Labshake search
Citations for Calbiochem :
51 - 100 of 104 citations for Plasmodium falciparum HRP II Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2020Quote: ... Rac1 inhibitor (NSC23766) and Arp-2 complex inhibitor I (CK-666) & II (CK-869) purchased from Calbiochem were used for blocking actin assembly (33).
-
bioRxiv - Microbiology 2020Quote: ... the pro-protein convertase inhibitor Decanoyl-RVKR-CMK (Calbiochem) was applied to cells at a concentration of 5 μM ...
-
bioRxiv - Biochemistry 2022Quote: ... The protein extracts were first filtered through Miracloth (CalBiochem) to remove insoluble plant debris and subsequently centrifuged at 5,000 x g for 5 min at 4°C to remove cellular debris and organelles ...
-
bioRxiv - Immunology 2021Quote: ... then precipitated with the ProteoExtract Protein Precipitation Kit (Calbiochem). Equal amounts of protein for each set of samples (e.g ...
-
bioRxiv - Immunology 2022Quote: ... prior to protein precipitation (Calbiochem Proteo-Extract precipitation kit). Precipitants were resuspended in 1% SDS and quantified by BCA assay for normalization ...
-
bioRxiv - Biochemistry 2024Quote: ... Proteins were precipitated out using ProteoExtract precipitation kit (Calbiochem) and dissolved in 1X Laemmli buffer ...
-
bioRxiv - Microbiology 2020Quote: ... and overlaid with standard cell culture media plus 0.37% (w/vol) sodium bicarbonate and 0.8% (w/vol) Aquacide II (Millipore Calbiochem). After 7 days incubation at 37 °C ...
-
bioRxiv - Molecular Biology 2022Quote: ... Cells were infected for 1 h and an overlay of 1% carboxymethylcellulose (Aquacide II, Calbiochem, San Diego, CA) prepared in RPMI was added ...
-
bioRxiv - Cancer Biology 2022Quote: The cells were lysed in radioimmunoprecipitation assay buffer (RIPA buffer) supplemented with phosphatase inhibitor cocktail set-II (Calbiochem) and protease inhibitor (VWR) ...
-
bioRxiv - Physiology 2021Quote: ... and phosphatase inhibitors (2 μM microcystin-LR, and 1× Phosphatase-Inhibitor Sets I and II (Calbiochem, Hayward, CA). We centrifuged homogenates at 4,000 g for 15 minutes to remove debris ...
-
bioRxiv - Microbiology 2019Quote: ... 20 µL protein A agarose suspension (Calbiochem/Millipore #IP02-1.5ML) was added to each and samples were rocked at 4°C for 2 hours ...
-
bioRxiv - Cell Biology 2021Quote: ... and proteins were immunoprecipitated on BRCA2 antibody-conjugated (OP95, Calbiochem) Rec-protein G Sepharose 4B beads (Invitrogen ...
-
bioRxiv - Cell Biology 2019Quote: ... cells were lysed at 1 × 109 cells/ml in ice-cold lysis buffer (0.1 µM TLCK, 1 µg/ml Leupeptin, Phosphatase Inhibitor Cocktail II (Calbiochem),1 mM PMSF ...
-
bioRxiv - Molecular Biology 2022Quote: ... The cell pellet was lysed at 0.5 × 109 cells/ml in ice-cold lysis buffer (0.1 mM TLCK, 1 μg/ml Leupeptin, 1x Phosphatase Inhibitor Cocktail II tablet (Calbiochem), 1 mM PMSF ...
-
bioRxiv - Developmental Biology 2022Quote: ... Tissues were homogenized in cold lysis buffer (0.1% NP-40, 50mM Tris-HCl [pH 7.2], 250mM NaCl, 2mM EDTA, phosphatase inhibitor mixture I and II [Calbiochem] and one tablet of Minicomplete [Roche] per 10ml ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... Suc-Ala-Ala-Pro-Phe-AMC (Chymotrypsin substrate II) and Z-Gly-Gly-Arg-AMC.HCl (Urokinase substrate III) were from Calbiochem, and MCA-Lys-Pro-Leu-Gly-Leu-Dpa(DNP)-Ala-Arg-NH2 (MMP substrate FS-6 ...
-
bioRxiv - Cancer Biology 2021Quote: ... and the epithelial progenitors were isolated by mincing and enzymatic digestion using collagenase type II (0.5mg/mL) (Calbiochem, # 234155) (2.5 h ...
