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Citations for Calbiochem :
51 - 100 of 102 citations for rno mir 542 5p RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2024Quote: ... 0.5 mM Na3VO4 and 1x Protease Inhibitor Cocktail Set 1 (PIC) (Calbiochem). Lysates were mixed by pipetting and rotated at 4°C for 20 min then subjected to centrifugation (12000 g for 10 min at 4°C) ...
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bioRxiv - Cell Biology 2020Quote: ... with a 1:1000 dilution of yeast protease inhibitor cocktail set IV (Calbiochem), the lysate was cleared by centrifugation at 4C at full speed on a microcentrifuge ...
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bioRxiv - Molecular Biology 2019Quote: ... 0.5% NP-40] mixed with protease inhibitor cocktail set III (EDTA-free, Calbiochem). Concentrations of protein samples were measured by BCA assay (Thermo Scientific ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were supplemented with 1X Protease Inhibitor Cocktail Set V EDTA-Free (Calbiochem), 10 mM MgCl2 ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were supplemented with 1X Protease Inhibitor Cocktail Set V EDTA-Free (Calbiochem), 10 mM MgCl2 ...
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bioRxiv - Cell Biology 2021Quote: ... 0.2 mM CaCl2) containing protease inhibitors (Protease Inhibitor Cocktail Set IV, Calbiochem, reference 539136), and centrifuged for 30 min at 160,000 x g at 4°C ...
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bioRxiv - Physiology 2021Quote: ... 0.1% sodium dodecyl sulfate) containing protease inhibitor cocktail (Cocktail set 1, Calbiochem, Bilerica, MA) and phosphatase inhibitors (25 mM NaF ...
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bioRxiv - Cell Biology 2022Quote: ... pH 6.8) enriched in protease inhibitor R cocktail set III (EDTA free; 539134; Calbiochem) and phosphatase inhibitors (cocktail 2 ...
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bioRxiv - Cancer Biology 2021Quote: ... 1% Triton −X-100 and 1× EDTA-free protease inhibitor cocktail (Calbiochem set III, 539134) in PBS ...
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bioRxiv - Molecular Biology 2020Quote: ... The tissues were homogenized in RIPA lysis buffer with protease inhibitor cocktail set lll (Calbiochem). After centrifugation ...
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bioRxiv - Cancer Biology 2019Quote: ... pH 7.4) containing protease inhibitor cocktail set I (Calbiochem-Novabiochem Corp., San Diego, CA, USA). The collected lysate proteins were then subjected to immunoblotting with LC3 (L7543 ...
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bioRxiv - Cell Biology 2019Quote: ... and resuspended them twice in PBS supplemented with Protease Inhibitor Cocktail Set III (Merck / Calbiochem, 539134) diluted 1:1000 ...
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bioRxiv - Cell Biology 2019Quote: ... and resuspended them twice in PBS supplemented with Protease Inhibitor Cocktail Set III (Merck / Calbiochem, 539134) diluted 1:1000 ...
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bioRxiv - Neuroscience 2020Quote: ... 5 μg/ml RNAse A pH 7.4 and protease inhibitor cocktail set III EDTA-free (Calbiochem) added of 1 mM PMSF ...
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bioRxiv - Cell Biology 2019Quote: ... 1 mM EDTA) containing a protease inhibitor mixture (1:1,000; Protease Inhibitor Cocktail set III; Calbiochem) for about 5 min and cut into small pieces ...
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bioRxiv - Immunology 2020Quote: ... 150 mM NaCl in the presence of Protease Inhibitor Cocktail Set III (Calbiochem®, #539-134) and 0.2 mg Na orthovanadate/ml for 5 min on ice ...
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bioRxiv - Immunology 2020Quote: ... 150 mM NaCl in the presence of Protease Inhibitor Cocktail Set III (Calbiochem®, #539-134) and 0.2 mg Na orthovanadate/ml for 5 min on ice ...
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bioRxiv - Developmental Biology 2023Quote: Cells were harvested in RIPA buffer with 1:100 protease inhibitor cocktail set I (Calbiochem, #539131) and phosphatase inhibitors (1:100 Na2Ov4 ...
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bioRxiv - Cancer Biology 2020Quote: Whole cell protein lysates were prepared in RIPA buffer supplemented with Phosphatase Inhibitor Cocktail Set-II (Calbiochem) and protease inhibitor (VWR ...
