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Citations for Calbiochem :
51 - 75 of 75 citations for Dnase Free and Rnase Free Distilled Water since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... After 4 h the cells were starved in serum free medium overnight and then incubated for 1h with the ADAM17 inhibitor Batimastat (BB-94) at 10 μM (196440, Calbiochem), with the selective ADAM10 inhibitor GI254023X (GI ...
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bioRxiv - Microbiology 2022Quote: ... the preformed spheroids were transferred into the fibrin gel consisting of plasminogen-free human fibrinogen (final concentration 3 mg/ml; Calbiochem, USA) and human thrombin (final concentration 2 U/ml ...
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bioRxiv - Molecular Biology 2024Quote: ... cells were released from the arrest by transferring them to α-factor-free medium containing 150 μg/mL of Pronase (Calbiochem) and cultured for 30 min ...
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bioRxiv - Neuroscience 2024Quote: ... free-floating sections were incubated overnight at 4°C with a polyclonal rabbit anti-tyrosine hydroxylase (TH) antibody (Calbiochem, 657012-100μL) diluted 1:250 ...
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bioRxiv - Immunology 2020Quote: ... The filtrate was collected in 1.5ml microtube followed by addition of protease inhibitors (Protease Inhibitor Cocktail SetIII, EDTA-free; Calbiochem, San Diego, CA) to protect proteins from endogenous protease activities.
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bioRxiv - Neuroscience 2021Quote: ... the brain was dissected and homogenized in RIPA extraction buffer (10mM HEPES/NaOH pH 7.4, 150mM NaCl, 1% Triton X-100, 1% SDS) with protease inhibitors (Calbiochem Protease Inhibitor Set V EDTA-free). Western blot analysis was performed with Bio-Rad wet transfer system and PVDF membrane (Millipore) ...
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bioRxiv - Microbiology 2021Quote: ... and treated with DNase I (Calbiochem/MilliporeSigma ...
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bioRxiv - Developmental Biology 2023Quote: ... and 10 µg/ml DNAse I (Calbiochem, 260913) and filtered through a 40 µm cell strainer (Falcon ...
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bioRxiv - Molecular Biology 2020Quote: ... 0.4 ng/μl RNase A and protease inhibitor cocktails (Calbiochem)) and sonicated ...
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bioRxiv - Cell Biology 2022Quote: ... and DNase I (2,000 U/mL) (Calbiochem, Darmstadt, Germany). Tissue was incubated at 37°C for 16 min ...
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bioRxiv - Genetics 2020Quote: ... RNA was removed by adding 50 µg/ml of RNAse A (CalBiochem) in each tube which was incubated at room temperature for 1 h and then DNA was purified according the protocol described by [23] ...
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bioRxiv - Microbiology 2022Quote: ... and DNase I treated (Calbiochem; 20 µg/ml of supernatant) in the presence of 10 mM magnesium chloride (MgCl2 ...
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bioRxiv - Developmental Biology 2023Quote: ... Enzymatic reaction was blocked by adding PBS+10% FBS+1% DNAse (Calbiochem, 260913) and cell suspension was filtered and centrifuged at 1000 rpm for 5 min ...
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bioRxiv - Cell Biology 2021Quote: ... samples were washed with water and mounted on coverslips with Mowiol 4-88 (Calbiochem). Images were acquired using Nyquist criterion with a Leica TCS SPE confocal system with a 63x ACS APO/ NA 1.3 oil immersion lens and galvanometer driven stage insert and deconvolved using Huygens software.
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bioRxiv - Developmental Biology 2022Quote: ... the slides were washed with PBS and mounted with water based Mowiol 4-88 (Calbiochem) mounting media ...
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bioRxiv - Physiology 2020Quote: ... Kinase inhibiting RNase Attenuator 6 (KirA6; #5322810001) and IRE1 inhibitor III 4µ8C (#412512) were obtained from Calbiochem. IRE1 RNase activity inhibitor KirA8 (#HY-114368 ...
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bioRxiv - Neuroscience 2023Quote: ... after which the gels was washed with water and all protein was stained using RAPIDstain (Calbiochem, 553215) for 1 hour ...
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bioRxiv - Cell Biology 2024Quote: ... and 50 µg/ml cycloheximide (diluted from 10mg/ml stock prepared in water; Calbiochem, 23-97-64). As a control for either thapsigargin or bafilomycin- treatments ...
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bioRxiv - Molecular Biology 2021Quote: ... water samples from each source were collected and filtered through two layers of miracloth (Calbiochem, La Jolla, CA). One ml of the filtrate was spread aseptically onto YPS media and incubated in the dark at 24°C ...
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bioRxiv - Microbiology 2023Quote: ... Conidia were harvested in sterile water with 0.05 % (vol/vol) Tween20.The resulting suspension was filtered through two layers of gauze (Miracloth, Calbiochem). FITC-labelling of conidia was performed with 0.1 mg/ml FITC (Sigma ...
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bioRxiv - Molecular Biology 2020Quote: ... Coverslips were washed twice with PBS and once with water and mounted onto microscope slides using an antifade mounting medium (FluorSave, Calbiochem). All antibodies used in the present study are listed in Supplementary Table 2.
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bioRxiv - Biochemistry 2024Quote: ... 300 mM NaCl and 10 mM imidazole) and incubated with DNase I (10 mg/ml) and protease inhibitor cocktail (Calbiochem) at 4°C ...
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bioRxiv - Bioengineering 2021Quote: ... Protease Inhibitor Cocktail Set I and phosphate buffered saline (PBS) tablets (140mM NaCl, 10mM phosphate buffer, 3mM KCl reconstituted in 1L of deionized water) were from Calbiochem (San Diego, CA). Collagenase 2 and collagenase 4 were from Worthington Biochemical Corporation (Lakewood ...
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bioRxiv - Immunology 2021Quote: B cell staining was performed on frozen PBMCs after thawing in complete medium (RPMI 10% FBS, PS/G 1X) containing DNase I (Calbiochem, cod 260913) for 10 min at 37°C ...
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bioRxiv - Microbiology 2021Quote: ... to OD600 1 were incubated with 750 μl of lysis buffer (100 mM EDTA [pH 8.0], 1 mM MgCl2, 5 μg/ml lysostaphin, 10 μg/ml DNase, proteinase inhibitor [cocktail set III from Calbiochem] diluted 1:100 in PBS) for 30 min at 37°C and homogenized three times with 500 μl zirconia-silica beads (0.1 mm diameter from Carl-Roth ...