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Citations for Calbiochem :
501 - 550 of 614 citations for 1 6 Chloropyridazin 3 yl piperidine 4 carboxamide since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... MARK/Par-1 activity inhibitor49 (MARK2i; Calbiochem 39621; 5 µM or 10 µM) was added prior to imaging ...
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bioRxiv - Molecular Biology 2020Quote: ... Primary antibodies were rabbit polyclonal anti-RAD51 (Abcam ab63801, 1:800 or Calbiochem PC130 ...
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bioRxiv - Biochemistry 2020Quote: ... Bound antibodies were detected with peroxidase-labeled anti-rabbit IgG (1:30000) (Calbiochem), and ECL Prime Western Blotting Detection kit (GE Healthcare) ...
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bioRxiv - Microbiology 2021Quote: ... Primary staining was performed for C3a with 1:2,000 rabbit anti-C3a (Calbiochem), for C5b6 with 1:500 dilution of goat-anti human C5 serum (Complement Technology ...
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bioRxiv - Developmental Biology 2023Quote: ... Enzymatic reaction was blocked by adding PBS+10% FBS+1% DNAse (Calbiochem, 260913) and cell suspension was filtered and centrifuged at 1000 rpm for 5 min ...
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bioRxiv - Synthetic Biology 2022Quote: ... 10 mM imidazole and SetIII protease inhibitor-EDTA-free (1:1000 dilution, Calbiochem) were added ...
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bioRxiv - Neuroscience 2024Quote: ... or CC1 (anti-adenomatous polyposis coli clone CC1, ref. OP80, Calbiochem, 1/200) and a 2-hour incubation with the secondary fluorophore-conjugated antibody ...
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bioRxiv - Immunology 2024Quote: ... Target cells alone and target cells lysed with 1% Triton X-100 (Calbiochem) were used for instrument set up and gating CFSE+PI+ (Fig ...
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bioRxiv - Microbiology 2024Quote: ... proteinase K was inactivated by addition of 1 mM phenylmethanesulfonyl fluoride (Calbiochem, 7110). Cells were lysed by addition of 1% Triton X-100 followed by incubation at room temperature for 15 min ...
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bioRxiv - Biophysics 2024Quote: ... and 1:1,000 dilution of Protease Inhibitor Cocktail Set III (EDTA free, Calbiochem) supplemented with 10 mM of the detergent LMNG (Anatrace) ...
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bioRxiv - Biochemistry 2020Quote: ... and stable pools were selected with medium containing 500 µg ml-1 G418 (Calbiochem). The expression of the indicated genes in the stable pools was induced by the addition of 1 µg ml-1 doxycycline (Sigma ...
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bioRxiv - Physiology 2021Quote: ... 0.1% sodium dodecyl sulfate) containing protease inhibitor cocktail (Cocktail set 1, Calbiochem, Bilerica, MA) and phosphatase inhibitors (25 mM NaF ...
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bioRxiv - Neuroscience 2020Quote: ... Slices were incubated with polyclonal anti-tyrosine hydroxylase rabbit antibodies (1:1000, Calbiochem, Merck) and were incubated overnight in carrier solution (containing in PBS ...
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bioRxiv - Biochemistry 2023Quote: ... Cells expressing the shRNA constructs were selected using 1 mg/mL Neomycin (Calbiochem, #480100). To induce expression of shRNAs cells were treated for 3 days with 2 µg/mL Dox.
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bioRxiv - Microbiology 2023Quote: ... diluted 5-fold in 100% MeOH containing 1 µM glycocholic acid (Calbiochem, 100.1% pure), and centrifuged for 10 min at 4000 RPM (Thermo Sorvall ST 40R ...
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bioRxiv - Microbiology 2024Quote: ... diluted 10-fold in 50% MeOH containing 1 µM glycocholic acid (Calbiochem, 100.1% pure), and centrifuged for 10 min at 4000 RPM (Thermo Sorvall ST 40R ...
