023Quote: ... O-GlcNAc transferase and O-GlcNAcase activities were blocked by 5 µM OSMI-4 (MedChem Express) and 5 µM Thiamet-G (Calbiochem) treatments ...
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bioRxiv - Biochemistry 2022Quote: ... Nuclei were stained with DAPI (1:1000 in PBS, AppliChem, Darmstadt, Germany) and coverslips were mounted on Mowiol 4-88 (Calbiochem, Merck Millipore ...
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bioRxiv - Immunology 2022Quote: Caspase-1 activity was determined with the fluorometric substrate Z-YVAD 7-Amido-4-trifluoromethylcoumarin (Z-YVAD-AFC, caspase-1 substrate VI, Calbiochem) as described previously [47,48] ...
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bioRxiv - Cancer Biology 2023Quote: ... The concentration of anti-E-A14-specific Ig in plasma was estimated in comparison with a standard curve generated with anti-Her2 monoclonal antibody (Ab-4; Calbiochem).
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bioRxiv - Cancer Biology 2022Quote: ... cells were washed 3 times with PBS and fixed to a slide using 5 μL of Mowiol (4-88 Reagent, Calbiochem). Fluorescent images were acquired with a Leica Dmi8 microscope (Leica Microsystems).
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bioRxiv - Neuroscience 2024Quote: ... was followed by primary antibody solution incubation overnight at 4 °C for detection of the following markers: monoclonal mouse: anti-APC CC-1 (1:50, Cat. No. OP80, Calbiochem); anti-MBP (1:500 ...
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bioRxiv - Microbiology 2024Quote: ... Viability was assessed after staining cells with 1 µg/mL propidium iodide (PI, stored at 4°C protected from light; Calbiochem). Cell number and percentage were obtained from gatings done on the Attune NxT software.
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bioRxiv - Neuroscience 2024Quote: ... Sections were incubated overnight with appropriate secondary antibodies (see Key resources table) and coverslipped with antifade mounting medium Mowiol (4-88 reagent, Calbiochem). For Mash1 staining ...
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bioRxiv - Neuroscience 2022Quote: ... the cells were then incubated overnight at 4°C with primary antibodies (rabbit anti-AUTS2, 1:200, Atlas Antibodies; rabbit anti-GRIA1, 1:10, Calbiochem; mouse anti-GRIA2 ...
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bioRxiv - Immunology 2023Quote: ... at a 4:1 molar ratio of monomer:streptavidin in the presence of a 1x protease inhibitor cocktail solution (Calbiochem, Millipore Sigma). Briefly ...