Labshake search
Citations for Calbiochem :
701 - 750 of 789 citations for 6H Pyrazolo 3 4 b pyridin 6 one 3 cyclobutyl 1 2 4 5 tetrahydro 4 methyl 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cancer Biology 2022Quote: ... and phosphatase inhibitors at a dilution of 1: 500 (Calbiochem, Cat# 524625) for 10 minutes at 4 °C and then centrifuged at 14.000g for 15 minutes (Centrifuge 5415 R) ...
-
bioRxiv - Microbiology 2023Quote: ... or 1 µM of the synthetic Ffar2 agonist (#371725, Calbiochem, Merck Millipore). To inhibit Ffar2 ...
-
bioRxiv - Physiology 2024Quote: ... 0.5 mM Na3VO4 and 1x Protease Inhibitor Cocktail Set 1 (PIC) (Calbiochem). Lysates were mixed by pipetting and rotated at 4°C for 20 min then subjected to centrifugation (12000 g for 10 min at 4°C) ...
-
bioRxiv - Cell Biology 2020Quote: ... with a 1:1000 dilution of yeast protease inhibitor cocktail set IV (Calbiochem), the lysate was cleared by centrifugation at 4C at full speed on a microcentrifuge ...
-
bioRxiv - Molecular Biology 2020Quote: ... Primary antibodies were rabbit polyclonal anti-RAD51 (Abcam ab63801, 1:800 or Calbiochem PC130 ...
-
bioRxiv - Biochemistry 2020Quote: ... Bound antibodies were detected with peroxidase-labeled anti-rabbit IgG (1:30000) (Calbiochem), and ECL Prime Western Blotting Detection kit (GE Healthcare) ...
-
bioRxiv - Microbiology 2021Quote: ... Primary staining was performed for C3a with 1:2,000 rabbit anti-C3a (Calbiochem), for C5b6 with 1:500 dilution of goat-anti human C5 serum (Complement Technology ...
-
bioRxiv - Synthetic Biology 2022Quote: ... 10 mM imidazole and SetIII protease inhibitor-EDTA-free (1:1000 dilution, Calbiochem) were added ...
-
bioRxiv - Developmental Biology 2023Quote: ... Enzymatic reaction was blocked by adding PBS+10% FBS+1% DNAse (Calbiochem, 260913) and cell suspension was filtered and centrifuged at 1000 rpm for 5 min ...
-
bioRxiv - Biochemistry 2020Quote: ... and stable pools were selected with medium containing 500 µg ml-1 G418 (Calbiochem). The expression of the indicated genes in the stable pools was induced by the addition of 1 µg ml-1 doxycycline (Sigma ...
-
bioRxiv - Physiology 2021Quote: ... 0.1% sodium dodecyl sulfate) containing protease inhibitor cocktail (Cocktail set 1, Calbiochem, Bilerica, MA) and phosphatase inhibitors (25 mM NaF ...
-
bioRxiv - Neuroscience 2020Quote: ... Slices were incubated with polyclonal anti-tyrosine hydroxylase rabbit antibodies (1:1000, Calbiochem, Merck) and were incubated overnight in carrier solution (containing in PBS ...
-
bioRxiv - Biochemistry 2023Quote: ... Cells expressing the shRNA constructs were selected using 1 mg/mL Neomycin (Calbiochem, #480100). To induce expression of shRNAs cells were treated for 3 days with 2 µg/mL Dox.
-
bioRxiv - Molecular Biology 2020Quote: ... Cells were incubated with primary antibodies against RAD51 (rabbit anti-Rad51 1/200, Calbiochem PC130) and biotin (mouse anti-biotin 1/6,000 ...
-
bioRxiv - Neuroscience 2021Quote: ... Tissue samples were homogenized in TritonX-100 and protease inhibitor-containing (1:100, #539131, Calbiochem) Tris-HCl buffer (TBS ...
-
bioRxiv - Cell Biology 2021Quote: This study used Latrunculin-A at a final concentration of 1 µM (Calbiochem Cat# 428021), the PAK1 kinase inhibitor ...
-
bioRxiv - Neuroscience 2019Quote: ... Sections were incubated overnight (room temperature) with goat anti tyrosine hydroxylase (1:1000, Calbiochem/ Merck) antibody diluted in the carrier solution (0.2 M PBS with 1% horse serum ...
