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Citations for Calbiochem :
551 - 600 of 609 citations for B TFIID TATA Box Binding Protein Associated Factor 1 BTAF1 Antibody Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... Cells were rinsed with cold PBS1X and then lysed in Triton-X100 buffer at 4°C (50mM HEPES pH 7.4, 150mM NaCl, 1 % Triton X-100, protease inhibitors (Calbiochem) and phosphatase inhibitor cocktails II and IV (Calbiochem)) ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were then harvested using lysis buffer (50mM HEPES pH7.4, 150mM NaCl, 1mM MgCl2, 1mM NaF, 1% Triton-x-100, protease inhibitor cocktail I [Calbiochem) and centrifuged at 14,000rpm for 10 minutes ...
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bioRxiv - Cancer Biology 2022Quote: ... collected and lysed in lysis buffer RGMT (50mM HEPES pH 7.4, 150mM NaCl, 1mM MgCl2, 1mM NaF, 1% Triton-x-100, plus protease inhibitor cocktail I [Calbiochem]). Cellular lysates were cleared by centrifugation and 225µL of total protein extract was incubated with biotin-tagged CIGB-300 (100μM ...
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bioRxiv - Molecular Biology 2022Quote: ... The cell pellet was lysed at 0.5 × 109 cells/ml in ice-cold lysis buffer (0.1 mM TLCK, 1 μg/ml Leupeptin, 1x Phosphatase Inhibitor Cocktail II tablet (Calbiochem), 1 mM PMSF ...
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bioRxiv - Developmental Biology 2022Quote: ... Tissues were homogenized in cold lysis buffer (0.1% NP-40, 50mM Tris-HCl [pH 7.2], 250mM NaCl, 2mM EDTA, phosphatase inhibitor mixture I and II [Calbiochem] and one tablet of Minicomplete [Roche] per 10ml ...
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bioRxiv - Immunology 2022Quote: ... and 1 × 106 cells were treated with rapamycin (10 ng/ml, 30 min, 37°C; Calbiochem, San Diego, CA) prior to treatment with rhGH.
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bioRxiv - Molecular Biology 2022Quote: ... pellets were resuspended in 800 μl of ice-cold wash buffer (50 mM NaH2PO4, 300 mM NaCl, 0.05% Tween, 1:200 diluted protease inhibitor cocktail set III (Calbiochem)) supplemented with 0.1 U/μL of recombinant RNase inhibitor (wash buffer-RIn ...
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bioRxiv - Neuroscience 2024Quote: ... 1 mM Na3VO4 and 500 nM Cantharidin (#3322.1 Roth)] and commercial protease inhibitors (protease inhibitor cocktail III, #539134, Calbiochem). Phosphatase inhibitors were omitted when Lambda phosphatase treatment was required ...
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bioRxiv - Molecular Biology 2023Quote: Whole-cell protein extracts were prepared using hot lysis buffer (1%SDS, 5mM EDTA, 50mM Tris, pH7.5) supplemented with protease inhibitor mixture (Calbiochem). Protein samples were diluted and boiled in 5X protein loading buffer (10% SDS ...
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bioRxiv - Biophysics 2023Quote: ... or FGFJI (20 ng ml−1, bFGF, R&D Systems; and 200 nM JAK inhibitor, Calbiochem, Merck Millipore, Germany). Cells were collected for mechanical characterization and RNA-Seq after 5-day exposure to the respective culture conditions (30).
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bioRxiv - Cancer Biology 2022Quote: ... Boston Bioproducts (Cat# BP-115X) supplemented with protease inhibitor cocktail at a dilution of 1: 100 (Calbiochem, Cat#539131) and phosphatase inhibitors at a dilution of 1 ...
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bioRxiv - Biochemistry 2023Quote: ... 0.2 mM DTT) supplemented with protease inhibitors (0.1 mM PMSF and 1:500 Cocktail IV (Calbiochem; San Diego, CA), and lysed by two passes through a French press at 1000 psi ...
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bioRxiv - Neuroscience 2023Quote: ... Heads were snap-frozen in liquid nitrogen then homogenized using a mechanical homogenizer in 100 μL TBS (50mM Tris-HCl, pH 8.0) containing 1:200 protease inhibitor cocktail set III (Calbiochem). Homogenates were centrifuged at 16,000 g at 4°C for 30 min ...
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bioRxiv - Immunology 2021Quote: ... GMDCs were incubated for 2 h at 37°C with 50 µM MARK inhibitor (MARK/Par-1 Activity Inhibitor, 39621; Calbiochem), 50 nM SIK inhibitor (HG-9-91-01 ...
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bioRxiv - Molecular Biology 2019Quote: ... 0.1 mM PMSF and 0.1 μM Aprotinin) using a Dounce homogenizer (10 strokes, 400 rpm) and filtered through two layers of Mira cloth (Calbiochem). The filtrate was centrifuged at 600g at 4° C for 2 min to remove crud and unbroken cells ...
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bioRxiv - Neuroscience 2019Quote: ... or both hippocampi from a single mouse was homogenized in 2 mL of ice-cold homogenization buffer (0.32 M sucrose, 4 mM HEPES pH 7.4, protease inhibitor cocktail EGTA-free (Calbiochem, 1:1000), RNasin (Promega ...