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bioRxiv - Molecular Biology 2020Quote: ... Cells were incubated with primary antibodies against RAD51 (rabbit anti-Rad51 1/200, Calbiochem PC130) and biotin (mouse anti-biotin 1/6,000 ...
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bioRxiv - Neuroscience 2021Quote: ... Tissue samples were homogenized in TritonX-100 and protease inhibitor-containing (1:100, #539131, Calbiochem) Tris-HCl buffer (TBS ...
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bioRxiv - Cell Biology 2021Quote: This study used Latrunculin-A at a final concentration of 1 µM (Calbiochem Cat# 428021), the PAK1 kinase inhibitor ...
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bioRxiv - Molecular Biology 2021Quote: ... 1% PenStrep) in the presence of selection antibiotic blasticidin S (Blast, 15 μg/ml, Calbiochem). Twenty-four h post plating ...
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bioRxiv - Neuroscience 2021Quote: ... One replicate of each sample was treated with 1 mM conduritol B epoxide (CBE, Calbiochem) for 10 min before all samples were incubated for 1 hr with 2.5 mM of the fluorescent GCase substrate methylumbillifery β-D-glucopyranoside (4MUG ...
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bioRxiv - Biochemistry 2021Quote: ... the cells were incubated with a blocking buffer (1% (w/v) bovine serum albumin (Calbiochem) and 0.5% (w/v ...
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bioRxiv - Immunology 2023Quote: ... Tissue samples were homogenized in TritonX-100 and protease inhibitor-containing (1:100, #539131, Calbiochem) Tris-HCl buffer (TBS ...
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bioRxiv - Cell Biology 2023Quote: ... The WAPL-AID cell line was supplemented with puromycin (0.5 µg mL-1, Calbiochem, 540411). HeLa SMC4-AID_WAPL-dTAG cells were supplemented with blasticidin S (6 µg mL-1 ...
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bioRxiv - Cell Biology 2024Quote: ... 1000 ng of previously purified IqgC [55] was added to 1 μM H-Ras (Calbiochem) as a control or to purified GST-RapA(wt ...
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bioRxiv - Immunology 2024Quote: ... 1% Triton X-100 in the presence of Protease Inhibitor Cocktail Set III (Calbiochem®) and 0.2 mg sodium orthovanadate for 5 min on ice ...
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bioRxiv - Molecular Biology 2024Quote: ... The resistant clone expressing LOX-1 and mutated AT1 in response to doxycycline (Calbiochem, USA) was selected for use in the experiments (CHO-LOX-1-AT1mg ...
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bioRxiv - Neuroscience 2024Quote: ... probed with horseradish peroxidase-conjugated anti-rabbit or anti-mouse secondary antibodies (1:10,000, Calbiochem) and developed using chemiluminescence reagents (ECL ...
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bioRxiv - Cancer Biology 2022Quote: ... ZBTB18 or ZBTB18-mut as described above or treated with 5 µM RN-1 inhibitor (Calbiochem), for 96h ...
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bioRxiv - Molecular Biology 2020Quote: ... Cells were incubated for 1 h at room temperature with primary antibodies (rabbit anti-RAD51 Calbiochem PC130 1/200 and mouse anti-PCNA Santa Cruz PC10 1/500 ...
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bioRxiv - Cell Biology 2021Quote: ... two other kinase inhibitors were used: 1 μM and 1.5 μM of AKT VIII (Calbiochem #124018) or 100 nM and 200 nM of trametinib (Selleckchem #GSK1120212) ...
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bioRxiv - Neuroscience 2022Quote: ... Nuclei were counterstained with 1 µg/ml of DAPI and sections were mounted with Fluorsave (Calbiochem).
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bioRxiv - Neuroscience 2021Quote: ... #MCA1957) and peptide-rich sensory nerve fibres with a rabbit anti-CGRP (1:2,000, Calbiochem, #PC205L). After washes ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... PamChip arrays (PamGene #88101) were blocked for 20 cycles with 1% Bovine serum albumin (Calbiochem #126609) in Tris-buffered saline (TBS) ...