-
bioRxiv - Developmental Biology 2021Quote: ... transferred to a 15 ml centrifuge tube containing warm 3 ml 0.5 mg ml−1 collagenase type II (Calbiochem, 234155) solution and incubated for 30 min at 37°C shaker at 180 rpm ...
-
bioRxiv - Physiology 2021Quote: ... or 0.2 mL (for lung) HEPES-buffered saline plus Phosphatase Inhibitor Cocktail Set II and Protease Inhibitor Cocktail Set III (Calbiochem). Protein was measured using a Pierce™ BCA protein assay kit (Thermo Fisher ...
-
bioRxiv - Biochemistry 2020Quote: ... Lambda protein phosphatase and alkaline phosphatase were from Calbiochem (#539514-20KV) and Sigma-Aldrich (#P6774-2KU) ...
-
bioRxiv - Cancer Biology 2022Quote: ... Purified proteins were dialyzed against 20 mm Tris/HCl (MERCK, Calbiochem, catalogue No ...
-
bioRxiv - Neuroscience 2024Quote: ... and 50 µM Rho-associated protein kinase inhibitor (ROCKi; Calbiochem, USA). This media was changed every other day for 6 days ...
-
bioRxiv - Immunology 2020Quote: ... Cell lysates were collected in RIPA buffer plus inhibitors (protease: complete mini Roche; 11836153001, and phosphatases: cocktail set II Calbiochem; 524625) and then incubated with the antibody-bead mix overnight at 4°C on a shaker ...
-
bioRxiv - Cancer Biology 2022Quote: ... Protein A/G plus agarose suspension solution was obtained from Calbiochem (MERCK) and Dimethyl Sulfoxide (DMSO ...
-
bioRxiv - Cancer Biology 2021Quote: ... cells were collected using 75μl Lysis Buffer (Cell Signaling Technology #9803, Phosphatase Inhibitor Cocktail Set II #524625 and Protease Inhibitor Cocktail Set III #539134 both Calbiochem). Upon cell lysis using a sonicator ...
-
bioRxiv - Genetics 2021Quote: ... cells were treated with 50 μM of the protein synthesis inhibitor cycloheximide (Calbiochem), or ethanol as vehicle control during 4 h or 8 h ...
-
bioRxiv - Bioengineering 2021Quote: ... pH 7.4] and incubated with 60 μL protein G agarose bead slurry (Calbiochem) conjugated to an antibody cocktail containing 24 μg 4G10 (Millipore ...
-
bioRxiv - Cell Biology 2021Quote: 20 mg of mitochondria were lysed with digitonin (Calbiochem; 6.6 g/g protein) for 15 min on ice with occasional vortexing and cleared from insoluble material for 20 min at 20,000 × g at 4°C ...
-
bioRxiv - Microbiology 2022Quote: ... 10 µM of indicated proteins were mixed with 100 µM nitrocefin (Calbiochem, Sigma) in a reaction buffer containing EstG storage buffer (50 mM HEPES-NaOH pH 7.2 ...
-
bioRxiv - Cell Biology 2022Quote: ... followed by the addition 40μl of Protein A or G–Sepharose beads (Calbiochem) and incubated for 4h at 4°C ...
-
bioRxiv - Developmental Biology 2022Quote: ... late third instar larval tissues were ex vivo cultured in 2 ml of WM1 medium in the presence or absence of a cocktail of Furin inhibitor I and II (50 µM final concentration each; Calbiochem; 344930 and 344931). Cultured discs were removed from a single pool of culture media after 0 ...
-
bioRxiv - Molecular Biology 2020Quote: ... Plates were then washed to remove free protein prior to addition of nitrocefin (Calbiochem) for 1 hour at room temperature ...
-
Noradrenergic alpha-2A receptor activation suppresses courtship vocalization in male Japanese quail.bioRxiv - Animal Behavior and Cognition 2021Quote: ... Correct placement of the cannula was confirmed 1 day after surgery by infusing 1 μg of human angiotensin-II (Calbiochem, San Diego, CA, USA) dissolved in 0.9% NaCl ...
-
bioRxiv - Cell Biology 2020Quote: ... Wnt-surrogate55 (0.15 nM, U-Protein Expression) and Rho kinase inhibitor (10 μM, Calbiochem Y27632) was added to the media whenever cells were at a single-cell stage to help recovery ...
