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Citations for Calbiochem :
51 - 100 of 687 citations for 7' BROMO 2' 3' DIHYDRO 1'H SPIRO CYCLOPROPANE 1 4' ISOQUINOLINE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... 3 and 6 hours with the inhibitors for ILK (1 μM CPD022, Calbiochem, #407331), FAK (5 μM FAK inhibitor 14 ...
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bioRxiv - Molecular Biology 2022Quote: ... The reaction was started by adding 1 nM to 4 μM calmodulin (Calbiochem) and 100 μM ATP (∼1 Ci mmol-1 [γ32P]-ATP) ...
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bioRxiv - Cell Biology 2023Quote: ... cells were treated for 2 h with 35 µM cycloheximide (Calbiochem, catalog no. 239763). For mTOR inhibition-dependent autophagy arrest at the initiation step ...
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bioRxiv - Cell Biology 2023Quote: The antibodies listed in Tables 1 and 2 were diluted as indicated in PBS containing 1% BSA (126575, Calbiochem).
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bioRxiv - Neuroscience 2022Quote: ... the cells were then incubated overnight at 4°C with primary antibodies (rabbit anti-AUTS2, 1:200, Atlas Antibodies; rabbit anti-GRIA1, 1:10, Calbiochem; mouse anti-GRIA2 ...
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bioRxiv - Molecular Biology 2022Quote: ... Samples were incubated for 1 h with the ADAM17 inhibitor Batimastat (BB-94) at 10 μM (196440, Calbiochem), with the selective ADAM10 inhibitor GI254023X (GI ...
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bioRxiv - Cell Biology 2023Quote: For IPA-3 (p21-Activated Kinas Inhibitor III, CAS 42521-82-4, Calbiochem), between 20 to 30 cells were first selected and optogenetically activated for 30 minutes ...
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bioRxiv - Cancer Biology 2021Quote: ... 2 Mm EDTA 1% Triton-X and1:400 protease-inhibitor mixture (Calbiochem)) ...
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bioRxiv - Cancer Biology 2022Quote: ... Transduced cells were selected with puromycin (Calbiochem; 1 to 2 µg/ml) or directly used in some superinfection experiments with pMRX-IP-GFP-LC3-RFP-LC3ΔG.
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bioRxiv - Cell Biology 2023Quote: ... Equivalent amounts of protein (1-2 mg) were incubated with 1 μg indicated antibody and 20 μL protein A/G beads (#IP05, Calbiochem) at 4 ℃ overnight ...
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bioRxiv - Cancer Biology 2022Quote: ... Immunodetection of RAS proteins was carried out with pan-RAS (Ab-3; Calbiochem; 1:1,000) antibodies ...
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bioRxiv - Cancer Biology 2023Quote: NMDI-14 inhibitor (Ethyl 2-(((6,7-dimethyl-3-oxo-1,2,3,4-tetrahydro-2-quinoxalinyl)acetyl)amino)-4,5-dimethyl-3-thiophenecarboxylate) was purchased from Calbiochem (Cat. Nr. 530838) and reconstituted in DMSO (10mg/ml ...
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bioRxiv - Neuroscience 2023Quote: ... protease inhibitors (PI, 1:4000 dilution of protease inhibitor cocktail 3 w/o EDTA, Calbiochem, #539134) and benzonase (1:1000 ...
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bioRxiv - Biophysics 2022Quote: ... were mixed in 70 μL of assembly buffer (50 mM HEPES-KOH, pH 7.5, 300 mM KCl, 5 mM MgCl2, 2 mM TCEP, 4% glycerol, 2 mM ATPγS [Calbiochem]) for 5 min at 25 °C ...
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bioRxiv - Immunology 2020Quote: ... 5-Nitro-2-(3-phenylpropylamino)benzoic acid (NPPB, 0593) was sourced from Calbiochem.
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bioRxiv - Cell Biology 2019Quote: ... 7-Amino-actinomycin D (Calbiochem) was added (Fig ...
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bioRxiv - Neuroscience 2021Quote: ... they were incubated for 2 h in secondary antibody (anti-mouse fluorescein conjugated, Calbiochem cat n. 401234) 1:100 in BSA 1% + sheep serum 2% in PBT ...