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bioRxiv - Immunology 2023Quote: ... 150 mM NaCl in the presence of Protease inhibitor Cocktail Set III (Calbiochem®, Merck, Darmstadt, Germany) and the phosphatase inhibitor sodium orthovanadate (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2020Quote: ... beads were incubated with astrocyte lysate buffer supplemented with protease inhibitors (Merck, Calbiochem set III, Cat. No. 539134) for one hour at 4°C on a rotating wheel ...
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bioRxiv - Cancer Biology 2022Quote: The cells were lysed in radioimmunoprecipitation assay buffer (RIPA buffer) supplemented with phosphatase inhibitor cocktail set-II (Calbiochem) and protease inhibitor (VWR) ...
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bioRxiv - Cell Biology 2020Quote: ... and the cells were washed with ice-cold PBS(-/-) with 1X Protease Inhibitor Cocktail Set 1 (Calbiochem 539131). The PBS was aspirated ...
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bioRxiv - Physiology 2021Quote: ... and phosphatase inhibitors (2 μM microcystin-LR, and 1× Phosphatase-Inhibitor Sets I and II (Calbiochem, Hayward, CA). We centrifuged homogenates at 4,000 g for 15 minutes to remove debris ...
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bioRxiv - Biochemistry 2021Quote: ... Cell pellets were sonicated in 20 mM HEPES buffer pH 7.5 containing 0.25 M sucrose and 1:100 protease inhibitor cocktail set V (Calbiochem) and centrifuged at 2,000 × g for 10 min to pellet nuclear fraction ...
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bioRxiv - Pathology 2023Quote: ... 2% DDM) supplemented with 1:1000 (v/v) Protease Inhibitor Cocktail Set III (Calbiochem, La Jolla, CA, USA). Rat anti-HA (3F10 ...
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bioRxiv - Cancer Biology 2021Quote: Cell cultures in 10cm dishes were scrape-harvested in 500 μL lysis buffer containing 0.2% (v/v) protease inhibitors (Protease Inhibitor Cocktail set III, Calbiochem). Lysis buffer comprised ...
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bioRxiv - Biophysics 2022Quote: ... Cell pellets were lysed with an Emulsiflex (Avastin) in the presence of protease inhibitor cocktail Mixture Set V (Calbiochem) and DNAse (Millipore Sigma) ...
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bioRxiv - Molecular Biology 2022Quote: ... pellets were resuspended in 800 μl of ice-cold wash buffer (50 mM NaH2PO4, 300 mM NaCl, 0.05% Tween, 1:200 diluted protease inhibitor cocktail set III (Calbiochem)) supplemented with 0.1 U/μL of recombinant RNase inhibitor (wash buffer-RIn ...
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bioRxiv - Neuroscience 2023Quote: ... Heads were snap-frozen in liquid nitrogen then homogenized using a mechanical homogenizer in 100 μL TBS (50mM Tris-HCl, pH 8.0) containing 1:200 protease inhibitor cocktail set III (Calbiochem). Homogenates were centrifuged at 16,000 g at 4°C for 30 min ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... and 0.2% soybean phosphatidylcholine with 100 μl of protease inhibitor cocktail (Protease Inhibitor Cocktail Set III, EDTA-Free, Calbiochem). After stirring for 1 hour at 4°C ...
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bioRxiv - Evolutionary Biology 2021Quote: ... blocked (200 μL, PBS 1% BSA wt/vol, Calbiochem #12659-100GM, 3 h, RT) and washed (3x ...
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bioRxiv - Immunology 2020Quote: ... blocked (200 µL, PBS 1% BSA wt/vol, Calbiochem #12659-100GM, 3 h, RT) and washed (3x ...
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bioRxiv - Cell Biology 2021Quote: ... the cell pellet was lysed by sonication in lysis buffer (2mM Tris–HCl pH 7.4, 0.5M NaCl, 20mM imidazole, 10% glycerol, Protease Inhibitor Cocktail Set III Calbiochem, 1:10,000) and centrifuged (10,000g ...
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bioRxiv - Cell Biology 2022Quote: ... Forty-eight hours post transfection cells were removed and incubated in 250 μL/well of HEK Lysis Buffer containing protease Inhibitor Cocktail Set V (Calbiochem) before being centrifuged at 13 000 rpm for 10 min ...
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bioRxiv - Biochemistry 2022Quote: ... lysed in M-PER mammalian protein extraction reagent (Thermo-Fisher) with 1/1000 of the EDTA-free protease inhibitor cocktail Set III (Calbiochem), and centrifuged for 30 min at 15,000 × g at 4°C ...