-
bioRxiv - Cancer Biology 2022Quote: ... Immunodetection of RAS proteins was carried out with pan-RAS (Ab-3; Calbiochem; 1:1,000) antibodies ...
-
bioRxiv - Bioengineering 2020Quote: ... The light/heavy peptide mixture was incubated with 60 μL protein G agarose bead slurry (Calbiochem) conjugated to an antibody cocktail containing 12 μg 4G10 (Millipore) ...
-
bioRxiv - Biochemistry 2020Quote: ... all fractions were individually precipitated using the Proteoextract Protein Precipitation Kit (EMD Milipore, Calbiochem, 539180-1KIT). For each 600 µL fraction ...
-
bioRxiv - Microbiology 2023Quote: ... The reaction mixture contains 8 μg total proteins and 4 μg nitrocefin (Calbiochem, San Diego, CA). β-Lactamase activity was determined by measuring the absorbance at 486 nm (OD486nm).
-
bioRxiv - Neuroscience 2023Quote: ... after which the gels was washed with water and all protein was stained using RAPIDstain (Calbiochem, 553215) for 1 hour ...
-
bioRxiv - Genetics 2022Quote: ... an aliquot of the cushion supernatant and eluate were precipitated using the ProteoExtract protein precipitation kit (Calbiochem 539180) per manufacturer protocol and redissolved in 2X Laemmli buffer (Fisher 50-196-784) ...
-
bioRxiv - Animal Behavior and Cognition 2022Quote: Brain tissue was homogenized in RIPA buffer supplemented with protease and protein phosphatase inhibitors (Calbiochem, La Jolla, CA) and subjected to SDS-PAGE followed by transferring to PVDF membranes (Millipore ...
-
bioRxiv - Physiology 2023Quote: Total protein extracts were obtained from microalgal cells in exponential phase of growth (e.g. 4 days) in 50 mM Tris buffer pH 8.0 supplemented with protein inhibitor cocktail (539131, Calbiochem). Free-living and symbiotic microalgae were disrupted by bead beating with a Precellys device (Bertin Technologies ...
-
bioRxiv - Neuroscience 2019Quote: ... Fyn was IPed with 1µg Fyn antibody (mouse monoclonal antibody [mAb], sc-434, SantaCruz) and 10 µL Protein G plus-agarose (CalBiochem). Washed Protein G bead pellets were resuspended in 2X Laemmli buffer and subjected to Western blot analysis ...
-
bioRxiv - Molecular Biology 2020Quote: ... or with polyclonal anti-Sir3 antibodies and immune complexes were collected with Protein G/A beads (Calbiochem, EMD Biosciences). Immunoprecipitated and input DNA were purified using Chelex 100 (Bio-Rad ...
-
bioRxiv - Microbiology 2019Quote: ... Lysates were cleared by centrifugation and precipitated with anti-HA antibody (clone HA-7; Bioshop) using agarose protein G beads (Calbiochem) as previously described25 ...
-
bioRxiv - Cell Biology 2022Quote: ... Proteins were extracted using an 8M urea lysis buffer (8M urea, 50 mM Tris-HCl, 75 mM NaCl, 1x Calbiochem protease inhibitor ...
-
bioRxiv - Plant Biology 2019Quote: ... After 30 min incubation on a rotator at 4°C the protein samples were centrifuged at 18,000xg for 30 min to remove plant debris and the protein extract was filtered through Miracloth filter paper (Calbiochem Co.). Next ...
-
bioRxiv - Molecular Biology 2021Quote: ... The expression of recombinant PGC-1α proteins was induced for 3 h at 37°C with 0.4 mM isopropyl-β-D-thiogalactoside (IPTG, Calbiochem) in Terrific Broth and purified by metal ion affinity chromatography (IMAC ...
-
bioRxiv - Biochemistry 2022Quote: ... lysed in M-PER mammalian protein extraction reagent (Thermo-Fisher) with 1/1000 of the EDTA-free protease inhibitor cocktail Set III (Calbiochem), and centrifuged for 30 min at 15,000 × g at 4°C ...
-
bioRxiv - Cell Biology 2021Quote: Commercially available antibodies against the following proteins were used for Western blotting in the indicated dilutions: BRCA2 (OP95, Calbiochem, 1:1000), RAD51 (70-001 ...