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bioRxiv - Developmental Biology 2023Quote: Cells were harvested in RIPA buffer with 1:100 protease inhibitor cocktail set I (Calbiochem, #539131) and phosphatase inhibitors (1:100 Na2Ov4 ...
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bioRxiv - Molecular Biology 2024Quote: ... 1% Triton X-100 lysis buffer supplemented with protease inhibitors (539134, Calbiochem, San Diego, CA, USA), and collected by scrapping ...
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bioRxiv - Biochemistry 2024Quote: ... 10 mM Imidazole (Buffer A) supplemented with 1:1000 protease inhibitors (Calbiochem Set III, Merck 539134), 3 µg/mL Benzonase-MBP ...
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bioRxiv - Molecular Biology 2020Quote: ... Pellets were resuspended in 300 µl of DMEM 2% FBS 1 µg/mL propidium iodide (Calbiochem, 537060) and filtered through a 40-µm cell strainer (BD Falcon ...
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bioRxiv - Molecular Biology 2020Quote: ... Lyophilized MEISi-1 and MEISi-2 small molecules were dissolved in sterile dimethyl sulfoxide (DMSO, Calbiochem, 317275). Luciferase reporter assays were carried out as we have done previously 13,14,16,17 ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The master mix recipe contained polyclonal anti-rabbit anti-MLH1 (Calbiochem, San Diego CA; diluted 1:50) or anti-rabbit anti-DMC1 (mix of DMC1) ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were washed with ice cold PBS and lysed in IP lysis buffer (50 mM Tris, pH 7.5, 10 mM NaCl, 2.5 mM MgCl2, 1 mM DTT, 0.01% digitonin [Calbiochem 300410] and protease inhibitors ...
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bioRxiv - Cell Biology 2023Quote: ... Controls were treated for 30 minutes with KRBH + 2.8 mM glucose + 1 μM latrunculin B (Calbiochem, #428020) or 1 μM jasplakinolide (Millipore Sigma ...
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bioRxiv - Cell Biology 2023Quote: ... GFP-Rab11 WTlow and DNlow cell culture media was also supplemented with 1.25 μg.mL-1 puromycin (Calbiochem). Cells were regularly tested for mycoplasma contamination with the LookOut mycoplasma PCR detection kit (Sigma ...
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bioRxiv - Neuroscience 2023Quote: ... The following primary antibodies were used: polyclonal rabbit anti-tyrosine hydroxylase (TH, catalog # 657012, 1:1000, Calbiochem), monoclonal mouse anti-TH (catalog # MAB318 ...
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bioRxiv - Microbiology 2023Quote: ... cells were pretreated 1 h at 37°C with 20 nM bafilomycin A1 (Calbiochem, stock in DMSO) to block endocytosis ...
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bioRxiv - Neuroscience 2024Quote: ... incubated overnight in TBST with 1% bovine serum albumin (BSA; Calbiochem by Millipore Sigma, St. Louis, MO) and primary antiserum (Millipore Sigma MAB318 ...
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bioRxiv - Biochemistry 2024Quote: ... 1 mM phenylmethane sulfonyl fluoride (PMSF) and Protease Inhibitor Cocktail Set III (Calbiochem, San Diego, CA, USA). The cells were disrupted in an EmulsiFlex C3 homogenizer (Avestin Inc. ...
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bioRxiv - Cell Biology 2020Quote: ... and the cells were washed with ice-cold PBS(-/-) with 1X Protease Inhibitor Cocktail Set 1 (Calbiochem 539131). The PBS was aspirated ...
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bioRxiv - Neuroscience 2020Quote: ... 14 – 17 DIV hippocampi cultures were stained live with a GluA1 antibody (GluA1 Calbiochem PC246, rabbit, 1:20) for 15 min at 37°C in a humidified chamber ...
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bioRxiv - Physiology 2021Quote: ... and phosphatase inhibitors (2 μM microcystin-LR, and 1× Phosphatase-Inhibitor Sets I and II (Calbiochem, Hayward, CA). We centrifuged homogenates at 4,000 g for 15 minutes to remove debris ...