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bioRxiv - Neuroscience 2019Quote: ... the slices were treated with pronase (1 mg / 4-6 ml) (Calbiochem-MilliporeSigma, Burlington, MA, USA) in incubation medium I at 31°C for 30-50 min ...
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bioRxiv - Cancer Biology 2020Quote: ... Blots were incubated with following primary antibodies at 4 C overnight: TP53 (OP43, Calbiochem, 1:1000), RB1 (Ab-5 ...
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bioRxiv - Cell Biology 2024Quote: ... Membranes were incubated overnight at 4°C,with mouse anti-PGC-1α (Calbiochem, KP9803, 1:1000), mouse anti-PINK1 (Santa Cruz Biotechnology ...
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bioRxiv - Molecular Biology 2021Quote: ... The expression of recombinant PGC-1α proteins was induced for 3 h at 37°C with 0.4 mM isopropyl-β-D-thiogalactoside (IPTG, Calbiochem) in Terrific Broth and purified by metal ion affinity chromatography (IMAC ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... and the phenylacetamide compound (S)-2-(4-chlorophenyl)-3,3-dimethyl-N-(5-phenylthiazol-2-yl)butanamide (Cmp58) was obtained from Calbiochem-Merck Millipore (Billerica ...
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bioRxiv - Cell Biology 2020Quote: ... and the phenylacetamide compound (S)-2-(4-chlorophenyl)-3,3-dimethyl-N-(5-phenylthiazol-2-yl) butanamide (Cmp58) was obtained from Calbiochem-Merck Millipore (Billerica ...
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bioRxiv - Cell Biology 2020Quote: ... The allosteric FFAR2 modulator Cmp58 ((S)-2-(4-chlorophenyl)-3,3-dimethyl-N-(5-phenylthiazol-2-yl)butanamide was obtained from Calbiochem-Merck Millipore (Billerica ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... and the phenylacetamide compound (S)-2-(4-chlorophenyl)-3,3-dimethyl-N-(5-phenylthiazol-2-yl)butanamide (PA;Cmp58 [30]) was obtained from Calbiochem-Merck Millipore (Billerica ...
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bioRxiv - Genetics 2023Quote: ... Cells were passaged every 3-4 days using Accutase in presence of ROCKi (Y-27632, Calbiochem). VitC supplementation was performed as for mESCs.
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bioRxiv - Cell Biology 2020Quote: ... Abi1 [7] and GAPDH (6C5, Calbiochem). HRP-conjugated secondary antibodies were purchased from Invitrogen ...
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bioRxiv - Cell Biology 2020Quote: ... the following inhibitors were used at the indicated concentrations for 4 h unless indicated differently in the experiments: RO-3306 at 10 μM (CDK1 inhibitor; Calbiochem), NU6140 at 10 μM (CDK2 inhibitor ...
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bioRxiv - Cell Biology 2020Quote: Proteasome activity was quantitatively assessed in H4 cell extracts using the β5-selective fluorogenic substrate succinyl-leucine-leucine-valine-tyrosine-4-methyl-7-courmarylamide (Suc-LLVY-AMC, Calbiochem, Burlington, MA, USA) using an adapted protocol 87 ...
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bioRxiv - Cancer Biology 2019Quote: ... Membranes were incubated in 1:1000 diluted primary antibodies pERK1/2 pT202/Y204 (Calbiochem cat# KP26001), total ERK1/2 (Cell Signaling cat# 9102) ...
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bioRxiv - Immunology 2023Quote: ... 2 µl were used as PCR template for amplification with the KOD Polymerase (Calbiochem, 71086-3). The PCR products were cloned into the pI.18 vector ...
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bioRxiv - Developmental Biology 2021Quote: ... transferred to a 15 ml centrifuge tube containing warm 3 ml 0.5 mg ml−1 collagenase type II (Calbiochem, 234155) solution and incubated for 30 min at 37°C shaker at 180 rpm ...
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bioRxiv - Molecular Biology 2020Quote: ... Pellets were resuspended in 300 µl of DMEM 2% FBS 1 µg/mL propidium iodide (Calbiochem, 537060) and filtered through a 40-µm cell strainer (BD Falcon ...