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bioRxiv - Immunology 2021Quote: The caspase-1 activity was determined with the fluorometric substrate Z-YVAD 7-Amido-4-trifluoromethylcoumarin (Z-YVAD-AFC, caspase-1 substrate VI, Calbiochem), as described previously (Angosto et al. ...
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bioRxiv - Cell Biology 2022Quote: ... and AR-C118925 {5-[[5-(2,8-dimethyl-5H-dibenzo[a,d]cyclohepten-5-yl)-3,4-dihydro-2-oxo-4-thioxo-1(2H)-pyrimidinyl]methyl]-N-2H-tetrazol-5-yl-2-furancarboxamide} were obtained from Calbiochem-Merck Millipore (Billerica ...
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bioRxiv - Cancer Biology 2022Quote: ... Whole cell samples were ground in liquid nitrogen and lysed in 8 M urea supplemented with 1% protease inhibitor cocktail (Calbiochem). Lysates were centrifuged at 12,000 x g and the supernatant was quantified by BCA assays (Beyotime) ...
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bioRxiv - Microbiology 2022Quote: ... The furin inhibitor decanoyl-RVKR-cmk and mannosidase-1 inhibitor deoxymannojirmycin (DMJ) were purchased from Calbiochem (San Diego, California, USA).
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bioRxiv - Developmental Biology 2021Quote: ... transferred to a 15 ml centrifuge tube containing warm 3 ml 0.5 mg ml−1 collagenase type II (Calbiochem, 234155) solution and incubated for 30 min at 37°C shaker at 180 rpm ...
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bioRxiv - Immunology 2022Quote: The caspase-1 activity was determined with the fluorometric substrate Z-YVAD 7-Amido-4-trifluoromethylcoumarin (Z-YVAD-AFC, caspase-1 substrate VI, Calbiochem), as described previously (Angosto et al. ...
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bioRxiv - Immunology 2019Quote: ... Sections were then incubated overnight in (4°C) with anti-c-ErbB2/c-Neu (Ab-3) mouse mAb (3B5) (1:500, Calbiochem). After washing with PBS ...
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bioRxiv - Immunology 2019Quote: ... bovine serum albumin in 0.1 M phosphate buffer for 1 h at room temperature and incubated overnight (4°C) with anti-c-ErbB2/c-Neu (Ab-3) mouse mAb (3B5) (1:500, Calbiochem) or anti-CD3 (1:500 ...
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bioRxiv - Cancer Biology 2020Quote: ... slides were counterstained with DAPI (1:50000 from 10 mg/ml stock solution, Serva Eletrophoresis) and embedded with geltol (Calbiochem). 2 representative pictures of 4 biological replicates per genotype were taken with LSM 5 Exciter (Zeiss ...
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bioRxiv - Cell Biology 2019Quote: ... Crosses were performed by random spore analysis (1, 2) followed by marker selection (Hygromycin/Kanamycin resistance, ura/leu auxotrophy, or sensitivity to 5 µM 1NM-PP1 (Calbiochem) for strains carrying the Ark1-as3 allele ...
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bioRxiv - Genetics 2020Quote: Drosophila larval brains were homogenized with pestles in RIPA buffer plus the addition of proteinase inhibitors 1:200 (539134, Calbiochem). The homogenate was centrifuged at 13000 rpm for 20 minutes at 4°C ...
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bioRxiv - Developmental Biology 2020Quote: Mammalian cells were lysed using RIPA buffer (50mM Tris, pH 7.4, 150mM NaCl, 1%NP-40, 0.5%sodium deoxycholate) with protease inhibitors (Calbiochem, Billerica, MA). Protein samples were separated on 4–15%gradient TGX precast gels (Bio-Rad ...
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bioRxiv - Cell Biology 2021Quote: ... the cell pellet was lysed by sonication in lysis buffer (2mM Tris–HCl pH 7.4, 0.5M NaCl, 20mM imidazole, 10% glycerol, Protease Inhibitor Cocktail Set III Calbiochem, 1:10,000) and centrifuged (10,000g ...
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bioRxiv - Immunology 2022Quote: ... Cells were left unstimulated or stimulated for indicated time at 37 °C with PMA (10 ng/ml; Alexis) and ionomycin (1 μM; Calbiochem) or solvent (DMSO) ...
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bioRxiv - Immunology 2022Quote: Caspase-1 activity was determined with the fluorometric substrate Z-YVAD 7-Amido-4-trifluoromethylcoumarin (Z-YVAD-AFC, caspase-1 substrate VI, Calbiochem) as described previously [47,48] ...
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bioRxiv - Biochemistry 2022Quote: ... Nuclei were stained with DAPI (1:1000 in PBS, AppliChem, Darmstadt, Germany) and coverslips were mounted on Mowiol 4-88 (Calbiochem, Merck Millipore ...
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bioRxiv - Neuroscience 2023Quote: ... mg of tissue/µl of NP-40 lysis buffer (20mM Tris- HCl pH 7.4, 150 mM NaCl, 100mM EDTA pH 7.4, 1% NP-40; Calbiochem Cat#492016) with protease inhibitors (cOmplete™ ...