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bioRxiv - Immunology 2020Quote: ... Cell lysates were collected in RIPA buffer plus inhibitors (protease: complete mini Roche; 11836153001, and phosphatases: cocktail set II Calbiochem; 524625) and then incubated with the antibody-bead mix overnight at 4°C on a shaker ...
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bioRxiv - Bioengineering 2022Quote: ... All the above stages were performed in cold phosphate-buffered saline (PBS) with 1 × Protease Inhibitor cocktail set I (Calbiochem 539,131). Eyes that were initially fixed in 10% NBF or 2.5% PFA were dissected to create eyecups ...
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bioRxiv - Cell Biology 2020Quote: ... placed on ice and lysed in 1 ml of lysis buffer (1 × TBS + freshly added protease inhibitor cocktail set I (Calbiochem, Darmstadt, Germany)) + 10 μl of 100 mM PMSF ...
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bioRxiv - Cell Biology 2020Quote: ... and all cell types were lysed with Pierce™ RIPA Buffer (ThermoScientific 89900) supplemented with 1X Phosphatase Inhibitor Cocktail Set 2 (Calbiochem 5246251) and 1X Protease Inhibitor Cocktail Set 1 (Calbiochem 539131) ...
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Myosin VI regulates ciliogenesis by promoting the turnover of the centrosomal/satellite protein OFD1bioRxiv - Cell Biology 2021Quote: ... pH 7.5, 250 mM NaCl, 1 mM EDTA, 5% Glycerol, 0.1% NP40, Protease Inhibitor Cocktail set III, Calbiochem, and 0.1 mM PMSF). Sonicated lysates were cleared by centrifugation at 16,000 rpm for 30 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... in PBS for 5 min at RT and cells were mounted in Mowiol 4-88 (Calbiochem) solution (Wei and Seemann ...
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bioRxiv - Cell Biology 2021Quote: ... pre-equilibrated with running buffer (PBS, 20mM imidazole, 10% glycerol, protease Inhibitor Cocktail Set III Calbiochem, Ref. 539134-1 at 1:10,000 dilution). The column was washed with five column volumes (CV ...
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bioRxiv - Microbiology 2021Quote: ... to OD600 1 were incubated with 750 μl of lysis buffer (100 mM EDTA [pH 8.0], 1 mM MgCl2, 5 μg/ml lysostaphin, 10 μg/ml DNase, proteinase inhibitor [cocktail set III from Calbiochem] diluted 1:100 in PBS) for 30 min at 37°C and homogenized three times with 500 μl zirconia-silica beads (0.1 mm diameter from Carl-Roth ...
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bioRxiv - Neuroscience 2019Quote: ... diluted in KPBS containing 0.3 % Triton X-100 and incubated overnight at RT in cFos rabbit polyclonal antiserum (1:20,000; Calbiochem) diluted in KPBS containing 1 % NGS and 0.3 % Triton X-100 ...
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bioRxiv - Neuroscience 2021Quote: ... the brain was dissected and homogenized in RIPA extraction buffer (10mM HEPES/NaOH pH 7.4, 150mM NaCl, 1% Triton X-100, 1% SDS) with protease inhibitors (Calbiochem Protease Inhibitor Set V EDTA-free). Western blot analysis was performed with Bio-Rad wet transfer system and PVDF membrane (Millipore) ...
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bioRxiv - Neuroscience 2020Quote: ... Bacteria were resuspended in (50mM HEPES, 250mM NaCl, 10% glycerol plus 1% Triton X-100, supplemented with protease inhibitors (Merck, Calbiochem set III, Cat. No. 539134) and subsequently sonicated ...
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bioRxiv - Neuroscience 2020Quote: ... Cell lysis of all samples was performed after two washes with ice-cold PBS by incubating cells for 20 min in ice-cold 1% Triton X100 in PBS with protease inhibitor cocktails (Merck, Calbiochem set III, Cat. No. 539134), subsequent thorough scraping and sonication.
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bioRxiv - Cell Biology 2022Quote: ... cells were washed with ice-cold PBS and lysed with ice-cold IP buffer (10 mM HEPES pH 7.4; 150 mM NaCl, 1% Triton X-100, plus protease inhibitor cocktail Set I-Calbiochem 1:100 (Cat# 539131, Merck Millipore), 1 mM Na3VO4 and 1mM NaF) ...
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bioRxiv - Cell Biology 2022Quote: ... or kinase domain ERK3 in kinase buffer (250 mM HEPES pH 7.5, 500 mM NaCl, 25 mM MgCl2, supplemented with protease inhibitor cocktail Set I-Calbiochem 1:100 (Cat# 539131, Merck Millipore) and phosphatase inhibitors ...