-
bioRxiv - Molecular Biology 2021Quote: ... 1% PenStrep) in the presence of selection antibiotic blasticidin S (Blast, 15 μg/ml, Calbiochem). Twenty-four h post plating ...
-
bioRxiv - Biochemistry 2021Quote: ... the cells were incubated with a blocking buffer (1% (w/v) bovine serum albumin (Calbiochem) and 0.5% (w/v ...
-
bioRxiv - Immunology 2023Quote: ... Tissue samples were homogenized in TritonX-100 and protease inhibitor-containing (1:100, #539131, Calbiochem) Tris-HCl buffer (TBS ...
-
bioRxiv - Cell Biology 2023Quote: ... The WAPL-AID cell line was supplemented with puromycin (0.5 µg mL-1, Calbiochem, 540411). HeLa SMC4-AID_WAPL-dTAG cells were supplemented with blasticidin S (6 µg mL-1 ...
-
bioRxiv - Molecular Biology 2020Quote: ... Cells were incubated for 1 h at room temperature with primary antibodies (rabbit anti-RAD51 Calbiochem PC130 1/200 and mouse anti-PCNA Santa Cruz PC10 1/500 ...
-
bioRxiv - Cell Biology 2021Quote: ... two other kinase inhibitors were used: 1 μM and 1.5 μM of AKT VIII (Calbiochem #124018) or 100 nM and 200 nM of trametinib (Selleckchem #GSK1120212) ...
-
bioRxiv - Neuroscience 2022Quote: ... Nuclei were counterstained with 1 µg/ml of DAPI and sections were mounted with Fluorsave (Calbiochem).
-
bioRxiv - Neuroscience 2021Quote: ... #MCA1957) and peptide-rich sensory nerve fibres with a rabbit anti-CGRP (1:2,000, Calbiochem, #PC205L). After washes ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... PamChip arrays (PamGene #88101) were blocked for 20 cycles with 1% Bovine serum albumin (Calbiochem #126609) in Tris-buffered saline (TBS) ...
-
bioRxiv - Developmental Biology 2023Quote: Cells were harvested in RIPA buffer with 1:100 protease inhibitor cocktail set I (Calbiochem, #539131) and phosphatase inhibitors (1:100 Na2Ov4 ...
-
bioRxiv - Cell Biology 2019Quote: ... cells were resuspended in KHM buffer containing CaCl2 (1 mM) and treated with Stapylocossus aureus nuclease (Calbiochem) (150 U/ml ...
-
bioRxiv - Molecular Biology 2019Quote: ... ATP regeneration system (1 mM ATP, 25 mM creatine monophosphate [Sigma], 0.03 U/µL creatine kinase [Calbiochem] ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... The master mix recipe contained polyclonal anti-rabbit anti-MLH1 (Calbiochem, San Diego CA; diluted 1:50) or anti-rabbit anti-DMC1 (mix of DMC1) ...
-
bioRxiv - Cell Biology 2022Quote: ... Cells were washed with ice cold PBS and lysed in IP lysis buffer (50 mM Tris, pH 7.5, 10 mM NaCl, 2.5 mM MgCl2, 1 mM DTT, 0.01% digitonin [Calbiochem 300410] and protease inhibitors ...
-
bioRxiv - Cell Biology 2023Quote: ... GFP-Rab11 WTlow and DNlow cell culture media was also supplemented with 1.25 μg.mL-1 puromycin (Calbiochem). Cells were regularly tested for mycoplasma contamination with the LookOut mycoplasma PCR detection kit (Sigma ...
-
bioRxiv - Microbiology 2023Quote: ... cells were pretreated 1 h at 37°C with 20 nM bafilomycin A1 (Calbiochem, stock in DMSO) to block endocytosis ...
-
bioRxiv - Neuroscience 2023Quote: ... The following primary antibodies were used: polyclonal rabbit anti-tyrosine hydroxylase (TH, catalog # 657012, 1:1000, Calbiochem), monoclonal mouse anti-TH (catalog # MAB318 ...
-
bioRxiv - Neuroscience 2023Quote: ... incubated overnight in TBST with 1% bovine serum albumin (BSA; Calbiochem by Millipore Sigma, St. Louis, MO) and primary antiserum (Millipore Sigma MAB318 ...