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bioRxiv - Cell Biology 2023Quote: ... and cells were held for 16 hours in 1% Lipoprotein Deficient Serum (LDS) (Biomedical Technologies, BT907) and 25 μg/ml ALLN (Calbiochem) for 30 min prior to harvesting.
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bioRxiv - Cell Biology 2024Quote: ... Adh1 was probed with rabbit polyclonal primary antibody which was a gift from Claudio De Virgilio’s lab (Université de Fribourg, Switzerland) used with a dilution of 1:50,000 (Calbiochem ref 126745). The secondary antibody was Goat-anti-rabbit-HRP conjugate used with a dilution of 1:5000.
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bioRxiv - Microbiology 2024Quote: ... Viability was assessed after staining cells with 1 µg/mL propidium iodide (PI, stored at 4°C protected from light; Calbiochem). Cell number and percentage were obtained from gatings done on the Attune NxT software.
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bioRxiv - Immunology 2024Quote: ... Fexp are target+CTL samples and Flyse is the maximal target lysis in the presence of 1% Triton X-100 (Calbiochem). The γ value was measured at time zero ...
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bioRxiv - Cell Biology 2024Quote: ... 1% Triton X-100) supplemented with protease and phosphatase inhibitors (Protease inhibitor set III and Phosphatase inhibitor set II, Calbiochem), incubated on ice for 30 min ...
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Autophagy suppression in DNA damaged cells occurs through a newly identified p53-proteasome-LC3 axisbioRxiv - Cell Biology 2024Quote: ... monolayers of HCT116 cells were incubated at 37 °C and under 5% CO2 in the presence of Bleocin (0.1, 1, 10, 100 μg/mL, 5% H2O as the carrier control; #203408, Calbiochem, USA), Etoposide (5 μg/mL ...
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bioRxiv - Cell Biology 2019Quote: ... 2-3 × 105 single cells EpiSCs were seeded on 5 μg/10 cm2 fibronectin-coated 6-well plates in media containing (1 μl/ml) ROCK inhibitor (InSolution™ Y-276320, Cat.#688001-500UG, VWR Calbiochem). In the morning of transfection ...
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bioRxiv - Bioengineering 2022Quote: ... All the above stages were performed in cold phosphate-buffered saline (PBS) with 1 × Protease Inhibitor cocktail set I (Calbiochem 539,131). Eyes that were initially fixed in 10% NBF or 2.5% PFA were dissected to create eyecups ...
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bioRxiv - Immunology 2022Quote: ... at a 4:1 molar ratio of monomer:streptavidin in the presence of a 1x protease inhibitor cocktail solution (Calbiochem, Millipore Sigma). 1/5th volumes of streptavidin-PE or streptavidin-BV421 were added to each monomer every 20 minutes and incubated ...
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bioRxiv - Immunology 2023Quote: ... at a 4:1 molar ratio of monomer:streptavidin in the presence of a 1x protease inhibitor cocktail solution (Calbiochem, Millipore Sigma). Briefly ...
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bioRxiv - Molecular Biology 2023Quote: ... cultures were lysed with a solution containing SYBR green (1:5000, Invitrogen Cat. No. S7567) (Lysis buffer: 0.16% saponin (Calbiochem/EMD #558255), 0.05 M TRIS-HCl (Roche Cat ...
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bioRxiv - Genetics 2023Quote: ... Raw values were normalized by geometric mean and used for subsequent housekeeping normalization (α-tubulin values; 1:10,000 mouse anti-tubulin; Calbiochem CP06) from the same blot.
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bioRxiv - Cell Biology 2022Quote: ... wild-type mouse testes (n=3 per IP assay) were lysed in 1% NP-40 (PBS) supplemented with protease inhibitor cocktail (Calbiochem, 539134). KATNA1 ...
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bioRxiv - Molecular Biology 2024Quote: ... 0.1 mM EGTA, 1% Triton X-100, 0.1% sodium deoxycholate, 0.5% SLS, 0.1% SDS, 0.5 mM PMSF, Calbiochem Complete Protease Inhibitor Cocktail V). The suspension was incubated for 30 min at 4°C and then sonicated with Covaris ME220 focused-ultrasonicator (50 alternating 15-s ON and 45-s OFF intervals ...
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bioRxiv - Neuroscience 2019Quote: Pre-sorted synaptosomes (~750 μl) on iced were mixed with ice cold PBS containing protease inhibitor cocktail EGTA-free (Calbiochem, 1:1000) and RNasin (Promega ...
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bioRxiv - Bioengineering 2020Quote: ... Tested additives were added to the qRT-PCR reaction mix at the following concentrations: 1% w/v Tween-20 (Calbiochem 655204-100mL), 0.8 µg/µL BSA (Fisher BP675-1) ...
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bioRxiv - Cancer Biology 2020Quote: ... The 2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-one (PD98059, MEK inhibitor) and STAT3 inhibitor VI were from Calbiochem (Darmstadt, Germany). The EGF was from Millipore (Billerica ...