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bioRxiv - Molecular Biology 2020Quote: ... Lyophilized MEISi-1 and MEISi-2 small molecules were dissolved in sterile dimethyl sulfoxide (DMSO, Calbiochem, 317275). Luciferase reporter assays were carried out as we have done previously 13,14,16,17 ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were rinsed with cold PBS1X and then lysed in Triton-X100 buffer at 4°C (50mM HEPES pH 7.4, 150mM NaCl, 1 % Triton X-100, protease inhibitors (Calbiochem) and phosphatase inhibitor cocktails II and IV (Calbiochem)) ...
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bioRxiv - Molecular Biology 2023Quote: ... 10 mM EDTA, 1% NP-40, 1% sodium deoxycholate, 0.1% SDS, 1 mM DTT, 5 mM PMSF, and 1:100 Calbiochem Complete Protease Inhibitor Cocktails VII and V ...
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bioRxiv - Cell Biology 2022Quote: ... wild-type mouse testes (n=3 per IP assay) were lysed in 1% NP-40 (PBS) supplemented with protease inhibitor cocktail (Calbiochem, 539134). KATNA1 ...
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bioRxiv - Cell Biology 2022Quote: ... and at 48 hpi cells transduced with Wasabi-pIPFCW2 were selected with 3-4 mg/ml puromycin (Calbiochem, 540411) and sorted for mid-range expression of Wasabi.
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bioRxiv - Neuroscience 2019Quote: ... or both hippocampi from a single mouse was homogenized in 2 mL of ice-cold homogenization buffer (0.32 M sucrose, 4 mM HEPES pH 7.4, protease inhibitor cocktail EGTA-free (Calbiochem, 1:1000), RNasin (Promega ...
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bioRxiv - Biochemistry 2022Quote: ... Nuclei were stained with DAPI (1:1000 in PBS, AppliChem, Darmstadt, Germany) and coverslips were mounted on Mowiol 4-88 (Calbiochem, Merck Millipore ...
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bioRxiv - Physiology 2021Quote: ... and phosphatase inhibitors (2 μM microcystin-LR, and 1× Phosphatase-Inhibitor Sets I and II (Calbiochem, Hayward, CA). We centrifuged homogenates at 4,000 g for 15 minutes to remove debris ...
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bioRxiv - Pathology 2023Quote: ... 2% DDM) supplemented with 1:1000 (v/v) Protease Inhibitor Cocktail Set III (Calbiochem, La Jolla, CA, USA). Rat anti-HA (3F10 ...
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bioRxiv - Immunology 2022Quote: ... 500 nM H-1152 (Calbiochem), 100 µg/ml penicillin and 100 µg/ml streptomycin ...
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bioRxiv - Cancer Biology 2019Quote: ... mice received 1 µg DT (322326-1, Calbiochem) diluted in 100 µl PBS through intraperitoneal (i.p. ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were washed 3 times with PBS and fixed to a slide using 5 μL of Mowiol (4-88 Reagent, Calbiochem). Fluorescent images were acquired with a Leica Dmi8 microscope (Leica Microsystems).
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bioRxiv - Molecular Biology 2020Quote: ... 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reagent was obtained from Calbiochem (San Diego, CA, USA).
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bioRxiv - Immunology 2022Quote: ... at a 4:1 molar ratio of monomer:streptavidin in the presence of a 1x protease inhibitor cocktail solution (Calbiochem, Millipore Sigma). 1/5th volumes of streptavidin-PE or streptavidin-BV421 were added to each monomer every 20 minutes and incubated ...
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bioRxiv - Immunology 2023Quote: ... at a 4:1 molar ratio of monomer:streptavidin in the presence of a 1x protease inhibitor cocktail solution (Calbiochem, Millipore Sigma). Briefly ...
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bioRxiv - Biochemistry 2023Quote: ... Ubiquitin was expressed as described above except that the 2 L culture was induced with 0.4 mM dioxane-free isopropyl B-D-1-thiogalactopyranoside (IPTG) (Calbiochem) for four hrs at 37℃ ...
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bioRxiv - Cell Biology 2023Quote: ... Vacuolin-1 (5 μM; Calbiochem, CAS 351986-85-1); Manumycin A (2,5 μM ...