-
bioRxiv - Neuroscience 2019Quote: ... diluted in KPBS containing 0.3 % Triton X-100 and incubated overnight at RT in cFos rabbit polyclonal antiserum (1:20,000; Calbiochem) diluted in KPBS containing 1 % NGS and 0.3 % Triton X-100 ...
-
bioRxiv - Cell Biology 2020Quote: ... and the cells were washed with ice-cold PBS(-/-) with 1X Protease Inhibitor Cocktail Set 1 (Calbiochem 539131). The PBS was aspirated ...
-
bioRxiv - Neuroscience 2020Quote: ... 14 – 17 DIV hippocampi cultures were stained live with a GluA1 antibody (GluA1 Calbiochem PC246, rabbit, 1:20) for 15 min at 37°C in a humidified chamber ...
-
bioRxiv - Biochemistry 2021Quote: ... Cell pellets were sonicated in 20 mM HEPES buffer pH 7.5 containing 0.25 M sucrose and 1:100 protease inhibitor cocktail set V (Calbiochem) and centrifuged at 2,000 × g for 10 min to pellet nuclear fraction ...
-
bioRxiv - Biochemistry 2021Quote: ... Data were normalized to maximal inhibition determined by 1 μM decanoyl-Arg-Val-Lys-Arg-chloromethylketone (Calbiochem #344930).
-
bioRxiv - Neuroscience 2021Quote: ... or P5–P6 DRG rat neurons were diluted to 35,000 cells/ml in Sato’s media and treated with either 1 mM dbcAMP (Calbiochem), mixed population or recombinant Histones (1-20µg/ml ...
-
bioRxiv - Animal Behavior and Cognition 2022Quote: ... sections were incubated with rabbit anti-c-Fos antibody diluted with the blocking solution (1:20000, #PC38, Calbiochem) for 2 days at 4°C ...
-
bioRxiv - Molecular Biology 2022Quote: ... Samples were incubated for 1 h with the ADAM17 inhibitor Batimastat (BB-94) at 10 μM (196440, Calbiochem), with the selective ADAM10 inhibitor GI254023X (GI ...
-
bioRxiv - Biophysics 2023Quote: ... and the C-class iPSCs with a mixture of two inhibitors (2i): 1 μM MEK inhibitor (PD0325901, Calbiochem) and 3 μM GSK3 inhibitor (CH99021 ...
-
bioRxiv - Molecular Biology 2024Quote: ... The pellet was suspended in 2 ml of lysis/binding buffer (25 mM HEPES/KOH, pH 7.6, 10 % glycerol, 150 mM KCl, 1 mM dithiothreitol, 0.1 % Elugent (Calbiochem), loaded into SHREDDER tubes (Pressure Biosciences Inc ...
-
bioRxiv - Cell Biology 2019Quote: ... cells were lysed at 1 × 109 cells/ml in ice-cold lysis buffer (0.1 µM TLCK, 1 µg/ml Leupeptin, Phosphatase Inhibitor Cocktail II (Calbiochem),1 mM PMSF ...
-
bioRxiv - Biochemistry 2019Quote: ... which were grown to an optical density of 0.6–0.8 at 600 nm and were induced with 1 mM isopropyl-β-D-thiogalactopyranoside (CALBIOCHEM) for 20 h at 18 °C ...
-
bioRxiv - Cancer Biology 2022Quote: ... Cells were then harvested using lysis buffer (50mM HEPES pH7.4, 150mM NaCl, 1mM MgCl2, 1mM NaF, 1% Triton-x-100, protease inhibitor cocktail I [Calbiochem) and centrifuged at 14,000rpm for 10 minutes ...
-
bioRxiv - Cancer Biology 2022Quote: ... collected and lysed in lysis buffer RGMT (50mM HEPES pH 7.4, 150mM NaCl, 1mM MgCl2, 1mM NaF, 1% Triton-x-100, plus protease inhibitor cocktail I [Calbiochem]). Cellular lysates were cleared by centrifugation and 225µL of total protein extract was incubated with biotin-tagged CIGB-300 (100μM ...
-
bioRxiv - Molecular Biology 2022Quote: ... The cell pellet was lysed at 0.5 × 109 cells/ml in ice-cold lysis buffer (0.1 mM TLCK, 1 μg/ml Leupeptin, 1x Phosphatase Inhibitor Cocktail II tablet (Calbiochem), 1 mM PMSF ...